IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gehmlich-2011-3 A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. katja.gehmlich@cardiov.ox.ac.uk Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation gehmlich-2011-2 A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. katja.gehmlich@cardiov.ox.ac.uk Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation gehmlich-2011-1 A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. rat-cardiac_myocytes_neo rat cardiac myocytes neo neonatal_ventricular_myocyte neonatal ventricular myocyte A heart muscle cell. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. katja.gehmlich@cardiov.ox.ac.uk Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation gehmlich-2011-4 A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 pull down pull down anti tag western anti tag western blot anti tag western Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. katja.gehmlich@cardiov.ox.ac.uk Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation gehmlich-2011-6 A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 pull down pull down western blot western blot Immuno blot Immuno blot Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. katja.gehmlich@cardiov.ox.ac.uk Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation gehmlich-2011-7 A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. katja.gehmlich@cardiov.ox.ac.uk Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation gehmlich-2011-10 A novel desmocollin-2 mutation reveals insights into the molecular link between desmosomes and gap junctions. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gehmlich K., Lambiase P.D., Asimaki A., Ciaccio E.J., Ehler E., Syrris P., Saffitz J.E., McKenna W.J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction. katja.gehmlich@cardiov.ox.ac.uk Heart Rhythm (1547-5271) 2011 Only protein-protein interactions imex curation q02487-1 Desmocollin-2 DGII DSC2 CDHF2 DSC3 Cadherin family member 2 Desmocollin-3 Desmosomal glycoprotein II Desmosomal glycoprotein III protein protein human Homo sapiens Human MEAARPSGSWNGALCRLLLLTLAILIFASDACKNVTLHVPSKLDAEKLVGRVNLKECFTAANLIHSSDPDFQILEDGSVYTTNTILLSSEKRSFTILLSNTENQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPIPCSMLENSLGPFPLFLQQVQSDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFEIIAFATTPDGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTMHTRLKYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFGLQTTSTCIINIDDVNDHLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRANYTILKGNENGNFKIVTDAKTNEGVLCVVKPLNYEEKQQMILQIGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTSNGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCTGTLGIILQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAINDTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVDPRIGGGGVQLGKWAILAILLGIALLFCILFTLVCGASGTSKQPKVIPDDLAQQNLIVSNTEAPGDDKVYSANGFTTQTVGASAQGVCGTVGSGIKNGGQETIEMVKGGHQTSESCRGAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEKVYLCNQDENHKHAQDYVLTYNYEGRGSVAGSVGCCSERQEEDGLEFLDNLEPKFRTLAEACMKR jup_chlae_protein Green monkey plakoglobin protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey cxa1_chlae Gap junction alpha-1 protein GJA1 Connexin-43 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHTTSGALSPTKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI f1m7l9_rat protein protein rat Rattus norvegicus (Rat) Rat MAIPGSLGECGYIRTVLGQQILGHLDSSSLALPSEARLRLAGSSGRGDPVARSQRIQEQVQQTLARRGRSSVVGGNLHRTSSVPEYVYKLHLVENDFVGRQSPAARDYDMQKTYLTSSFVSGWRDGSTLNSIKPHREKKTWIEALRELEHTTDHTDCPAHYVYSHYYFNTRSHIVSSGKKKLAVILLSRILKLSRSCTHQLAPLRSSVLPSLFNQLQLSPELYFSNMGTLYSSYQLLKGKLPAKHQANFVTMAERSVAVKFPDTSSETSFRRTGDTLPSAWQSRVHGLHFHHVICDCLSRNADLEMTLERAVNMLDADHVPLSKISAAATFIQHESFQKSEARKRVTKCQCVYSTLQYKQCQNSDEDSRECGELRSLVLRDNDPKVNITKREGGGQAAGYMKETFKIEANKNITGLLWNLSSSDKLKHLMITEALLTLTESVIIPFSGWPEGDYPKANGLLDFDIFYNVTGCLRNMSSAGPDGRKVMRRCDGLIDSLVHYVRGTIADYQPDDKATENCVCILHNLSYQLEAELPEKYSQSIYMQNRNIQTNSNKSIGCFGSRSRKVKEQYQDTPMPEERSSPRGIEWLWHSIVIRMYLSLIAKSSRNYTQEASLGALQNLTAGSGPIPTSVARMVVQKENGLQHTRKMLHVGDPSVKKTAVSLLRNLSRNLSLQNEIAKETLPDLVSIIPDTVPSTDLLIETTASACYTLNNLMQNSYQNARDLLNTGGLQKIMTISIGEGYAPNKASKAASVLLYSLWAHTELHNAYKKAQFKKTDFVNSRTAKASHSLKD plak_rat Junction plakoglobin Jup protein protein rat Rattus norvegicus (Rat) Rat MEVMNLIEQPIKVTEWQQTYTYDSGIHSGVNTCVPSVSSKGLLDEDDTCGRQYTLKKTTTYTQGVPQSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQTTNLQRLAEPSQLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQESKLIILANGGPQGLVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLENVLKILVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYADDMDATYRPMYSSDVPLDPLDMHMDMDGDYPMDTYSDGLRPPYPAADHMLA q02487-2 Desmocollin-2 DGIII DSC2 CDHF2 DSC3 Cadherin family member 2 Desmocollin-3 Desmosomal glycoprotein II Desmosomal glycoprotein III protein protein human Homo sapiens Human MEAARPSGSWNGALCRLLLLTLAILIFASDACKNVTLHVPSKLDAEKLVGRVNLKECFTAANLIHSSDPDFQILEDGSVYTTNTILLSSEKRSFTILLSNTENQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPIPCSMLENSLGPFPLFLQQVQSDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFEIIAFATTPDGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTMHTRLKYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFGLQTTSTCIINIDDVNDHLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRANYTILKGNENGNFKIVTDAKTNEGVLCVVKPLNYEEKQQMILQIGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTSNGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCTGTLGIILQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAINDTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVDPRIGGGGVQLGKWAILAILLGIALLFCILFTLVCGASGTSKQPKVIPDDLAQQNLIVSNTEAPGDDKVYSANGFTTQTVGASAQGVCGTVGSGIKNGGQETIEMVKGGHQTSESCRGAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEESIRGHTLIKN cxa1_human Gap junction alpha-1 protein GJAL Connexin-43 Gap junction 43 kDa heart protein GJA1 protein protein human Homo sapiens Human MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI desp_human Desmoplakin DSP 250/210 kDa paraneoplastic pemphigus antigen protein protein human Homo sapiens Human MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRRGVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRSRELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHRGIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSREIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQHPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSIRKKYPCDKNMPLQHLLEQIKELEKEREKILEYKRQVQNLVNKSKKIVQLKPRNPDYRSNKPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNPLAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDYMKTIADLELHYQEFIRNSQGSEMFGDDDKRKIQSQFTDAQKHYQTLVIQLPGYPQHQTVTTTEITHHGTCQDVNHNKVIETNRENDKQETWMLMELQKIRRQIEHCEGRMTLKNLPLADQGSSHHITVKINELKSVQNDSQAIAEVLNQLKDMLANFRGSEKYCYLQNEVFGLFQKLENINGVTDGYLNSLCTVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLKKIKNDLNLKKSLLATMKTELQKAQQIHSQTSQQYPLYDLDLGKFGEKVTQLTDRWQRIDKQIDFRLWDLEKQIKQLRNYRDNYQAFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQKNLHSEISGKRDKSEEVQKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMIQSPSGVILQEAADVHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDANSENCNKNKFLDQNLQKYQAECSQFKAKLASLEELKRQAELDGKSAKQNLDKCYGQIKELNEKITRLTYEIEDEKRRRKSVEDRFDQQKNDYDQLQKARQCEKENLGWQKLESEKAIKEKEYEIERLRVLLQEEGTRKREYENELAKVRNHYNEEMSNLRNKYETEINITKTTIKEISMQKEDDSKNLRNQLDRLSRENRDLKDEIVRLNDSILQATEQRRRAEENALQQKACGSEIMQKKQHLEIELKQVMQQRSEDNARHKQSLEEAAKTIQDKNKEIERLKAEFQEEAKRRWEYENELSKVRNNYDEEIISLKNQFETEINITKTTIHQLTMQKEEDTSGYRAQIDNLTRENRSLSEEIKRLKNTLTQTTENLRRVEEDIQQQKATGSEVSQRKQQLEVELRQVTQMRTEESVRYKQSLDDAAKTIQDKNKEIERLKQLIDKETNDRKCLEDENARLQRVQYDLQKANSSATETINKLKVQEQELTRLRIDYERVSQERTVKDQDITRFQNSLKELQLQKQKVEEELNRLKRTASEDSCKRKKLEEELEGMRRSLKEQAIKITNLTQQLEQASIVKKRSEDDLRQQRDVLDGHLREKQRTQEELRRLSSEVEALRRQLLQEQESVKQAHLRNEHFQKAIEDKSRSLNESKIEIERLQSLTENLTKEHLMLEEELRNLRLEYDDLRRGRSEADSDKNATILELRSQLQISNNRTLELQGLINDLQRERENLRQEIEKFQKQALEASNRIQESKNQCTQVVQERESLLVKIKVLEQDKARLQRLEDELNRAKSTLEAETRVKQRLECEKQQIQNDLNQWKTQYSRKEEAIRKIESEREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSTRETQSQLETERSRYQREIDKLRQRPYGSHRETQTECEWTVDTSKLVFDGLRKKVTAMQLYECQLIDKTTLDKLLKGKKSVEEVASEIQPFLRGAGSIAGASASPKEKYSLVEAKRKKLISPESTVMLLEAQAATGGIIDPHRNEKLTVDSAIARDLIDFDDRQQIYAAEKAITGFDDPFSGKTVSVSEAIKKNLIDRETGMRLLEAQIASGGVVDPVNSVFLPKDVALARGLIDRDLYRSLNDPRDSQKNFVDPVTKKKVSYVQLKERCRIEPHTGLLLLSVQKRSMSFQGIRQPVTVTELVDSGILRPSTVNELESGQISYDEVGERIKDFLQGSSCIAGIYNETTKQKLGIYEAMKIGLVRPGTALELLEAQAATGFIVDPVSNLRLPVEEAYKRGLVGIEFKEKLLSAERAVTGYNDPETGNIISLFQAMNKELIEKGHGIRLLEAQIATGGIIDPKESHRLPVDIAYKRGYFNEELSEILSDPSDDTKGFFDPNTEENLTYLQLKERCIKDEETGLCLLPLKEKKKQVQTSQKNTLRKRRVVIVDPETNKEMSVQEAYKKGLIDYETFKELCEQECEWEEITITGSDGSTRVVLVDRKTGSQYDIQDAIDKGLVDRKFFDQYRSGSLSLTQFADMISLKNGVGTSSSMGSGVSDDVFSSSRHESVSKISTISSVRNLTIRSSSFSDTLEESSPIAAIFDTENLEKISITEGIERGIVDSITGQRLLEAQACTGGIIHPTTGQKLSLQDAVSQGVIDQDMATRLKPAQKAFIGFEGVKGKKKMSAAEAVKEKWLPYEAGQRFLEFQYLTGGLVDPEVHGRISTEEAIRKGFIDGRAAQRLQDTSSYAKILTCPKTKLKISYKDAINRSMVEDITGLRLLEAASVSSKGLPSPYNMSSAPGSRSGSRSGSRSGSRSGSRSGSRRGSFDATGNSSYSYSYSFSSSSIGH dsc2_human Desmocollin-2 DSC2 CDHF2 DSC3 Cadherin family member 2 Desmocollin-3 Desmosomal glycoprotein II Desmosomal glycoprotein III protein protein human Homo sapiens Human MEAARPSGSWNGALCRLLLLTLAILIFASDACKNVTLHVPSKLDAEKLVGRVNLKECFTAANLIHSSDPDFQILEDGSVYTTNTILLSSEKRSFTILLSNTENQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPIPCSMLENSLGPFPLFLQQVQSDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFEIIAFATTPDGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTMHTRLKYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFGLQTTSTCIINIDDVNDHLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRANYTILKGNENGNFKIVTDAKTNEGVLCVVKPLNYEEKQQMILQIGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTSNGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCTGTLGIILQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAINDTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVDPRIGGGGVQLGKWAILAILLGIALLFCILFTLVCGASGTSKQPKVIPDDLAQQNLIVSNTEAPGDDKVYSANGFTTQTVGASAQGVCGTVGSGIKNGGQETIEMVKGGHQTSESCRGAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEKVYLCNQDENHKHAQDYVLTYNYEGRGSVAGSVGCCSERQEEDGLEFLDNLEPKFRTLAEACMKR dsg2_human Desmoglein-2 CDHF5 HDGC Cadherin family member 5 DSG2 protein protein human Homo sapiens Human MARSPGRAYALLLLLICFNVGSGLHLQVLSTRNENKLLPKHPHLVRQKRAWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVFVGSVEELSAAHTLVMKINATDADEPNTLNSKISYRIVSLEPAYPPVFYLNKDTGEIYTTSVTLDREEHSSYTLTVEARDGNGEVTDKPVKQAQVQIRILDVNDNIPVVENKVLEGMVEENQVNVEVTRIKVFDADEIGSDNWLANFTFASGNEGGYFHIETDAQTNEGIVTLIKEVDYEEMKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYVGLGPAAIALMILAFLLLLLVPLLLLMCHCGKGAKGFTPIPGTIEMLHPWNNEGAPPEDKVVPSFLPVDQGGSLVGRNGVGGMAKEATMKGSSSASIVKGQHEMSEMDGRWEEHRSLLSGRATQFTGATGAIMTTETTKTARATGASRDMAGAQAAAVALNEEFLRNYFTDKAASYTEEDENHTAKDCLLVYSQEETESLNASIGCCSFIEGELDDRFLDDLGLKFKTLAEVCLGQKIDINKEIEQRQKPATETSMNTASHSLCEQTMVNSENTYSSGSSFPVPKSLQEANAEKVTQEIVTERSVSSRQAQKVATPLPDPMASRNVIATETSYVTGSTMPPTTVILGPSQPQSLIVTERVYAPASTLVDQPYANEGTVVVTERVIQPHGGGSNPLEGTQHLQDVPYVMVRERESFLAPSSGVQPTLAMPNIAVGQNVTVTERVLAPASTLQSSYQIPTENSMTARNTTVSGAGVPGPLPDFGLEESGHSNSTITTSSTRVTKHSTVQHSYS dsc2-jup-4 1427799 n/a 1427806 unspecified role unspecified role bait bait n/a 1427807 unspecified role unspecified role prey prey physical association physical association false false false 5C -wt There was impaired binding of the DSC2a mutant full-length protein to plakoglobin. jup-dsc2 1427799 n/a 1427806 unspecified role unspecified role prey prey Q02487-1:p.Asp852_Val854delinsThrMetSer mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1427808 unspecified role unspecified role bait bait physical association physical association false false false 6d mut There was impaired binding of the DSC2a mutant full-length protein. There appears to be weak binding between Cx43 and mutant DSC2a, in spite of the text stating there is no binding. gja1-dsc2 1427799 n/a 1427806 unspecified role unspecified role prey prey n/a 1427808 unspecified role unspecified role bait bait physical association physical association false false false 6D There was impaired binding of the DSC2a mutant full-length protein. dsc2-gja1-6 1427799 n/a 1427807 unspecified role unspecified role prey prey n/a 1427806 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q02487-1:p.Asp852_Val854delinsThrMetSer mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5C mut There was impaired binding of the DSC2a mutant full-length protein. dsc2-jup-1 1427800 n/a 1427809 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] n/a 1427806 unspecified role unspecified role bait bait cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1427810 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] association association false false false 5B -wt dsc2-jup-2 1427800 n/a 1427809 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] n/a 1427806 unspecified role unspecified role bait bait cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q02487-1:p.Asp852_Val854delinsThrMetSer mutation mutation certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 5 B -mut DSC2b (and the mutant) bound only to PKP2 (Figure 5B). dsc2-pkp2 1427800 n/a 1427811 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1427809 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] physical association physical association false false false 5B-2b DSC2b (and the mutant) bound only to PKP2 (Figure 5B). dsc2-jup 1427801 n/a 1427810 unspecified role unspecified role neutral component neutral component n/a 1427806 unspecified role unspecified role neutral component neutral component Q02487-1:p.Asp852_Val854delinsThrMetSer mutation mutation certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 4B dsc2-jup-5 1427801 n/a 1427806 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1427810 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4Bi dsc2-gja1 1427802 n/a 1427806 unspecified role unspecified role bait bait cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1427812 unspecified role unspecified role prey prey n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 6A -wt dsc2-gja1-7 1427802 n/a 1427813 unspecified role unspecified role prey prey ntp_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1427806 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytoplasmic_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Supp 5A dsc2-gja1-2 1427803 n/a 1427806 unspecified role unspecified role bait bait cytoplasmic_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1427808 unspecified role unspecified role prey prey physical association physical association false false false 6B DSC2a WT bound to endogenous Cx43, and again the mutant DSC2a protein did not bind (Figure 6B) dsc2-gja1-3 1427803 n/a 1427808 unspecified role unspecified role prey prey n/a 1427811 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false cytoplasmic_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 6B -wt The text states that:- "DSC2a WT bound to endogenous Cx43, and again the mutant DSC2a protein and DSC2b did not bind (Figure 6B)." However a weak band is clearly visible for Dsc2b. dsc2-gja1-4 1427804 n/a 1427812 unspecified role unspecified role prey prey cytoplasmic_c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 n/a 1427806 unspecified role unspecified role bait bait cytoplasmic_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 6C dsg2-dsc2 1427805 n/a 1427814 unspecified role unspecified role prey prey region_including_extracellular_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n/a 1427815 unspecified role unspecified role bait bait c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp 4B, Supp 5B. The A517V mutant shows normal binding to DSC2. Binding to DSG2 by mutant DSC2a (mediated by the extracellular portion) was preserved (Figure S5B).