IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fukuda-2004-1 Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells. in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip full dna sequence full identification by DNA sequencing full dna sequence Fukuda M., Kanno E., Yamamoto A. Synapse - Interactions of proteins with an established role in the presynapse. mnfukuda@brain.riken.go.jp J. Biol. Chem. (0021-9258) 2004 q9w2r9_drome Rph Dmel\CG11556 rph CG11556 Dmel_CG11556 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDFQNRNNTANKFVCPSDRQLALRAKLKAGWSSSKTSEPLRPEEQEAIISVIRRNEEIEVAERQRVGRLVERVEKIKQHAVERGPNCCRLCGDTFGILRPQRILCEDCRQSVCTKCSVDINIRYHTSERSREIWLCRICSETREMWKKSGAWFFKGLPKYDMPRSASATPIPNPGPGSMAGDTRAVQSCHATPTRPARVKKLTIRVNDSSSSSSGHSEPEDEVDTGVGIGVGVVGGGMAKGIASTRLQREDSFRLRAYGSIRSFIDGGERKLSNSFFFNRQQSQRPSECPEYDSVSMSKLRRESSFLRRGSVSSSWSISDSSGSGSNNSGNSQTQSQYQQHQQQLQQHCRDPLLGWLEIAISYREAFHSLDCTMVRARDLPAMDAAGLADPYCKLNIITPEAHTKYTRWQRTKTVHKTRNPEFNETLQFVGVEPEELGNSLIYVALFDDDKYGHDFLGAAKVCLSTVHSTSQYRISVPLGVEDQYSNAAEMAQNWPNGKMLLSLCYNTKRRALVVNVKQCINLMAMDNNGSSDPFVKIQLKPDAHKNKKHKTSVKWRTLNPIYNEEFYFEASPHDLNKEMLILTVWDKDLGKSNDFLGSLQLGAQSKGERLQQWLDCIRLPDHFHEKWHCLAPDNPAH o76901_drome Rab27 EG:80H7.4 Rab27-RB CG14791 18543235 AAF45634 AAF45635 DmRab27 Dmel\CG14791 O76901 dm-Rab27 rab27 Dmel_CG14791 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MRAAPPEPEPLQLAGSGEQFLVLGDSGVGKTCLLYQYTDGRFHTQFISTVGIDFREKRLLYNSRGRRHRIHLQIWDTAGQERFRSLTTAFYRDAMGFLLIFDLTSEKSFLETANWLSQLRTHAYSEDPDVVLCGNKCDLLQLRVVSRDQVAALCRRYRLPYIETSACTGANVKEAVELLVGRVMERIENAACNREFSLLLTQSRCLPNIAYGQPEDLVRLHDRREEPCSRRNCRNC rbf1_caeel Rabphilin-1 rbf-1 F37A4.7 protein protein caeel Caenorhabditis elegans MTKSTKLRHCKQKKKKPEKTPKGNPPILITEKSIEDAATTTSTTDALLGSPEGSRSKSRKLKLCCCTAQAATLSPLDPTSYGGIASTSAHNGMVGGLSRDSRAASRTSKRGSSKSLNRPQIDADEPSTSGTNPDRRPSTHFVLDLPVVSTRYLMNDWEIGGTQNKWVCPSDRHLHLRAQLKSGWSVRTATARSPTNSKAQTGSITAAEQEHIQKVLAKAEESKSKEQQRIGKMVDRLEKMRRRATGNGVTHCLLCHTEFGLLASKSYAAMCVDCRKYVCQRNCGVETTDVNQTTGKVETVFLCKICSEAREVLWKKSGAWFYKEMPEFQRPDDRLPYYVPVTTNGTLPNASSAATPLSGTPGGAGPQPMTMPSTSSCQMTTPKWASPGVCNSPGLQMNGGPTSPLPNGTRRNTGHGGIEFPSSSRPSICSVLQAIEPLDRSKSPRPRIQPRWVNEKVMSSMSVDDEEKAASSSDGESFVQSGVPRRALNNKTPVGSTSATTSPAPPPTSTTPTSRREANMERFSRHTHAHANRLYSTDDDDDSSPESRPSTRSTSPRHSLATPSSYAHDTCHDTSLPDADTRSIDSGVVQSDHSNPQQSGLTCSSSSLTPLQQQASHDHHSGGGTPRRISNPDRTTSRVAQSASGTSLVTPPPPISSRTSPDNCNSSPLNVMEHKSSSASTASSGGNRRVGSAEPVLNNHHAMHNNQNHNDINKKLISQTSRAESPLAASSSFLSSPDDDTKQKNRRRDGVGRVNSLQLRTSLDDVAPPVAPISKMNGHIVSSEPTSSTTSNQNHTSVPIPTVPVVPEEEEEKAITASTESASEPGSLGSITLTLTYHSADKKLKMHLIRAKNLKAMDSNGFSDPYVKFHLLPGNTKATKLTSKTIEKTLNPEWNEEMSYYGITEDDKEKKILRVTVLDRDRIGSDFLGETRIALKKLNDNEMKKFNLYLESALPVPQQTKEEENEDRGKINVGLQYNIQQGSLFININRCVELVGMDSTGFSDPYCKVSLTPITSKAHRAKTSTKKRTLNPEWNEQLQFVVPFKDLPKKTLQIGVYDHDLGKHDDYIGGILLSTSAKDERGRQWIKCIENPGTLVEAWHRLELDS g5ebs5_caeel Small monomeric GTPase CELE_Y87G2A.4 rab27 aex-6 Y87G2A.4 protein protein caeel Caenorhabditis elegans MGDYDYLIKFLALGDSGVGKTSFLHRYTDNTFTGQFISTVGIDFKEKKVVYKSSRGGFGGRGQRVLLQLWDTAGQERFRSLTTAFFRDAMGFILIFDITNEQSFLNIRDWLSQLKVHAYCEQPDIIICGNKADLENRRQVSTARAKQLADQLGLPYFETSACTSTNVEKSVDCLLDLVMQRIQQSVETSSLPLSECRGVSLDGDPSAASSYCANC rp3a_mouse Rabphilin-3A Rph3a Exophilin-1 protein protein mouse Mus musculus Mouse MTDTVVNRWMYPGDGPLQSNDKEQLQAGWSVHPGAQTDRQRKQEELTDEEKEIINRVIARAEKMEAMEQERIGRLVDRLETMRKNVAGDGVNRCILCGEQLGMLGSACVVCEDCKKNVCTKCGVETSNNRPHPVWLCKICLEQREVWKRSGAWFFKGFPKQVLPQPMPIKKTKPQQPAGEPATQEQPTPESRHPARAPARGDMEDRRPPGQKPGPDLTSAPGRGSHGPPTRRASEARMSTAARDSEGWDHAHGGGTGDTSRSPAGLRRANSVQAARPAPAPVPSPAPPQPVQPGPPGGSRATPGPGRFPEQSTEAPPSDPGYPGAVAPAREERTGPAGGFQAAPHTAAPYSQAAPARQPPPAEEEEEEANSYDSDEATTLGALEFSLLYDQDNSNLQCTIIRAKGLKPMDSNGLADPYVKLHLLPGASKSNKLRTKTLRNTRNPVWNETLQYHGITEEDMQRKTLRISVCDEDKFGHNEFIGETRFSLKKLKANQRKNFNICLERVIPMKRAGTTGSARGMALYEEEQVERIGDIEERGKILVSLMYSTQQGGLIVGIIRCVHLAAMDANGYSDPFVKLWLKPDMGKKAKHKTQIKKKTLNPEFNEEFFYDIKHSDLAKKSLDISVWDYDIGKSNDYIGGCQLGISAKGERLKHWYECLKNKDKKIERWHQLQNENHVSSD rab3a_mouse Ras-related protein Rab-3A Rab3a protein protein mouse Mus musculus Mouse MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLTDQQAPPHQDCAC rab8a_mouse Ras-related protein Rab-8A Rab8a Mel Rab8 Oncogene c-mel protein protein mouse Mus musculus Mouse MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL rb27a_mouse Ras-related protein Rab-27A Rab27a protein protein mouse Mus musculus Mouse MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRANGPDGAVGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRAVKEEEARELAEKYGIPYFETSAANGTNISHAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHTSADQLSEEKEKGLCGC rph-rab27-1 Rabphilin specifically interacts with Rab27 164269 N/A 164270 unspecified role unspecified role bait bait rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells N/A 164271 unspecified role unspecified role prey prey rbd domain region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells physical association physical association false false false Rabphilin does not interact with Rab3 and Rab8, contrary to the mouse Rabphilin rbf1-rab27-1 Rabphilin specifically interacts with Rab27 164269 N/A 164272 unspecified role unspecified role bait bait rbd domain region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells N/A 164273 unspecified role unspecified role prey prey c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells physical association physical association false false false Rabphilin does not interact with Rab3 and Rab8, contrary to the mouse Rabphilin rph3a-rab3a-3 Rabphilin interacts with Rab3, Rab8 and Rab27 164269 N/A 164274 unspecified role unspecified role bait bait rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells N/A 164275 unspecified role unspecified role prey prey c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells physical association physical association false false false rph3a-rab8a-2 Rabphilin interacts with Rab3, Rab8 and Rab27 164269 N/A 164276 unspecified role unspecified role prey prey c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells N/A 164274 unspecified role unspecified role bait bait rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells physical association physical association false false false rph3a-rab27a-2 Rabphilin interacts with Rab3, Rab8 and Rab27 164269 N/A 164277 unspecified role unspecified role prey prey c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells N/A 164274 unspecified role unspecified role bait bait rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells physical association physical association false false false