bhf-ucl l_pez_lira-2006-1 The role of beta2-glycoprotein I (beta2GPI) in the activation of plasminogen. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa tag visualisation tag visualisation López-Lira F., Rosales-León L., Martínez V.M., Ruiz Ordaz B.H. Cardiac - Interactions involving cardiac related proteins Biotinylated Glu-Plasminogen was incubated with the immobilised Beta2-GPI. The plate was washed to get rid of unbound Glu-Plasminogen and then incubated with streptavidin-peroxidase conjugate. The ranges of K5sp are not given in the publication and can not be traced bhro@servidor.unam.mx Biochim. Biophys. Acta (0006-3002) 2006 Only protein-protein interactions imex curation apoh_human Beta-2-glycoprotein 1 APOH B2G1 Beta-2-glycoprotein I Apolipoprotein H Activated protein C-binding protein APC inhibitor Anticardiolipin cofactor protein protein human Homo sapiens Human MISPVLILFSSFLCHVAIAGRTCPKPDDLPFSTVVPLKTFYEPGEEITYSCKPGYVSRGGMRKFICPLTGLWPINTLKCTPRVCPFAGILENGAVRYTTFEYPNTISFSCNTGFYLNGADSAKCTEEGKWSPELPVCAPIICPPPSIPTFATLRVYKPSAGNNSLYRDTAVFECLPQHAMFGNDTITCTTHGNWTKLPECREVKCPFPSRPDNGFVNYPAKPTLYYKDKATFGCHDGYSLDGPEEIECTKLGNWSAMPSCKASCKVPVKKATVVYQGERVKIQEKFKNGMLHGDKVSFFCKNKEKKCSYTEDAQCIDGTIEVPKCFKEHSSLAFWKTDASDVKPC plmn_human Plasminogen PLG protein protein human Homo sapiens Human MEHKEVVLLLLLFLKSGQGEPLDDYVNTQGASLFSVTKKQLGAGSIEECAAKCEEDEEFTCRAFQYHSKEQQCVIMAENRKSSIIIRMRDVVLFEKKVYLSECKTGNGKNYRGTMSKTKNGITCQKWSSTSPHRPRFSPATHPSEGLEENYCRNPDNDPQGPWCYTTDPEKRYDYCDILECEEECMHCSGENYDGKISKTMSGLECQAWDSQSPHAHGYIPSKFPNKNLKKNYCRNPDRELRPWCFTTDPNKRWELCDIPRCTTPPPSSGPTYQCLKGTGENYRGNVAVTVSGHTCQHWSAQTPHTHNRTPENFPCKNLDENYCRNPDGKRAPWCHTTNSQVRWEYCKIPSCDSSPVSTEQLAPTAPPELTPVVQDCYHGDGQSYRGTSSTTTTGKKCQSWSSMTPHRHQKTPENYPNAGLTMNYCRNPDADKGPWCFTTDPSVRWEYCNLKKCSGTEASVVAPPPVVLLPDVETPSEEDCMFGNGKGYRGKRATTVTGTPCQDWAAQEPHRHSIFTPETNPRAGLEKNYCRNPDGDVGGPWCYTTNPRKLYDYCDVPQCAAPSFDCGKPQVEPKKCPGRVVGGCVAHPHSWPWQVSLRTRFGMHFCGGTLISPEWVLTAAHCLEKSPRPSSYKVILGAHQEVNLEPHVQEIEVSRLFLEPTRKDIALLKLSSPAVITDKVIPACLPSPNYVVADRTECFITGWGETQGTFGAGLLKEAQLPVIENKVCNRYEFLNGRVQSTELCAGHLAGGTDSCQGDSGGPLVCFEKDKYILQGVTSWGLGCARPNKPGVYVRVSRFVTWIEGVMRNN apoh-plg-2 1546538 n/a 1546539 unspecified role unspecified role bait bait human-serum Homo sapiens serum Serum n/a 1546540 unspecified role unspecified role prey prey k5sp domain required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-serum Homo sapiens serum Serum direct interaction direct interaction false false false Fig. 1, 2A, B, C EACA (Epsilon Amino Caproic Acid), NaCl apoh-plg-3 1546538 n/a 1546540 competitor competitor competitor unspecified role unspecified role k5 sp domain required to bind necessary binding region required to bind range range range range false Biotinylated Glu-Plasminogen and miniplasminogen or K5-sp were incubated with the immobilised β2GPI for a binding competition assay n/a 1546539 unspecified role unspecified role bait bait human-serum Homo sapiens serum Serum n/a 1546540 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-serum Homo sapiens serum Serum direct interaction direct interaction false false false Fig.2B