UniProt UniProt http://www.uniprot.org lechner-2000-2 PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lechner MS., Levitan I., Dressler GR. Chromatin - Epigenetic interactions resulting in chromatin modulation dressler@umich.edu mouse embryonic cDNA library Nucleic Acids Res. (0305-1048) 2000 lechner-2000-3 PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin. mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Lechner MS., Levitan I., Dressler GR. Chromatin - Epigenetic interactions resulting in chromatin modulation NIH 3T3 cells have been transiently transfected with tagged forms of Pax2 and Paxip1. dressler@umich.edu mouse embryonic cDNA library Nucleic Acids Res. (0305-1048) 2000 lechner-2000-1 PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lechner MS., Levitan I., Dressler GR. Chromatin - Epigenetic interactions resulting in chromatin modulation dressler@umich.edu mouse embryonic cDNA library Nucleic Acids Res. (0305-1048) 2000 lechner-2000-4 PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lechner MS., Levitan I., Dressler GR. Chromatin - Epigenetic interactions resulting in chromatin modulation dressler@umich.edu mouse embryonic cDNA library Nucleic Acids Res. (0305-1048) 2000 lechner-2000-5 PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin. mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Lechner MS., Levitan I., Dressler GR. Chromatin - Epigenetic interactions resulting in chromatin modulation NIH 3T3 cells have been transiently transfected with Pax2. dressler@umich.edu mouse embryonic cDNA library Nucleic Acids Res. (0305-1048) 2000 p32114-1 Paired box protein Pax-2 Pax2 Pax-2 protein protein mouse Mus musculus Mouse MDMHCKADPFSAMHRHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQNPTMFAWEIRDRLLAEGICDNDTVPSVSSINRIIRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTASPPVSSASNDPVGSYSINGILGIPRSNGEKRKREEVEVYTDPAHIRGGGGLHLVWTLRDVSEGSVPNGDSQSGVDSLRKHLRADTFTQQQLEALDRVFERPSYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKSSLSASANPELGSNVSGTQTYPVVTGRDMTSTTLPGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNPYSHPQYTAYNEAWRFSNPALLSSPYYYSAAPRGSAPAAAAYDRH paxi1_mouse PAX-interacting protein 1 Paxip1 Ptip PAX transactivation activation domain-interacting protein protein protein mouse Mus musculus Mouse MSEPAPEVPEELFREVKYYAVGDIDPQVIQLLKAGKAKEVSYNALASHIISEDGDNPEVGEAREVFDLPVVKPSWVTLSVQCGALLPVNGFSPESCQIFFGLTACLSQVSSEDRSALWALVTFHGGSCQLNLNKKCTHLIVPEPKGEKYERAVKRTSIKIVTPDWVLDCVSEKRRKDEAFYHPRLIIYEEEEEEEEEGDNEEQDSQNEGSTEKSSVASSAVASPAEQPCSPKPRAEVSKGELMFDDSSDSSPEKQERSLNWAPAEAPPLNTAQRRLPQGKGPGLINLCANVPPVPGDILPPDMRGNLMAPGQNLQNSERSEILGTWSPAVRTLRNITNNADIQQINRPSNVAHILQSLSAPTKSLEQQVARGQQGHPNASAVLFGQAKGAPETHVLQQHHPPQQPQQQHPALHLQPQIMQLQQQQQQQQQQQQQPQPYPQPPSHQFPQQVHQHQFSQQQLQFPQQPLHPQQQLHRPQQQLQPFQQQHALQQQLHQLQQQQLQHHQLAQLQQQQQQQHNLLQQQQQQQQLQRLQQQQQMQNQAAHLSQASQALQHQVLPQQPLQLSLQPPPQQQQQQQLFGHDPAVEIPEESFLLGCVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCASQVSSMYTQALRERKRCVTAHWLNTVLKKKKLMPPHRALHFPVAFPPGGKPCSQHIISVTGFVDNDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPSGLKYEKAKEWRIPCVNAQWLGDILLGNFEALRQVQYSRYTAFNMPDPFVPTPHLVLGLLDAWRTPVKVTAELLMGVRLPPKLKPNEVANIQPSSKRARIEDLPPPTKKLTPELTPLVLFTGFEPVQVQQYIKKLYILGGEVAECTKKCTHLIASKVTRTVKFLTAISVVKHIVTPDWLEECFKRQTFIDEQNYILRDAEAEVLFSFSLEESLKRAHVSPLFKTKYFYITPGICPSLATMKAIVECAGGKVLAKQPSFRKLMEHKQNKSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLDYESYKFN p32114-2 Paired box protein Pax-2 Pax2 Pax-2 protein protein mouse Mus musculus Mouse MDMHCKADPFSAMHRHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQNPTMFAWEIRDRLLAEGICDNDTVPSVSSINRIIRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTASPPVSSASNDPVGSYSINGILGIPRSNGEKRKREEDVSEGSVPNGDSQSGVDSLRKHLRADTFTQQQLEALDRVFERPSYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKSSLSASANPELGSNVSGTQTYPVVTGRDMTSTTLPGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNPYSHPQYTAYNEAWRFSNPALLSSPYYYSAAPRGSAPAAAAYDRH pax8_mouse Paired box protein Pax-8 Pax8 Pax-8 protein protein mouse Mus musculus Mouse MPHNSIRSGHGGLNQLGGAFVNGRPLPEVVRQRIVDLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIRTKVQQPFNLPMDSCVATKSLSPGHTLIPSSAVTPPESPQSDSLGSTYSINGLLGIAQPGNDNKRKMDDSDQDSCRLSIDSQSSSSGPRKHLRTDTFSQHHLEALECPFERQHYPEAYASPSHTKGEQGLYPLPLLNSALDDGKATLTSSNTPLGRNLSTHQTYPVVADPHSPFAIKQETPELSSSSSTPSSLSSSAFLDLQQVGSGGPAGASVPPFNAFPHAASVYGQFTGQALLSGREMVGPTLPGYPPHIPTSGQGSYASSAIAGMVAGSEYSGNAYSHTPYSSYSEAWRFPNSSLLSSPYYYSSTSRPSAPPTSATAFDHL pax6_mouse Paired box protein Pax-6 Pax-6 Sey Pax6 Oculorhombin protein protein mouse Mus musculus Mouse MQNSHSGVNQLGGVFVNGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATPEVVSKIAQYKRECPSIFAWEIRDRLLSEGVCTNDNIPSVSSINRVLRNLASEKQQMGADGMYDKLRMLNGQTGSWGTRPGWYPGTSVPGQPTQDGCQQQEGGGENTNSISSNGEDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTHYPDVFARERLAAKIDLPEARIQVWFSNRRAKWRREEKLRNQRRQASNTPSHIPISSSFSTSVYQPIPQPTTPVSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYWPRLQ pax2_mouse Paired box protein Pax-2 Pax2 Pax-2 protein protein mouse Mus musculus Mouse MDMHCKADPFSAMHRHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQNPTMFAWEIRDRLLAEGICDNDTVPSVSSINRIIRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTASPPVSSASNDPVGSYSINGILGIPRSNGEKRKREEVEVYTDPAHIRGGGGLHLVWTLRDVSEGSVPNGDSQSGVDSLRKHLRADTFTQQQLEALDRVFERPSYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKSSLSASANPELGSNVSGTQTYPVVTGRDMTSTTLPGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNPYSHPQYTAYNEAWRFSNPALLSSPYYYSAAPRGSAPAAAAYDRH pax2-paxip1-2 666454 N/A 666459 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 666460 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false 1 pax2-paxip1-3 666454 N/A 666460 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 666461 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false 1 pax8-paxip1-1 666454 N/A 666460 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 666462 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false 1 pax6-paxip1-1 666454 N/A 666460 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 666463 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 paxip1-pax2-1 666455 N/A 666464 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells N/A 666460 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells physical association physical association false false false 5B pax2-paxip1-4 666455 N/A 666460 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells N/A 666464 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells physical association physical association false false false 5B pax2-paxip1-1 666456 N/A 666459 unspecified role unspecified role bait bait region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 666460 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false no figure for library screen pax2-paxip1-5 666457 N/A 666460 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 666461 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 5C pax2-paxip1-6 666458 N/A 666464 unspecified role unspecified role neutral component neutral component mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells N/A 666460 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 7D and E