IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ liu-2012h-1 The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Liu S., Li L., Zhang Y., Zhang Y., Zhao Y., You X., Lin Z., Zhang X., Ye L. IBD - Inflammatory bowel disease The identity of HBXIP was inferred from previous work of the authors (PMID:21343296). zhlin720@ybu.edu.cn, zhangxd@nankai.edu.cn, yelihong@nankai.edu.cn J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation liu-2012h-2 The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu S., Li L., Zhang Y., Zhang Y., Zhao Y., You X., Lin Z., Zhang X., Ye L. IBD - Inflammatory bowel disease The identity of HBXIP was inferred from previous work of the authors (PMID:21343296). zhlin720@ybu.edu.cn, zhangxd@nankai.edu.cn, yelihong@nankai.edu.cn J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation liu-2012h-3 The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa autoradiography autoradiography Liu S., Li L., Zhang Y., Zhang Y., Zhao Y., You X., Lin Z., Zhang X., Ye L. IBD - Inflammatory bowel disease The identity of HBXIP was inferred from previous work of the authors (PMID:21343296). zhlin720@ybu.edu.cn, zhangxd@nankai.edu.cn, yelihong@nankai.edu.cn J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation stat4_human Signal transducer and activator of transcription 4 STAT4 protein protein human Homo sapiens Human MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE s100a4_human_gene Human S100 calcium binding protein A4 gene gene human Homo sapiens Human ltor5_human Ragulator complex protein LAMTOR5 LAMTOR5 HBXIP Hepatitis B virus X-interacting protein Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5 XIP protein protein human Homo sapiens Human MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAAKLTSDPTDIPVVCLESDNGNIMIQKHDGITVAVHKMAS s10a4_human_core-s1-1_dna-probe Human S10A4 gene promoter core regulation region S1-1 DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human GTGCTAAAGAGATCCAGATGTGAGATTCTGATGTGGAACT stat4-s100a4 1597801 n/a 1597804 unspecified role unspecified role bait bait n/a 1597805 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 3c Q14765 increases expression at protein level of ENSG00000196154 Q14765 increases expression at RNA level of ENSG00000196154 lamtor5-s100a4 1597801 n/a 1597806 unspecified role unspecified role bait bait n/a 1597805 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 2b O43504 increases expression at RNA level of ENSG00000196154 O43504 increases expression at protein level of ENSG00000196154 lamtor5-stat4 1597802 n/a 1597806 unspecified role unspecified role bait bait n/a 1597804 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3d stat4-lamtor5 1597802 n/a 1597806 unspecified role unspecified role prey prey n/a 1597804 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3d s10a4-lamtor5 1597803 n/a 1597806 unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells n/a 1597807 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 2f, 3b In contrast to HBXIP/LTOR5 present in nuclear cell extracts, purified HBXIP/LTOR5 could not bind to the S1-1 S100A4 promoter DNA probe. s10a4-stat4 1597803 n/a 1597804 unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells n/a 1597807 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 3b