bhf-ucl ashpole-2012-1 Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates cardiac sodium channel NaV1.5 gating by multiple phosphorylation sites. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ashpole N.M., Herren A.W., Ginsburg K.S., Brogan J.D., Johnson D.E., Cummins T.R., Bers D.M., Hudmon A. Cardiac - Interactions involving cardiac related proteins Methods state: GST-tagged intracellular regions of NaV1.5 (N terminus, L1–L3, and C terminus). Therefore assumed L1 (loop1) is 416 – 711aa based on uniprot record cytoplasmic domain, and this has 594T within L1. ahudmon@iupui.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation ashpole-2012-6 Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates cardiac sodium channel NaV1.5 gating by multiple phosphorylation sites. in vitro In vitro protein kinase assay protein kinase assay tag visualisation tag visualisation Ashpole N.M., Herren A.W., Ginsburg K.S., Brogan J.D., Johnson D.E., Cummins T.R., Bers D.M., Hudmon A. Cardiac - Interactions involving cardiac related proteins Methods state: GST-tagged intracellular regions of NaV1.5 (N terminus, L1–L3, and C terminus). Therefore assumed L1 (loop1) is 416 – 711aa based on uniprot record cytoplasmic domain, and this has 594T within L1. ahudmon@iupui.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation ashpole-2012-3 Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates cardiac sodium channel NaV1.5 gating by multiple phosphorylation sites. in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Ashpole N.M., Herren A.W., Ginsburg K.S., Brogan J.D., Johnson D.E., Cummins T.R., Bers D.M., Hudmon A. Cardiac - Interactions involving cardiac related proteins Methods state: GST-tagged intracellular regions of NaV1.5 (N terminus, L1–L3, and C terminus). Therefore assumed L1 (loop1) is 416 – 711aa based on uniprot record cytoplasmic domain, and this has 594T within L1. ahudmon@iupui.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation ashpole-2012-2 Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates cardiac sodium channel NaV1.5 gating by multiple phosphorylation sites. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down monoclonal western monoclonal antibody western blot monoclonal western Ashpole N.M., Herren A.W., Ginsburg K.S., Brogan J.D., Johnson D.E., Cummins T.R., Bers D.M., Hudmon A. Cardiac - Interactions involving cardiac related proteins Methods state: GST-tagged intracellular regions of NaV1.5 (N terminus, L1–L3, and C terminus). Therefore assumed L1 (loop1) is 416 – 711aa based on uniprot record cytoplasmic domain, and this has 594T within L1. ahudmon@iupui.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation ashpole-2012-4 Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates cardiac sodium channel NaV1.5 gating by multiple phosphorylation sites. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip monoclonal western monoclonal antibody western blot monoclonal western Ashpole N.M., Herren A.W., Ginsburg K.S., Brogan J.D., Johnson D.E., Cummins T.R., Bers D.M., Hudmon A. Cardiac - Interactions involving cardiac related proteins Methods state: GST-tagged intracellular regions of NaV1.5 (N terminus, L1–L3, and C terminus). Therefore assumed L1 (loop1) is 416 – 711aa based on uniprot record cytoplasmic domain, and this has 594T within L1. ahudmon@iupui.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation ashpole-2012-5 Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates cardiac sodium channel NaV1.5 gating by multiple phosphorylation sites. in vitro In vitro pull down pull down tag fluorescence tag visualisation by fluorescence tag fluorescence Ashpole N.M., Herren A.W., Ginsburg K.S., Brogan J.D., Johnson D.E., Cummins T.R., Bers D.M., Hudmon A. Cardiac - Interactions involving cardiac related proteins Methods state: GST-tagged intracellular regions of NaV1.5 (N terminus, L1–L3, and C terminus). Therefore assumed L1 (loop1) is 416 – 711aa based on uniprot record cytoplasmic domain, and this has 594T within L1. ahudmon@iupui.edu J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation scn5a_human Sodium channel protein type 5 subunit alpha Sodium channel protein type V subunit alpha Voltage-gated sodium channel subunit alpha Nav1.5 Sodium channel protein cardiac muscle subunit alpha SCN5A hH1 protein protein human Homo sapiens Human MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVNSHERRSKRRKRMSSGTEECGEDRLPKSDSEDGPRAMNHLSLTRGLSRTSMKPRSSRGSIFTFRRRDLGSEADFADDENSTAGESESHHTSLLVPWPLRRTSAQGQPSPGTSAPGHALHGKKNSTVDCNGVVSLLGAGDPEATSPGSHLLRPVMLEHPPDTTTPSEEPGGPQMLTSQAPCVDGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRDSDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDREMNNLQLALARIQRGLRFVKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQQESQPVSGGPEAPPDSRTWSQVSATASSEAEASASQADWRQQWKAEPQAPGCGETPEDSCSEGSTADMTNTAELLEQIPDLGQDVKDPEDCFTEGCVRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSAMVIQRAFRRHLLQRSLKHASFLFRQQAGSGLSEEDAPEREGLIAYVMSENFSRPLGPPSSSSISSTSFPPSYDSVTRATSDNLQVRGSDYSHSEDLADFPPSPDRDRESIV This sequence has been withdrawn from Uniprot. kcc2d_human Calcium/calmodulin-dependent protein kinase type II subunit delta CAMK2D CAMKD protein protein human Homo sapiens Human MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWHRRDGKWQNVHFHRSGSPTVPIKPPCIPNGKENFSGGTSLWQNI kcc2a_rat Calcium/calmodulin-dependent protein kinase type II subunit alpha Camk2a protein protein rat Rattus norvegicus (Rat) Rat MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKNDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH syntide_2_peptide Syntide-2 synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) PLARTLSVAGLPGKK An amino acid sequence which is not represented in UniProt The native peptide PLARTLSVAGLPGKK is a selective Substrate for CaM kinase II and PKC. it is also a poor substrate for phosphorylase kinase and is not phosphorylated by myosin light chain kinase. The sequence of PLARTLSVAGLPGKK is homologous to phosphorylation site 2 in glycogen synthase. camk2g-scn5a 1554962 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role loop l1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false Fig 1C camk2d-scn5a-1 1554962 n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role intein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a NaV1.5 1554968 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified Q14524:p.[Ser516Ala;Thr594Ala] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q14524:p.Thr594Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false loop l1 sufficient to bind sufficient binding region sufficient to bind autoradiography autoradiography certain certain sequence position certain certain certain sequence position certain false Q14524:p.Ser516Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Fig 4A-B Q14524 ser-516 O-phospho-L-serine Q14524 thr-594 O-phospho-L-threonine camk2d-scn5a-5 1554962 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role Q14524:p.Ser516Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q14524:p.Thr594Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false loop l1 sufficient to bind sufficient binding region sufficient to bind autoradiography autoradiography certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q14524:p.Ser516Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q14524:p.Thr594Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role intein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 phosphorylation phosphorylation reaction phosphorylation false false false Fig 4D Q14524 ser-516 O-phospho-L-serine Q14524 thr-594 O-phospho-L-threonine camk2d-scn5a-12 1554962 n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role intein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a NaV1.5 1554968 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified loop l1 sufficient to bind sufficient binding region sufficient to bind autoradiography autoradiography certain certain sequence position certain certain certain sequence position certain false Q14524:p.Thr594Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q14524:p.Ser516Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Fig 4C Q14524 ser-516 O-phospho-L-serine Q14524 thr-594 O-phospho-L-threonine camk2a-scn5a 1554962 n/a 1554968 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a alpha-CaMKII 1554970 enzyme enzyme unspecified role unspecified role spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false Fig 1B camk2d-scn5a-4 1554963 n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role intein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q14524 ser-516 O-phospho-L-serine chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation false false false Fig 3C column 3 Q14524 ser-516 O-phospho-L-serine camk2d-scn5a-4 1554963 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q14524:p.Thr594Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q14524:p.Ser593Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false Q14524:p.Ser593_Thr594delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role intein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 phosphorylation phosphorylation reaction phosphorylation false false false Fig 3F Q14524 thr-594 O-phospho-L-threonine camk2d-scn5a-8 1554963 n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role purified purified autophosphorylation allosteric ptm allosteric post-translational modification undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false autophosphorylation region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a NaV1.5 1554968 monoclonal western monoclonal antibody western blot monoclonal western ancillary ancillary unspecified role unspecified role loop l1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1554971 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified synthetic construct synthetic construct phosphorylation phosphorylation reaction phosphorylation false false false Fig 2D A significant increase in syntide-2 phosphorylation was only observed when autophosphorylated CaMKII was incubated with and allowed to bind SCN5A. camk2d-scn5a-9 1554963 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false loop l1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false Fig 1D camk2d-scn5a-13 1554963 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q14524 ser-593 O-phospho-L-serine Q14524 thr-594 O-phospho-L-threonine chemical synthesis Chemical synthesis (Chemical synthesis) n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role intein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 phosphorylation phosphorylation reaction phosphorylation false false false Fig 3C column 5 Q14524 ser-593 O-phospho-L-serine Q14524 thr-594 O-phospho-L-threonine camk2d-scn5a-4 1554964 n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role required autophosphorylation nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false autophosphorylation region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q14524 ser-483 O-phospho-L-serine Q14524 ser-484 O-phospho-L-serine chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation false false false Fig 3A-B spot 25-31 Q14524 ser-483 O-phospho-L-serine Q14524 ser-484 O-phospho-L-serine camk2d-scn5a-4 1554964 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role Q14524:p.Ser516Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false Q14524:p.Thr594Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false Q14524:p.[Ser516Ala;Thr594Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false loop l1 sufficient to bind sufficient binding region sufficient to bind autoradiography autoradiography certain certain sequence position certain certain certain sequence position certain false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role intein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 phosphorylation phosphorylation reaction phosphorylation false false false Fig 4A-D Q14524 Ser-516 O-phospho-L-serine Q14524 Thr-594 O-phospho-L-threonine camk2d-scn5a-10 1554964 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q14524 ser-516 O-phospho-L-serine chemical synthesis Chemical synthesis (Chemical synthesis) n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role required autophosphorylation nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false autophosphorylation region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false Fig 3A-B spot 45-55 Q14524 ser-516 O-phospho-L-serine camk2d-scn5a-11 1554964 n/a NaV1.5 1554968 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q14524 ser-593 O-phospho-L-serine Q14524 thr-594 O-phospho-L-threonine chemical synthesis Chemical synthesis (Chemical synthesis) n/a deltaC-CaMKII 1554969 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false required autophosphorylation nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false autophosphorylation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation false false false Fig 3A-B spot 80-88 Q14524 ser-593 O-phospho-L-serine Q14524 thr-594 O-phospho-L-threonine scn5a-camk2g 1554965 n/a deltaC-CaMKII 1554969 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false required autophosphorylation nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false autophosphorylation Stoichiometry: 1.0 n/a NaV1.5 1554968 unspecified role unspecified role bait bait loop l1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false Fig 2B camk2g-scn5a-3 1554966 n/a NaV1.5 1554968 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role prey prey n/a deltaC-CaMKII 1554969 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 2A scn5a-camk2d 1554967 n/a deltaC-CaMKII 1554969 unspecified role unspecified role prey prey purified purified required autophosphorylation nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false autophosphorylation region fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection Stoichiometry: 1.0 n/a NaV1.5 1554968 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false loop l1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false Fig 2C