UniProt UniProt http://www.uniprot.org chin-2000-1 SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 chin-2000-2 SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. in vitro In vitro pull down pull down antibody detection identification by antibody antibody detection Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 chin-2000-3 SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 chin-2000-4 SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. PC12 cells have been treated with NGF to induce formation of neurites. lianli@med.unc.edu rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 srcn1_rat SRC kinase signaling inhibitor 1 Srcin1 Snip p140Cap SNAP-25-interacting protein P140 p130Cas-associated protein protein protein rat Rattus norvegicus (Rat) Rat MQPWQCLRRFALAWWERTAEGRARSPREEVGPRDPGGRGEPDPERSSPPMLSADDAEYPREYRTLGGGGSGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPASHLASSSPPPGLPSGLPSGLPSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPTSQGVSPSPSAILERRDVKPDEDLAGKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAGDPFAYPGAGGLYKRGSVRSLSTYSAAALQSDLEDSLYKAGAGGPLYGDGYGFRLPPSSPQKLADVSAPSGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKARSTGASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKIEGSNGAATPSAPVCGSGSRSSGATPVSGPPPPAVSSTPAGQPTAVSRLQMQMHLRGLQNSASDLRGQLQQLRKLQLQNQESVRALLKRTEAELSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVAHNHRLVPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRVVTDTLAQIRRQVDEGVWPPPNNLLNQSPKKVAAETDFSKGLDFEIPPPSPPLNLHELSGPAEGTPLTPKSGNPTKGLDAPSKRNMDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKTHPGPTPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSVFIKKAESEELEIQKPQVKLRRAVSEVVRPASTPPIMASAIKDEDDEERIIAELESGGGSVPPMKVVTPGASRLKAAQGPAGSPDKGKHGKQRTEYMRIQAQQQVRVGYQAPRPLEGCTPRLCYMPLS p60879-1 Synaptosomal-associated protein 25 Snap25 Snap Super protein Synaptosomal-associated 25 kDa protein SNAP-25b protein protein mouse Mus musculus Mouse MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG snp25_rat Synaptosomal-associated protein 25 Snap25 Snap Synaptosomal-associated 25 kDa protein Super protein protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG snap25-snip-1 665886 N/A 665890 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 665891 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false no figure snip-snap25-1 665887 N/A 665890 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 665892 unspecified role unspecified role prey prey physical association physical association false false false 2A snap25-snip-2 665888 N/A 665890 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 665892 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false 2B snap25-snip-3 665889 N/A 665890 unspecified role unspecified role neutral component neutral component N/A 665892 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6 Co-localization occurs in filopodia, lamellipodia, neutitic processes and periphery of cell soma. Partial co-localization with actin cytoskeleton was shown using labeled F-actin.