UniProt UniProt http://www.uniprot.org calero-2002a-1 Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Calero M., Collins RN. Synapse - Interactions of proteins with an established role in the presynapse. rnc8@cornell.edu Biochem. Biophys. Res. Commun. 290 (676-681) 2002 Only protein-protein interactions imex curation calero-2002a-2 Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Calero M., Collins RN. Synapse - Interactions of proteins with an established role in the presynapse. rnc8@cornell.edu Biochem. Biophys. Res. Commun. 290 (676-681) 2002 Only protein-protein interactions imex curation ypt6_yeast GTP-binding protein YPT6 L8479.11 YLR262C YPT6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRSGKSLTKYKIVFLGEQGVGKTSLITRFMYDTFDDHYQATIGIDFLSKTMYLDDKTIRLQLWDTAGQERFRSLIPSYIRDSRVAIIVYDITKRKSFEYIDKWIEDVKNERGDENVILCIVGNKSDLSDERQISTEEGEKKAKLLGAKIFMETSTKAGYNVKALFKKIAKSLPEFQNSESTPLDSENANSANQNKPGVIDISTAEEQEQSACQC A8E6211FF24FAE0B pra1_yeast Prenylated Rab acceptor 1 YIP3 PRA1 YNL044W N2650 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNQLGALAQVSRFTQNFSMENIKSEFQSLQSKLATLRTPQEFFNFKKISKPQNFGEVQSRVAYNLKYFSSNYGLIIGCLSIYTLLTNLLLLFVIVLVVAGIVGINKLKGEELVTPFGSFKTNQLYTGLVCVAVPIGFLASPISTLLWLIGASAVSVFGHASLMEKPIETVFDEETV C88401CB23197CD8 ypt11_yeast GTP-binding protein YPT11 YPT11 YNL304W N0410 Rab GTPase YPT11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQRKRYSLNVVTSPSIPSPTPSAPIRTNESNWEAASPASAASSFLPNVHHGGTVLNPGLGIMRSPSLNKSGAFGRSGSSGSSTVIEPSNIKLLLIGDANVGKTAMILSYCRELLTRAEMSRSARLRHQQQQQHKDLGLKKTVVNHRLSMKEKRKRYSSNDFEKEFKDINHFADETSDFGNPNIGDDNNHEMADPNEIVIETRSTIGIDIKTNLVNIDNRFFNVILWDTAGQERYQNAIIPSLYKKTNAVILTYDITNAKSFQSCMERWIVQALENFSSQDLLKARFFLVGNKIDLYKERQVTHYDVVQMVQEMQLKHGIKISGNFEVSCKWVNVVERTMNMIILDLVENGCFENNDPCVSITTSDDVQGHEQEFHDTVEEPFNFTRQRQHQLEKNNTVDITKPNDDIANNQSICCV ypt52_yeast GTP-binding protein YPT52 YPT52 YK112 YKR014C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLQFKLVLLGDSSVGKSSIVHRFVKDTFDELRESTIGAAFLSQSITIHPNDGNETKDVVIKFEIWDTAGQERYKSLAPMYYRNANAALVVYDITQEDSLQKARNWVDELKNKVGDDDLVIYLLGNKVDLCQETPSTETSPDSNEGGDEEQKVRAISTEEAKQYAQEQGLLFREVSAKTGEGVKEIFQDIGEKLYDLKKDEILSKQNRQIGGGNNGQVDINLQRPSTNDPTSCCS 34AB2FFE38697261 ypt53_yeast GTP-binding protein YPT53 YPT53 N2223 YNL093W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDKHTAAIPTLTIKVVLLGESAVGKSSIVLRFVSDDFKESKEPTIGAAFLTKRITRDGKVIKFEIWDTAGQERFAPLAPMYYRNAQAALVVFDVTNEGSFYKAQNWVEELHEKVGHDIVIALVGNKMDLLNNDDENENRAMKAPAVQNLCERENLLYFEASAKTGENIYQIFQTLGEKVPCPEQNTRQSSTHDRTITDNQRIDLESTTVESTRETGGCNC ABA1D926D1EE9F68 ypt1_yeast GTP-binding protein YPT1 YFL038C YPT1 YP2 Rab GTPase YPT1 Transport GTPase YPT1 Protein YP2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNSEYDYLFKLLLIGNSGVGKSCLLLRFSDDTYTNDYISTIGVDFKIKTVELDGKTVKLQIWDTAGQERFRTITSSYYRGSHGIIIVYDVTDQESFNGVKMWLQEIDRYATSTVLKLLVGNKCDLKDKRVVEYDVAKEFADANKMPFLETSALDSTNVEDAFLTMARQIKESMSQQNLNETTQKKEDKGNVNLKGQSLTNTGGGCC F8C704F6BF2D227B vps21_yeast Vacuolar protein sorting-associated protein 21 GTP-binding protein YPT51 Vacuolar protein-targeting protein 12 VPS21 YOR089C YOR3154C VPS12 VPT12 YPT21 YPT51 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNTSVTSIKLVLLGEAAVGKSSIVLRFVSNDFAENKEPTIGAAFLTQRVTINEHTVKFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSFIKARHWVKELHEQASKDIIIALVGNKIDMLQEGGERKVAREEGEKLAEEKGLLFFETSAKTGENVNDVFLGIGEKIPLKTAEEQNSASNERESNNQRVDLNAANDGTSANSACSC 34D7DA0090C33F09 sec4_yeast Ras-related protein SEC4 SEC4 SRO6 Suppressor of RHO3 protein 6 YFL005W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGLRTVSASSGNGKSYDSIMKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKKVKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHANDEAQLLLVGNKSDMETRVVTADQGEALAKELGIPFIESSAKNDDNVNEIFFTLAKLIQEKIDSNKLVGVGNGKEGNISINSGSGNSSKSNCC 7DEDF7DCC7533BA7 ypt31_yeast GTP-binding protein YPT31/YPT8 Rab GTPase YPT31 YPT31 YPT8 YER031C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSEDYGYDYDLLFKIVLIGDSGVGKSNLLSRFTKNEFNMDSKSTIGVEFATRTLEIDGKRIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLSELRENADDNVAVGLIGNKSDLAHLRAVPTEESKTFAQENQLLFTETSALNSENVDKAFEELINTIYQKVSKHQMDLGDSSANGNANGASAPNGPTISLTPTPNENKKANGNNCC EC274E5873138D78 ypt32_yeast GTP-binding protein YPT32/YPT11 Rab GTPase YPT32 YPT32 YPT11 YGL210W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNEDYGYDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKSTIGVEFATRTIEVENKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLTELRENADDNVAVGLIGNKSDLAHLRAVPTDEAKNFAMENQMLFTETSALNSDNVDKAFRELIVAIFQMVSKHQVDLSGSGTNNMGSNGAPKGPTISLTPAPKEDKKKKSSNCC 621217A296161964 ypt10_yeast GTP-binding protein YPT10 YPT10 YBR1733 YBR264C protein protein yeast Saccharomyces cerevisiae Baker's yeast MEATIKVVLLGDSSVGKTSIVTRLKSGKFLAKHAATIGAAFITKTIEVPSNDSSTEKRIHMEIWDTAGQERYKSLVPMYYRDANIALIVFELGDVSSLQCAKTWFQDLQDRAQGTQVIIVGNKYDLVCEEHSGEVTIPAELQGLPYVAVSAKTGYNFDTLNKIIISLVPESQFKTLSKNNEQGNILEINKKKSGSGCIC EE66719A8C65AA54 rab1a_canlf Ras-related protein Rab-1A RAB1A RAB1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC rab5a_human Ras-related protein Rab-5A RAB5A RAB5 protein protein human Homo sapiens Human MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN gdi1_yeast Rab GDP-dissociation inhibitor YER136W GDI1 SEC19 Secretory pathway GDP dissociation inhibitor protein protein yeast Saccharomyces cerevisiae Baker's yeast MDQETIDTDYDVIVLGTGITECILSGLLSVDGKKVLHIDKQDHYGGEAASVTLSQLYEKFKQNPISKEERESKFGKDRDWNVDLIPKFLMANGELTNILIHTDVTRYVDFKQVSGSYVFKQGKIYKVPANEIEAISSPLMGIFEKRRMKKFLEWISSYKEDDLSTHQGLDLDKNTMDEVYYKFGLGNSTKEFIGHAMALWTNDDYLQQPARPSFERILLYCQSVARYGKSPYLYPMYGLGELPQGFARLSAIYGGTYMLDTPIDEVLYKKDTGKFEGVKTKLGTFKAPLVIADPTYFPEKCKSTGQRVIRAICILNHPVPNTSNADSLQIIIPQSQLGRKSDIYVAIVSDAHNVCSKGHYLAIISTIIETDKPHIELEPAFKLLGPIEEKFMGIAELFEPREDGSKDNIYLSRSYDASSHFESMTDDVKDIYFRVTGHPLVLKQRQEQEKQ 48CF8D45FC031772 ypt7_yeast Ypt/Rab-type GTPase YPT7 YPT7 VAM4 Vacuolar morphology protein 4 YM8270.02 YML001W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSRKKNILKVIILGDSGVGKTSLMHRYVNDKYSQQYKATIGADFLTKEVTVDGDKVATMQVWDTAGQERFQSLGVAFYRGADCCVLVYDVTNASSFENIKSWRDEFLVHANVNSPETFPFVILGNKIDAEESKKIVSEKSAQELAKSLGDIPLFLTSAKNAINVDTAFEEIARSALQQNQADTEAFEDDYNDAINIRLDGENNSCSC 0E2B72BDA7F7CEE5 yip1_yeast Protein transport protein YIP1 YIP1 YGR172C YPT-interacting protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFYNTSNNANNGGGFYQPSAQFAVPQGSMSFQNTVGSSNTGNDNNLGVAPDPLPVGILHALSTKGYPHEPPLLEEIGINFDHIITKTKMVLIPIRFGSGVPQEILNDSDLAGPLIFFLLFGLFLLMAGKVHFGYIYGVALFGTISLHNLSKLMSNNDTSTQTNLQFFNTASILGYCFLPLCFLSLLGIFHGLNNTTGYVVSVLFVIWSTWTSSGFLNSLLQLQNARLLIAYPLLIFYSVFALMVIFV 64372B4823A0BC5E yip3-ypt6 757365 N/A 757367 unspecified role unspecified role bait bait N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 yip3-ypt11 757365 N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 757369 unspecified role unspecified role bait bait physical association physical association false false false Table 2 yip3-ypt52 757365 N/A 757370 unspecified role unspecified role bait bait N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 yip3-ypt53 757365 N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 757371 unspecified role unspecified role bait bait physical association physical association false false false Table 2 yip3-ypt1 757365 N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 757372 unspecified role unspecified role bait bait physical association physical association false false false Table 2 yip3-ypt51 757365 N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 757373 unspecified role unspecified role bait bait physical association physical association false false false Table 2 yip3-sec4 757365 N/A 757374 unspecified role unspecified role bait bait N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 yip3-ypt31 757365 N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 757375 unspecified role unspecified role bait bait physical association physical association false false false Table 2 yip3-ypt32 757365 N/A 757376 unspecified role unspecified role bait bait N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 yip3-ypt10 757365 N/A 757377 unspecified role unspecified role bait bait N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 yip3-rab1 757365 N/A 757378 unspecified role unspecified role bait bait N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 yip3-rab5 757365 N/A 757379 unspecified role unspecified role bait bait N/A 757368 unspecified role unspecified role prey prey c-terminus exon2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 2 gdi1-rab5 757365 N/A 757379 unspecified role unspecified role bait bait N/A 757380 unspecified role unspecified role prey prey physical association physical association false false false Table 2 gdi1-rab1 757365 N/A 757378 unspecified role unspecified role bait bait N/A 757380 unspecified role unspecified role prey prey physical association physical association false false false Table 2 gdi1-ypt10 757365 N/A 757377 unspecified role unspecified role bait bait N/A 757380 unspecified role unspecified role prey prey physical association physical association false false false Table 2 gdi1-ypt32 757365 N/A 757376 unspecified role unspecified role bait bait N/A 757380 unspecified role unspecified role prey prey physical association physical association false false false Table 2 gdi1-ypt31 757365 N/A 757375 unspecified role unspecified role bait bait N/A 757380 unspecified role unspecified role prey prey physical association physical association false false false Table 2 gdi1-sec4 757365 N/A 757380 unspecified role unspecified role prey prey N/A 757374 unspecified role unspecified role bait bait physical association physical association false false false Table 2 gdi1-ypt51 757365 N/A 757380 unspecified role unspecified role prey prey N/A 757373 unspecified role unspecified role bait bait physical association physical association false false false Table 2 gdi1-ypt1-2 757365 N/A 757380 unspecified role unspecified role prey prey N/A 757372 unspecified role unspecified role bait bait physical association physical association false false false Table 2 gdi1-ypt53 757365 N/A 757380 unspecified role unspecified role prey prey N/A 757371 unspecified role unspecified role bait bait physical association physical association false false false Table 2 gdi1-ypt52 757365 N/A 757380 unspecified role unspecified role prey prey N/A 757370 unspecified role unspecified role bait bait physical association physical association false false false Table 2 gdi1-ypt11 757365 N/A 757380 unspecified role unspecified role prey prey N/A 757369 unspecified role unspecified role bait bait physical association physical association false false false Table 2 The interaction is very weak, beta-gal activity was detected after 5h. gdi1-ypt7 757365 N/A 757381 unspecified role unspecified role bait bait N/A 757380 unspecified role unspecified role prey prey physical association physical association false false false Table 2 The interaction is very weak, beta-gal activity was detected after 5h. gdi1-ypt6 757365 N/A 757380 unspecified role unspecified role prey prey N/A 757367 unspecified role unspecified role bait bait physical association physical association false false false Table 2 yip3-ypt10-1 757366 N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757377 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A yip3-ypt11-1 757366 N/A 757369 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A yip3-ypt31-1 757366 N/A 757375 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A yip3-ypt32-1 757366 N/A 757376 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A yip3-ypt52-1 757366 N/A 757370 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A yip3-ypt6-1 757366 N/A 757367 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A yip3-sec4-1 757366 N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757374 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A yip3-ypt1-1 757366 N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757372 unspecified role unspecified role prey prey n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A and 1C The C-terminus is required for the interaction to occur. yip3-yip1 757366 N/A 757368 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 757382 unspecified role unspecified role prey prey n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 3