IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ vary-2003-4 Yeast Isw1p forms two separable complexes in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Vary JC., Gangaraju VK., Qin J., Landel CC., Kooperberg C., Bartholomew B., Tsukiyama T. Chromatin - Epigenetic interactions resulting in chromatin modulation ttsukiya@fhcrc.org Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation vary-2003-2 Yeast Isw1p forms two separable complexes in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip weight by staining molecular weight estimation by staining weight by staining Vary JC., Gangaraju VK., Qin J., Landel CC., Kooperberg C., Bartholomew B., Tsukiyama T. Chromatin - Epigenetic interactions resulting in chromatin modulation ttsukiya@fhcrc.org Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation vary-2003-3 Yeast Isw1p forms two separable complexes in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Vary JC., Gangaraju VK., Qin J., Landel CC., Kooperberg C., Bartholomew B., Tsukiyama T. Chromatin - Epigenetic interactions resulting in chromatin modulation Complexes separated on a Superose 6 10/30 column ttsukiya@fhcrc.org Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation vary-2003-5 Yeast Isw1p forms two separable complexes in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap weight silver stain molecular weight estimation by silver staining weight silver stain Vary JC., Gangaraju VK., Qin J., Landel CC., Kooperberg C., Bartholomew B., Tsukiyama T. Chromatin - Epigenetic interactions resulting in chromatin modulation Identity of prey proteins further confirmed by mass spectrometry but this data was not shown. ttsukiya@fhcrc.org Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation vary-2003-6 Yeast Isw1p forms two separable complexes in vivo. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Vary JC., Gangaraju VK., Qin J., Landel CC., Kooperberg C., Bartholomew B., Tsukiyama T. Chromatin - Epigenetic interactions resulting in chromatin modulation ATPase assays of purified yeast ISWI complexes were performed using radio-labelled ATP in the presence of magnesium. Reaction mixture was spotted onto thin layer chromatography plates. ATP and free phosphate were separated, localized by autoradiograph, and quantified by scintillation counting. ttsukiya@fhcrc.org Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation vary-2003-1 Yeast Isw1p forms two separable complexes in vivo. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom ms participant Identification by mass spectrometry ms participant Vary JC., Gangaraju VK., Qin J., Landel CC., Kooperberg C., Bartholomew B., Tsukiyama T. Chromatin - Epigenetic interactions resulting in chromatin modulation ttsukiya@fhcrc.org Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation ioc2_yeast ISWI one complex protein 2 IOC2 YLR095C protein protein yeast Saccharomyces cerevisiae Baker's yeast MRTKRTRGTRNVGASMPAGAANADSEDWKEYVSEDIIAQLNKHQLPYSEILDEKIADLANHWHFQYVMAWLSNVCESYTTTTFNTDQYGGSSTKCLWKNIKFDEGVFVTDVFSKIDGKDSNYYNDEVDVDEGSQNLYDRIRLQLLHQLAGNKSGQLKDWNVIVNHHLQNSSAYSDLVTDLPFLELEIARQFDIIYSIIKLIEMKNMIFKNYLANNLHLFTFSEVILDDDNSGGEEMKSLFALPNVGVLVKKTIHRVKEGSSSQVSQTLNIPIKLQNCTIKESDPDIPDSVELIHLEYSHDIDAYLQSITIDYDVITTNWGSMLEYWSENKSSKAIDEFITNLIPVYAEHRLYSAKLLANREKERAIAELMTRRKRSSRLVAKEEENKKKDLESEWFEKLDEREQFIRHRNKLVSKEIKKIKDLLWNQLWQLYDQDYRDEKLTRRNELKDRSGSGTPFFETSLGREEDNPLNEIDIGVLDHGPNFQSSIIPVEPPIPGTVGPLQTGDVPELPSDFCITKEELDELANYGIFTPQQEPDNQDSVFQCPGEPELAPMIITEDTETDLFNNRPLICCDHCYRWQHWECQPPKIIELISSTTKSPQHTLSQRDFGVIIMGNSHGNRRSSRRPQSTLEPSTKSSRPTDKRKPLSECSTFICAWCIRDLELELRNIFVPELKIIRAKQRKQQEDRERRKKMKEEKKRLEELAKKRELTESVSPPVFNNAFANMSSSTTPSIAAYEKTNPAINPAPNVNAAHPIITYSQQTGSKTVPQAPQAPQTSQASIQPQQQQQQQQQQQPLHPKEQNFHFQFPPTN 632AD02AA8229D87 isw1_yeast ISWI chromatin-remodeling complex ATPase ISW1 YBR1633 YBR245C ISW1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAYMLAIANFHFFKFYTRMRKKHENNSCNEKDKDENLFKIILAIFLQEKKKYDCISSGSIMTASEEYLENLKPFQVGLPPHDPESNKKRYLLKDANGKKFDLEGTTKRFEHLLSLSGLFKHFIESKAAKDPKFRQVLDVLEENKANGKGKGKHQDVRRRKTEHEEDAELLKEEDSDDDESIEFQFRESPAYVNGQLRPYQIQGVNWLVSLHKNKIAGILADEMGLGKTLQTISFLGYLRYIEKIPGPFLVIAPKSTLNNWLREINRWTPDVNAFILQGDKEERAELIQKKLLGCDFDVVIASYEIIIREKSPLKKINWEYIIIDEAHRIKNEESMLSQVLREFTSRNRLLITGTPLQNNLHELWALLNFLLPDIFSDAQDFDDWFSSESTEEDQDKIVKQLHTVLQPFLLRRIKSDVETSLLPKKELNLYVGMSSMQKKWYKKILEKDLDAVNGSNGSKESKTRLLNIMMQLRKCCNHPYLFDGAEPGPPYTTDEHLVYNAAKLQVLDKLLKKLKEEGSRVLIFSQMSRLLDILEDYCYFRNYEYCRIDGSTAHEDRIQAIDDYNAPDSKKFVFLLTTRAGGLGINLTSADVVVLYDSDWNPQADLQAMDRAHRIGQKKQVKVFRLVTDNSVEEKILERATQKLRLDQLVIQQNRTSLKKKENKADSKDALLSMIQHGAADVFKSGTSTGSAGTPEPGSGEKGDDIDLDELLLKSENKTKSLNAKYETLGLDDLQKFNQDSAYEWNGQDFKKKIQRDIISPLLLNPTKRERKENYSIDNYYKDVLNTGRSSTPSHPRMPKPHVFHSHQLQPPQLKVLYEKERMWTAKKTGYVPTMDDVKAAYGDISDEEEKKQKLELLKLSVNNSQPLTEEEEKMKADWESEGFTNWNKLEFRKFITVSGKYGRNSIQAIARELAPGKTLEEVRAYAKAFWSNIERIEDYEKYLKIIENEEEKIKRVKMQQEALRRKLSEYKNPFFDLKLKHPPSSNNKRTYSEEEDRFILLMLFKYGLDRDDVYELVRDEIRDCPLFELDFYFRSRTPVELARRGNTLLQCLEKEFNAGIVLDDATKDRMKKEDENGKRIREEFADQTANEKENVDGVESKKAKIEDTSNVGTEQLVAEKIPENETTH 2CF86BAF553E6695 ioc3_yeast ISWI one complex protein 3 YFR013W IOC3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSPSNSIQNLQQEAQGSSSAQLADHDHDRVSMAMPLQTDQSVSVSQSSDNLRRSRRVPKPRTSIYDEYEEELKERANKPKRKRPAPPKKKAPSTQNSKSNDKVEKKKTTSIAKDGKPTLKTNDKKVAPKPKPAHEQVEPALIPSNWTSVIPLLTSDFKNQYSVISRLKNPNMKPVPYAGDIIKLMAFINKFSSFFHSDLQNLSFQDFEVGLDLYPGDPNGSAAGIVKGPEDTSLLLYPDFMAIKDIVYCQDKMNLLFLSLLDLTFTENFDGKSAKKKGPLTTWENLKSSSKKVFSNPLYRLRLVAREWGYPREWRQQLPSDQDISKPKTALFEQDEQTPVVDPSHPEILTPNIYTWNANEPLPLESNPLYNREMDKNGILALKPMDRVVLLRALTDWCASHSSAIHDEIYKLTHGKKDPVFGIQTQQVPRYTIEGVDNTINQFKKLCSLIQSRYEIRSKKKHFVKQLKEGKKPDLSRKLEILKEIKAELKNAVKSEKDELLFSLYDKWVPLFEGELPDQPLANPFSERLYKLRLQEFFLGRVPHIGDFYMPRLHSYGDSLEMSTFTDLRNLQALLSKFKNNEYNAFTLFENDGQSMSAQFKLFYHDTPSLAHDVARGRNTSGKVYWYELCHDSATLLEFLEFLDYKIVKPQDEKKEGNEKEKEALNNEAHILEQKSTTDNNPSINTNPLPKDAKYNTARKKLQILKEFLSDYYFILRQFEQMKVQFADMKPGKRQLRRIQRQTVNYNTEYDSEEYVDDEEDDEADIYDDNDNDSSFDDGRVKRQRT 1919A8A1F58B5340 ioc4_yeast ISWI one complex protein 4 YM9532.09 YMR044W IOC4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEAIFQPTDIVLAKVKGFSAWPAMIIPNELIPDNILKTKPVSVHKGKSGSDKKANEDIDADMESEARDREQSEEEEDIEDFGESEANPEKFIIYTPVLKFRKNDTLKSTYCVKFFCDDSYIWVKPMDMKILTSEDCRKWLSGKQRKNKKLIPAYEMAMRGKNGIDIWEFVEYGSYGKPDEEEYVEEEEEENEPEKKAIRPTRSSSRQRQKRASETEKSEGGNSNKRKRVTRSTRQQAIDASEEEEEEEEEKVQEAVRKRPQRTKTKKVVVSKTKPNPKTKAKKEKPKPPKPIKYHFEDDEDWSIVGLGPQDLSIEKTMDPIAKKLSQKKNLEKHVEIKLDLEDKLAGINKLLCDVLCSAINQAVSIKDDFEIILDELQIALDTRGSRNEFITIFQSNNSLLLNFRILFNLRKRELNKWDLWDRFQDIFKHIYSYQFIPDTEDWQLEQNMEIEEMDREKPSFSEDVKEEESKVGA atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) isw1-ioc2-4 Interaction between ISW1, IOC2 and IOC3 identified by coimmunoprecipitation 607245 N/A 607251 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607253 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2B isw1-ioc2-5 Interaction between ISW1 and IOC2 identified by coimmunoprecipitation 607245 N/A 607252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607251 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2B isw1-ioc3-3 Interaction between ISW1 and IOC3 identified by coimmunoprecipitation 607245 N/A 607252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607253 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2B isw1-ioc2-2 Interaction between ISW1, IOC2 and IOC4 by coimmunoprecipitation 607246 N/A 607252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607254 unspecified role unspecified role prey prey N/A 607251 unspecified role unspecified role prey prey association association false false false 1 Immunopreciptiation performed in cells not expressing IOC3 (P43596) isw1-ioc3-1 Interaction between ISW1 and IOC3 by coimmunoprecipitation 607246 N/A 607253 unspecified role unspecified role prey prey N/A 607252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 Coimmunoprecipitation performed in both a IOC2 and IOC4 deleted cell line. isw1-ioc2-3 Interaction between ISW1and IOC2 identified by gel filtration 607247 N/A 607252 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607251 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2 isw1-ioc3-2 Interaction between ISW1and IOC3 identified by gel filtration 607247 N/A 607252 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607253 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2 isw1-ioc2-6 Interaction between ISW1, IOC2 and IOC4 identified by TAP 607248 N/A 607252 unspecified role unspecified role prey prey N/A 607251 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607254 unspecified role unspecified role prey prey association association false false false 2C isw1-ioc3-4 Interaction between ISW1 and IOC3 by TAP 607248 N/A 607253 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 607252 unspecified role unspecified role prey prey physical association physical association false false false 2C isw1-ioc2-7 ATPase activity of Isw1b complex 607249 N/A 607252 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 607251 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 607255 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 607254 unspecified role unspecified role ancillary ancillary yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 3A Nucleosomes. isw1-ioc3-5 ATPase activity of IWS1a complex 607249 N/A 607255 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 607252 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 607253 unspecified role unspecified role ancillary ancillary region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction false false false 3A Nucleosomes isw1-ioc2-1 Interaction between ISW1, IOC2, IOC3 and IOC4 by ion exchage chromatography 607250 N/A 607254 unspecified role unspecified role prey prey N/A 607253 unspecified role unspecified role prey prey N/A 607251 unspecified role unspecified role prey prey N/A 607252 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1