IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lee-2009-8 mouse-t-cell_th2 mouse helper T-lymphocyte Th2 Mouse t-cell_th2 T-helper cell 2 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lee CG., Kang KH., So JS., Kwon HK., Son JS., Song MK., Sahoo A., Yi HJ., Hwang KC., Matsuyama T., Yui K., Im SH. IBD - Inflammatory bowel disease ChIP is stated to have been carried out using NFAT2 antibodies, but no results are shown in Figure 2E. The text also states:-"NFAT1 binding to the reported IL-4 and IFN-gamma promoter regions are shown as positive controls for the NFAT1 ChIP in Th1 and Th2 cells, respectively." However Fig 2E shows binding with NFAT1 in Th1 cells for the IFN-gamma promoter and the IL4 promoter in Th2 cells. Cells were purified from the lymph nodes and spleen. Cells were stimulated with PMA and ionomycin. imsh@gist.ac.kr Molecular immunology 2009 Only protein-protein interactions imex curation imex curation lee-2009-6 in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Lee CG., Kang KH., So JS., Kwon HK., Son JS., Song MK., Sahoo A., Yi HJ., Hwang KC., Matsuyama T., Yui K., Im SH. IBD - Inflammatory bowel disease imsh@gist.ac.kr Molecular immunology 2009 Only protein-protein interactions imex curation imex curation lee-2009-7 mouse-t-cell_th1 Mouse T helper cell Th1 t-cell_th1 T-helper cell 1 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lee CG., Kang KH., So JS., Kwon HK., Son JS., Song MK., Sahoo A., Yi HJ., Hwang KC., Matsuyama T., Yui K., Im SH. IBD - Inflammatory bowel disease ChIP is stated to have been carried out using NFAT2 antibodies, but no results are shown in Figure 2E. The text also states:-"NFAT1 binding to the reported IL-4 and IFN-gamma promoter regions are shown as positive controls for the NFAT1 ChIP in Th1 and Th2 cells, respectively." However Fig 2E shows binding with NFAT1 in Th1 cells for the IFN-gamma promoter and the IL4 promoter in Th2 cells. Cells were purified from the lymph nodes and spleen. Cells were stimulated with PMA and ionomycin. imsh@gist.ac.kr Molecular immunology 2009 Only protein-protein interactions imex curation imex curation il10_mouse_gene Interleukin-10 gene gene gene mouse Mus musculus Mouse nfac2_mouse Nuclear factor of activated T-cells, cytoplasmic 2 T-cell transcription factor NFAT1 NFAT pre-existing subunit Nfatc2 Nfat1 Nfatp protein protein mouse Mus musculus Mouse MDVPEPQPDPDGGDGPGHEPGGSPQDELDFSILFDYDYLNPIEEEPIAHKAISSPSGLAYPDDVLDYGLKPCNPLASLSGEPPGRFGEPDSIGFQNFLSPVKPAGASGPSPRIEITPSHELMQAGGALRGRDAGLSPEQPALALAGVAASPRFTLPVPGYEGYREPLCLSPASSGSSASFISDTFSPYTSPCVSPNNAGPDDLCPQFQNIPAHYSPRTSPIMSPRTSLAEDSCLGRHSPVPRPASRSSSPGAKRRHSCAEALVAPLPAASPQRSRSPSPQPSPHVALQDDSIPAGYPPTAGSAVLMDALNTLATDSPCGIPSKIWKTSPDPTPVSTAPSKAGLARHIYPTVEFLGPCEQEERRNSAPESILLVPPTWPKQLVPAIPICSIPVTASLPPLEWPLSNQSGSYELRIEVQPKPHHRAHYETEGSRGAVKAPTGGHPVVQLHGYMENKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGNTKVLEIPLEPKNNMRATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHVPEPSGRIVSLQAASNPIECSQRSAHELPMVERQDMDSCLVYGGQQMILTGQNFTAESKVVFMEKTTDGQQIWEMEATVDKDKSQPNMLFVEIPEYRNKHIRVPVKVNFYVINGKRKRSQPQHFTYHPVPAIKTEPSDEYEPSLICSPAHGGLGSQPYYPQHPMLAESPSCLVATMAPCQQFRSGLSSPDARYQQQSPAAALYQRSKSLSPGLLGYQQPSLLAAPLGLADAHRSVLVHAGSQGQGQGSTLPHTSSASQQASPVIHYSPTNQQLRGGGHQEFQHIMYCENFGPSSARPGPPPINQGQRLSPGAYPTVIQQQTAPSQRAAKNGPSDQKEALPTGVTVKQEQNLDQTYLDDVNEIIRKEFSGPPSRNQT il4_mouse_gene Interleukin-4 Il4 Il-4 gene gene mouse Mus musculus Mouse irf4_mouse Interferon regulatory factor 4 Irf4 Spip Lymphocyte-specific interferon regulatory factor NF-EM5 PU.1 interaction partner Transcriptional activator PIP protein protein mouse Mus musculus Mouse MNLETGSRGSEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSVFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLDDTQMAMGHPYPMTAPYGSLPAQQVHNYMMPPHDRSWRDYAPDQSHPEIPYQCPVTFGPRGHHWQGPSCENGCQVTGTFYACAPPESQAPGIPIEPSIRSAEALALSDCRLHICLYYRDILVKELTTTSPEGCRISHGHTYDVSNLDQVLFPYPDDNGQRKNIEKLLSHLERGLVLWMAPDGLYAKRLCQSRIYWDGPLALCSDRPNKLERDQTCKLFDTQQFLSELQVFAHHGRPAPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNTGHFLRGYELPEHVTTPDYHRSLRHSSIQE cns9c_enhancer_il10_gene_probe ds dna double stranded deoxyribonucleic acid ds DNA ds dna mouse Mus musculus Mouse ACGGCGAGAAGGAAACACAGGTGAACACGC nfac1_mouse Nuclear factor of activated T-cells, cytoplasmic 1 Nfatc1 Nfat2 Nfatc NFAT transcription complex cytosolic component protein protein mouse Mus musculus Mouse MPNTSFPVPSKFPLGPPAAVCGSGETLRPAPPSGGTMKAAEEEHYSYVSPSVTSTLPLPTAHSALPAACHDLQTSTPGISAVPSANHPPSYGGAVDSGPSGYFLSSGNTRPNGAPTLESPRIEITSYLGLHHGSGQFFHDVEVEDVLPSCKRSPSTATLHLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGFPRSLGACHLLGSPRHSPSTSPRASITEESWLGARGSRPTSPCNKRKYSLNGRQPSCSPHHSPTPSPHGSPRVSVTEDTWLGNTTQYTSSAIVAAINALTTDSTLDLGDGVPIKSRKTALEHAPSVALKVEPAGEDLGTTPPTSDFPPEEYTFQHLRKGAFCEQYLSVPQASYQWAKPKSLSPTSYMSPSLPALDWQLPSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPIVQLHGYLENEPLTLQLFIGTADDRLLRPHAFYQVHRITGKTVSTTSHEIILSNTKVLEIPLLPENNMRAIIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPNGRTLSLQVASNPIECSQRSAQELPLVEKQSTDSYPVIGGKKMVLSGHNFLQDSKVIFVEKAPDGHHVWEMEAKTDRDLCKPNSLVVEIPPFRNQRITSPAQVSFYVCNGKRKRSQYQRFTYLPANGNSVFLTLSSESELRGGFY mint Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells cns9a_enhancer_il10_gene_probe ds dna double stranded deoxyribonucleic acid ds DNA ds dna mouse Mus musculus Mouse CTCACAACGAAACTGCAACCTCAAAGCACC ifng_mouse_gene Interferon gamma Ifng gene gene mouse Mus musculus Mouse nfatc2-il10_mouse_gene-1 1876491 N/A 1876494 unspecified role unspecified role prey prey cns9_enhancer_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1876495 unspecified role unspecified role bait bait association association false false false 2E CHEBI:63954 and CHEBI:37537 increase the expression at RNA level of ENSMUSG00000016529 CHEBI:4031 decreases the expression at RNA level of ENSMUSG00000016529 Q60591 increases the expression at RNA level of ENSMUSG00000016529 nfatc2-il10_mouse_gene-2 1876491 N/A 1876495 unspecified role unspecified role bait bait N/A 1876496 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2E-2 positive control CHEBI:63954 and CHEBI:37537 increase the expression at RNA level of ENSMUSG00000000869 nfatc2-il10_mouse_gene-3 1876491 N/A 1876494 unspecified role unspecified role prey prey cns9_enhancer_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1876497 unspecified role unspecified role bait bait association association false false false 2Ei irf4-il4-1 1876491 N/A 1876497 unspecified role unspecified role bait bait N/A 1876496 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2E-2i weak interaction cns9c_enhancer_il10_gene_probe-nfatc2-2 1876492 N/A 1876495 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line N/A 1876498 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 2C cns9c_enhancer_il10_gene_probe-nfatc2-1 1876492 N/A 1876499 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line N/A 1876498 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 2C-2 CHEBI:63954 and CHEBI:37537 increase the expression at RNA level of ENSMUSG00000016529 O88942 and Q60591 increase the expression at RNA level of ENSMUSG00000016529 cns9a_enhancer_il10_gene_probe-irf4-1 1876492 N/A 1876497 unspecified role unspecified role prey prey mouse-t-cell_th2 mouse helper T-lymphocyte Th2 Mouse t-cell_th2 T-helper cell 2 N/A 1876500 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 2D nfatc2-ifng-1 1876493 N/A 1876501 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1876495 unspecified role unspecified role bait bait association association false false false 2E positive control CHEBI:63954 and CHEBI:37537 increase the expression at RNA level of ENSMUSG00000055170