IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ papoulas-1998-1 The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. chromatography chromatography technology column chromatography chromatography de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. Development (0950-1991) 1998 Only protein-protein interactions imex curation papoulas-1998-2 The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. Development (0950-1991) 1998 Only protein-protein interactions imex curation papoulas-1998-3 The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. Development (0950-1991) 1998 Only protein-protein interactions imex curation hsp7d_drome Heat shock 70 kDa protein cognate 4 Hsc70-4 Hsc4 CG4264 Heat shock 70 kDa protein 88E protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTQTIFDAKRLIGRKFDDAAVQSDMKHWPFEVVSADGKPKIEVTYKDEKKTFFPEEISSMVLTKMKETAEAYLGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFDLGGGTFDVSILSIDDGIFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGTDFYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEKYRNEDEKQKETIAAKNGLESYCFNMKATLDEDNLKTKISDSDRTTILDKCNETIKWLDANQLADKEEYEHRQKELEGVCNPIITKLYQGAGFPPGGMPGGPGGMPGAAGAAGAAGAGGAGPTIEEVD bap60_drome Brahma-associated protein of 60 kDa Bap60 BRM-associated protein 60 CG4303 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSQRFAPGQAPVQSRYQPPPPAPGMRPYPPPGASFPPRGFPLHPNTTQPVPGTANVAGVPGVPGVPGVPGPSLLQRAAQQPGFQSSLRGTGAGGGGVGSGGGSKRSNESRSLGGGGSKSDFATAKKKKKLAEKILPQKVRDLVPESQAYMDLLTFERKLDATIMRKRLDIQEALKRPMKQKRKLRIFISNTFYPSKEPTNDGEEGAVASWELRVEGRLLEDGKGDPNTKIKRKFSSFFKSLVIELDKELYGPDNHLVEWHRTHTTQETDGFQVKRPGDRNVRCTILLLLDYQPLQFKLDPRLARLLGVHTQTRPVIISALWQYIKTHKLQDAHEREYINCDKYLEQIFSCQRMKFAEIPQRLNPLLHPPDPIVINHFIESGAENKQTACYDIDVEVDDTLKNQMNSFLMSTASQQEIQGLDTKIHETVDTINQMKTNREFFLSFAKDPQMFIHRWIISETRDLKLMTDVAGNPEEERRAEFYYQPWTHEAVSRYFFTKVNQKRAELEQALGIRNG q7k012_drome Bap55 ARP4 Arp4 BAF53 BAP47 BAP55 Baf53 Dmel\CG6546 bap55 CG6546 Dmel_CG6546 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGGTMLYGGDEIGALVFDPGHHSLRVGYAQEDSPKAEIPSVVGIGAAPETNLDPETKTDNNATPNNADQRKFYVDTNYVTVPRSNMEVQTYMKDGMIDNWDLFEKVIDYAYANVIQSEPEYHPVLFSEASWNVRNNREKLTELMFEKYNVPAFFLVKNAVLAAFSSGRATALVVDSGATHTSAVPVHEGYVLSQAVVKSPLGGDFLSRQCRQHLEKHGIDLSPVYKIASKDVVKERDNGRFTLRKLPENLTQSWQNYMLQLMMQDFQMNVLQVLENPFDERVAAQIPTVHYEFPNGYHQDFGSERFKIAESLFDNAMLGAGQLASTSVGMCDADVRLSLFGSVVVTGGNTLLQGFPERLNRDLQLRAPSNTRLKMISANGSVERRFGAWIGGSILASIGTFQQMWISSQEYEEAGKSQVERKCP act1_drome Actin-5C Act5C CG4027 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCDEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF q24090_drome Snr1 BAP45 Dmel\CG1064 SNR1 Snr Snr-1 l(3)01319 snr snr-1 snr1 Dmel_CG1064 CG1064 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALQTYGDKPVAFQLEEGGEYYYVGSEVGNYMRHFRGILYKKYPGMTRIVLSNEERKRLAESGLSSHILASSVSLLRAVEVDDIMAGNDEKYRAVSVNTSDTPVPRESKSKKQPQYVPTMPNSSHLDAVPQATPINRNRVHTKKVRTFPMCFDDTDPTASLENAAQKECLVPIRLDMELEGQKLRDTFTWNKNESMITPEQFAEVLCDDLDLNPLPFVPAIAQAIRQQIEAFPNDPPILEETCDQRVIVKLNIHVGNTSLVDQVEWDMSEKNNNPEEFAIKLCAELGLGGEFVTAIAYSIRGQLSWHCRTYAFSEAPLSTIDVPFRNPSDADAWAPFLETLTDAEMEKKIRDQDRNTRRMRRLANTTTGW q9vf03_drome Dmel_CG18740 BAP155 Dmel\CG18740 E(E2F)3B MOIRA MOR Moi Moira Mor Swi3D dmMOIRA l(3)89B1 mor CG4275 CG18740 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNTLGPKKDGSPNIDFFQSPETLQGFESIRQWLQKNCKKYLAHSSEPITKESLAQLLIHFLQYVEAKLGKNSADPPATRIPMRCFLDFKSGGGLCIIFSTMFRFRAEQRGKKFDFSIGKNPTRKDPNIQLLIEIEQALVEADLYRIPYIYIRPEIEKGFEGKLREILDNRRVEIVSDEEDATHIVYPVVDPHPDEYARPIFKRGGHVMLHWYYFPESYDSWAVNNFDLPDHIPENPESPAERWRVSASWIVDLEQYNEWMAEEDYEVDEQGKKKTHKQRMSIDDIMSFGDEKKKPAASSGGGKQKRRRSPSPASSASTSKPGKRKRSPAVVHKKSRNDDDDEDLTRDLDDPPAEPNVQEVHKANAALQSTASPAPGGKSRGDNDMMPIKGGTMTDLDDEMTGGSAAQAMSTGDGENSQTGKTSDNSNTQEFSSSAKEDMEDNVTEQTHHIIVPSYSAWFDYNSIHVIEKRAMPEFFNSKNKSKTPEIYMAYRNFMIDTYRLNPTEYLTSTACRRNLAGDVCAIMRVHAFLEQWGLINYQIDADVRPTPMGPPPTSHFHILSDTPSGLQSINPQKTQQPSAAKTLLDLDKKPLGKDGGLELGDKSGLTGIKTEALENGAAGGLSSGVSQFGLKLDQYAKKPAAMRNRTAASMAREWTDQETLLLLEGLEMHKDDWNKVCEHVGSRTQDECILHFLRLPIEDPYLEDDGGFLGPLACQPIPFSKSGNPIMSTVAFLASVVDPRVAAAAAKAAMEEFAAIKDEVPATIMDNHLKNVEKASAGGKFNPNFGLANSGIAGTGNDKDDEEGKEGGASASAGGSDEEMKDLSKKDDDAKSKDNTKSDKTNTNTDSSSTSSSATGNTNNTDKKPKESSGSSPSGDKSAIKSDKSNKSSPTETAAAASGGEVDIKTEDSSGDGETKDGTEAKEGTGTGTGPLAVPKEGTFSENNMQTAAAAALASAAVKAKHLAALEERKIKSLVALLVETQMKKLEIKLRHFEELEATMEREREGLEYQRQQLITERQQFHLEQLKAAEFRARQQAHHRLQQELQGQAAAGSMILPQQQQLPQAQQPQQQQQTLPPHPHLAQQQQLPPHPHQLPPQSQPLAGPTAQHQPLPPHVVSSPNGAPFAAPPISLTGGLPPGAPTAIVTNPSDQTGPVAAGAAQSQAPTPMDTTPPSSGPVSDANAPPGSAMPPGGIPPANTAPIAGVAPSS q9biw6_drome Bap111 dalao CG7055 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALPSNYKQIAVGGQGSATPLQGGGGGSGGGSRSRSSGGGGGGDRNKDQTPIFTHSNYGNPAFTPQKVTKSSSSKNQNESRLPKPPKPPEKPILPYMRYSKRVWDSVKAKHPELKLWELGKKIGAMWKLLPEDEKTEFIDEYEAEKLEYEKSLKAYHQTPAYQAYMSAKSKVKTDVDMHETPSRGGGSKSQHERRIDIQPAEDEDDQDEGYTTKHLAYARYLHNHRLINEIFSEAVVPDVRSVVTTTRMQVLKRQVSSLTMHQTKLEAELQQMEEKFEAKKQRMVESSEAFQEELKRHCKPAVDEETFQKMVLRMYEDIKRDRQRLDEPNANANSAANPAAAAATAAAAPVTRSEEAVKPPTQPGQPAATPAGQEPASAVPAPAAPPKETPPAVKPATLNPTPSSTPTPAPAVHVHETASKTDPEPMDIEPPPKPSVPPPPIKPEKLEMAAALPPQSTLVEPPKTEPAKVVAQPGKVPTPVPTPTPPPEVAPAAGAVTAATTTTRRTPTPPPVPQQVPLPQQQGGPAPPPGMPQMHPHPGHPPPGHSLMPPHMGPHQPPPGMPGLPPPPPHTGYANYGGPPHGPPPGPPGGPARPYYQPQYGGHPTPQPYYAPFSPYQQSYGPPPGSHYMSPRPPPPQHNGNPGHPYAPEHGSNPPPPQQQQQQQPPPGHLHEPSGGGPGAPGGGAGAAAAAAPGAGVYPTPGAGAGPGAPPGPAGGAPLGEAAVAGGVGPPAATALGKHPEAEKHEAD brm_drome ATP-dependent helicase brm Homeotic gene regulator Protein brahma brm CG5942 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMIFSKGPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD brm-bap55 654194 N/A 654197 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false n/a 654198 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false n/a 654199 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false N/A 654200 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false N/A 654201 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false N/A 654202 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false N/A 654203 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false N/A 654204 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Table 1 brm-snr1-1 654195 N/A 654201 unspecified role unspecified role prey prey N/A 654204 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 5C brm-snr1-2 654196 N/A 654204 unspecified role unspecified role bait bait c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 654201 unspecified role unspecified role prey prey physical association physical association false false false 5B