IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ park-2007b-1 Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Park J., Lee J.H., La M., Jang M.J., Chae G.W., Kim S.B., Tak H., Jung Y., Byun B., Ahn J.K., Joe C.O. IBD - Inflammatory bowel disease Origin of DAXX constructs inferred from previous work of the author (PMID:15240113). Origin of the GST-fused p65 construct inferred from Fig. 3b in PMID:22249179. jkahn@cnu.ac.kr,cojoe@kaist.ac.kr J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation park-2007b-3 Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Park J., Lee J.H., La M., Jang M.J., Chae G.W., Kim S.B., Tak H., Jung Y., Byun B., Ahn J.K., Joe C.O. IBD - Inflammatory bowel disease Origin of DAXX constructs inferred from previous work of the author (PMID:15240113). Origin of the GST-fused p65 construct inferred from Fig. 3b in PMID:22249179. jkahn@cnu.ac.kr,cojoe@kaist.ac.kr J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation park-2007b-5 Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Park J., Lee J.H., La M., Jang M.J., Chae G.W., Kim S.B., Tak H., Jung Y., Byun B., Ahn J.K., Joe C.O. IBD - Inflammatory bowel disease Origin of DAXX constructs inferred from previous work of the author (PMID:15240113). Origin of the GST-fused p65 construct inferred from Fig. 3b in PMID:22249179. jkahn@cnu.ac.kr,cojoe@kaist.ac.kr J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation park-2007b-2 Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park J., Lee J.H., La M., Jang M.J., Chae G.W., Kim S.B., Tak H., Jung Y., Byun B., Ahn J.K., Joe C.O. IBD - Inflammatory bowel disease Origin of DAXX constructs inferred from previous work of the author (PMID:15240113). Origin of the GST-fused p65 construct inferred from Fig. 3b in PMID:22249179. jkahn@cnu.ac.kr,cojoe@kaist.ac.kr J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation park-2007b-4 Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Park J., Lee J.H., La M., Jang M.J., Chae G.W., Kim S.B., Tak H., Jung Y., Byun B., Ahn J.K., Joe C.O. IBD - Inflammatory bowel disease 24 hours after transfection, cells were treated with 20 ng/ml tumor necrosis factor alpha (TNFalpha) for 30 minutes before cell lysate for co-immunopreipitation was prepared. Origin of DAXX constructs inferred from previous work of the author (PMID:15240113). Origin of the GST-fused p65 construct inferred from Fig. 3b in PMID:22249179. jkahn@cnu.ac.kr,cojoe@kaist.ac.kr J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS ikba_human_gene NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 gene gene human Homo sapiens Human il8_human_gene Human IL-8 gene gene gene human Homo sapiens Human cbp_human CREB-binding protein CREBBP CBP Histone lysine acetyltransferase CREBBP Protein-lysine acetyltransferase CREBBP protein protein human Homo sapiens Human MAENLLDGPPNPKRAKLSSPGFSANDSTDFGSLFDLENDLPDELIPNGGELGLLNSGNLVPDAASKHKQLSELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQGQPNSANMASLSAMGKSPLSQGDSSAPSLPKQAASTSGPTPAASQALNPQAQKQVGLATSSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLINQASQGQAQVMNGSLGAAGRGRGAGMPYPTPAMQGASSSVLAETLTQVSPQMTGHAGLNTAQAGGMAKMGITGNTSPFGQPFSQAGGQPMGATGVNPQLASKQSMVNSLPTFPTDIKNTSVTNVPNMSQMQTSVGIVPTQAIATGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGTGQQNATSLSNPNPIDPSSMQRAYAALGLPYMNQPQTQLQPQVPGQQPAQPQTHQQMRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGSNSGNIGTLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPAPGAQPPVIPQAQPVRPPNGPLSLPVNRMQVSQGMNSFNPMSLGNVQLPQAPMGPRAASPMNHSVQMNSMGSVPGMAISPSRMPQPPNMMGAHTNNMMAQAPAQSQFLPQNQFPSSSGAMSVGMGQPPAQTGVSQGQVPGAALPNPLNMLGPQASQLPCPPVTQSPLHPTPPPASTAAGMPSLQHTTPPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGSVPSATQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEVDEKKPEVKVEVKEEEESSSNGTASQSTSPSQPRKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNRYHFCEKCFTEIQGENVTLGDDPSQPQTTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRPRKENKFSAKRLQTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAFEEIDGVDVCFFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYVTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIIHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKKEESTAASETTEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVINTLPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFSSLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHAHKMVKWGLGLDDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQSLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVSMSPAGFPSVARTQPPTTVSTGKPTSQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRVNINNSMPPGRTGMGTPGSQMAPVSLNVPRPNQVSGPVMPSMPPGQWQQAPLPQQQPMPGLPRPVISMQAQAAVAGPRMPSVQPPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYVANQPGMQPQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPQQQAMGGLNPQGQALNIMNPGHNPNMASMNPQYREMLRRQLLQQQQQQQQQQQQQQQQQQGSAGMAGGMAGHGQFQQPQGPGGYPPAMQQQQRMQQHLPLQGSSMGQMAAQMGQLGQMGQPGLGADSTPNIQQALQQRILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQIATSLSNQVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSIDQGHLGNPEQSAMLPQLNTPSRSALSSELSLVGDTTGDTLEKFVEGL rela-daxx 1574676 n/a 1574681 unspecified role unspecified role prey prey n/a 1574682 unspecified role unspecified role bait bait physical association physical association false false false Fig. 8a tumor necrosis factor alpha (TNFalpha) No Daxx was co-immunoprecipitated with p65 from HeLa cells not stimulated with tumor necrosis factor alpha (TNFalpha). daxx-rela 1574676 n/a 1574681 unspecified role unspecified role bait bait n/a 1574682 unspecified role unspecified role prey prey physical association physical association false false false Fig. 8b tumor necrosis factor alpha (TNFalpha) No p65 was co-immunoprecipitated with Daxx from HeLa cells not stimulated with tumor necrosis factor alpha (TNFalpha). daxx-rela-1 1574677 n/a 1574681 unspecified role unspecified role neutral component neutral component native native n/a 1574682 unspecified role unspecified role neutral component neutral component native native colocalization colocalization false false false Fig. 4, Fig. 8c tumor necrosis factor alpha (TNFalpha) Daxx and p65 co-localized in nuclei of HeLa cells after stimulation with tumor necrosis factor alpha (TNFalpha) (20 ng/ml) for 30 minutes. daxx-rela-2 1574677 n/a 1574681 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1574682 unspecified role unspecified role neutral component neutral component native native colocalization colocalization false false false Fig. 4 rela-nfkbia-11 1574678 n/a 1574682 unspecified role unspecified role bait bait n/a 1574683 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 1d tumor necrosis factor alpha (TNFalpha) No IKBA-specific chromatin was co-immunoprecipitated with p65 from HeLa cells not stimulated with tumor necrosis factor alpha (TNFalpha). Co-expression of FLAG-tagged Daxx decreased the amount of IKBA-specific chromatin co-precipitated with p65. Q04206 increases expression at RNA level of ENSG00000100906 Q9UER7 decreases activity of Q04206 Q9UER7 decreases expression at RNA level of ENSG00000100906 rela-il8-3 1574678 n/a 1574684 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1574682 unspecified role unspecified role bait bait association association false false false Fig. 1d tumor necrosis factor alpha (TNFalpha) No IL8-specific chromatin was co-immunoprecipitated with p65 from HeLa cells not stimulated with tumor necrosis factor alpha (TNFalpha). Co-expression of FLAG-tagged Daxx decreased the amount of IL8-specific chromatin co-precipitated with p65. Q04206 increases expression at RNA level of ENSG00000169429 Q9UER7 decreases expression at RNA level of ENSG00000169429 rela-daxx-1 1574679 n/a 1574681 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1574682 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 8d rela-crebbp 1574680 n/a 1574682 unspecified role unspecified role bait bait n/a 1574685 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 7