IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tottey-2002-1 A copper metallochaperone for photosynthesis and respiration reveals metal-specific targets, interaction with an importer, and alternative sites for copper acquisition. ecoli Escherichia coli (strain K12) transcription compl transcriptional complementation assay transcription compl predetermined predetermined participant predetermined Tottey S., Rondet SA., Borrelly GP., Robinson PJ., Rich PR., Robinson NJ. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species fig. 2 n.j.robinson@newcastle.ac.uk J. Biol. Chem. (0021-9258) 2002 Only protein-protein interactions imex curation tottey-2002-2 A copper metallochaperone for photosynthesis and respiration reveals metal-specific targets, interaction with an importer, and alternative sites for copper acquisition. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom predetermined predetermined participant predetermined Tottey S., Rondet SA., Borrelly GP., Robinson PJ., Rich PR., Robinson NJ. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species A Q-Sepharose anion exchange matrix is used to confirm the transfert of copper from copper-Atx1 to apoPacS. fig. 2 n.j.robinson@newcastle.ac.uk J. Biol. Chem. (0021-9258) 2002 Only protein-protein interactions imex curation atcs_syny3 Probable copper-transporting ATPase PacS pacS sll1920 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MAQTINLQLEGMRCAACASSIERAIAKVPGVQSCQVNFALEQAVVSYHGETTPQILTDAVERAGYHARVLKQQVLSSQQTEDRKPVFSAKLVTGLVISAVLFFGSLPMMLGVNIPHFPHIFHDPWLQWLLATPVQFWSGAEFYRGAWKSVRTRSATMDTLVALGTSAAYFYSVAITLFPQWLTSQGLAAHVYFEAAAVVITLILLGRSLEQRARRETSAAIRKLMGLQPQTALVKRGEHWETVAIAELAINDVVRVRPGEKIPVDGVVVAGNSTVDESLVTGESFPVDKTVGTEVIGATLNKSGSLDIQVSKLGQDSVLAQIIQLVQQAQASKAPIQHFVDRITHWFVPTVIVVAIAAFCIWWLTTGNITLAVLTLVEVLIIACPCALGLATPTSVMVGTGKGAEYGVLIKEASSLEMAEKLTAIVLDKTGTLTQGKPSVTNFFTLSPTSTEESLQLIQWAASVEQYSEHPLAEAVVNYGQSQQVSLLEIDNFQAIAGCGVAGQWQGQWIRLGTSNWLTDLGVTGTEHQPWQSQAQQWEKEQKTVIWLAVDTEVKALLAIADAIKPSSPQVVQALKKLGLSVYMLTGDNQATAQAIADTVGIRHVLAQVRPGDKAQQVEQLQQKGNIVAMVGDGINDAPALAQADVGIAIGTGTDVAIAASDITLIAGDLQGILTAIKLSRATMGNIRQNLFFAFIYNVIGIPVAAGLFYPLFGLLLNPILAGAAMAFSSVSVVTNALRLKKFCP p73213_syny3 ssr2857 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MTIQLTVPTIACEACAEAVTKAVQNEDAQATVQVDLTSKKVTITSALGEEQLRTAIASAGHEVE p74512_syny3 slr1950 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MVQLSPTPASTLTYKDANGQNRTASLTLDVGGMKCAGCVAAVERQLDQLTGVTDSCVNLVTAVAVVRYEPEKIQPQAIAEHLSQRGFPSQIRHGHGAIPATIGEKETRENVNWGLTIALVLLLLSGLGHLSHFGGPMIPFFHHPIFHWSLATLAIAIPGREIFLDGWRGLRFGHANMNTLVALGTGSAYLTSCIAWAWPGLGWECFFDEPVMLLGMLLLGRTLESKARQKAKSALTELLALQPSLARLVGRGEDQGETGIEIPVEQVRVGEWVQVLPGEKIPVDGILVAGKTLVDESLLTGESLPVAKNVDDAVIAGAWNQSGAIIIAATHIGAETTLARIIQLVETAQTQKAPMQRLADQVAGWFAYGVLAIALVTLGFWAMVGQSLFPEMVADTGLSPLLLALKLSVSVLVVACPCALGLATPTAILVGTSLGAEQGILIKGGNILEILQRTTVMAFDKTGTLTQGNLQLTDAVPVADITGIELLTLAASVEQGTRHPLAQGLISSAQGLELLPVENIETEAGQGVQGWYQGDRLLVGNQQWLMEQGVMGEPQWQTAVDQLLDQGKTVIFVARNQQLQGFLALRDTLRPEAKATIAQLKQWGIAPLLLTGDHPAIAQAIAMEVGIEEFQAQMTPQAKVAKIKAMQGFNPVSVIAMVGDGINDAPALAQADVGISLSGATAVAMETADVVLMRSHLSDVLKALTLSRSTVAKIKQNLLWALGYNLLAIPLAAGAFLPSFAIVLTPAIAAAMMASSSIVVVLNALALRYQFSRSLGQDPG pacs-ssr2857-2 bacterioMatch interaction of PacS and atx1 (ssr2857) of Synechocystis sp. (strain PCC 6803) 358753 N/A 358755 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 358756 unspecified role unspecified role prey prey P73213:p.[Cys12Ser;Cys15Ser] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false slr1950-ssr2857-2 bacterioMatch interaction of CtaA (slr1950) and atx1 (ssr2857) of Synechocystis sp. (strain PCC 6803) 358753 N/A 358757 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 358756 unspecified role unspecified role prey prey physical association physical association false false false ssr2857-slr1950-1 Copper transfert from atx1 (ssr2857) to CtaA (slr1950) detected in an anion exchange column. 358754 N/A 358757 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 358756 enzyme enzyme unspecified role unspecified role ecobb Escherichia coli (strain B / BL21) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false P73213 purified P74512 purified