IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ li-2008c-2 Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li F., Huarte M., Zaratiegui M., Vaughn MW., Shi Y., Martienssen R., Cande WZ. Chromatin - Epigenetic interactions resulting in chromatin modulation Details of histone peptide supplied by author and from manufacturer's website. Peptide sequence has a 100% match to both S. pombe H3 proteins so both have been shown as binding to Lid3. feili@nature.berkeley.edu, zcande@berkeley.edu Cell (0092-8674) 2008 Only protein-protein interactions 9 imex curation li-2008c-3 Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li F., Huarte M., Zaratiegui M., Vaughn MW., Shi Y., Martienssen R., Cande WZ. Chromatin - Epigenetic interactions resulting in chromatin modulation Details of histone peptide supplied by author and from manufacturer's website. Peptide sequence has a 100% match to both S. pombe H3 proteins so both have been shown as binding to Lid3. feili@nature.berkeley.edu, zcande@berkeley.edu Cell (0092-8674) 2008 Only protein-protein interactions 9 imex curation li-2008c-1 Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Li F., Huarte M., Zaratiegui M., Vaughn MW., Shi Y., Martienssen R., Cande WZ. Chromatin - Epigenetic interactions resulting in chromatin modulation Details of histone peptide supplied by author and from manufacturer's website. Peptide sequence has a 100% match to both S. pombe H3 proteins so both have been shown as binding to Lid3. feili@nature.berkeley.edu, zcande@berkeley.edu Cell (0092-8674) 2008 Only protein-protein interactions 9 imex curation raf1_schpo Rik1-associated factor 1 SPCC613.12c Cryptic loci regulator 8 De-localization of swi6 protein 1 raf1 clr8 cmc1 dos1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTNSSPRVKRRTDTQYLHSVSSKLPKVDFDTNNEEFFEEEFEIYDPFYRAELPCPKPSLSISKHSIAKVPSNVNKRLELQLLLTSGTFLPNSRPYLSERVRKHTHLLSNSITGDDKPSLIHVDFTPEECFILQEAKLKFGPVNSVQFNDAYSTHISPKLPGRAYEDCQKFEIDNPSLSPVDKHGAIILRTYKKNKKLLPDYLKSFYNAGSSYFQREQVHQLMDGESVFFLKPWKHFNETSGDTVCVAYNPLCEKFALGSTAQDGAYNRLGNLWIGDFHSETIQSLESHYKLNQVGEKEYSTISDLCFSKGNLFLYTGAFDNAVKVWDMEGNLCGIFNAPTDYIHKLALSDDDLLAVACKNGYGYLLSTDNSTGEILTSANLIYPEALEKGYSASLIEFSNFLGRSSDKVIIGYDSFHTSNNRGCLALFDASTASFVQKFNTADEAFTSLYMHPSQVGFVASSNTLSNGRVYYLDTRMYKVCLNFTTTQKDINHATISNSGILVTSSGTDNQTFVWDSRKPDKPLSLLKHGKTKMIHFDGANEEEVDAGINMAQWQPKGNLFVTGGSDGIVKVWDLRLNNPFIQNFTEMNSAITYGGFSEDASKLTVCCVGGDVNMYSLGNDNGNKFGEFRIIENRLLT 6CD360D8748AAF98 lid2_schpo Lid2 complex component lid2 lid2 SPBP19A11.06 SPBP4H10.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MICQLLWHEHNSMGSGREKKIRKLGDNFSLPYLKFDCDHNDKNYRASNRPFGLSTGLSVQLNASNMTDPFKFLLDNWHTIFKNGAIKLLPPEGWQIPVVLDQGAFEFQSKRQCLNKGCLNYEKNYDYFKKLKAFHESRGLYFYHPPIIGNRPVDFLRLRNAISKFTNSGSSLNNEILHKVIIYLRLEDTKEVRQVLTRCYDRYIKPFERDSSPSFKSKRSESSTRKIRNTRSSAQQESPIPETSAQSPVQTIQVNGSTSLKRPLIERGEQCEYCGLDKNPETILLCDGCEAAYHTSCLDPPLTSIPKEDWYCDACKFNISDYDPRKGFKWKLSSLKERSAEIFNTLGERNSSSKLTNLTEDDIELFYWSSLAESNSGFAPLELEGLSQAYTSTIQSSLPSKEVFPLEKYSSEPWNLHNLPFENPCLFNYSFSDLSSLTITRLSIGMVFYTHGWTKSSLSTGLLHHHRFGDTVTWYVLPPDESDAFERYLISSYPQYTMEDLNRSNGLPVIVSPSSLIENGFHPIAIDLRPNEFLVVSPNSYHMGFHQGFSSFESVNFATVNWIKDGLLNSSISVLKSMRIPSSVSYEAVIISMVLSKNPCFSSEWLIKCFEDMIANESASKNEIMKLVPNIQALKLESSVPLEIRCSNCKQPCFLSFMQCHEPKKFICLGDCVKEVSLNATSWMLFYRWDVHELSNLAERFVSLIRGPEEWTNRLRSVLSTSPKPQLKVLKSLLVDAEKAMLTTPETVNLRDFVQNANSWIDSVNECLKVASLKRKKDKKPPLFKAHDHWNNTSNLKDSAVLFKVLQTSRSMAFTCQEIENMKQKAFDLLEFRNRLINSFSGPLDKNTCQRLLTEAELLGFTIPELGIIQKYLIQFEWLDMFYSFETTRTTDSDLERLITYGVSAGIPEDNDYMIFAKAMKGRAEIWENQVYDTLSKSNISYDKLSLLRDEAMNLCVNKELFSKVVGILNNAEEIKNKIATLCERSQEKDFALRPSIDEVKEALASAEKLPILSESTVTLQKMYDVVLEWIRRGKRLFGKANAPLEILGQHLDYVEKRNSASLSLNDRPGPPMEPASRETSPDSEGRLTIRKKKGCIFCFCRLPESGVMIECEICHEWYHAKCLKMSKKKLRQDEKFTCPICDYRVEIPRLSNRPKLEDLQSLYKDVKLLPFQPKETETLRKVVDLASKFRQEMQALAHNPFGLTMAEVPLARFYLRKMEGAEILLVDETNLFRQKLHECVPIAPNPPPIIGESKSTRKPRPTKRQRQIMKQVAEGLLPASAIAPPKSSNEKKSSNNVKAVEAETKSKSEKSPKKNGTNISDANNKNESHVSLMKNWKLGSPAFVTLVKEKNSSCLCGEEFSPRDSFIDCTICERRFHYDCVGLNNEIADSVSKFTCPICMEQSGGIYPWQLRPRNGMHPDHISGFSKEVETDPKLGSSGYTLNNSKFDKAAVSKTLSAQDVSRLQKVSCGEHLYFGTDVFTPLGDMATSASMFSLDDSSEKTDAFTENFLNV rik1_schpo Chromatin modification-related protein rik1 rik1 SPCC11E10.08 Silencing protein rik1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MALCVHSFWATAVDTATSCHFISSENCLVLLQALKINIYLCSEVHGLQFFTSIPLFSTVKHIRPYRPPGLDRDYLFVVLNDDTYFSIYWDEDYQKVIVDHPPVRYRVTFPWNRNAKSYCLVDLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSSTPTLVVLHSDGLDCYVTAFLLDLSSKSLGKGIRLFERVKPSMIMPFGKRGLLVFESLFIHCMYRGNFVTINGPCTTYMHWTPLKGQKMHYIVCDTNGYLFGVYSSILGKNKWSLVMERLPIPPFDFITSLNSIHEGLLFIGSKNSESKLINLSTLKDVDSIPNLGPIHDLLVLKNDIEKSFLVCAGTPRNASLIYFQHALKLDILGQTKISGILRAMVLPSYPEHKLFLGFPSETVAFNIKEDFQLELDPSLSTKERTIALSGTNGEFVQVTSTFLCIYDSAKRSRLVYIEKITNAACYQEYSAIVINGTALAIFKKDTEVARKVFESEISCLDFSAQFQIGVGFWSKQVMILTFSDNSSISCAFQTNVPSLPRNIILEGVGVDRNLLLVSSGSGEFKSYVLFKNNLVFSETKHFGTTPVSFRRFTMNIGTYIICNNDCPHMVYGFNGALCYMPLSMPQSYDVCQFRDNSGKDFLISVSLGGLKFLQLNPLPELTPRKVLLEHVPLQAIIFQNKLLLRTLENRYEDYESYKENYHLELVDSYDDNSFRVFSFTENERCEKVLKINESSLLVGTSIIEQDKLVPVNGRLILLEFEKELQSLKVVSSMVLSAAVIDLGVYNDRYIVAFGQQVAIVKLTEERLMIDSRISLGSIVLQLIVEGNEIAIADSIGRFTIMYFDGQKFIVVARYLFGENIVKAALYEGTVYIIATNSGLLKLLRYNKDAKNFNDRFICESVYHLHDKVSKFQNFPITNTNSFLEPKMLFATEIGAIGSIVSLKDKELELEELTRKIRKLKFSYLSSMDYESIEADLISPVPFIDGDLVIDVKRWASSELFRLCRSVEHRESLNSYQKVQALLEEIQSLC 0C76BB59E1215BF1 fas2_schpo Fatty acid synthase subunit alpha fas2 lsd1 p190/210 SPAC4A8.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MRPEVEQELAHTLLLELLAYQFASPVRWIETQDVILSPPVSAERIVEIGPSPTLAGMAKRTLKLKYENMDAALSINREVLCYSKDAREIYYNFEDEVADEPAEAPASTSSTPKVETAAAAAPAATPAPAPAQTSAPAAALPDEPPKALEVLHTLVAQKLKKSIEEVSPQKSIKDLVGGKSTLQNEILGDLQKEFGATPEKPEEVPLDELGAIMQSSFNGSLGKQSSSLISRMISSKMPGGFNNSAVRGYLGNRYGLGPGRLESVLLLALTMEPASRLGSEADAKAWLDSVAQKYAARNGVTLSSPTAEGGSSSGSAAVIDEETFKKLTKNNTMLVTQQLELFARYLNKDLRAGQKAQVAEKVISDTLRAQLDLWNEEHGEFYASGIAPIFSPLKARVYDSDWNWARQDALKMFFDIIFGRLKHVDTEIVARCISVMNRSNPTLLEFMQYHIDHCPAEKGETYQLAKTLGQQLIDNCKSVIDAPPVFKNVNHPTAPSTTIDERGNLNYEEIPRPGVRKLTHYVTEMAKGGKLPTESKNKAKVQNDLARIYRIIKSQNKMSRSSKLQIKQLYGQVLHALSLPLPSSNDEQTPVKETIPFLHIRKKSVDGNWEFNKSLTGTYLDVLESGAKNGITYQDKYALVTGAGAGSIGAQIVEGLLAGGAKVVVTTSRFSRKVTEFYQSLYTRHGSRGSCLIVVPFNQGSKTDVEALIDYIYDEKKGLGWNLDYIVPFAAIPENGREIDGIDSRSEFAHRIMLTNILRLLGAVKSQKASRGMDTRPAQVILPLSPNHGTFGNDGLYSESKLGLETLFNRWYSESWANYLTICGAVIGWTRGTGLMAPNNIVSQGIEKYGVRTFSQSEMAFNILGLMSQKVVDLCQSEPIYANLNGGLELLPDLKDLSTRLRTELLETAEIRRAVAAETAFDHSITNGPDSEAVFQKTAIQPRANLKFNFPKLKPYEALSHLSDLRGMVDLEKVPVVTGFSEVGPWGNSRTRWDMECYGEFSLEGCVEIAWIMGLIKNFNGKGKDGKPYSGWVDTKTGEPVDDKDVKAKYEKYILEHCGIRIIEAELFHGYNPEKKELLQEVVIDHDLEPFEASKEAAHEFKLRHGDQVEIFEIPDSTEWSVRFKRGTSMLIPKALRFDRFVAGQIPLGWDPKRYGIPDDIISQVDPTTLYVLVSTVEALVASGITDPYECYKYIHVSELGNTVGSGIGGMSALRGMYKDRWTDKPVQKDILQESFINTANAWINMLLLSASGPIKTPVGACATAVESVDAAVDLITSGKARICISGGYDDFSEEGSYEFANMGATSNAAKETERGRTPQEMSRPATSTRDGFMESQGAGVQIIMQAKLAIEMGVPIHGIVGYVSTAMDKQGRSVPAPGQGILTGAREIATKTPLPIVDLKFRSRQLQRRRSQIGEWAEREYLYLEEELDAMKVQNPDLDLEAYRIERINVIKEEVVRQEKEALNTFGNEFWKRDPTIAPIRGALAVWGLTIDDLGVASFHGTSTKANEKNECDVIDSQLTHLGRSKGNAVYGVFQKYLTGHSKGGAGAWMLNGALQILRSGFVPGNRNADNIDEYLARFDRVMFPSEGIQTDGIKAASVTAFGFGQVGGQVIVIHPDYIYGVIDEATYNAYKAKTAARYKASYRYTHDALVYNNLVRAKDSPPYTKEQEKAVYLNPLARASKSKAGTWTFPATLPAESDISKTNETTRTLQSLTTSLTNSNENVGVDVELVSAISIDNETFIERNFTDTERKYCFAAPNPQASFAGRWSAKEAVFKSLGISGKGAAAPLKDIEIISSESGAPEVVLHGEAAKAATTAGVKSVSVSISHDDNQSVSVALAHK set1_schpo Histone-lysine N-methyltransferase, H3 lysine-4 specific SPCC306.04c set1 kmt2 Set1 complex component set1 COMPASS component set1 SET domain-containing protein 1 Spset1 Lysine N-methyltransferase 2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDFNTSTRSKSQPVQRNNYKVLYDPELGIKENLGRKIIYRFNGVSKPPLVVRDPRLKNPIYARGIPKSGRPFLKSLQTINYDYNENSLGPEPPTQVFVSNISPLVTSEQLRYHFKSFGEVFDLDLKLNPYTGTSLGLCCISFDKRSSISVAAHSAKIAVQQANGLRFSGKPLSVVLDRDGSLCEEAFKKALNAVEKQFQEETLQKQRFEREDESSRQKLSAAMNEDIPPWRQPSKNSQTLSNGDLQHSKVQNVDQKSGFLTSSETDVPKNINDYIYLLIDDRFVPPDRVYYTDIKHHFRKFLYEKIYMNKDGFYITFNNYREASNCYRALDRTYVQNCRIKLKFHDIPSRTKEDGKKSAVRRVVLPPEEAYAEATSVVLRDLEAALLRDVKSKIIGPAIFKYLHSMPKPSVKEELQENLLVSSTSVPDVPLKIESTVGKLPSLPKFKKRVDSSKMNLSAGSKTKSKLQRRRRRRHEARPLHYQLNQMYNSSASEAESDQELLLSSGDERVERGKIGSIKSVKSDEATPVFSDTSDENDKFHRFRTKSKISKKKYEKMEVDYTSSSETESDASILSPSAAIPKSGSAIKDELISPKKEIDEVLALAPKWRINEFDETGSVYYGALPYNYPEDDVLLDLDGLQYLVKNDEDYSYLQEALKDEPLMDINDPNFWAYERKSCKFKNGDVKYGDTAILPEPKGYFRSNTSGSAKSEGYYIIPTTEKSLYLPLRNRSTIDTISHSTSRITSRMNRVNNRRLAAGVEKSQLPAEADLLRFNALKARKKQLHFGPSRIHTLGLFAMENIDKNDMVIEYIGEIIRQRVADNREKNYVREGIGDSYLFRIDEDVIVDATKKGNIARFINHSCAPNCIARIIRVEGKRKIVIYADRDIMHGEELTYDYKFPEEADKIPCLCGAPTCRGYLN cul4_schpo Cullin-4 pcu4 cul4 SPAC3A11.08 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPPEAKRIVVKGFDPRKSRQRQETYYVTMIDRLNMALQVVMAGLGLKTGYQELYSGVENLTRADQASRCFNILQHHMSSGIQLLKDSAESFIQLEGTETDTNACTVVVGCWNKWLERVEIVQNIFYYMDKTFLSHHPDYPTIEELSLSLFREKLMAVKNIQIPFLNSLLQSFENLHSSKSTDHAYLQDAMLMLHRTEMYSSVFVPMYLVMLSRFYDTESSQKIQELPLEEYLEYAMSSLEREDAYVEKFDIVRDKKSIRETVQRCLITSHLDTLTKGISQFIEKRDAHSCKLLYALLQFNHETEYLIQPWSDCLVDVGFKLVNDESKDDTLVQELLSFHKFLQVVVDESFLHDETLSYAMRKAFETFINGAKGSQREAPARLIAKYIDYLLRVGEQASGGKPLKEVFSEILDLFRYIASKDIFEAYYKLDIAKRLLLNKSASAQNELMLLDMLKKTCGSQFTHSLEGMFRDVNISKEFTSSFRHSKAAHNLHRDLYVNVLSQAYWPSYPESHIRLPDDMQQDLDCFEKFYLSKQVGKKISWYASLGHCIVKARFPLGNKELSISLFQACVLLQFNNCLGGEGISYQDLKKSTELSDIDLTRTLQSLSCARIRPLVMVPKSKKPSPDTMFYVNEKFTDKLYRVKINQIYLKEERQENSDVQEQVVRDRQFELQASIVRVMKQKEKMKHDDLVQYVINNVKDRGIPLVSDVKTAIEKLLEKEYLEREDNDIYTYVT h33_schpo Histone H3.3 hht3 SPBC1105.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MARTKQTARKSTGGKAPRKQLASKAARKAAPATGGVKKPHRYRPPTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQEAVEAYLVSLFEDTNLCAIHGKRVTIQPKDMQLARRLRGERS h31_schpo Histone H3.1/H3.2 hht1 hht2 SPAC1834.04 SPBC8D2.04 pi060 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MARTKQTARKSTGGKAPRKQLASKAARKAAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQEAVEAYLVSLFEDTNLCAIHGKRVTIQPKDMQLARRLRGERS raf1-lid2-1 Interaction between raf1 (clr8) and lid2 776930 n/a 776933 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 776934 unspecified role unspecified role prey prey c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 1D rik1-lid2-1 Interaction between rik1 and lid2 776930 n/a 776935 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 776934 unspecified role unspecified role prey prey c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp. Fig. 1 lsd1-lid2-1 Interaction between lsd1 and lid2 776930 n/a 776934 unspecified role unspecified role prey prey c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 776936 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6E set1-lid2-1 Interaction between set1 and lid2 776930 n/a 776934 unspecified role unspecified role prey prey c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false jmjc region required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range false n/a 776937 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6G The boundaries of the JmjC domain are not given in the paper so these have been inferred from the UniProt record cul4-lid2-1 Interaction between cul4 and lid2 776930 n/a 776934 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 776938 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp. Fig. 6 set1-lid2-2 Interaction between set1 and lid2 776930 n/a 776937 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 776934 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp. Fig. 6 hht3-lid3-1 Lid3 interacts with HHT3 peptide by pulldown 776931 n/a 776939 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false lys-10 xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) certain certain sequence position certain certain certain sequence position certain false c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 776934 unspecified role unspecified role prey prey phd2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false direct interaction direct interaction false false false Histone numbering does not take account of the N-terminal cleaved methionine Coordinates of the PHD2 domain are not supplied in the paper and have been inferred from the UniProtKB entry. Fig. 7D hht3-lid3-2 Lid3 interacts with HHT3 peptide by pulldown 776931 n/a 776934 unspecified role unspecified role prey prey phd2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false n/a 776940 unspecified role unspecified role bait bait c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lys-10 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Histone numbering does not take account of the N-terminal cleaved methionine Coordinates of the PHD2 domain are not supplied in the paper and have been inferred from the UniProtKB entry. Fig. 7D raf1-5 Interactors of raf1 (clr8) 776932 n/a 776934 unspecified role unspecified role prey prey n/a 776933 unspecified role unspecified role bait bait n-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 776938 unspecified role unspecified role prey prey association association false false false Fig. 1C