IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cairns-1999-2 Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Cairns BR., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg RD., Winston F. Chromatin - Epigenetic interactions resulting in chromatin modulation brad.caims@hci.utah.edu Mol. Cell (1097-2765) 1999 cairns-1999-1 Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast biochemical biochemical edman degradation edman degradation Cairns BR., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg RD., Winston F. Chromatin - Epigenetic interactions resulting in chromatin modulation brad.caims@hci.utah.edu Mol. Cell (1097-2765) 1999 rsc1_yeast Chromatin structure-remodeling complex subunit RSC1 RSC1 RSC complex subunit RSC1 YGR056W Remodel the structure of chromatin complex subunit 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVEQDNGFLQKLLKTQYDAVFHLKDENGIEIYPIFNVLPPKKEYPDYYIIIRNPISLNTLKKRLPHYTSPQDFVNDFAQIPWNAMTYNAKDSVIYKYAILLESFIKGKIVHNIRKHYPEVTYPSLGRIPEIFAESMQPSDLSSNPINTQENDEKAGLNPEMKMAFAKLDSSITERKPTNQDYRMQQKNSPAFPTHSASITPQPLASPTPVVNYANITSAHPKTHVRRGRPPVIDLPYVLRIKNILKMMRREVDQNNKTLTLCFEKLPDRNEEPTYYSVITDPICLMDIRKKVKSRKYRNFHTFEEDFQLMLTNFKLYYSQDQSNIIRAQLLEKNFNRLVRIELSKPDEDYLPEGELRYPLDDVEINDEKYQIGDWVLLHNPNDINKPIVGQIFRLWSTTDGNKWLNACWYFRPEQTVHRVDRLFYKNEVMKTGQYRDHPIQDIKGKCYVIHFTRFQRGDPSTKVNGPQFVCEFRYNESDKVFNKIRTWKACLPEELRDQDEPTIPVNGRKFFKYPSPIADLLPANATLNDKVPEPTEGAPTAPPLVGAVYLGPKLERDDLGEYSTSDDCPRYIIRPNDPPEEGKIDYETGTIITDTLTTSSMPRVNSSSTIRLPTLKQTKSIPSSNFRSSSNTPLLHQNFNQTSNFLKLENMNNSSHNLLSHPSVPKFQSPSLLEQSSRSKYHSAKKQTQLSSTAPKKPASKSFTLSSMINTLTAHTSKYNFNHIVIEAPGAFVVPVPMEKNIRTIQSTERFSRSNLKNAQNLGNTAINDINTANEQIIWFKGPGVKITERVIDSGNDLVRVPLNRWFCKNKRRKLDYEDIEEDVMEPPNDFSEDMIANIFNPPPSLNLDMDLNLSPSSNNSSNFMDLSTIASGDNDGKECDTAEESEDENEDTEDEHEIEDIPTTSAFGLNSSAEYLAFRLREFNKL sth1_yeast Nuclear protein STH1/NPS1 STH1 NPS1 ATP-dependent helicase STH1 Chromatin structure-remodeling complex protein STH1 SNF2 homolog YIL126W protein protein yeast Saccharomyces cerevisiae Baker's yeast MLQEQSELMSTVMNNTPTTVAALAAVAAASETNGKLGSEEQPEITIPKPRSSAQLEQLLYRYRAIQNHPKENKLEIKAIEDTFRNISRDQDIYETKLDTLRKSIDKGFQYDEDLLNKHLVALQLLEKDTDVPDYFLDLPDTKNDNTTAIEVDYSEKKPIKISADFNAKAKSLGLESKFSNATKTALGDPDTEIRISARISNRINELERLPANLGTYSLDDCLEFITKDDLSSRMDTFKIKALVELKSLKLLTKQKSIRQKLINNVASQAHHNIPYLRDSPFTAAAQRSVQIRSKVIVPQTVRLAEELERQQLLEKRKKERNLHLQKINSIIDFIKERQSEQWSRQERCFQFGRLGASLHNQMEKDEQKRIERTAKQRLAALKSNDEEAYLKLLDQTKDTRITQLLRQTNSFLDSLSEAVRAQQNEAKILHGEEVQPITDEEREKTDYYEVAHRIKEKIDKQPSILVGGTLKEYQLRGLEWMVSLYNNHLNGILADEMGLGKTIQSISLITYLYEVKKDIGPFLVIVPLSTITNWTLEFEKWAPSLNTIIYKGTPNQRHSLQHQIRVGNFDVLLTTYEYIIKDKSLLSKHDWAHMIIDEGHRMKNAQSKLSFTISHYYRTRNRLILTGTPLQNNLPELWALLNFVLPKIFNSAKTFEDWFNTPFANTGTQEKLELTEEETLLIIRRLHKVLRPFLLRRLKKEVEKDLPDKVEKVIKCKLSGLQQQLYQQMLKHNALFVGAGTEGATKGGIKGLNNKIMQLRKICNHPFVFDEVEGVVNPSRGNSDLLFRVAGKFELLDRVLPKFKASGHRVLMFFQMTQVMDIMEDFLRMKDLKYMRLDGSTKTEERTEMLNAFNAPDSDYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITTDSVEEVILERAMQKLDIDGKVIQAGKFDNKSTAEEQEAFLRRLIESETNRDDDDKAELDDDELNDTLARSADEKILFDKIDKERMNQERADAKAQGLRVPPPRLIQLDELPKVFREDIEEHFKKEDSEPLGRIRQKKRVYYDDGLTEEQFLEAVEDDNMSLEDAIKKRREARERRRLRQNGTKENEIETLENTPEASETSLIENNSFTAAVDEETNADKETTASRSKRRSSRKKRTISIVTAEDKENTQEESTSQENGGAKVEEEVKSSSVEIINGSESKKKKPKLTVKIKLNKTTVLENNDGKRAEEKPESKSPAKKTAAKKTKTKSKSLGIFPTVEKLVEEMREQLDEVDSHPRTSIFEKLPSKRDYPDYFKVIEKPMAIDIILKNCKNGTYKTLEEVRQALQTMFENARFYNEEGSWVYVDADKLNEFTDEWFKEHSS 269A91BD853C20D0 rsc2_yeast Chromatin structure-remodeling complex subunit RSC2 RSC2 RSC complex subunit RSC2 L9638.1 YLR357W Remodel the structure of chromatin complex subunit 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MMPDDNSNSSTQNSSALYKDLRKEYESLFTLKEDSGLEISPIFNVLPPKKDYPDYYAVIKNPVSFNTLKKRIPHYTDAQQFMNDVVQIPWNAKTYNTRDSGIYKYALVLEKYLKDTIYPNLKEKYPQLVYPDLGPLPDEPGYEEFQQKLREKAEEVARANAARAESSSSMNSTEAARRLRKTRTSVKRESEPGTDTNNDEDYEATDMDIDNPKDADFPDLIRKPLININPYTRKPLRDNRSTTPSHSGTPQPLGPRHRQVSRTQVKRGRPPIIDLPYIQRMKNVMKVLKKEVLDSGIGLTDLFERLPDRHRDANYYIMIANPISLQDINKKVKTRRYKTFQEFQNDFNLMLTNFRISHRGDPESIKISNILEKTFTSLARFELSKPDRSFIPEGELRYPLDEVIVNNISYHVGDWALLRNQNDPQKPIVGQIFRLWKTPDGKQWLNACWYYRPEQTVHRVDRLFYKNEVMKTGQYRDHLVSNLVGKCYVIHFTRYQRGNPDMKLEGPLFVCEFRYNESDKIFNKIRTWKACLPEEIRDLDEATIPVNGRKFFKYPSPIRHLLPANATPHDRVPEPTMGSPDAPPLVGAVYMRPKMQRDDLGEYATSDDCPRYIIRPNDSPEEGQVDIETGTITTNTPTANALPKTGYSSSKLSSLRYNRSSMSLENQNAIGQQQIPLSRVGSPGAGGPLTVQGLKQHQLQRLQQQQHQYQQQKRSQASRYNIPTIIDDLTSQASRGNLGNIMIDAASSFVLPISITKNVDVLQRTDLHSQTKRSGREEMFPWKKTKGEILWFRGPSVIVNERIINSGDPHLSLPLNRWFTTNKKRKLEYEEVEETMEDVTGKDKDDDGLEPDVENEKESLPGPFVLGLRPSAKFTAHRLSMLRPPSSSS 7867866FD973F188 rsc1-sth1-1 co-immunoprecipitation of rsc complex proteins. bait= yeast rsc1, prey= yeast sth1 14018 N/A 14020 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 14021 unspecified role unspecified role prey prey physical association physical association false false false Deletion of the C-terminal amino acids L496-L911 abolishes this interaction. rsc2-sth1-1 co-immunoprecipitation of rsc complex proteins. bait= yeast rsc2, prey= yeast sth1 14018 N/A 14022 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 14021 unspecified role unspecified role prey prey physical association physical association false false false Deletion of the C-terminal amino acids P580-S889 abolishes this interaction rsc1-rsc2-1 rsc1-rsc2-1 14019 N/A 14020 unspecified role unspecified role neutral component neutral component N/A 14022 unspecified role unspecified role neutral component neutral component physical association physical association false false false