IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ thommes-1997-5 The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. xenla-oocyte Xenopus laevis oocyte Oocyte cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Thommes P., Kubota Y., Takisawa H., Blow JJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Interphase extract was analysed on 20�40% glycerol gradients. EMBO J. (0261-4189) 1997 thommes-1997-6 The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. xenla-oocyte Xenopus laevis oocyte Oocyte chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Thommes P., Kubota Y., Takisawa H., Blow JJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Interphase extract was analysed on 20�40% glycerol gradients. EMBO J. (0261-4189) 1997 thommes-1997-1 The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. xenla-oocyte Xenopus laevis oocyte Oocyte chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Thommes P., Kubota Y., Takisawa H., Blow JJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Interphase extract was analysed on 20�40% glycerol gradients. EMBO J. (0261-4189) 1997 thommes-1997-2 The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Thommes P., Kubota Y., Takisawa H., Blow JJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Interphase extract was analysed on 20�40% glycerol gradients. EMBO J. (0261-4189) 1997 thommes-1997-3 The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Thommes P., Kubota Y., Takisawa H., Blow JJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Interphase extract was analysed on 20�40% glycerol gradients. EMBO J. (0261-4189) 1997 thommes-1997-4 The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides. xenla-oocyte Xenopus laevis oocyte Oocyte cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Thommes P., Kubota Y., Takisawa H., Blow JJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Interphase extract was analysed on 20�40% glycerol gradients. EMBO J. (0261-4189) 1997 mcm4a_xenla DNA replication licensing factor mcm4-A mcm4-a Minichromosome maintenance protein 4-A CDC21 homolog-A P1-CDC21-A p98 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSSPTSTPSRRKSKRGRGSNPPTPRGEEVQSPPSQKRRTEDSTSIGELLPMPTSPSGDIQSPLFSSPAPSRHSAHQSELDLSSPLTYGTPSSRVEGTPRSGIRGTPARQRADLGSARKVKQVDLHSDQPAAEELVTSEQSLGQKLVIWGTDVNVAICKEKFQRFVQRFIDPLAKEEENVGLDLNEPIYMQRLEEINVVGEPFLNIDCDHLRSFDQDLYRQLVCYPQEVIPTFDMAANEIFFERYPDSILEHQIQVRPYNALKTRNMRSLNPEDIDQLITISGMVIRTSQIIPEMQESFFKCQVCAFTTRVEIDRGRIAEPSVCKHCNTTHSMALIHNRSMFSDKQMIKLQESPEDMPAGQTPHTTILYAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVRNVKSVYKTHIDVIHYRKTDSKRLHGIDEDTEQKMFTEERVAVLKELAAKPDIYERLAAALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEVNILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDETYDRRLAHHLVALYYQSEEQLKEEHLDMAVLKDYIAYARTYVNPRLGEEASQALIEAYVDMRKIGSGRGMVSAYPRQLESLIRLSEAHAKVRFSSKVETIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPAFKYQQLFEDLRGQSDAAITKDMFDEALHALADEDYLTVTGKTVRLL mcm3m_xenla Maternal DNA replication licensing factor mcm3 Maternal minichromosome maintenance protein 3 P1 homolog p100 XRLF subunit beta mmcm3 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDYGGGFEDHELREAQREYLDFLDDDQDQGLYHGKVRDMIGSNEHRLIVNLNDVRRKNDKRANLMLNDAFAETIAFQRALKDLVASIDATYAKQFEEFSVGFEGSFGSKHVSPRTLTASLLGSLVCVEGIVTKCSLVRPKVMRSVHYCPATKKTLERKYSDLTSLEAFPSSSIYPTKDEENNPLETEYGLSTYKDHQTLSIQEMPEKAPAGQLPRSVDIIADDDLVDKCKPGDRVQIVGIYRCLPSKQGGFTSGTFRTILLANNIKLMSKEIAPTFSADDVAKIKKFCKAHSKDIFEHLSKSLAPSIHGHEYIKKAILCMLLGGNEKVLENGTRIRGDINVLLIGDPSVAKSQLLRYVLHTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIQARLNARCSVLAAANPVYGRYDQYRTPMENIGLQDSLLSRFDLLFIVLDKMDADNDQEIADHVLRMHRYRTPGEQDGYALPLGCSVEIFATDDPNASDVTDQELQIYEKHDNLLHGPRKNKSKIVSMQFIRKYIHVAKLIKPVLTSEAADYISQEYAKIRNHDQINNDSARTMPVTARALETMIRLSTAHAKVRMSKTIERQDAETALELVQFAYFKKVLAKEKKKTDKDLHDENLSQDTLSQESVRKSSRRAGKIADSQDDSMDPYSFSEQDSSLNENLSQSLRPQRKKAESQDGKRSLSQNRTKEFKAALLKAFKSSRSQSVAVSQLLELINKGNPEPFERSEVKEALDNMQNDNQVMVSEDVVFLI mcm7a_xenla DNA replication licensing factor mcm7-A Minichromosome maintenance protein 7-A CDC47 homolog A CDC47p p90 mcm7-a cdc47 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MPRDYQTEKEKCKTFLQEFYKDDEIGKKHFKYGVQLANIAHREQVALYIDLDDLAEEDPELVDAICENTRRYTNLFADAVQELLPQYKEREVVHKDALDVYIEHRLMMEQRGRDPNEMRDSQNQYPPELMRRFELYFKAPSSSKARVVRDVKADSIGKLVNIRGIVTRVTEVKPMMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSRECQTNRSGGRLYLQTRGSKFIKFQELKIQEHSDQVPVGNIPRCMSVYVRGENTRLAQPGDHVGITGVFLPMLRTGFRQVVQGLLSETYLECHRLVKMNKSEDDELGTEELSEEELRQITEEDFYEKLAASIAPEIYGHEDVKKALLLLLVGGVDNSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVMKDPVTGEMTLEGGALVLADQGVCCIDEFDKMMDTDRTAIHEVMEQQTISIAKAGIMTTLNARCSILAAANPAYGRYNPKKTVEQNIQLPAALLSRFDVLWLIQDKPDRDNDLRLAQHITYVHQHSKQPPSQFQPLDMKLMRRYITMCKRKQPAIPEALADYLTAAYVEMRKEARTNKDMTFTSARTLLSVLRLSTALARLRLEDVVEKEDVNEAMRLMEMSKDSLLGDKGHTSRTQRPADVIFSTIREMVPEKGARSVKYSEAEQRCVSKGFTPAQFEAALEEYEELNVWLVNQARTKITFV mcm2_xenla DNA replication licensing factor mcm2 Minichromosome maintenance protein 2 BM28-homolog p112 mcm2 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MADSSESFNIATSPRAGSRRDALTSSPGRDLPPFEDESEGMFGDGVVPEEEEDGEELIGDAMERDYRPISELDRYEVEGLDDEEDVEDLTASQREAAEQSMRMRDREMGRELGRMRRGLLYDSDEEEEDRPARKRRMAERAAEGAPEEDEEMIESIENLEDMKGHTVREWVSMAATRLEIYHRFKNFLRTHVDEHGHNVFKEKISDMCKENKESLPVNYEDLAAREHVLAYFLPEAPAEMLKIFDEAAKEVVLVMYPKYDRIAREIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTCCTGVLPQLSMVKYNCNKCNFILGPFFQSQNQEVRPGSCPECQSFGPFEINMEETVYQNYQRITIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHITKKDDKVAVGELTDEDVKAIVALSKDERIGERIFASIAPSIYGHEDIKRGLALALFGGEAKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYVEKVASRAVFTTGQGASAVGLTAYVQRHPVTKEWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAASNPIGGRYDPSLTFSENVDLTEPIVSRFDILCVVRDTVDPVQDEMLARFVVSSHIKHHPSSKDIANGDAAEFALPNTFGVEALPQEVLKKYIMYAKEKIRPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARMHLRDYVVEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLAFRRDNNELLLFVLKQLIAEQVTYQRNRYGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSDLFKMNKFTHDVKKKLIIQQF mcm5a_xenla DNA replication licensing factor mcm5-A CDC46 homolog A CDC46p p92 mcm5-a cdc46-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGFDDLGVYYSDSFGGEQQVGDDGQAKKSQLKKRFREFLRQYRIGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPTEHLQLLEEAAQEVADEVTRPRPAGEETIQEIQVMLRSDANPANIRSLKSEQMSHLVKIPGIIIAATAVRAKATKISIQCRSCRNTIGNIAVRPGLEGYAMPRKCNTEQAGRPNCPLDPYFIIPDKCKCVDFQTLKLQESPDAVPHGELPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIRKSGKTSTKGRDRVGVGIRSSYIRVVGIQVDTEGTGRSAAGAITPQEEEEFRRLAAKPDIYETVAKSIAPSIYGSSDIKKAIACLLFGGSRKRLPDGLTRRGDVNLLMLGDPGTAKSQLLKFVERCSPIGVYTSGKGSSAAGLTASVMRDPVSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVYGRWDDTKGEENIDFMPTILSRFDMIFIVKDEHNEQRDMTLAKHVMNVHLSARTQSSSVEGEVDLNTLKKYIAYCRAKCGPRLSAEAAEKLKNRYILMRSGAREHERETEKRSSIPITVRQLEAIVRISESLGKMKLQPFATETDVEEALRLFQVSTLDAAMSGSLSGVEGFTTQEDQEMLSRIEKQMKKRFAIGSQVSEHSIIQDFLKQKYPEHAIHKVLSLMMRRGEIQHRLQRKVLYRIK mcm6m_xenla Maternal DNA replication licensing factor mcm6 mmcm6 Maternal minichromosome maintenance protein 6 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELGGPAAAGDTDIAGQQLFKDELSDKCQKLFLEFLEECKGKDGSNLYVSAAEELIRPERNTLAVNFTDIEYYNQQLATTIQEEYYRVYPHLCRAVRSFARQMGNIPANKEFYIAFSDFPARQKIRELSSAKIGTLLRISGQVVRTHPVHPELVSGTFLCMDCQSIVKDVEQQFRYTQPTICKNPVCANRRRFTLDTNKSRFVDFQKVRIQETQAELPRGAIPRSVEIILRAEAVESAMAGDRCDFTGTLIVVPDVSALAAGDARMETGAKVTGGEGFNSEGVQGLKALGVRDLSYRLAFLACYVGATNPRFGGKDLREEDQTAESIKNQMTVQEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEIKRGVLLMLFGGVPKTTMEGTSLRGDINVCIVGDPSTSKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVKDEESHEFVIEAGALMLADNGVCCIDEFDKMDLKDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPVGGRYERSKSLKHNVNLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHARNEESIERVYSIEDIQRYLLFARQFQPKITKEAEEFIVEQYRRLRQRDGSGVAKSSWRITVRQLESLIRLSESMARMHCSDEVQPKHVKEAFRLLSKSIIRVDTPDVSFDQGEDEKNIEGENNGNLNNGEEAMETNQDEPINEKPSSNAGLKMSFAEYKQISNLLVLYMQKMEETEEECHLTTTDLVNWYLKEMEAEIETETELILKKRLIEKVIHRLIYYDHILIELNKSELKTMDDTKETGEDAAEDRILVVNPNYMLED mcm3-mcm2-4 744498 N/A 744504 unspecified role unspecified role neutral component neutral component N/A 744505 unspecified role unspecified role neutral component neutral component N/A 744506 unspecified role unspecified role neutral component neutral component N/A 744507 unspecified role unspecified role neutral component neutral component N/A 744508 unspecified role unspecified role neutral component neutral component N/A 744509 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4A. 4B Similar results were opbserved with purified RLF-M fraction mcm3-mcm2-5 744498 N/A 744505 unspecified role unspecified role neutral component neutral component N/A 744507 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4A, 4B Similar results were opbserved with purified RLF-M fraction mcm3-mcm2-6 744499 N/A 744506 unspecified role unspecified role neutral component neutral component N/A 744509 unspecified role unspecified role neutral component neutral component N/A 744508 unspecified role unspecified role neutral component neutral component N/A 744504 unspecified role unspecified role neutral component neutral component N/A 744505 unspecified role unspecified role neutral component neutral component N/A 744507 unspecified role unspecified role neutral component neutral component physical association physical association false false false 5 mcm3-mcm2-7 744499 N/A 744505 unspecified role unspecified role neutral component neutral component N/A 744508 unspecified role unspecified role neutral component neutral component physical association physical association false false false 5 mcm3-mcm2 744500 N/A 744508 unspecified role unspecified role neutral component neutral component N/A 744507 unspecified role unspecified role neutral component neutral component N/A 744505 unspecified role unspecified role neutral component neutral component N/A 744506 unspecified role unspecified role neutral component neutral component N/A 744504 unspecified role unspecified role neutral component neutral component N/A 744509 unspecified role unspecified role neutral component neutral component physical association physical association false false false 1 and 2A, 4C, 4D Simmilar results were observed in RLF-M compelxes. mcm3-mcm2-1 744501 N/A 744506 unspecified role unspecified role prey prey N/A 744508 unspecified role unspecified role prey prey N/A 744504 unspecified role unspecified role prey prey N/A 744507 unspecified role unspecified role prey prey N/A 744505 unspecified role unspecified role bait bait N/A 744509 unspecified role unspecified role prey prey association association false false false 2B mcm3-mcm2-2 744502 N/A 744507 unspecified role unspecified role prey prey N/A 744505 unspecified role unspecified role bait bait N/A 744508 unspecified role unspecified role prey prey association association false false false 2C mcm3-mcm2-3 744503 N/A 744508 unspecified role unspecified role neutral component neutral component N/A 744506 unspecified role unspecified role neutral component neutral component N/A 744509 unspecified role unspecified role neutral component neutral component N/A 744507 unspecified role unspecified role neutral component neutral component N/A 744504 unspecified role unspecified role neutral component neutral component N/A 744505 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4