UniProt UniProt http://www.uniprot.org lim-2001a-4 Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins. rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Synapse - Interactions of proteins with an established role in the presynapse. kime@mail.kaist.ac.kr rat brain cDNA library Mol. Cell. Neurosci. (1044-7431) 2001 lim-2001a-3 Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Synapse - Interactions of proteins with an established role in the presynapse. kime@mail.kaist.ac.kr rat brain cDNA library Mol. Cell. Neurosci. (1044-7431) 2001 lim-2001a-6 Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins. rat-brain Rattus norvegicus brain brain Brain [cerebrum] confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Synapse - Interactions of proteins with an established role in the presynapse. Hippocampal neuronal cultures prepared from E18 rats have been used. kime@mail.kaist.ac.kr rat brain cDNA library Mol. Cell. Neurosci. (1044-7431) 2001 lim-2001a-7 Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Synapse - Interactions of proteins with an established role in the presynapse. kime@mail.kaist.ac.kr rat brain cDNA library Mol. Cell. Neurosci. (1044-7431) 2001 lim-2001a-1 Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Synapse - Interactions of proteins with an established role in the presynapse. kime@mail.kaist.ac.kr rat brain cDNA library Mol. Cell. Neurosci. (1044-7431) 2001 lim-2001a-2 Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Synapse - Interactions of proteins with an established role in the presynapse. kime@mail.kaist.ac.kr rat brain cDNA library Mol. Cell. Neurosci. (1044-7431) 2001 lim-2001a-5 Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins. rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Lim S., Sala C., Yoon J., Park S., Kuroda S., Sheng M., Kim E. Synapse - Interactions of proteins with an established role in the presynapse. Hippocampal neuronal cultures prepared from E18 rats have been used. kime@mail.kaist.ac.kr rat brain cDNA library Mol. Cell. Neurosci. (1044-7431) 2001 shan1_rat SH3 and multiple ankyrin repeat domains protein 1 Shank1 GKAP/SAPAP-interacting protein Synamon Somatostatin receptor-interacting protein SPANK-1 protein protein rat Rattus norvegicus (Rat) Rat MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRGLTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTALHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARRRGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSASSPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRLFRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKASQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDGPSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALGPQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHSPHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGPEPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSPRGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPAAPPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPGPPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGGPVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGSSTAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRLSEDSQTSLLSKPSSSIFQNWPKPPLPPLPTGSGVSSSTAAAPGATSPSASSASASTRHLQGVEFEMRPPLLRRAPSPSLLPASDHKVSPAPRPSSLPILPSGPIYPGLFDIRSSPTGGAGGSTDPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLSEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL grip1_rat Glutamate receptor-interacting protein 1 Grip1 AMPA receptor-interacting protein GRIP1 protein protein rat Rattus norvegicus (Rat) Rat MIAVSFKCRCQILRRLTKDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGTTLGLTVSGGIDKDGKPRVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPVSIQGSSVMFRTVEVTLHKEGNTFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGIRLLGTTHAEAMSILKQCGQEATLLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIVIDKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHVKIQRSDRQLPWDPWASSQCSVHTNHHHNPHHPDHCRVPALGFPKALTPNSPPAMVSSSSPTSMSAYSLSSLNMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGSVFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQLLRDSSITSKVTLEIEFDVAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIRLDSCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPLGITISGTEEPFDPIIISSLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKKQTDAQPASSPKKLPIPSHSSDLGDGEEDPSPIQRPGKLSDVYPSTVPSVDSAVDSWDGSGIDARYGSQGTTFQTSGYNFNTYDWRSPKKRASLSPVPKPRSQTYPDVGLSNEDWDRSTASGFAGASDSADAEQEENFWSQALEDLETCGQSGILRELEATIMSGSTMSLNHEAPTARSQLGRQASFQERSNSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIMSPTPVELHKVTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDLGGLKPYDRLLQVNHVRTRDFDCCLVVPLIAESGNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGGNLETREPTNTL shrpn_rat Sharpin Sharpin Shank-associated RH domain-interacting protein protein protein rat Rattus norvegicus (Rat) Rat MSPPAGGAAAAADPASPVVLLAVQAAVRLLGAGHEDEAQLRKLQLKADPERPGRFRLGLLGIEPGAVSLEWPLESICYTIRGPNQHELQPPPGGPGTFSVHFLNSEEAQQWAALVRDATAEGQNGNDSTAPVPTPAMCPTSPPCSSVTPTPKATQPEMDLPQGSGNLKKEELATHLAQAIAGGDEKAAAQVAAILAQHHVALNVQLLEAWFPRGPIRLQVTVEDATSVLSSSSSAHVSLQIHPHCSIAALQEQVFSEFGFPPAVQRWVIGRCLCMPERSLASYGVSQDGDPAFLYLLSAPREVSGHSPQHSKMDRKLGCLFPQSLELPHNLQASSSSLPSPPQPGWSCPSCTFINASNRPGCEMCSTQRPCAWDPLTATST dlgp1_rat Disks large-associated protein 1 Dlgap1 Gkap Guanylate kinase-associated protein SAP90/PSD-95-associated protein 1 PSD-95/SAP90-binding protein 1 protein protein rat Rattus norvegicus (Rat) Rat MKGLSGSRSHHHGITCESACDSLSHHSDHKPYLLSPVDHHPADHPYYTQRNSFQAECVGPFSDPLASSTFPRRHYTSQQELKDESALVPRTLATKANRLPTNLLDQFERQLPLSRDGYHTLQYKRTAVEHRSDSPGRIRHLVHSVQKLFTKSHSLEGASKGGVNGGKASPDGSQTVRYGKRSKSKERRSEPKARSNASNASPTSPSWWSSDDNLDGDMCLYHTPSGVMTMGRCPDRSVSQYFMGAYNTISEQAVKASRSNNDVKCSTCANLPVTLDAPLLKKSAWSSTLTVSRAREVYQKASVNMDQAVVKSEACQQERSCQYLQVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKAMGDEDSGDSDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSLDSLDPAGLLTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMRSHSYVRAIEKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTGVIKLSSAVEVSSCITTYKKTPPPVPPRTTTKPFISITAQSSTESAQDAYMDGQGQRGDMISQSGLSNSTESLDSMKALTAAIEAANAQIHGPASQHMGSNAAAVTTTTTIATVTTEDRKKDFKKNRCLSIGIQVDDAEESEKMAESKTSSKFQSVGVQVEEEKCFRRFTRSNSVTTAVQADLDFHDNLENSLESIEDNSCPGPMARQFSRDASTSTVSIQGSGNHYHACAADDDFDTDFDPSILPPPDPWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDRMEGWCKQMEREERENNLPEDILGKIRTAVGSAQLLMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDMLQLSIENISMKFDELHQLKANNWKQMDPLDKKERRAPPPVPKKPAKGPAPLIRERSLESSQRQEARKRLMAAKRAASVRQNSATESAESIEIYIPEAQTRL shank1-dlg4-1 666122 N/A 666129 unspecified role unspecified role bait bait N/A 666130 unspecified role unspecified role prey prey physical association physical association false false false 5B shank1-grip1-1 666122 N/A 666131 unspecified role unspecified role prey prey N/A 666129 unspecified role unspecified role bait bait physical association physical association false false false 5B sharpin-dlg4-1 666122 N/A 666132 unspecified role unspecified role bait bait N/A 666130 unspecified role unspecified role prey prey physical association physical association false false false 5B sharpin-shank1-3 666122 N/A 666132 unspecified role unspecified role bait bait N/A 666129 unspecified role unspecified role prey prey physical association physical association false false false 5B sharpin-grip1-1 666122 N/A 666132 unspecified role unspecified role bait bait N/A 666131 unspecified role unspecified role prey prey physical association physical association false false false 5B dlgap1-shank1-1 666122 N/A 666129 unspecified role unspecified role prey prey N/A 666133 unspecified role unspecified role bait bait physical association physical association false false false 5B dlgap1-dlg4-3 666122 N/A 666133 unspecified role unspecified role bait bait N/A 666130 unspecified role unspecified role prey prey physical association physical association false false false 5B dlgap1-grip1-1 666122 N/A 666131 unspecified role unspecified role prey prey N/A 666133 unspecified role unspecified role bait bait physical association physical association false false false 5B dlg4-shank1-4 666122 N/A 666129 unspecified role unspecified role prey prey N/A 666130 unspecified role unspecified role bait bait physical association physical association false false false 5B dlg4-grip1-1 666122 N/A 666130 unspecified role unspecified role bait bait N/A 666131 unspecified role unspecified role prey prey physical association physical association false false false 5B shank1-sharpin-3 666123 N/A 666132 unspecified role unspecified role prey prey chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 666129 unspecified role unspecified role bait bait chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false 5a sharpin-shank1-2 666123 N/A 666132 unspecified role unspecified role bait bait chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 666129 unspecified role unspecified role prey prey chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false 5a shank1-sharpin-7 666124 N/A 666132 unspecified role unspecified role neutral component neutral component N/A 666129 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 8 Used anti-Shank antibody recognizes Shank1m Shank2 and Shank3. sharpin-dlg4-2 666124 N/A 666132 unspecified role unspecified role neutral component neutral component N/A 666130 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 8 sharpin-sharpin-1 666125 N/A 666132 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 666132 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false 8 sharpin-sharpin-2 666125 N/A 666132 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 666132 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false 8 shank1-sharpin-1 666126 N/A 666132 unspecified role unspecified role prey prey n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 666129 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false no figure shank1-sharpin-2 666127 N/A 666132 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 666129 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false 2 shank1-sharpin-6 666128 N/A 666132 unspecified role unspecified role neutral component neutral component N/A 666129 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6 Used anti-Shank antibody recognizes Shank1m Shank2 and Shank3. Co-localization occurs in punctate patternm within dendrites.