IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hwang-2014-6 Tumor suppressor PDCD4 inhibits NF-κB-dependent transcription in human glioblastoma cells by direct interaction with p65. human-u251 Homo sapiens u251 U2521 glioblastoma cell line ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Hwang S.K., Baker A.R., Young M.R., Colburn N.H. IBD - Inflammatory bowel disease Human glioblastoma-derived cell lines U251 and LN229 were stably transfected with human PDCD4 by transfecting a PDCD4/pFB-blast retroviral vector and Blasticidin S selection. The origin of the HA-tagged human p65 expression constructs was derived from PMID:15192014 and PMID:12559944. police042@naver.com Carcinogenesis (0143-3334) 2014 Only protein-protein interactions imex curation hwang-2014-1 Tumor suppressor PDCD4 inhibits NF-κB-dependent transcription in human glioblastoma cells by direct interaction with p65. human-u251 Homo sapiens u251 U2521 glioblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hwang S.K., Baker A.R., Young M.R., Colburn N.H. IBD - Inflammatory bowel disease Human glioblastoma-derived cell lines U251 and LN229 were stably transfected with human PDCD4 by transfecting a PDCD4/pFB-blast retroviral vector and Blasticidin S selection. The origin of the HA-tagged human p65 expression constructs was derived from PMID:15192014 and PMID:12559944. police042@naver.com Carcinogenesis (0143-3334) 2014 Only protein-protein interactions imex curation hwang-2014-2 Tumor suppressor PDCD4 inhibits NF-κB-dependent transcription in human glioblastoma cells by direct interaction with p65. human-ln_229 Homo sapiens malignant glioma cell line LN-229 ln_229 Human malignant glioma cell line LN-229 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hwang S.K., Baker A.R., Young M.R., Colburn N.H. IBD - Inflammatory bowel disease Human glioblastoma-derived cell lines U251 and LN229 were stably transfected with human PDCD4 by transfecting a PDCD4/pFB-blast retroviral vector and Blasticidin S selection. The origin of the HA-tagged human p65 expression constructs was derived from PMID:15192014 and PMID:12559944. police042@naver.com Carcinogenesis (0143-3334) 2014 Only protein-protein interactions imex curation hwang-2014-3 Tumor suppressor PDCD4 inhibits NF-κB-dependent transcription in human glioblastoma cells by direct interaction with p65. human-u251 Homo sapiens u251 U2521 glioblastoma cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hwang S.K., Baker A.R., Young M.R., Colburn N.H. IBD - Inflammatory bowel disease Human glioblastoma-derived cell lines U251 and LN229 were stably transfected with human PDCD4 by transfecting a PDCD4/pFB-blast retroviral vector and Blasticidin S selection. The origin of the HA-tagged human p65 expression constructs was derived from PMID:15192014 and PMID:12559944. police042@naver.com Carcinogenesis (0143-3334) 2014 Only protein-protein interactions imex curation hwang-2014-4 Tumor suppressor PDCD4 inhibits NF-κB-dependent transcription in human glioblastoma cells by direct interaction with p65. human-ln_229 Homo sapiens malignant glioma cell line LN-229 ln_229 Human malignant glioma cell line LN-229 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hwang S.K., Baker A.R., Young M.R., Colburn N.H. IBD - Inflammatory bowel disease Human glioblastoma-derived cell lines U251 and LN229 were stably transfected with human PDCD4 by transfecting a PDCD4/pFB-blast retroviral vector and Blasticidin S selection. The origin of the HA-tagged human p65 expression constructs was derived from PMID:15192014 and PMID:12559944. police042@naver.com Carcinogenesis (0143-3334) 2014 Only protein-protein interactions imex curation hwang-2014-5 Tumor suppressor PDCD4 inhibits NF-κB-dependent transcription in human glioblastoma cells by direct interaction with p65. human-u251 Homo sapiens u251 U2521 glioblastoma cell line confocal microscopy confocal microscopy immunostaining immunostaining Hwang S.K., Baker A.R., Young M.R., Colburn N.H. IBD - Inflammatory bowel disease Human glioblastoma-derived cell lines U251 and LN229 were stably transfected with human PDCD4 by transfecting a PDCD4/pFB-blast retroviral vector and Blasticidin S selection. The origin of the HA-tagged human p65 expression constructs was derived from PMID:15192014 and PMID:12559944. police042@naver.com Carcinogenesis (0143-3334) 2014 Only protein-protein interactions imex curation birc2_human_gene Baculoviral IAP repeat-containing protein 2 human gene gene gene human Homo sapiens Human tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS ccnd1_human_gene Human Cyclin D1 gene gene gene human Homo sapiens Human vegfa_human_gene Human VEGFA gene gene gene human Homo sapiens Human mmp9_human_gene Human MMP9 gene;Matrix metalloproteinase-9 gene gene human Homo sapiens Human pdcd4_human Programmed cell death protein 4 PDCD4 H731 Nuclear antigen H731-like Neoplastic transformation inhibitor protein Protein 197/15a protein protein human Homo sapiens Human MDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKRRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFHHELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY if4a1_human Eukaryotic initiation factor 4A-I EIF4A1 EIF4A DDX2A ATP-dependent RNA helicase eIF4A-1 protein protein human Homo sapiens Human MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI rela-birc2-1 1763622 n/a 1763628 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1763629 unspecified role unspecified role bait bait association association false false false Fig. 5g PDCD4 (Q53EL6) The interaction was not observed in U251 cells stably overexpressing human PDCD4. Q53EL6 decreases binding of Q04206 to ENSG00000110330 rela-ccnd1 1763622 n/a 1763630 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1763629 unspecified role unspecified role bait bait association association false false false Fig. 5g PDCD4 (Q53EL6) The interaction was not observed in U251 cells stably overexpressing human PDCD4. Q53EL6 decreases binding of Q04206 to ENSG00000110092 rela-vegfa 1763622 n/a 1763631 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1763629 unspecified role unspecified role bait bait association association false false false Fig. 5g PDCD4 (Q53EL6) The interaction was not observed in U251 cells stably overexpressing human PDCD4. Q53EL6 decreases binding of Q04206 to ENSG00000112715 rela-mmp9 1763622 n/a 1763629 unspecified role unspecified role bait bait n/a 1763632 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 5g PDCD4 (Q53EL6) The interaction was not observed in U251 cells stably overexpressing human PDCD4. Q53EL6 decreases binding of Q04206 to ENSG00000100985 pdcd4-rela 1763623 n/a 1763629 unspecified role unspecified role prey prey naturally occurring naturally occurring endogenous endogenous n/a 1763633 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 4a, 4e pdcd4-eif4a1 1763623 n/a 1763633 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q53EL6:p.Asp418Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q53EL6:p.[Asp253Ala;Asp418Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1763634 unspecified role unspecified role prey prey naturally occurring naturally occurring endogenous endogenous physical association physical association false false false Fig. 4h pdcd4-eif4a1-1 1763623 n/a 1763629 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey nucl delivery nucleic acid delivery nucl delivery n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1763633 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n/a 1763634 unspecified role unspecified role prey prey naturally occurring naturally occurring endogenous endogenous association association false false false Fig. 4h The asp-418ala mutation, alone or in combination with asp253ala mutation, in PDCD4 decreased binding to eIF4A but did not affect binding to p65. pdcd4-rela-1 1763624 n/a 1763629 unspecified role unspecified role prey prey naturally occurring naturally occurring endogenous endogenous n/a 1763633 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 4b, 4f rela-pdcd4 1763625 n/a 1763633 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n/a 1763629 unspecified role unspecified role bait bait nucl delivery nucleic acid delivery nucl delivery n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 4c, 4i rela-pdcd4-1 1763626 n/a 1763629 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1763633 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Fig. 4d pdcd4-rela-2 1763627 n/a 1763633 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n/a 1763629 unspecified role unspecified role neutral component neutral component naturally occurring naturally occurring endogenous endogenous colocalization colocalization false false false Fig. 4g