IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ taverna-2006-2 Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Taverna SD., Ilin S., Rogers RS., Tanny JC., Lavender H., Li H., Baker L., Boyle J., Blair LP., Chait BT., Patel DJ., Aitchison JD., Tackett AJ., Allis CD. Chromatin - Epigenetic interactions resulting in chromatin modulation Tagged YNG1 cells, grown in isotopically light media, and wild-type (untagged) cells, grown in heavy isotopic (d4-lysine) media, were mixed in a 1:1 ratio by cell weight and colysed. Protein A (PrA) component of the TAP tag was utilized for purification using IgG-coated Dynabeads. Analysis allowed identification of proteins that were either stably associated (peptides yielding an exclusively light labeled signature) or nonstably associated (1:1 ratio of light to heavy labeled peptides) with tagged Yng1. Proteins identified as containing near 100% h4-lysine (isotopically light) were regarded as true protein complex components, while protein identifications containing 50% h4-lysine (and therefore 50% d4-lysine) aas contaminants (I-DIRT analysis). ajtackett@uams.edu, alliscd@rockefeller.edu Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation taverna-2006-1 Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down sequence tag sequence tag identification sequence tag Taverna SD., Ilin S., Rogers RS., Tanny JC., Lavender H., Li H., Baker L., Boyle J., Blair LP., Chait BT., Patel DJ., Aitchison JD., Tackett AJ., Allis CD. Chromatin - Epigenetic interactions resulting in chromatin modulation Tagged YNG1 cells, grown in isotopically light media, and wild-type (untagged) cells, grown in heavy isotopic (d4-lysine) media, were mixed in a 1:1 ratio by cell weight and colysed. Protein A (PrA) component of the TAP tag was utilized for purification using IgG-coated Dynabeads. Analysis allowed identification of proteins that were either stably associated (peptides yielding an exclusively light labeled signature) or nonstably associated (1:1 ratio of light to heavy labeled peptides) with tagged Yng1. Proteins identified as containing near 100% h4-lysine (isotopically light) were regarded as true protein complex components, while protein identifications containing 50% h4-lysine (and therefore 50% d4-lysine) aas contaminants (I-DIRT analysis). ajtackett@uams.edu, alliscd@rockefeller.edu Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation taverna-2006-3 Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Taverna SD., Ilin S., Rogers RS., Tanny JC., Lavender H., Li H., Baker L., Boyle J., Blair LP., Chait BT., Patel DJ., Aitchison JD., Tackett AJ., Allis CD. Chromatin - Epigenetic interactions resulting in chromatin modulation Tagged YNG1 cells, grown in isotopically light media, and wild-type (untagged) cells, grown in heavy isotopic (d4-lysine) media, were mixed in a 1:1 ratio by cell weight and colysed. Protein A (PrA) component of the TAP tag was utilized for purification using IgG-coated Dynabeads. Analysis allowed identification of proteins that were either stably associated (peptides yielding an exclusively light labeled signature) or nonstably associated (1:1 ratio of light to heavy labeled peptides) with tagged Yng1. Proteins identified as containing near 100% h4-lysine (isotopically light) were regarded as true protein complex components, while protein identifications containing 50% h4-lysine (and therefore 50% d4-lysine) aas contaminants (I-DIRT analysis). ajtackett@uams.edu, alliscd@rockefeller.edu Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation taverna-2006-4 Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Taverna SD., Ilin S., Rogers RS., Tanny JC., Lavender H., Li H., Baker L., Boyle J., Blair LP., Chait BT., Patel DJ., Aitchison JD., Tackett AJ., Allis CD. Chromatin - Epigenetic interactions resulting in chromatin modulation Tagged YNG1 cells, grown in isotopically light media, and wild-type (untagged) cells, grown in heavy isotopic (d4-lysine) media, were mixed in a 1:1 ratio by cell weight and colysed. Protein A (PrA) component of the TAP tag was utilized for purification using IgG-coated Dynabeads. Analysis allowed identification of proteins that were either stably associated (peptides yielding an exclusively light labeled signature) or nonstably associated (1:1 ratio of light to heavy labeled peptides) with tagged Yng1. Proteins identified as containing near 100% h4-lysine (isotopically light) were regarded as true protein complex components, while protein identifications containing 50% h4-lysine (and therefore 50% d4-lysine) aas contaminants (I-DIRT analysis). ajtackett@uams.edu, alliscd@rockefeller.edu Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation taverna-2006-5 Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Taverna SD., Ilin S., Rogers RS., Tanny JC., Lavender H., Li H., Baker L., Boyle J., Blair LP., Chait BT., Patel DJ., Aitchison JD., Tackett AJ., Allis CD. Chromatin - Epigenetic interactions resulting in chromatin modulation Tagged YNG1 cells, grown in isotopically light media, and wild-type (untagged) cells, grown in heavy isotopic (d4-lysine) media, were mixed in a 1:1 ratio by cell weight and colysed. Protein A (PrA) component of the TAP tag was utilized for purification using IgG-coated Dynabeads. Analysis allowed identification of proteins that were either stably associated (peptides yielding an exclusively light labeled signature) or nonstably associated (1:1 ratio of light to heavy labeled peptides) with tagged Yng1. Proteins identified as containing near 100% h4-lysine (isotopically light) were regarded as true protein complex components, while protein identifications containing 50% h4-lysine (and therefore 50% d4-lysine) aas contaminants (I-DIRT analysis). ajtackett@uams.edu, alliscd@rockefeller.edu Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation taverna-2006-6 Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. in vitro In vitro acetylase assay acetylase assay predetermined predetermined participant predetermined Taverna SD., Ilin S., Rogers RS., Tanny JC., Lavender H., Li H., Baker L., Boyle J., Blair LP., Chait BT., Patel DJ., Aitchison JD., Tackett AJ., Allis CD. Chromatin - Epigenetic interactions resulting in chromatin modulation Tagged YNG1 cells, grown in isotopically light media, and wild-type (untagged) cells, grown in heavy isotopic (d4-lysine) media, were mixed in a 1:1 ratio by cell weight and colysed. Protein A (PrA) component of the TAP tag was utilized for purification using IgG-coated Dynabeads. Analysis allowed identification of proteins that were either stably associated (peptides yielding an exclusively light labeled signature) or nonstably associated (1:1 ratio of light to heavy labeled peptides) with tagged Yng1. Proteins identified as containing near 100% h4-lysine (isotopically light) were regarded as true protein complex components, while protein identifications containing 50% h4-lysine (and therefore 50% d4-lysine) aas contaminants (I-DIRT analysis). ajtackett@uams.edu, alliscd@rockefeller.edu Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation yng1_yeast Protein YNG1 ING1 homolog 1 YNG1 YOR29-15 YOR064C protein protein yeast Saccharomyces cerevisiae Baker's yeast MEHLANENSDSDIRYSFLSTLDHLPCELIRSLRLMQTIDLFKNEEDEPGMERACRDLLLVATYINDLVDDQIHFLKQHKKELEIQKSVTKNFNSSLENIKSKLTLEEPGAYKEPKLLLKINLKKAKSRERKESITSPTIGINQGDVTEGNNNQEEVYCFCRNVSYGPMVACDNPACPFEWFHYGCVGLKQAPKGKWYCSKDCKEIANQRSKSKRQKRRK 5232A71839E2FDA4 h3_yeast Histone H3 SIN2 YNL031C N2749 HHT1 YBR010W YBR0201 HHT2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTIQKKDIKLARRLRGERS A6115FEB480AC67A eaf6_yeast Chromatin modification-related protein EAF6 YJR082C J1854 ESA1-associated factor 6 EAF6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDELKSYEALKAELKKSLQDRREQEDTFDNLQQEIYDKETEYFSHNSNNNHSGHGGAHGSKSHYSGNIIKGFDTFSKSHHSHADSAFNNNDRIFSLSSATYVKQQHGQSQND nto1_yeast NuA3 HAT complex component NTO1 YPR031W NTO1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNRGSLDDGPKLREEKHFQDFYPDLNADTLLPFIVPLVETKDNSTDTDSDDISNRNNREIGSVKSVQTKELIFKGRVTTEPLVLKKNEVEFQKCKITTNELKGKKNPYCVRFNESFISRYYHINKVRNRKSYKQQQKEFDGVEAPYFTKFSSKEAPNITISTSTKSAIQKFASISPNLVNFKPQYDMDEQDELYLHYLNKRYFKDQMSHEIFEILMTTLETEWFHIEKHIPSTNSLIARHNILRDCKNYELYGSDDGTGLSMDQACAVCLGTDSDNLNTIVFCDGCDIAVHQECYGIIFIPEGKWLCRRCMISKNNFATCLMCPSHTGAFKQTDTGSWVHNICALWLPELYFSNLHYMEPIEGVQNVSVSRWKLNCYICKKKMGACIQCFQRNCFTAYHVTCARRAGLYMSKGKCTIQELASNQFSQKYSVESFCHKHAPRGWQTSIEGINKARKYFSLLSTLQTETPQHNEANDRTNSKFNKTIWKTPNQTPVAPHVFAEILQKVVDFFGLANPPAGAFDICKYWSMKRELTGGTPLTACFENNSLGSLTEEQVQTRIDFANDQLEDLYRLKELTTLVKKRTQASNSLSRSRKKVFDIVKSPQKYLLKINVLDIFIKSEQFKALERLVTEPKLLVILEKCKHCDFDTVQIFKEEIMHFFEVLETLPGASRILQTVSSKAKEQVTNLIGLIEHVDIKKLLSRDFIINDDKIEERPWSGPVIMEEEGLSDAEELSAGEHRMLKLILNSG 7D6796553BCFA06D taf14_yeast Transcription initiation factor TFIID subunit 14 ANC1 CST10 YPL129W TAF14 SWP29 TAF30 TFG3 TBP-associated factor 14 TBP-associated factor 30 kDa Transcription initiation factor TFIIF 30 kDa subunit Transcription factor G 30 kDa subunit SWI/SNF chromatin-remodeling complex subunit TAF14 SWI/SNF complex subunit TAF14 SWI/SNF complex 29 kDa subunit Actin non-complementing mutant 1 Chromosome stability protein 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTIGKRRTTTNTTAEPKAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGVVQMVTDNKTPEMNVTNNVEEGEFIIDLYSLPEGLLKSLWDYVKKNTE 60EA5EC2474B1B9C sas3_yeast Histone acetyltransferase SAS3 SAS3 YBL052C YBL0515 YBL0507 Something about silencing protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLTANDESPKPKKNALLKNLEIDDLIHSQFVRSDTNGHRTTRRLFNSDASISHRIRGSVRSDKGLNKIKKGLISQQSKLASENSSQNIVNRDNKMGAVSFPIIEPNIEVSEELKVRIKYDSIKFFNFERLISKSSVIAPLVNKNITSSGPLIGFQRRVNRLKQTWDLATENMEYPYSSDNTPFRDNDSWQWYVPYGGTIKKMKDFSTKRTLPTWEDKIKFLTFLENSKSATYINGNVSLCNHNETDQENEDRKKRKGKVPRIKNKVWFSQIEYIVLRNYEIKPWYTSPFPEHINQNKMVFICEFCLKYMTSRYTFYRHQLKCLTFKPPGNEIYRDGKLSVWEIDGRENVLYCQNLCLLAKCFINSKTLYYDVEPFIFYILTEREDTENHPYQNAAKFHFVGYFSKEKFNSNDYNLSCILTLPIYQRKGYGQFLMEFSYLLSRKESKFGTPEKPLSDLGLLTYRTFWKIKCAEVLLKLRDSARRRSNNKNEDTFQQVSLNDIAKLTGMIPTDVVFGLEQLQVLYRHKTRSLSSLDDFNYIIKIDSWNRIENIYKTWSSKNYPRVKYDKLLWEPIILGPSFGINGMMNLEPTALADEALTNETMAPVISNNTHIENYNNSRAHNKRRRRRRRSSEHKTSKLHVNNIIEPEVPATDFFEDTVSSLTEYMCDYKNTNNDRLIYQAEKRVLESIHDRKGIPRSKFSTETHWELCFTIKNSETPLGNHAARRNDTGISSLEQDEVENDVDTELYVGENAKEDEDEDEDFTLDDDIEDEQISEENDEEEDTYEEDSDDDEDGKRKGQEQDENDIESHIRKERVRKRRKITLIEDDEE h3-yng1-1 Interaction of Yng1 with H3 peptide demonstrated by pulldown 882307 n/a 882313 unspecified role unspecified role prey prey Q08465:p.Trp180Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882314 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false lys-5 (required) n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 2C Interaction observed in nto1 and sas3 -/- cells h3-yng1-2 Interaction of Yng1. Sas3, Nto1, Taf14 (Taf30) and Eaf6 with H3 peptide demonstrated by pulldown 882307 n/a 882315 unspecified role unspecified role prey prey region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 882316 unspecified role unspecified role prey prey region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882317 unspecified role unspecified role prey prey region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882314 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false lys-5 (required) n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 882313 unspecified role unspecified role prey prey region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882318 unspecified role unspecified role prey prey region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Fig. 2A Although this experiment was done as a series of bait/prey pairs, subsequent knockout experiments showed only Yng1 interacts directly with the peptide so the data has been represented as a one bait many prey, to show a truer representation. Low levels of binding were observed with the dimethylated peptide. yng1-eaf6-1 Interactors of Yng1 882308 n/a 882315 unspecified role unspecified role prey prey n/a 882317 unspecified role unspecified role prey prey n/a 882316 unspecified role unspecified role prey prey n/a 882318 unspecified role unspecified role prey prey n/a 882313 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 1B h3-yng1-3 Interaction of Yng1 with H3 peptide demonstrated by pulldown 882309 n/a 882314 unspecified role unspecified role prey prey lys-5 (required) n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882313 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q08465:p.Trp180Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false phd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3A A lower level of binding was observed to dimethylated Histone H3. h3-yng1-4 Interaction of Yng1 with H3 peptide demonstrated by nmr 882310 n/a 882314 unspecified role unspecified role neutral component neutral component lys-5 (required) n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 882313 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 1H isotope label isotope label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 882362 882359 direct interaction direct interaction false false false Figs. 3B & 4 Supp. Fig. 2 A lower level of binding was observed to dimethylated Histone H3. h3-yng1-5 Interaction of Yng1 with H3 peptide demonstrated by fluorescence polarisation 882311 n/a 882313 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 1H isotope label isotope label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 882314 unspecified role unspecified role neutral component neutral component lys-5 (required) n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Figs. 3C, 3D KD H3K5(Me)3=9.1E-6M, KD H3K5(Me)2=2.1E-5M, KD H3K5(Me)1=5.1E-5M Temp= room temperature, pH 7.5. yng1-eaf6-2 Acetylation of H3 by the NuA3 complex 882312 n/a 882314 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false lys-5 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 882313 unspecified role unspecified role ancillary ancillary region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882318 enzyme enzyme neutral component neutral component yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882317 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882316 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 882315 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast acetylation acetylation reaction acetylation false false false Fig. 5