IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ corona-2000-2 Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). drome Drosophila melanogaster (Fruit fly) Fruit fly coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Corona DF., Eberharter A., Budde A., Deuring R., Ferrari S., Varga-Weisz P., Wilm M., Tamkun J., Becker PB. Chromatin - Epigenetic interactions resulting in chromatin modulation drosophila embryo extract used in the experiment. EMBO J. (0261-4189) 2000 corona-2000-3 Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). drome Drosophila melanogaster (Fruit fly) Fruit fly coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Corona DF., Eberharter A., Budde A., Deuring R., Ferrari S., Varga-Weisz P., Wilm M., Tamkun J., Becker PB. Chromatin - Epigenetic interactions resulting in chromatin modulation drosophila embryo extract used in the experiment. EMBO J. (0261-4189) 2000 corona-2000-1 Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Corona DF., Eberharter A., Budde A., Deuring R., Ferrari S., Varga-Weisz P., Wilm M., Tamkun J., Becker PB. Chromatin - Epigenetic interactions resulting in chromatin modulation drosophila embryo extract used in the experiment. EMBO J. (0261-4189) 2000 corona-2000-4 Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Corona DF., Eberharter A., Budde A., Deuring R., Ferrari S., Varga-Weisz P., Wilm M., Tamkun J., Becker PB. Chromatin - Epigenetic interactions resulting in chromatin modulation drosophila embryo extract used in the experiment. EMBO J. (0261-4189) 2000 corona-2000-5 Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Corona DF., Eberharter A., Budde A., Deuring R., Ferrari S., Varga-Weisz P., Wilm M., Tamkun J., Becker PB. Chromatin - Epigenetic interactions resulting in chromatin modulation drosophila embryo extract used in the experiment. EMBO J. (0261-4189) 2000 corona-2000-6 Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). ecoli Escherichia coli (strain K12) pull down pull down western blot western blot Immuno blot Immuno blot Corona DF., Eberharter A., Budde A., Deuring R., Ferrari S., Varga-Weisz P., Wilm M., Tamkun J., Becker PB. Chromatin - Epigenetic interactions resulting in chromatin modulation drosophila embryo extract used in the experiment. EMBO J. (0261-4189) 2000 iswi_drome Chromatin-remodeling complex ATPase chain Iswi Iswi CG8625 Protein imitation swi Nucleosome-remodeling factor 140 kDa subunit CHRAC 140 kDa subunit protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSKTDTAAVEATEENSNETTSDAATSSSGEKEAEFDNKIEADRSRRFDFLLKQTEIFTHFMTNSAKSPTKPKGRPKKIKDKDKEKDVADHRHRKTEQEEDEELLAEDSATKEIFRFDASPAYIKSGEMRDYQIRGLNWMISLYENGINGILADEMGLGKTLQTISLLGYLKHFKNQAGPHIVIVPKSTLQNWVNEFKKWCPSLRAVCLIGDQDTRNTFIRDVLMPGEWDVCVTSYEMCIREKSVFKKFNWRYLVIDEAHRIKNEKSKLSEILREFKTANRLLITGTPLQNNLHELWALLNFLLPDVFNSSEDFDEWFNTNTCLGDDALITRLHAVLKPFLLRRLKAEVEKRLKPKKEMKIFVGLSKMQRDWYTKVLLKDIDVVNGAGKVEKMRLQNILMQLRKCTNHPYLFDGAEPGPPYTTDTHLVYNSGKMAILDKLLPKLQEQGSRVLIFSQMTRMLDILEDYCHWRNYNYCRLDGQTPHEDRNRQIQEFNMDNSAKFLFMLSTRAGGLGINLATADVVIIYDSDWNPQMDLQAMDRAHRIGQKKQVRVFRLITESTVEEKIVERAEVKLRLDKMVIQGGRLVDNRSNQLNKDEMLNIIRFGANQVFSSKETDITDEDIDVILERGEAKTAEQKAALDSLGESSLRTFTMDTNGEAGTSSVYQFEGEDWREKQKLNALGNWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPIVQDFQFFPPRLFELLDQEIYYFRKTVGYKVPKNTELGSDATKVQREEQRKIDEAEPLTEEEIQEKENLLSQGFTAWTKRDFNQFIKANEKYGRDDIDNIAKDVEGKTPEEVIEYNAVFWERCTELQDIERIMGQIERGEGKIQRRLSIKKALDQKMSRYRAPFHQLRLQYGNNKGKNYTEIEDRFLVCMLHKLGFDKENVYEELRAAIRASPQFRFDWFIKSRTALELQRRCNTLITLIERENIELEEKERAEKKKKAPKGSVSAGSGSASSNTPAPAPQPKASQKRKSEVVATSSNSKKKKK chrc_drome Chromatin accessibility complex 16kD protein Chrac-16 CHRAC CHRAC16 joey CG15736 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGEPRSQPPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDDDDDDDDDDEEESESESESDE dpoe3_drome DNA polymerase epsilon subunit 3 Chrac-14 CHRAC CHRAC14 mary CG13399 Chromatin accessibility complex 14kD protein DNApolE3 Histone-fold protein CHRAC subunit protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVERIEDLNLPNAVIGRLIKEALPESASVSKEARAAIARAASVFAIFVTSSSTALAHKQNHKTITAKDILQTLTELDFESFVPSLTQDLEVYRKVVKEKKESKASKKDSNTAENANASATATAEEAPE chrac_14-iswi-1 Interaction determined by CoIP. 131979 N/A 131985 unspecified role unspecified role bait bait N/A 131986 unspecified role unspecified role prey prey N/A 131987 unspecified role unspecified role prey prey association association false false false chrac_14-iswi-2 Interaction determined by CoIP. 131979 N/A 131987 unspecified role unspecified role bait bait N/A 131985 unspecified role unspecified role prey prey N/A 131986 unspecified role unspecified role prey prey association association false false false chrac_14-iswi-3 Interaction determined by CoIP in transgenic fly embryo. 131980 N/A 131987 unspecified role unspecified role prey prey N/A 131985 unspecified role unspecified role bait bait N/A 131986 unspecified role unspecified role prey prey association association false false false ISWI HA tagged and expressed in fly as a transgenic gene. chrac_14-iswi-6 Interaction determined by CoIP in transgenic fly embryo. 131980 N/A 131986 unspecified role unspecified role bait bait N/A 131987 unspecified role unspecified role bait bait N/A 131985 unspecified role unspecified role prey prey association association false false false Combined sera raised against CHRAC-14 and CHRAC16 used for the CoIP. chrac_14-chrac_16-2 interaction detected by gel filteration. 131981 N/A 131987 unspecified role unspecified role ancillary ancillary N/A 131985 unspecified role unspecified role neutral component neutral component N/A 131986 unspecified role unspecified role neutral component neutral component physical association physical association false false false chrac_14-chrac_16-3 interaction detected by Y2H. 131982 N/A 131986 unspecified role unspecified role prey prey N/A 131987 unspecified role unspecified role bait bait physical association physical association false false false chrac_14-iswi-5 Interaction determined by GST pull down. 131983 N/A 131986 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) N/A 131987 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 131985 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) association association false false false CHRAC_14 was GST tagged and CHRAC_16 coexpressed and copurified. chrac_14-iswi-4 Interaction determined by GST pull down. 131984 N/A 131987 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 131986 unspecified role unspecified role prey prey physical association physical association false false false CHRAC_14 was GST tagged and coexpressed with CHRAC_16.