IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fenster-2000-5 Piccolo, a presynaptic zinc finger protein structurally related to bassoon. rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] confocal microscopy confocal microscopy immunostaining immunostaining Fenster SD., Chung WJ., Zhai R., Cases-Langhoff C., Voss B., Garner AM., Kaempf U., Kindler S., Gundelfinger ED., Garner CC. Synapse - Interactions of proteins with an established role in the presynapse. The Bait assumed to be of rat in origin since this is the only Piccolo cDNA discussed by the authors. The authors have however, not specifically mentioned the origin of the protien used. Hippocampal neurons cultured for 24 days were studied. Synaptotagmin and GABAA are distributed over multiple uniprot entries. These were also shown to colocalize with Piccolo protein. garner@nrc.uab.edu Mouse brain MATCHMAKER LexA cDNA library Neuron (0896-6273) 2000 fenster-2000-2 Piccolo, a presynaptic zinc finger protein structurally related to bassoon. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fenster SD., Chung WJ., Zhai R., Cases-Langhoff C., Voss B., Garner AM., Kaempf U., Kindler S., Gundelfinger ED., Garner CC. Synapse - Interactions of proteins with an established role in the presynapse. The Bait assumed to be of rat in origin since this is the only Piccolo cDNA discussed by the authors. The authors have however, not specifically mentioned the origin of the protien used. Hippocampal neurons cultured for 24 days were studied. Synaptotagmin and GABAA are distributed over multiple uniprot entries. These were also shown to colocalize with Piccolo protein. garner@nrc.uab.edu Mouse brain MATCHMAKER LexA cDNA library Neuron (0896-6273) 2000 fenster-2000-1 Piccolo, a presynaptic zinc finger protein structurally related to bassoon. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Fenster SD., Chung WJ., Zhai R., Cases-Langhoff C., Voss B., Garner AM., Kaempf U., Kindler S., Gundelfinger ED., Garner CC. Synapse - Interactions of proteins with an established role in the presynapse. The Bait assumed to be of rat in origin since this is the only Piccolo cDNA discussed by the authors. The authors have however, not specifically mentioned the origin of the protien used. Hippocampal neurons cultured for 24 days were studied. Synaptotagmin and GABAA are distributed over multiple uniprot entries. These were also shown to colocalize with Piccolo protein. garner@nrc.uab.edu Mouse brain MATCHMAKER LexA cDNA library Neuron (0896-6273) 2000 fenster-2000-3 Piccolo, a presynaptic zinc finger protein structurally related to bassoon. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Fenster SD., Chung WJ., Zhai R., Cases-Langhoff C., Voss B., Garner AM., Kaempf U., Kindler S., Gundelfinger ED., Garner CC. Synapse - Interactions of proteins with an established role in the presynapse. The Bait assumed to be of rat in origin since this is the only Piccolo cDNA discussed by the authors. The authors have however, not specifically mentioned the origin of the protien used. Hippocampal neurons cultured for 24 days were studied. Synaptotagmin and GABAA are distributed over multiple uniprot entries. These were also shown to colocalize with Piccolo protein. garner@nrc.uab.edu Mouse brain MATCHMAKER LexA cDNA library Neuron (0896-6273) 2000 fenster-2000-4 Piccolo, a presynaptic zinc finger protein structurally related to bassoon. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Fenster SD., Chung WJ., Zhai R., Cases-Langhoff C., Voss B., Garner AM., Kaempf U., Kindler S., Gundelfinger ED., Garner CC. Synapse - Interactions of proteins with an established role in the presynapse. The Bait assumed to be of rat in origin since this is the only Piccolo cDNA discussed by the authors. The authors have however, not specifically mentioned the origin of the protien used. Hippocampal neurons cultured for 24 days were studied. Synaptotagmin and GABAA are distributed over multiple uniprot entries. These were also shown to colocalize with Piccolo protein. garner@nrc.uab.edu Mouse brain MATCHMAKER LexA cDNA library Neuron (0896-6273) 2000 fenster-2000-6 Piccolo, a presynaptic zinc finger protein structurally related to bassoon. rat-brain Rattus norvegicus brain brain Brain [cerebrum] density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Fenster SD., Chung WJ., Zhai R., Cases-Langhoff C., Voss B., Garner AM., Kaempf U., Kindler S., Gundelfinger ED., Garner CC. Synapse - Interactions of proteins with an established role in the presynapse. The Bait assumed to be of rat in origin since this is the only Piccolo cDNA discussed by the authors. The authors have however, not specifically mentioned the origin of the protien used. Hippocampal neurons cultured for 24 days were studied. Synaptotagmin and GABAA are distributed over multiple uniprot entries. These were also shown to colocalize with Piccolo protein. garner@nrc.uab.edu Mouse brain MATCHMAKER LexA cDNA library Neuron (0896-6273) 2000 fenster-2000-7 Piccolo, a presynaptic zinc finger protein structurally related to bassoon. rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fenster SD., Chung WJ., Zhai R., Cases-Langhoff C., Voss B., Garner AM., Kaempf U., Kindler S., Gundelfinger ED., Garner CC. Synapse - Interactions of proteins with an established role in the presynapse. The Bait assumed to be of rat in origin since this is the only Piccolo cDNA discussed by the authors. The authors have however, not specifically mentioned the origin of the protien used. Hippocampal neurons cultured for 24 days were studied. Synaptotagmin and GABAA are distributed over multiple uniprot entries. These were also shown to colocalize with Piccolo protein. garner@nrc.uab.edu Mouse brain MATCHMAKER LexA cDNA library Neuron (0896-6273) 2000 bsn_rat Protein bassoon Bsn protein protein rat Rattus norvegicus (Rat) Rat MGNEASLEGGAGEGPLPPGGSGLGPGPGAGKPPSALAGGGQLPVAGAARAAGPPTPGLGLVPGPGPGPGPGSVSRRLDPKEPLGSQRATSPTPKQASATAPGRESPRETRAQGLSGQEAEGPRRTLQVDSRTQRSGRSPSVSPDRGSTPTSPYSVPQIAPLPSSTLCPICKTSDLTSTSSQPNFNTCTQCHNKVCNQCGFNPNPHLTQVKEWLCLNCQMQRALGMDMTTAPRSKSQQQLHSPALSPAHSPAKQPLGKPEQERSRSPGATQSGPRQAEAARATSVPGPTQATAPPEVGRVSPQPPLSTKPSTAEPRPPAGEAQGKSATTVPSGLGAAEQTQGGLTGKLFGLGASLLTQASTLMSVQPEADTQGQPSPSKGPPKIVFSDASKEAGPRPPGSGPGPGPTPGAKTEPGPRTGPGSGPGALAKTGGTPSPKHGRADHQAASKAAAKPKTMPKERAACPLCQAELNVGSRGPANYNTCTACKLRVCTLCGFNPTPHLVEKTEWLCLNCQTKRLLEGSLGEPAPLPLPTPQEPPAGVPQRAAGASPLKQKGPQGPGQPSGSLPPKASPQAAKASPQAAKASPQAKPLRASEPSKTSSSAPEKKTGIPVKAEPVPKPPPETAVPPGTPKAKSGVKRTDPATPVVKPVPEAPKSGEAEEPVPKPYSQDLSRSPQSLSDTGYSSDGVSSSQSEITGVVQQEVEQLDSAGVTGPRPPSPSELHKVGSSMRPSLEAQAVAPSGEWSKPPSGSAVEDQKRRPHSLSIMPEAFDSDEELGDILEEDDSLAMGRQREQQDTAESSDDFGSQLRHDYVEDSSEGGLSPLPPQPPARADMTDEEFMRRQILEMSAEEDNLEEDDTAVSGRGLAKHGAQKASARPRPESSQESVALPKRRLPHNATTGYEELLSEEGPAEPTDGALQGGLRRFKTIGLNSTGRLWSTSLDLGQGSDPNLDREPELEMESLTGSPEDRSRGEHSSTLPASTPSYTSGTSPTSLSSLEEDSDSSPSRRQRLEEAKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLREQEKMREVEQQRIRSTARKTRRDKEELRAQRRRERSKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSPSLDSEAETLDGGPTRLYKSGSEYNLPAFMSLCSPTETPSGSSTTPSSGRPLKSAEEAYEDMMRKAELLQRQQGQAAGARGPHGGPSQPTGPRSQGSFEYQDTLDHDYGGRASQPAADGTPAGLGATVYEEILQTSQSIARMRQASSRDLAFTEDKKKEKQFLNAESAYMDPMKQNGGPLTPGTSPTQLAAPVSFPTSTSSDSSGGRVIPDVRVTQHFAKEPQEPLKLHSSPASPSLASKEVGMTFSQGPGTPATTAMAPCPASLPRGYMTPAGPERSPSTSSTIHSYGQPPTTANYGSQTEELPHAPSGPAGSGRASREKPLSGGDGEVGPPQPSRGYSYFTGSSPPLSPSTPSESPTFSPSKLGPRATAEFSTQTPSLTPSSDIPRSVGTPSPMVAQGTQTPHRPSTPRLVWQQSSQEAPVMVITLASDASSQTRMVHASASTSPLCSPTDSQPASHSYSQTTPPSASQMPSEPAGPPGFPRAPSAGVDGPLALYGWGALPAENISLCRISSVPGTSRVEPGPRPPGTAVVDLRTAVKPTPIILTDQGMDLTSLAVEARKYGLALDPVPGRQSTAVQPLVINLNAQEQTHTFLATATTVSITMASSVLMAQQKQPVVYGDPFQSRLDFGQGSGSPVCLAQVKQVEQAVQTAPYRGGPRGRPREAKFARYNLPNQVTPLARRDILITQMGTAQSVSLKPGPVPEPGAEPHRATPAELRAHALPGTRKPHTVVVQMGEGAAGTVTTLLPEEPAGALDLTGMRPESRLACCDMAYKFPFGSSCTGTFHPAPSAPDKSVTDAALPGQSSGPFYSPRDPEPPEPLTFRAQGVVGPGPHEEQRPYPQGLPGRLYSSMSDTNLAEAGLNYHAQRIGQLFQGPGRDSAVDLSSLKHSYSLGFADGRYLGQGLQYGSFTDLRHPTDLLSHPLPMRPYSSVSNIYSDHRYGPRGDAVGFQEASLAQYSATTAREISRMCAALNSMDQYGGRHGGGSGGPDLVPYQPQHGPGLNAPQGLASLRSGLLGNPTYPEGQPSPGNLAQYGPAASQGTAVRQLLPSTATVRAADGMIYSTINTPIAATLPITTQPASVLRPMVRGGMYRPYGSGGVTAVPLTSLTRVPMIAPRVPLGPAGLYRYPAPSRFPIASTIPPAEGPVYLGKPAAAKASGAGGPPRPELPAGGAREEPLSTTAPPAVIKEAPVAQAPAPPPGQKPAGDAAAGSGSGVLGRPVMEKEEASQEDRQRKQQEQLLQLERERVELEKLRQLRLQEELERERVELQRHREEEQLLVQRELQELQTIKHHVLQQQQEERQAQFALQREQLAQQRLQLEQIQQLQQQLQQQLEEQKQRQKAPFPATCEAPSRGPPPAATELAQNGQYWPPLTHTAFIAVAGTEGPGQAREPVLHRGLPSSASDMSLQTEEQWEAGRSGIKKRHSMPRLRDACEPESGPDPSTVRRIADSSVQTDDEEGEGRYLLTRRRRTRRSADCSVQTDDEDNAEWEQPVRRRRSRLSRHSDSGSDSKHEASASSSAAAAAARAMSSVGIQTISDCSVQTEPEQLPRVSPAIHITAATDPKVEIVRYISAPEKTGRGESLACQTEPDGQAQGVAGPQLIGPTAISPYLPGIQIVTPGALGRFEKKKPDPLEIGYQAHLPPESLSQLVSRQPPKSPQVLYSPVSPLSPHRLLDTSFASSERLNKAHVSPQKQFIADSTLRQQTLPRPMKTLQRSLSDPKPLSPTAEESAKERFSLYQHQGGLGSQVSALPPNGLVRKVKRTLPSPPPEEAHLPLAGQVPSQLYAASLLQRGLAGPTTVPATKASLLRELDRDLRLVEHESTKLRKKQAELDEEEKEIDAKLKYLELGITQRKESLAKDRVGRDYPPLRGLGEHRDYLSDSELNQLRLQGCTTPAGQYVDYPASAAVPATPSGPTAFQQPRFPPAATQYTAGSSGPTQNGFLAHQAPTYTGPSTYPAPTYPPGTSYPAEPGLPSQPAFHPTGHYAAPTPMPTTQSAPFPVQADSHAAHQKPRQTSLADLEQKVPTNYEVISSPAVTVSSTPSETGYSGPAVSSSYEHGKAPEHPRGGDRSSVSQSPAPTYPSDSHYTSLEQNVPRNYVMIDDISELTKDSTPTASDSQRPEPLGPGGVSGRPGKDPGEPAVLEGPTLPCCYGRGEEESEEDSYDPRGKSGHHRSMESNGRPASTHYYSDSDYRHGARADKYGPGPMGPKHPSKNLAPAAISSKRSKHRKQGMEQKISKFSPIEEAKDVESDLASYPPPTVSSSLTSRSRKFQDEITYGLKKNVYEQQRYYGVSSRDTAEEDDRMYGGSSRSRVASAYSGEKLSSHDFSSRSKGYERERETAQRLQKAGPKPSSLSMAHGRARPPMRSQASEEESPVSPLGRPRPAGGALPPGDTCPQFCSSHSMPDVQEHVKDGPRAHAYKREEGYILDDSHCVVSDSEAYHLGQEETDWFDKPRDARSDRFRHHGGHTVSSSQKRGPARHSYHDYDEPPEEGLWPHDEGGPGRHTSAKEHRHHGDHGRHSGRHAGEEPGRRAARPHARDMGRHETRPHPQASPAPAMQKKGQPGYPSSADYSQPSRAPSAYHHASDSKKGSRQAHSGPTVLQPKPEAQAQPQMQGRQAVPGPQQSQPPSSRQTPSGTASRQPQTQQQQQQQQQQQQQQQQQQQQQQQQGLGQQAPQQAPSQARLQQQSQPTTRSTAPAASHPAGKPQPGPTTAPGPQPAGLPRAEQAGSSKPAAKAPQQGRAPQAQSAPGPAGAKTGARPGGTPGAPAGQPAAEGESVFSKILPGGAAEQAGKLTEAVSAFGKKFSSFW pclo_rat Protein piccolo Pclo Aczonin Multidomain presynaptic cytomatrix protein protein protein rat Rattus norvegicus (Rat) Rat MGNEASLEGEGLPEGLAAAAGAGGSGSALHPGIPAGMEADLSQLSEEERRQIAAVMSRAQGLPKGSVPPAAAESPSMHRKQELDSSQAPQQPGKPPDPGRPTQPGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDFKEEYKSSMMPGFFSDVNPLSAVSSVVNKFNPFDLISDSEASQEETTKKQKVVQKEQGKSEGMAKPPLQQPSPKPIPKQQGQVKEVIQQDSSPKSVSSQQAEKVKPQAPGTGKPSQQSPAQTPAQQASPGKPVAQQPGSAKATVQQPGPAKSPAQPAGTGKSPAQPPAKTPGQQAGLEKTSSSQQPGPKSLAQTPGHGKFPLGPVKSPAQQPGTAKHPAQQPGPQTAAKVPGPTKTPAQQSGPGKTPAQQPGPTKPSPQQPIPAKPQPQQPVATKTQPQQSAPAKPQPQQPAPAKPQPQQPTPAKPQPQPPTPAKPQPQPPTATKPQPQPPTATKPHHQQPGLAKPSAQQPTKSISQTVTGRPLQPPPTSAAQTPAQGLSKTICPLCNTTELLLHIPEKANFNTCTECQSTVCSLCGFNPNPHLTEIKEWLCLNCQMQRALGGDLAAAIPSSPQPTPKAATAPTATASKSPVPSQQASPKKEPPSKQDSPKALESKKPPEPKKPPEPKKPPEPKKPPPLVKQPTLHGPTPATAPQLPVAEALPEPAPPKEPSGPLPEQAKAPVGDVEPKQPKMTETRADIQSSSTTKPDILSSQVQSQAQVKTASPLKTDSAKPSQSFPPTGEKTTPLDSKAMPRPASDSKIISQPGPGSESKDPKHIDPIQKKDEPKKAQPKGSPKPETKPVPKGSPTPSGTRPTAGQAAPPSQQPPKPQEQSRRFSLNLGGITDAPKSQPTTPQETVTGKLFGFGASIFSQASNLISTAGQQGPHPQTGPAAPSKQAPTPSQSPAAQGPAKSTGQLPPAPAKATAVKKEAKAAAAENLESKPEQAPTAKKTEKDKKPPPAKVGKPPPSEPEKAVPAHKPDKTTKPKPACPLCRTELNLGSQEPPNFNTCTECKNQVCNLCGFNPTPHLTEIQEWLCLNCQTQRAISGQLGDMGKMPPAPSGPKASPMPAPAEPSSQKTPTGTQVKGKKKEAEGKTEAEKPVPEKETASIEKTPPMVTTDQKLEESEGKKSKVSALPEKKPSEEEKAISADKKERKPPAEEKPPLEEKKPIPVDKKLPPEAKPLSSEGEEKHEILKAHVQIPEEEPTGKVAAKAGEEEQQPDSRPEALPGATPLTLPKAGEKERAVAQPQAEGSSKDGQGERSKEKTEKEEDKSDTSSSQQPKSPQGLSDTGYSSDGISGSLGEIPSLIPSDEKDLLKGLKKDSFSQESSPSSPSDLAKLESTVLSILEAQASTLVGEKAEKKTQPQKISPEKPQDQQKTQTASETLDITISEEEIKESQEKKVSPKKDSEQGFPSRKEHKEKPELVDDLSPRRASYDSVEDSSESENSPVVRRKRRTSIGSSSSDEYKQEDSQGSGEEEDFIRKQIIEMSADEDASGSEDEEFIRSQLKEISGVGESQKREEAKGKGKGVAGKHRRLTRKSSTSFDDDAGRRHSWHDEDDETFDESPELKFRETKSQESEELVVAGGGGLRRFKTIELNSTIADKYSSESSQKKTILYFDEEPELEMESLTDSPEDRSRGEGSSSLHASSFTPGTSPTSVSSLDEDSDSSPSHKKGESKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLKEQEKQRELEQQQRKSSSKKSKKDKDELRAQRRRERPKTPPSNLSPIEDASPTEELRQAAEMEELHRSSCSEYSPSIESDPEGFEISPEKIIEVQKVYKLPAAVSLYSPTDEQSVMQKEGVQKALKSAEEMYEEMMQKPHKYKAFPAANERDEVFEKEPLYGGMLIEDYIYESLVEDTYNGSVDGSLLTRQEEQNGFMQQRGREQKVRLQEQIYDDPMQKISDLQKEFYELESLHSVVPQEDIVSSSYIIPESHEIVDLGSMVMSTSEEKKLLDADSAYEELMRRQQVQVTDGSSPVQTTIGDDMAESTLDFDRVQDASLTSSILSGASLTDSTSSATLSIPDVKITQQFSAEELEDEYVTDYTREIQDIIAHESLILTYSEPSESATSVPPSDTPSLTSSISSVCTTDSSSPVTTLDSLTTVYTEPADVMTKFKDSEEISSTYFPGSIIDYPEDISVSLDRTIMPESRTNEDRIVLSFSGMAPSVVESVGTKPERPQADTISTDLPISEKDLIKGKKETGDGIILEVLDAYKDKREESEAELTKISLPEPGLAQAPSSVTAPQIKEQHVSPHSVSGKISGQEKPTYRLPSGSLPVSTHPSKSRPFFRSSSLDISAQPPPPPPPPPPSPSTSSPPPTPPLPPATSPKPPTYPKKKLAVAATVTSTTIVTTHVDALTMVEAAAARRSNGLPATKMCAIAPPPVPPKPSQIPTGLVFTHRPEAIKPPIAPKPAVPQIPVTTQKPTDTCPKPTGLSLTSTMSLNLVTSADYNVPSPTSPLSPHSNKSSPRYSKSLMDTYVVITLPSEPGTPTDSSAAQAITSWPLGSPPKDLVSLETVFSVVPPMTSTEIPSASQPTLYTSGALGTFSVTPAVTASLFQTVPTSLTQFLPAEASKPEVSAVSSAVPSVAPRSVSIPIPPEPLALDRHQYKENGKLPLIGDAIDLRTIPKSEVKVTEKCMDLSASAMDVKRQTTANEVYRRQISAVQPSIINLSAASSLGTPVTMDSKTVAVVTCTDTTIYTTGTESQVGIEHAVTSPLQLTTSKHTELPYRKPSSQAFPTIRDEAPINLSLGPSAQAVTLAVTKPVTVPPVGVTNGWTDSTLSQGVADGEVVDLSTSKSHRTVVTMDESTSNVVTKIIEDDEKPVDLTAGRRAVCCDMVYTLPFGRSCTAQQPATTLPEDRFGYRDDHYQYDRSGPYGYRGIGGMKPSMSDTNLPEAGHFFYKSKNAFDYSGGTGAAVDLTSGRVSTGEVMDYSSKTTGPYPETRQVISGVGISTPQYSTARLTPPPGPQYGVGSVLRSSNGVVYSSVATPIPSTFAITTQPGSIFSTTVRDLSGIPTTDAMTSLSALHQSQPMPRSYFITTGASETDIAVTGIDINASLQTITMETLPAETMDSVPTLTTASEVFSEVVGEESTLLIVPDEDKQQQQLDLERELLELEKIKQQRFAEELEWERQEIQRFREQEKIMVQKKLEELQSMKQHLLYQQEEERQAQFMMRQETLAQQQLQLEQIQQLQQQLHQQLEEQKLRQIYQYNYDPSGTSSPQTTTEQAILEGQYAATEGSQFWATEDATTTASTVVAIEIPQSQGWYTVQSDGVTQYIAPPGILSTVSEIPLTDVVVKEEKQPKKRSSGAKVRGQYDEMGESVADDPRNLKKIVDSGVQTDDEETADRSYASRRRRTKKSVDTSVQTDDEDQDEWDMPSRSRRKARTGKYGDSTAEGDKTKPLSKVSSVAVQTVAEISVQTEPVGTIRTPSIRARVDAKVEIIKHISAPEKTYKGGSLGCQTETDSDTQSPPYLGATSPPKDKKRPTPLEIGYSSSHLRADPTVQLAPSPPKSPKVLYSPISPLSPGNALEPAFVPYEKPLPDDISPQKVLHPDMAKVPPASPKTAKMMQRSMSDPKPLSPTADESSRAPFQYSEGFTTKGSQTMTASGTQKKVKRTLPNPPPEEVSTGTQSTYSTMGTASRRRMCRTNTMARAKILQDIDRELDLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVAEDRDYMSDSEVSSTRPSRVESQHGVERPRTAPQTEFSQFIPPQTQTEAQLVPPTSPYTQYQYSSPALPTQAPTPYTQQSHFQQQTLYHQQVSPYQTQPTFQAVATMSFTPQAQPTPTPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSNYEVIRNQPLMIAPVSTDNTYAVSHLGSKYNSLDLRIGLEERSSMAGSPISSISADSFYADIDHHTSRNYVLIDDIGDITKGTAALSTVFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQTSSRLHSYVKADEDPMEDPYELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAAKQLPAAILYQKQSKHKKSLIDPKMSKFSPIQESRDLEPDYPTYMSSGTSSIGGISSRARLLQDDITFGLRKNITDQQKFMGSSLGSGLGTLGNTIRSALQDEADKPYSSGSRSRPSSRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFGHSSSSARTKPTSLPISQSRGRIPIVAQSSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREESRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPERLVHSRPLSQHQEQILQMNGKTIHYIFPHARVKITRDFKDHTGSGNGLGIRIVGGKEIPGHSGEIGAYIAKILPGESAEHTGPLMEGMQVLEWNGVPLTSKTYEEVQSIINQQSGEAEICVRLDLNMLSDSENPQHLELHEPPKVDKAKSPGVDPKQLAAELQKVSLQQSPLVMSSVVEKGSHAHSGPTSAGSSSVPSPGQPGSPSVSKKKHSSTKPTDGPKAASHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASAEYKRRTKYVQKSLNPEWNQTVIYKSISMEQLMKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTSHLDNTPRWYPLKEQTESIDHGKSHSSQNSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVAQTHLEDAGVAIAAAEAAVQQLRIQPTKPTNHRPAESSVSTGSSGSSVGSGYSVDSEGSSCVAGEPNLLPIPRIGKMGQNGQDPVKQPGMGATDTEGKTQVMGEIKLALKKEMKTDGEQLIVEILQCRNITYKFKSPDHLPDLYVKLYVINISTQKKVIKKKTRVCRHDREPSFNETFRFSLSPAGHSLQILLFSNGGKFMKKTLIGEACIWLDKVDLRKRIVNWHKLLVSPTQTH gria1_rat Glutamate receptor 1 Gria1 GluR-A GluR-K1 Glutamate receptor ionotropic, AMPA 1 AMPA-selective glutamate receptor 1 Glur1 protein protein rat Rattus norvegicus (Rat) Rat MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDSRDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGGGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL praf1_rat Prenylated Rab acceptor protein 1 Rabac1 Pra1 Praf1 PRA1 family protein 1 protein protein rat Rattus norvegicus (Rat) Rat MAAQKDQQKDAEVEGLSATTLLPKLIPSGAGREWLERRRATIRPWGTFVDQQRFSRPRNVGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLQSKLVLFGREVSPAHQYALAGGVSFPFFWLAGAGSAVFWVLGATLVLIGSHAAFHQIEPADGEELQMEPV praf1_mouse Prenylated Rab acceptor protein 1 PRA1 family protein 1 Prenylin Rabac1 Pra Pra1 Praf1 protein protein mouse Mus musculus Mouse MAAQKDQQKDAEGEGLSATTLLPKLIPSGAGREWLERRRATIRPWGTFVDQQRFSRPRNVGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLQSKLVLFGREVSPAHQYALAGGVSFPFFWLAGAGSAVFWVLGATLVLIGSHAAFHQMEPADGEELQMEPV rp3a_mouse Rabphilin-3A Rph3a Exophilin-1 protein protein mouse Mus musculus Mouse MTDTVVNRWMYPGDGPLQSNDKEQLQAGWSVHPGAQTDRQRKQEELTDEEKEIINRVIARAEKMEAMEQERIGRLVDRLETMRKNVAGDGVNRCILCGEQLGMLGSACVVCEDCKKNVCTKCGVETSNNRPHPVWLCKICLEQREVWKRSGAWFFKGFPKQVLPQPMPIKKTKPQQPAGEPATQEQPTPESRHPARAPARGDMEDRRPPGQKPGPDLTSAPGRGSHGPPTRRASEARMSTAARDSEGWDHAHGGGTGDTSRSPAGLRRANSVQAARPAPAPVPSPAPPQPVQPGPPGGSRATPGPGRFPEQSTEAPPSDPGYPGAVAPAREERTGPAGGFQAAPHTAAPYSQAAPARQPPPAEEEEEEANSYDSDEATTLGALEFSLLYDQDNSNLQCTIIRAKGLKPMDSNGLADPYVKLHLLPGASKSNKLRTKTLRNTRNPVWNETLQYHGITEEDMQRKTLRISVCDEDKFGHNEFIGETRFSLKKLKANQRKNFNICLERVIPMKRAGTTGSARGMALYEEEQVERIGDIEERGKILVSLMYSTQQGGLIVGIIRCVHLAAMDANGYSDPFVKLWLKPDMGKKAKHKTQIKKKTLNPEFNEEFFYDIKHSDLAKKSLDISVWDYDIGKSNDYIGGCQLGISAKGERLKHWYECLKNKDKKIERWHQLQNENHVSSD rab3a_mouse Ras-related protein Rab-3A Rab3a protein protein mouse Mus musculus Mouse MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLTDQQAPPHQDCAC praf1_human Prenylated Rab acceptor protein 1 PRA1 family protein 1 RABAC1 PRA1 PRAF1 protein protein human Homo sapiens Human MAAQKDQQKDAEAEGLSGTTLLPKLIPSGAGREWLERRRATIRPWSTFVDQQRFSRPRNLGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLESKLVLFGREVSPAHQYALAGGISFPFFWLAGAGSAVFWVLGATLVVIGSHAAFHQIEAVDGEELQMEPV syph_rat Synaptophysin Major synaptic vesicle protein p38 Syp protein protein rat Rattus norvegicus (Rat) Rat MDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYTGELRLSVECANKTESALNIEVEFEYPFRLHQVYFDAPSCVKGGTTKIFLVGDYSSSAEFFVTVAVFAFLYSMGALATYIFLQNKYRENNKGPMMDFLATAVFAFMWLVSSSAWAKGLSDVKMATDPENIIKEMPMCRQTGNTCKELRDPVTSGLNTSVVFGFLNLVLWVGNLWFVFKETGWAAPFMRAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPASGGGGYGPQGDYGQQGYGQQGAPTSFSNQM rab3a_rat Ras-related protein Rab-3A Rab3a protein protein rat Rattus norvegicus (Rat) Rat MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLTDQQAPPHQDCAC vamp2_rat Vesicle-associated membrane protein 2 Vamp2 Syb2 Synaptobrevin-2 protein protein rat Rattus norvegicus (Rat) Rat MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST pic-bsn 771074 n/a 771081 unspecified role unspecified role neutral component neutral component n/a 771082 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4D, 4E, 4F pic-glur 771074 n/a 771083 unspecified role unspecified role neutral component neutral component n/a 771082 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4G, 4H, 4I pic-pra1 771074 n/a 771084 unspecified role unspecified role neutral component neutral component n/a 771082 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6A pclo-rabac1-1 771075 n/a 771085 unspecified role unspecified role prey prey n/a 771082 unspecified role unspecified role bait bait region zn2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5A rab3a-rabp 771075 n/a 771086 unspecified role unspecified role bait bait n terminal optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false n/a 771087 unspecified role unspecified role prey prey physical association physical association false false false 5A pclo-rabac1 771076 n/a 771085 unspecified role unspecified role prey prey n/a 771082 unspecified role unspecified role bait bait region zn2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false pclo-rabac1-2 771077 n/a 771085 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 771082 unspecified role unspecified role prey prey zn1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false zn2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 771088 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 5B pclo-rabac1-3 771078 n/a 771085 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 771082 unspecified role unspecified role bait bait zn finger 2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false zn finger 1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 5D Triton X-100 pra1-syp 771079 n/a 771089 unspecified role unspecified role neutral component neutral component n/a 771084 unspecified role unspecified role neutral component neutral component association association false false false 6C pra1-syn 771080 n/a 771090 unspecified role unspecified role prey prey n/a 771084 unspecified role unspecified role prey prey n/a 771091 unspecified role unspecified role prey prey n/a 771089 unspecified role unspecified role bait bait acetylation acetylation reaction acetylation false false false 6D In the bound fraction, PRA1 was affinity purified along with synaptophysin, rab3A and VAMP/Synaptobrevin II ( Figure 6D).