IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ liu-2004-1 Leukemia/lymphoma-related factor, a POZ domain-containing transcriptional repressor, interacts with histone deacetylase-1 and inhibits cartilage oligomeric matrix protein gene expression and chondrogenesis. mouse-c3h10t1_2 Mouse embryonic fibroblast C3H10T1_2 c3h10t1_2 Mouse mesenchymal stem cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu CJ., Prazak L., Fajardo M., Yu S., Tyagi N., Di Cesare PE. Chromatin - Epigenetic interactions resulting in chromatin modulation PEDiCesare@aol.com J. Biol. Chem. (0021-9258) 2004 liu-2004-2 Leukemia/lymphoma-related factor, a POZ domain-containing transcriptional repressor, interacts with histone deacetylase-1 and inhibits cartilage oligomeric matrix protein gene expression and chondrogenesis. in vitro In vitro pull down pull down polyclonal western polyclonal antibody western blot polyclonal western Liu CJ., Prazak L., Fajardo M., Yu S., Tyagi N., Di Cesare PE. Chromatin - Epigenetic interactions resulting in chromatin modulation PEDiCesare@aol.com J. Biol. Chem. (0021-9258) 2004 hdac1_mouse Histone deacetylase 1 Hdac1 protein protein mouse Mus musculus Mouse MAQTQGTKRKVCYYYDGDVGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKANAEEMTKYHSDDYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVASAVKLNKQQTDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNYPLRDGIDDESYEAIFKPVMSKVMEMFQPSAVVLQCGSDSLSGDRLGCFNLTIKGHAKCVEFVKSFNLPMLMLGGGGYTIRNVARCWTYETAVALDTEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEEDPDKRISICSSDKRIACEEEFSDSDEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA zbt7a_mouse Zinc finger and BTB domain-containing protein 7A Leukemia/lymphoma-related factor POZ and Krueppel erythroid myeloid ontogenic factor Zbtb7a Lrf Zbtb7 protein protein mouse Mus musculus Mouse MAGGVDGPIGIPFPDHSSDILSGLNEQRTQGLLCDVVILVEGREFPTHRSVLAACSQYFKKLFTSGAVVDQQNVYEIDFVSAEALTALMDFAYTATLTVSTANVGDILSAARLLEIPAVSHVCADLLERQILAADDVGDASQPDGAGPTDQRNLLRAKEYLEFFRSNPMNSLPPTAFPWSGFGAPDDDLDATKEAVAAAVAAVAAGDCNGLDFYGPGPPADRPPAGDGDEGDSTPGLWPERDEDAPPGGLFPPPTAPPATTQNGHYGRAGAGTGEEEAAALSEAAPEPGDSPGFLSGAAEGEDGDAADVDGLAASTLLQQMMSSVGRAGDSDEESRTDDKGVMDYYLKYFSGAHEGDVYPAWSQKGEKKIRAKAFQKCPICEKVIQGAGKLPRHIRTHTGEKPYECNICKVRFTRQDKLKVHMRKHTGEKPYLCQQCGAAFAHNYDLKNHMRVHTGLRPYQCDSCCKTFVRSDHLHRHLKKDGCNGVPSRRGRKPRVRGVPPDVPAGAGAPPGLPDAPRNGQEKHFKDEEEDEEEASPDGSGRLNVAGSGGDDGAGGPAVATAEGNFAT zbtb7-hdac1-1 Interaction between ZBTB7 and HDAC1 detected by CoIP. 185103 N/A 185105 unspecified role unspecified role prey prey N/A 185106 unspecified role unspecified role bait bait physical association physical association false false false chondrosarcoma FIG. 10. LRF associates with HDAC1 A, LRF associates with HDAC1 in vivo (co-immunoprecipitation assay). Cell extracts were incubated with anti-LRF (lane 2) or anti-HDAC1 (lane 3) antibody or with control IgG (lane 4), followed by protein A/G-agarose. The immunoprecipitated (IP) protein complex and cell extracts (lane 1, which provides a positive control) were examined by immunoblotting with anti-HDAC1 antibody. zbtb7-hdac1-2 Interaction between ZBTB7 and HDAC1 detected by GST pull down 185104 N/A 185106 unspecified role unspecified role prey prey in vitro In vitro N/A 185105 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false chondrosarcoma FIG. 10. LRF associates with HDAC1 B, LRF associates with HDAC1 in vitro (GST pull-down assay). Purified GST (lane 2), GST-HDAC1-(51-482) (lane 3), and GST-HDAC1-(1-432) (lane 4) immobilized on glutathione-Sepharose beads were incubated with in vitro translated (IVT) LRF (lane 1). Bound proteins were examined by immunoblotting with anti-LRF antibodies.