IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ eberharter-1999-1 The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column ms participant Identification by mass spectrometry ms participant Eberharter A., Sterner DE., Schieltz D., Hassan A., Yates JR., Berger SL., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Yeast CY396 strain expressing HA and His tagged SWI2 was used for purification of the complex. The complex was purified using the following sequential chromatographic steps: Ni NTA-agarose, MonoQ, Mono S, Superose 6, DNA cellulose, Histone agaorose, Superose 6, Mini Q and Superose 6 PC 3.2/30. Mol. Cell. Biol. (0270-7306) 1999 eberharter-1999-2 The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Eberharter A., Sterner DE., Schieltz D., Hassan A., Yates JR., Berger SL., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Yeast CY396 strain expressing HA and His tagged SWI2 was used for purification of the complex. The complex was purified using the following sequential chromatographic steps: Ni NTA-agarose, MonoQ, Mono S, Superose 6, DNA cellulose, Histone agaorose, Superose 6, Mini Q and Superose 6 PC 3.2/30. Mol. Cell. Biol. (0270-7306) 1999 eberharter-1999-3 The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom western blot western blot Immuno blot Immuno blot Eberharter A., Sterner DE., Schieltz D., Hassan A., Yates JR., Berger SL., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Yeast CY396 strain expressing HA and His tagged SWI2 was used for purification of the complex. The complex was purified using the following sequential chromatographic steps: Ni NTA-agarose, MonoQ, Mono S, Superose 6, DNA cellulose, Histone agaorose, Superose 6, Mini Q and Superose 6 PC 3.2/30. Mol. Cell. Biol. (0270-7306) 1999 eberharter-1999-4 The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom western blot western blot Immuno blot Immuno blot Eberharter A., Sterner DE., Schieltz D., Hassan A., Yates JR., Berger SL., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Yeast CY396 strain expressing HA and His tagged SWI2 was used for purification of the complex. The complex was purified using the following sequential chromatographic steps: Ni NTA-agarose, MonoQ, Mono S, Superose 6, DNA cellulose, Histone agaorose, Superose 6, Mini Q and Superose 6 PC 3.2/30. Mol. Cell. Biol. (0270-7306) 1999 ngg1_yeast Chromatin-remodeling complexes subunit NGG1 NGG1 ADA3 YDR176W YD9395.09 SWI7 Transcriptional adapter 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPRHGRRGKLPKGEKLPKKEGGDNTPSKLLSSMLKTLDLTFERDIGMLNGKSVRSIPNKKTLLELQSQLDSLNEILGTIARGDQETIEALRKIRDSKNEKQANDEKQETSNADGQHESSTATEETNIIDKGVQSPPKPPPSNEISGTIENDVESIKQAADNMAKEEINEDKDLQVHRDQPREKRPFDSETENRATENENTQRPDNKKQKIDVDKMENDPTVKNPKSEFVVSQTLPRAAAALGLFNEEGLESTGEDFLKKKYNVASYPTNDLKDLLPGELPDMDFSHPKPTNQIQFNTFLAFVENFFKDLSDDNLKFLKMKYIIPDSLQFDKTYDPEVNPFIIPKLGPLYTDVWFKDENDKNSAYKKPSPYSNDASTILPKKSANELDDNALESGSISCGPLLSRLLSAVLKDDNDKSELQSSKIIRDGGLPRTGGEDDIQSFRNNNNDTVDMTLSQENGPSVQTPDNDIDEEASFQAKLAENKGSNGGTTSTLPQQIGWITNGINLDYPTFEERLKRELKYVGIYMNLPKDENNPNSDDPDWVTGREDDEISAELRELQGTLKQVTKKNQKRKAQLIPLVERQLAWQEYSSILEDLDKQIDQAYVKRIRVPKKRKKHHTAASNNVNTGTTSQIAQQKAANSSLKSLLDKRQRWINKIGPLFDKPEIMKRIPNESVFKDMDQEEDEDEADVFAQNTNKDVELN 489016BFD1C095D4 ahc1_yeast Protein AHC1 AHC1 OR26.13 ADA HAT complex component 1 YOR023C protein protein yeast Saccharomyces cerevisiae Baker's yeast MMSPAQDKLQHQHHNPNSSSSSSSKMTNVYQVTTPKSPQDLENNMDEPFKMDTATSNPDKDSENTQRLKYECAKGEIQNVLNLHIMLNHKHVRHLRRNVQKVNAKLALLETLHKDTGLLNKIERTYQLKIKQHQQHSVLGGHFHDSTATENTNASNYNLSYPVLSDYNINCQPLSSSSNRNLSTTRIPHHHYHTRSKSNGLLLEPSALRPANSNIIDYRLTGSKSLSEAITKPTPVSLPHSNSDGISSPRSSSISPLDEQPGFQILPFKPSQMHLNHRRNYSSTCLTSNSGIIGKTENNEPIFRRYDGILVIITCSKCDRSGFTSAQGIVNHTRLKHSKLYSSQPLAVLNNQKLLPNDKQDPEILSKFKKLNLDPNKDYLPSDIAIPKPQSPINHSENHTRAPKTVKNTPHLEKLYQNKEDFKKLIDMVNETPDDLNEYLKQREIQLRYQKEQEEESSKSDDEASYVPSPSLSATATTTTTTDPPSPPVLSSSLQRKLLRKRKLSLNSSTPMEDLPLRERLRANPTDKKPRKAALLTNELEGPDPAAKSSSYYNLRSKSRLRGSHT C18AFA10E4334764 gcn5_yeast Histone acetyltransferase GCN5 YGR252W GCN5 ADA4 SWI9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTKHQIEEDHLDGATTDPEVKRVKLENNVEEIQPEQAETNKQEGTDKENKGKFEKETERIGGSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEFRVVNNDNTKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGGITYRPFDKREFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAIGYFKKQGFTKEITLDKSIWMGYIKDYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRRKIRTISKSHIVRPGLEQFKDLNNIKPIDPMTIPGLKEAGWTPEMDALAQRPKRGPHDAAIQNILTELQNHAAAWPFLQPVNKEEVPDYYDFIKEPMDLSTMEIKLESNKYQKMEDFIYDARLVFNNCRMYNGENTSYYKYANRLEKFFNNKVKEIPEYSHLID 3200730DDC7EF70D ada2_yeast Transcriptional adapter 2 ADA2 D9461.33 YDR448W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNKFHCDVCSADCTNRVRVSCAICPEYDLCVPCFSQGSYTGKHRPYHDYRIIETNSYPILCPDWGADEELQLIKGAQTLGLGNWQDIADHIGSRGKEEVKEHYLKYYLESKYYPIPDITQNIHVPQDEFLEQRRHRIESFRERPLEPPRKPMASVPSCHEVQGFMPGRLEFETEFENEAEGPVKDMVFEPDDQPLDIELKFAILDIYNSRLTTRAEKKRLLFENHLMDYRKLQAIDKKRSKEAKELYNRIKPFARVMTAQDFEEFSKDILEELHCRARIQQLQEWRSNGLTTLEAGLKYERDKQARISSFEKFGASTAASLSEGNSRYRSNSAHRSNAEYSQNYSENGGRKKNMTISDIQHAPDYALLSNDEQQLCIQLKILPKPYLVLKEVMFRELLKTGGNLSKSACRELLNIDPIKANRIYDFFQSQNWM 9637E1EDBBED0AC3 ada2-ada3-2 588060 N/A 588064 unspecified role unspecified role neutral component neutral component N/A 588065 unspecified role unspecified role neutral component neutral component N/A 588066 unspecified role unspecified role neutral component neutral component N/A 588067 unspecified role unspecified role neutral component neutral component physical association physical association false false false 2 ada2-ada3-3 588061 N/A 588065 unspecified role unspecified role neutral component neutral component N/A 588067 unspecified role unspecified role neutral component neutral component N/A 588064 unspecified role unspecified role neutral component neutral component physical association physical association false false false 3 ada2-ada3-4 588062 N/A 588065 unspecified role unspecified role neutral component neutral component N/A 588064 unspecified role unspecified role neutral component neutral component N/A 588066 unspecified role unspecified role neutral component neutral component N/A 588067 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4B ada2-gcn5 588063 N/A 588067 unspecified role unspecified role neutral component neutral component N/A 588066 unspecified role unspecified role neutral component neutral component physical association physical association false false false 5