IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ soba-2005-1 Homo- and heterodimerization of APP family members promotes intercellular adhesion. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease soba-2005-2 Homo- and heterodimerization of APP family members promotes intercellular adhesion. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease soba-2005-3 Homo- and heterodimerization of APP family members promotes intercellular adhesion. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease soba-2005-4 Homo- and heterodimerization of APP family members promotes intercellular adhesion. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease p05067-4 Amyloid-beta precursor protein APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI PreA4 695 Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN A major isoform. aplp1_human Amyloid beta precursor like protein 1 APLP1 Amyloid beta (A4) precursor-like protein 1 Amyloid-like protein 1 protein protein human Homo sapiens Human MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLTLEEQQLRELQRHGYENPTYRFLEERP aplp2_human Amyloid beta precursor like protein 2 APPH APPL2 APLP2 Amyloid protein homolog CDEI box-binding protein Amyloid beta (A4) precursor-like protein 2 Amyloid-like protein 2 Sperm membrane protein YWK-II protein protein human Homo sapiens Human MAATGTAAAAATGRLLLLLLVGLTAPALALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEEVLQYCQEMYPELQITNVMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFHKERMEVCENHQHWHTVVKEACLTQGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEENPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCKAMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRMALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTASISETPVDVRVSSEESEEIPPFHPFHPFPALPENEDTQPELYHPMKKGSGVGEQDGGLIGAEEKVINSKNKVDENMVIDETLDVKEMIFNAERVGGLEEERESVGPLREDFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI aplp1_mouse Amyloid beta precursor like protein 1 Amyloid-like protein 1 Amyloid beta (A4) precursor-like protein 1 Aplp1 protein protein mouse Mus musculus Mouse MGPTSPAARGQGRRWRPPPLPLLLPLSLLLLRAQLAVGNLAVGSPSAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLLDPQRVLEYCRQMYPELHIARVEQAAQAIPMERWCGGTRSGRCAHPHHEVVPFHCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPATPNPSGMAAGDPSTRSWPLGGRAEGGEDEEEVESFPQPVDDYFVEPPQAEEEEEEEEERAPPPSSHTPVMVSRVTPTPRPTDGVDVYFGMPGEIGEHEGFLRAKMDLEERRMRQINEVMREWAMADSQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQGDPPQAERVLMALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVQTHLQVIEERMNQSLGLLDQNPHLAQELRPQIQELLLAEHLGPSELDASVPGSSSEDKGSLQPPESKDDPPVTLPKGSTDQESSSSGREKLTPLEQYEQKVNASAPRGFPFHSSDIQRDELAPSGTGVSREALSGLLIMGAGGGSLIVLSLLLLRKKKPYGTISHGVVEVDPMLTLEEQQLRELQRHGYENPTYRFLEERP a4_mouse Amyloid-beta precursor protein App Alzheimer disease amyloid A4 protein homolog ABPP Amyloidogenic glycoprotein Amyloid precursor protein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein mouse Mus musculus Mouse MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSVDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATERTTSTATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVSTQSLLKTTSEPLPQDPDKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN aplp2_mouse Amyloid beta precursor like protein 2 APPL2 Amyloid beta (A4) precursor-like protein 2 Amyloid protein homolog Amyloid-like protein 2 Sperm membrane protein YWK-II CDEI box-binding protein Aplp2 protein protein mouse Mus musculus Mouse MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKRQCKSHIVIPFKCLVGEFVSDVLLVPDNCQFFHQERMEVCEKHQRWHTLVKEACLTEGLTLYSYGMLLPCGVDQFHGTEYVCCPQTKTVDSDSTMSKEEEEEEEDEEDEEEDYDLDKSEFPTEADLEDFTEAAADEEEEDEEEGEEVVEDRDYYYDPFKGDDYNEENPTEPSSEGTISDKEIVHDVKVPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPKTERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPLHPFPSLSENEDTQPELYHPMKKGSGMAEQDGGLIGAEEKVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPESVGPLREDFSLSSNALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI app-app-1 myc tagged APP interacts with HA tagged APP expressed in COS-7 cells 740455 n/a 740459 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740459 unspecified role unspecified role prey prey app e1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp1-aplp1 myc tagged APLP1 interacts with HA tagged APLP1 expressed in COS-7 cells 740455 n/a 740460 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740460 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false e1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp2-aplp2 myc tagged APLP2 interacts with HA tagged APLP2 expressed in COS-7 cells 740455 n/a 740461 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false e1 domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740461 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp1-app myc tagged APLP1 interacts with HA tagged APP expressed in COS-7 cells 740455 n/a 740459 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 740460 unspecified role unspecified role prey prey n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 app-aplp2 myc tagged APLP2 interacts with HA tagged APP expressed in COS-7 cells 740455 n/a 740461 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false e1 region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740459 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp2-aplp1 myc tagged APLP2 interacts with HA tagged APLP1 expressed in COS-7 cells 740455 n/a 740460 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740461 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false e1 region required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp1-app-4 myc tagged APLP1 interacts with HA tagged APP expressed in COS-7 cells 740455 n/a 740459 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740460 unspecified role unspecified role bait bait n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 aplp2-aplp1-1 myc tagged APLP2 interacts with HA tagged APLP1 expressed in COS-7 cells 740455 n/a 740461 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740460 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 app-aplp2-2 myc tagged APLP2 interacts with HA tagged APP expressed in COS-7 cells 740455 n/a 740459 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740461 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 app-app-3 myc tagged APP interacts with HA tagged APP expressed in COS-7 cells 740455 n/a 740459 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 740459 unspecified role unspecified role bait bait app e1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp1-aplp1-2 myc tagged APLP1 interacts with HA tagged APLP1 expressed in COS-7 cells 740455 n/a 740460 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740460 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 aplp2-aplp2-2 myc tagged APLP2 interacts with HA tagged APLP2 expressed in COS-7 cells 740455 n/a 740461 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 740461 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 app-app-2 APP forms a homodimer at the cell surface in COS-7 cells 740456 n/a 740459 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 740459 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig 3 aplp1-aplp1-1 APLP1 forms a homodimer at the cell surface in COS-7 cells 740456 N/A 740460 unspecified role unspecified role unspecified role unspecified role region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 3 aplp2-aplp2-1 APLP2 forms a homodimer at the cell surface in COS-7 cells 740456 n/a 740461 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 740461 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig 3 aplp1-app-1 APLP1 interacts with APP in mouse brain extracts. 740457 n/a 740462 unspecified role unspecified role bait bait n/a 740463 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig 6 C-terminal antibody 57 used to immunoprecipitate APLP1. At least 3 distinct forms of APP with molecular mass of 100, 108, and 120 KDa were detected likely representing different post-translationally modified APP 695 species. Only the 108 and 120 forms were coimmunoprcipated with APLP1. app-aplp2-1 APLP2 interacts with APP in mouse brain extracts. 740457 n/a 740464 unspecified role unspecified role prey prey n/a 740463 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig 6 APP immunoprecipated with antibody 22734. APLP2 only detected in coimmunoprecipates from WT and APLP1-/- brains. aplp1-aplp2 APLP2 interacts with APLP1 in mouse brain extracts. 740457 n/a 740464 unspecified role unspecified role prey prey n/a 740462 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig 6 APLP1 immunoprecipated with antibody 57. APLP2 only detected in coimmunoprecipates from WT and APP-/- brains. Only the mature form (120kDa) of APLP2 coimmunoprecipitated with APLP1. aplp1-app-2 APLP1 interacts with APP in synaptic compartment purified from mouse brain. 740458 n/a 740463 unspecified role unspecified role prey prey n/a 740462 unspecified role unspecified role bait bait physical association physical association false false false Fig 7C C-terminal antibody 57 used to immunoprecipitate APLP1. 108, and 120 KDa forms of APP specifically coimmunoprecipated.