IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ito-2012-2 Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation ito-2012-3 Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation ito-2012-5 Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. drome-salivary_gland Drosophila melanogaster Salivary gland salivary_gland Salivary gland fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation ito-2012-1 Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. drome-head Drosophila melanogaster head anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation ito-2012-4 Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. drome-larva Drosophila melanogaster larva larva Immature form of an organism anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ito H., Sato K., Koganezawa M., Ote M., Matsumoto K., Hama C., Yamamoto D. Chromatin - Epigenetic interactions resulting in chromatin modulation daichan@m.tohoku.ac.jp Cell (0092-8674) 2012 Only protein-protein interactions imex curation q8in81-6 Sex determination protein fruitless B fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMATSQDYFGNPYALFRGPPTTLRPRESPLGVGHPHGHGHLHSHAHAHGHGHAHSHYAALDLQTPHKRNIETDVRAPPPPLPPPPLPLPPASPRYNTDQGAMDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGSSARHHLSTPLSTSSSASPPPPPFGMHLSAALKREYHPLHYMAAGNGHNGPSALGYGNQGSGNAPNSAGGAGSVAGGVGAGGGAGGATGAAGHNSHHTMSYHNMFTPSRDPGTMWRCRSCGKEVTNRWHHFHSHTAQRSMCPYCPATYSRIDTLRSHLRVKHPDRLLKLNSSI q9vdk5_drome bon CG15687 CG5206 0241 0249 0434 0487 06 08 12 150268_at 150269_at 20 Bon Bonus Dmel\CG5206 anon-WO0118547.418 l(3)S043420 Dmel_CG5206 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSGKVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNVSAMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEVNNKNEQKDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQEDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE hdac1_drome Histone deacetylase HDAC1 HDAC1 Rpd3 CG7471 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQSHSKKRVCYYYDSDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATADEMTKFHSDEYVRFLRSIRPDNMSEYNKQMQRFNVGEDCPVFDGLYEFCQLSAGGSVAAAVKLNKQASEICINWGGGLHHAKKSEASGFCYVNDIVLGILELLKYHQRVLYIDIDVHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNIPLRDGMDDDAYESIFVPIISKVMETFQPAAVVLQCGADSLTGDRLGCFNLTVKGHGKCVEFVKKYNLPFLMVGGGGYTIRNVSRCWTYETSVALAVEIANELPYNDYFEYFGPDFKLHISPSNMTNQNTSEYLEKIKNRLFENLRMLPHAPGVQIQAIPEDAINDESDDEDKVDKDDRLPQSDKDKRIVPENEYSDSEDEGEGGRRDNRSYKGQRKRPRLDKDTNSNKASSETSSEIKDEKEKGDGADGEESTASNTNSNNNSNNKSDNDAGATANAGSGSGSGSGAGAKGAKENNI hp1_drome Heterochromatin protein 1 Su(var)205 HP1 CG8409 Non-histone chromosomal protein C1A9 antigen protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGKKIDNPESSAKVSDAEEEEEEYAVEKIIDRRVRKGKVEYYLKWKGYPETENTWEPENNLDCQDLIQQYEASRKDEEKSAASKKDRPSSSAKAKETQGRASSSTSTASKRKSEEPTAPSGNKSKRTTDAEQDTIPVSGSTGFDRGLEAEKILGASDNNGRLTFLIQFKGVDQAEMVPSSVANEKIPRMVIHFYEERLSWYSDNED q8in81-1 Sex determination protein fruitless E fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMATSQDYFGNPYALFRGPPTTLRPRESPLGVGHPHGHGHLHSHAHAHGHGHAHSHYAALDLQTPHKRNIETDVRAPPPPLPPPPLPLPPASPRYNTDQGAMDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGRVKCFNIKHDRHPDRELDRNHREHDDDPGVIEEVVVDHVREMEAGNEHDPEEMKEAAYHATPPKYRRAVVYAPPHPDEEAASGSGSDIYVDGGYNCEYKCKELNMRAIRCSRQQHMMSHYSPHHPHHRSLIDCPAEAAYSPPVANNQAYLASNGAVQQLDLSTYHGHANHQLHQHPPSATHPSHSQSSPHYPSASGAGAGAGSVSVSIAGSASGSATSAPASVATSAVSPQPSSSSTGSTSSAAAVAAAAAAAANRRDHNIDYSTLFVQLSGTLPTLYRCVSCNKIVSNRWHHANIHRPQSHECPVCGQKFTRRDNMKAHCKIKHADIKDRFFSHYVHM q8in81-9 Sex determination protein fruitless A fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGE fru_drome Sex determination protein fruitless fru BTB-VI CG14307 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMATSQDYFGNPYALFRGPPTTLRPRESPLGVGHPHGHGHLHSHAHAHGHGHAHSHYAALDLQTPHKRNIETDVRAPPPPLPPPPLPLPPASPRYNTDQGAMDQQFCLRWNNHPTNLTGVLTSLLQREALCDVTLACEGETVKAHQTILSACSPYFETIFLQNQHPHPIIYLKDVRYSEMRSLLDFMYKGEVNVGQSSLPMFLKTAESLQVRGLTDNNNLNYRSDCDKLRDSAASSPTGRGPSNYTGGLGGAGGVADAMRESRDSLRSRCERDLRDELTQRSSSSMSERSSAAAAAAAAAAAVAAAGGNVNAAAVALGLTTPTGGERSPSVGSASAAAAAAAVAAAVAAAANRSASADGCSDRGSERGTLERTDSRDDLLQLDYSNKDNNNSNSSSTGGNNNNNNNNNNNSSSNNNNSSSNRERNNSGERERERERERERDRDRELSTTPVEQLSSSKRRRKNSSSNCDNSLSSSHQDRHYPQDSQANFKSSPVPKTGGSTSESEDAGGRHDSPLSMTTSVHLGGGGGNVGAASALSGLSQSLSIKQELMDAQQQQQHREHHVALPPDYLPSAALKLHAEDMSTLLTQHALQAADARDEHNDAKQLQLDQTDNIDGRVKCFNIKHDRHPDRELDRNHREHDDDPGVIEEVVVDHVREMEAGNEHDPEEMKEAAYHATPPKYRRAVVYAPPHPDEEAASGSGSDIYVDGGYNCEYKCKELNMRAIRCSRQQHMMSHYSPHHPHHRSLIDCPAEAAYSPPVANNQAYLASNGAVQQLDLSTYHGHANHQLHQHPPSATHPSHSQSSPHYPSASGAGAGAGSVSVSIAGSASGSATSAPASVATSAVSPQPSSSSTGSTSSAAAVAAAAAAAANRRDHNIDYSTLFVQLSGTLPTLYRCVSCNKIVSNRWHHANIHRPQSHECPVCGQKFTRRDNMKAHCKIKHADIKDRFFSHYVHM fru-bon-2 1300746 n/a 1300751 unspecified role unspecified role bait bait n/a 1300752 unspecified role unspecified role prey prey n/a 1300753 unspecified role unspecified role prey prey n/a 1300754 unspecified role unspecified role prey prey association association false false false Fig. 3F fru-rpd3-1 1300746 n/a 1300754 unspecified role unspecified role prey prey n/a 1300752 unspecified role unspecified role ancillary ancillary n/a 1300751 unspecified role unspecified role bait bait n/a 1300753 unspecified role unspecified role prey prey association association false false false Fig. 3G, 3H fru-rpd3-2 1300746 n/a 1300754 unspecified role unspecified role prey prey n/a 1300753 competitor competitor competitor prey prey n/a 1300751 unspecified role unspecified role bait bait association association false false false Fig. 3I Heat-shock bon also present but not shown to be required for the interaction fru-rpd3-3 1300746 n/a 1300751 unspecified role unspecified role bait bait n/a 1300752 unspecified role unspecified role ancillary ancillary n/a 1300754 unspecified role unspecified role prey prey association association false false false Fig. 3I bon-fru 1300747 n/a 1300752 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1300755 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 2B bon-fru-1 1300747 n/a 1300752 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1300756 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 2B bon-fru-2 1300747 n/a 1300752 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1300751 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 2B su_var_205-bon 1300747 n/a 1300754 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1300752 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 2D bon-fru-3 1300748 n/a 1300757 unspecified role unspecified role neutral component neutral component n/a 1300752 unspecified role unspecified role neutral component neutral component n/a 1300753 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4A, 4B Co-localization not observed in bon-/- cells bon-fru-5 1300748 n/a 1300754 unspecified role unspecified role neutral component neutral component n/a 1300757 unspecified role unspecified role neutral component neutral component n/a 1300752 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4A, 4B Co-localization not observed in bon-/- cells fru-bon 1300749 n/a 1300754 unspecified role unspecified role prey prey n/a 1300753 unspecified role unspecified role prey prey n/a 1300752 unspecified role unspecified role prey prey n/a 1300755 unspecified role unspecified role bait bait association association false false false Fig. 3B-D Heat-shock The proportion of Fru-expressing neurons represents less than 2% of all neurons in the brain. To increase the sensitivity of detection, heat-shockable-fruEM was overexpressed in adult flies rpd3-bon 1300750 n/a 1300753 unspecified role unspecified role bait bait n/a 1300752 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 2C