IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chaudhry-2009-2 mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Chaudhry A., Rudra D., Treuting P., Samstein RM., Liang Y., Kas A., Rudensky AY. IBD - Inflammatory bowel disease wild-type Tregs rudenska@mskcc.org Science (New York, N.Y.) 2009 Only protein-protein interactions imex curation imex curation chaudhry-2009-1 mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chaudhry A., Rudra D., Treuting P., Samstein RM., Liang Y., Kas A., Rudensky AY. IBD - Inflammatory bowel disease Nuclear lysates of sorted CD4+Foxp3− and CD4+Foxp3+ cells obtained from lymph nodes, spleen and Peyer’s patches. In Supp. figure 1 cells were stimulated with IL-2, IL-6 and IL-10 +/- inhibitors. For Figure 1 no interleukins are stated to have been added. rudenska@mskcc.org Science (New York, N.Y.) 2009 Only protein-protein interactions imex curation imex curation il6_mouse_gene Interleukin-6 Il6 Il-6 gene gene mouse Mus musculus Mouse foxp3_mouse Forkhead box protein P3 Foxp3 Scurfin protein protein mouse Mus musculus Mouse MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKRSQRPNKCSNPCP stat3_mouse Signal transducer and activator of transcription 3 Acute-phase response factor Stat3 Aprf protein protein mouse Mus musculus Mouse MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM tgfb1_mouse_gene Transforming growth factor beta-1 Tgfb1 gene gene mouse Mus musculus Mouse il2ra_mouse_gene Interleukin-2 receptor subunit alpha gene gene gene mouse Mus musculus Mouse ikzf2_mouse_gene Zinc finger protein Helios Ikzf2 Helios Zfpn1a2 Znfn1a2 gene gene mouse Mus musculus Mouse foxp3-il6-1 1884195 N/A 1884197 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1884198 unspecified role unspecified role bait bait association association false false false 5G P42227 is required for the binding of Q99JB6 to ENSMUSG00000025746 P42227 increases the binding of Q99JB6 to ENSMUSG00000025746 stat3-il6-1 1884195 N/A 1884197 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1884199 unspecified role unspecified role bait bait association association false false false 5G-2 P42227 decreases expression at RNA level of ENSMUSG00000025746 P42227 decreases expression at protein level of ENSMUSG00000025746 stat3-tgfb1-1 1884195 N/A 1884200 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1884199 unspecified role unspecified role bait bait association association false false false 5G-3 P42227 decreases expression at RNA level of ENSMUSG00000002603 P42227 decreases expression at protein level of ENSMUSG00000002603 foxp3-tgfb1-1 1884195 N/A 1884200 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1884198 unspecified role unspecified role bait bait association association false false false 5G-4 P42227 increases the binding of Q99JB6 to ENSMUSG00000002603 P42227 is required for the binding of Q99JB6 to ENSMUSG00000002603 foxp3-il2ra_mouse_gene-4 1884195 N/A 1884201 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1884198 unspecified role unspecified role bait bait association association false false false 5G-5 P42227 is not required for the binding of Q99JB6 to ENSMUSG00000026770 foxp3-ikzf2-1 1884195 N/A 1884202 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1884198 unspecified role unspecified role bait bait association association false false false 5G-6 P42227 is not required for the binding of Q99JB6 to ENSMUSG00000026770 foxp3-stat3-1 1884196 N/A 1884199 unspecified role unspecified role prey prey tyr705 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false N/A 1884198 unspecified role unspecified role bait bait physical association physical association false false false 1A, Supp 1 CHEMBL1923234 (WP1066 - a Stat3 inhibitor) A Stat3 inhibitor described in Supp fig 1 as "Inh Peptide" may be CHEBI:90286 - (Stat3 inhibitor peptide)