IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ croze-2000-1 Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Croze E., Usacheva A., Asarnow D., Minshall RD., Perez HD., Colamonici O. Apoptosis - Interactions involving proteins with a function related to apoptosis ed_croze@berlex.com Human B-cell J. Immunol. (0022-1767) 2000 Only protein-protein interactions imex curation Synapse - Interactions of proteins with an established role in the presynapse. croze-2000-2 Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Croze E., Usacheva A., Asarnow D., Minshall RD., Perez HD., Colamonici O. Apoptosis - Interactions involving proteins with a function related to apoptosis ed_croze@berlex.com Human B-cell J. Immunol. (0022-1767) 2000 Only protein-protein interactions imex curation Synapse - Interactions of proteins with an established role in the presynapse. croze-2000-3 Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor. human-daudi Homo sapiens Burkitt's B-cell lymphona cells daudi Homo sapies DAUDI Burkitt's B-cell lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Croze E., Usacheva A., Asarnow D., Minshall RD., Perez HD., Colamonici O. Apoptosis - Interactions involving proteins with a function related to apoptosis ed_croze@berlex.com Human B-cell J. Immunol. (0022-1767) 2000 Only protein-protein interactions imex curation Synapse - Interactions of proteins with an established role in the presynapse. croze-2000-4 Receptor for activated C-kinase (RACK-1), a WD motif-containing protein, specifically associates with the human type I IFN receptor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Croze E., Usacheva A., Asarnow D., Minshall RD., Perez HD., Colamonici O. Apoptosis - Interactions involving proteins with a function related to apoptosis Directed two-hybrid. ed_croze@berlex.com Human B-cell J. Immunol. (0022-1767) 2000 Only protein-protein interactions imex curation Synapse - Interactions of proteins with an established role in the presynapse. rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR inar2_human Interferon alpha/beta receptor 2 IFNAR2 IFNARB Type I interferon receptor 2 IFNABR Interferon alpha binding protein protein protein human Homo sapiens Human MLLSQNAFIFRSLNLVLMVYISLVFGISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAKIGGIITVFLIALVLTSTIVTLKWIGYICLRNSLPKVLNFHNFLAWPFPNLPPLEAMDMVEVIYINRKKKVWDYNYDDESDSDTEAAPRTSGGGYTMHGLTVRPLGQASATSTESQLIDPESEEEPDLPEVDVELPTMPKDSPQQLELLSGPCERRKSPLQDPFPEEDYSSTEGSGGRITFNVDLNSVFLRVLDDEDSDDLEAPLMLSSHLEEMVDPEDPDNVQSNHLLASGEGTQPTFPSPSSEGLWSEDAPSDQSDTSESDVDLGDGYIMR ifnar2-gnb2l1-1 Interaction of IFNAR2 and GNB2L1 identified by Y2H 491572 N/A 491576 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false N/A 491577 unspecified role unspecified role bait bait cytoplasmic region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 491582 491580 physical association physical association false false false 1 ifnar2-gnb2l1-2 Interaction of IFNAR2 and GNB2L1 identified by GST pulldown 491573 N/A 491576 unspecified role unspecified role prey prey human-lymphoblast Homo sapiens, lymphoblast lymphoblast Transformed lymphocytes; Blast cell; Lymphoblastoid N/A 491577 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 2 ifnar2-gnb2l1-3 Interaction of IFNAR2 and GNB2L1 identified by coimmunoprecipitation 491574 N/A 491577 unspecified role unspecified role bait bait N/A 491576 unspecified role unspecified role prey prey physical association physical association false false false 3 ifnar2-gnb2l1-4 Interaction of IFNAR2 and GNB2L1 identified by Y2H 491575 N/A 491576 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 491577 unspecified role unspecified role bait bait cytoplasmic region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B