IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ taranum-2011-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Taranum S., Sur I., Müller R., Lu W., Rashmi RN., Munck M., Neumann S., Karakesisoglou I., Noegel AA. Cardiac - Interactions involving cardiac related proteins Figure 4 F-Actin Binding co-sedimentation assay results have not been entered as the identity of F-Actin cannot be determined. noegel@uni-koeln.de International journal of cell biology 2011 Only protein-protein interactions imex curation imex curation taranum-2011-2 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Taranum S., Sur I., Müller R., Lu W., Rashmi RN., Munck M., Neumann S., Karakesisoglou I., Noegel AA. Cardiac - Interactions involving cardiac related proteins Figure 4 F-Actin Binding co-sedimentation assay results have not been entered as the identity of F-Actin cannot be determined. noegel@uni-koeln.de International journal of cell biology 2011 Only protein-protein interactions imex curation imex curation q6zwr6-5 Nesprin-1 Enaptin Myocyte nuclear envelope protein 1 Nuclear envelope spectrin repeat protein 1 Synaptic nuclear envelope protein 1 Enaptin-165 Syne1 KASH domain-containing protein 1 protein protein mouse Mus musculus Mouse MATSRASSRSHRDITNVMQRLQDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGIKLLALLEVLSGQKLPCEQGHRVKRIHAVANIGTALKFLEGRKSMYRGSPIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSMVSTETASPPSKRKVAAKIQGNAKKTLLKWVQHTAGKQMGIEVKDFGKSWRTGLAFHSVIHAIQPELVDLEKVKTRSNRENLEDAFTIAETQLGIPRLLDPEDVDVDKPDEKSIMTYVAQFLTQYPDIHGAGCDGQEDDRDDRLILKETKVWIEQFERDFTRAQMTESSLQDKYQAFKHFRVQYEMKRKQVEHIIQPLQRDGKLTLDQALVKQCWERVSSRLFDWHIQLDKSLPAPLGTIGAWLYRAEVALREEITIQQVHEETANTIQRKLEQHKDLLQNTDAHKRAFHEIYQTRSVNGIPMPPDQLEDMAERFHFVSSTSELHLMKMEFLELKYRLLSLLVLAESKLKSWIIKYGRRESVELLLQSYISFIENSKFFEQYEVTYQILKQTADIYVKAEGSVEEAENVMKFMSEATAQWRNLSVEVRSVRSMLEEVISNWDRYGDTVASLQAWLEDAEKMLSQSEHAKKDFFRNLPHWIQQHTAMNDAGNFLIETCDEIVSRDLKQQLLLLNGRWRELFMEVKQYARADEMDRMKKEYIDVTTTLFGFATEAHRKLSEPLEVSFINVKLLIQDLEDLEKRVPVMDAQYKMIAKKAHLFAKESPQEEANEMLTTMSKLKEQLSKVKECCSPLLYEAQQLTVPLEELETQITSFYDSLGKINEILSVLEQEAQSSTLFKQKHQELLASQENCKKSLTLIEKGSQSVQKLVTSSQARKPWDHTKLQKQIADVHHAFQSMIKKTGDWKKHVEANSRLMKKFEESRAELEKVLRVAQEGLEEKGDPEELLRRHTEFFSQLDQRVLNAFLKACDELTDILPEQEQQGLQEAVRKLHKQWKDLQGEAPYHLLHLKIAVEKDRFSAAVEECRAELEQETKLAPQEGSEKIIKEHRVFFSDKGPHHLCEKRLQLIEELCGKLPVQDPVRDTCGACHTALKELKASIDNTYTMLVDDPDKWKDYTSRFSEFSSWVSAKKACLKKIKDEPIDTGNHDEVKHMVDEIRNDITKKGESLSWLKSRLKYLIDISSENEAQKRGDELAELSSSFKALVALLSEVEKLLSNFGECVQYKEIVKSSLEGLISGPQESKEEAEMILDSKNLLEAQQLLLHHQQKTKMISAKKRDLQEQMEQAQQGGQAGPGQEELRKLESTLTGLEQSRERQERRIQVSLRKWERFETNKETVVRYLFQTGSSHERFLSFSSLESLSSELEQTKEFSKRTESIATQAENLVKEAAELPLGPRNKRVLQRQAKSIKEQVTTLEDTLEEEYVLHHF q6zmz3-1 Nesprin-3 Nuclear envelope spectrin repeat protein 3 Alpha SYNE3 C14orf49 C14orf139 LINC00341 KASH domain-containing protein 3 protein protein human Homo sapiens Human MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITGELEEMRKVLEKLRALWEEEEERLRGLLRSRGAWEQQIKQLEAELSEFRMVLQRLAQEGLQPAAKAGTEDELVAHWRRYSATRAALASEEPRVDRLQAQLKELIVFPHNLKPLSDSVIATIQEYQSLKVKSARLRNAAAVELWQHFQRPLQDLQLWKALAQRLLEVTASLPDLPSLHTFLPQIEAALMESSRLKELLTMLQLKKDLLIGIFGQERATALLEQVAGSMRDRDLLHNSLLQRKSKLQSLLAQHKDFGAAFEPLQRKLLDLQVRVQAEKGLQRDLPGKQAQLSRLQGLQEEGLDLGAQMEAARPLVQENPNHQHKMDQLSSDFQALQRSLEDLVDRCRQSVQEHCTFSHQLLELRQWIVVTTQKLEAHRGEAGPGDAESQEAEFERLVAEFPEKEAQLSLVEAQGWLVMEKSSPEGAAVVQEELRELAESWRALRLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWRGLGSLFRRACCVALPLQLLLLLFLLLLFLLPIREEDRSCTLANNFARSFTLMLRYNGPPPT syne1-syne1-6 1791515 N/A 1791517 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false actin_binding_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions3/4/5/6 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions6/7/8/9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions9/10/11 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 1791517 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false actin_binding_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions3/4/5/6 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions6/7/8/9 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions9/10/11 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1791528 1791522 1791529 1791523 1791530 1791524 1791531 1791525 physical association physical association false false false 2B syne1-syne1-7 1791515 N/A 1791517 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 1791517 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false actin_binding_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions3/4/5/6 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions9/10/11 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 2C syne1-syne1-8 1791515 N/A 1791518 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 N/A 1791517 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false actin_binding_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions3/4/5/6 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions9/10/11 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 3A syne1-syne3-1 1791516 N/A 1791518 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1791517 unspecified role unspecified role bait bait actin_binding_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions3/4/5/6 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions9/10/11 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false sr_regions6/7/8/9 [859-1144] sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B The authors considered the reaction with bait molecule to be a false positive. q6zmz3-1 region 859-1144 to