IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ dardenne-2016-2 human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu Cancer cell 2016 imex curation Only protein-protein interactions dardenne-2016-1 human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu Cancer cell 2016 imex curation Only protein-protein interactions dardenne-2016-3 human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Dardenne E., Beltran H., Benelli M., Gayvert K., Berger A., Puca L., Cyrta J., Sboner A., Noorzad Z., MacDonald T., Cheung C., Yuen KS., Gao D., Chen Y., Eilers M., Mosquera JM., Robinson BD., Elemento O., Rubin MA., Demichelis F., Rickman DS. Cancer - Interactions investigated in the context of cancer dsr2005@med.cornell.edu Cancer cell 2016 imex curation Only protein-protein interactions klk3_human_gene Human kallikrein-related peptidase 3 (KLK3) (PSA) gene gene human Homo sapiens Human mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC tmps2_human_gene Human TMPS2/TMPRSS2 gene gene gene human Homo sapiens Human ndrg1_human_gene Human NDRG1 gene gene gene human Homo sapiens Human ezh2_human Histone-lysine N-methyltransferase EZH2 KMT6 Enhancer of zeste homolog 2 ENX-1 Lysine N-methyltransferase 6 EZH2 protein protein human Homo sapiens Human MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP sox17_human_gene Transcription factor SOX-17 SOX17 gene gene human Homo sapiens Human ndka_human_gene Nucleoside diphosphate kinase A NME1 NDPKA NM23 gene gene human Homo sapiens Human fkbp5_human_gene Human FKBP5 gene gene gene human Homo sapiens Human cadh5_human_gene Cadherin-5 CDH5 gene gene human Homo sapiens Human andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS suz12_human Polycomb protein SUZ12 CHET9 JJAZ1 KIAA0160 SUZ12 Suppressor of zeste 12 protein homolog Joined to JAZF1 protein Chromatin precipitated E2F target 9 protein protein protein human Homo sapiens Human MAPQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL max_human Protein max MAX BHLHD4 Class D basic helix-loop-helix protein 4 Myc-associated factor X protein protein human Homo sapiens Human MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS mycn-klk3_human_gene-1 2865083 N/A 2865086 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865087 unspecified role unspecified role bait bait association association false false false figure 4D CHEBI:16330 increases the binding of P04198 to ENSG00000142515 DHT: CHEBI:16330 mycn-tmps2_human_gene-1 2865083 N/A 2865088 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865087 unspecified role unspecified role bait bait association association false false false figure 4Di CHEBI:16330 increases the binding of P04198 to ENSG00000184012 DHT: CHEBI:16330 mycn-tmps2_human_gene-2 2865083 N/A 2865089 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865087 unspecified role unspecified role bait bait association association false false false figure 4Dii CHEBI:16330 increases the binding of P04198 to ENSG00000104419 DHT: CHEBI:16330 asn235lys-1 2865083 N/A 2865086 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865090 unspecified role unspecified role bait bait association association false false false 7D-1 N-myc over-expression ezh2-klk3_human_gene-1 2865083 N/A 2865091 unspecified role unspecified role prey prey N/A 2865090 unspecified role unspecified role bait bait association association false false false 7D-2 N-myc over-expression ezh2-klk3_human_gene-2 2865083 N/A 2865092 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865090 unspecified role unspecified role bait bait association association false false false 7D-3 ezh2-klk3_human_gene-3 2865083 N/A 2865093 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865090 unspecified role unspecified role bait bait association association false false false 7D-4 N-myc over-expression ezh2-fkbp5_human_gene-1 2865083 N/A 2865088 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865090 unspecified role unspecified role bait bait association association false false false 7D-5 N-myc over-expression ezh2-tmps2_human_gene-1 2865083 N/A 2865089 unspecified role unspecified role prey prey N/A 2865090 unspecified role unspecified role bait bait association association false false false 7D-6 N-myc over-expression mycn-tmps2_human_gene-3 2865083 N/A 2865094 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865087 unspecified role unspecified role bait bait association association false false false Supp fig 4E CHEBI:16330 increases the binding of P04198 to ENSG00000184012 DHT: CHEBI:16330 ezh2-klk3_human_gene-4 2865083 N/A 2865086 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865095 unspecified role unspecified role bait bait association association false false false Supp 4D N-myc over-expression (small effect) ezh2-tmps2_human_gene-2 2865083 N/A 2865088 unspecified role unspecified role prey prey enhancer_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 2865095 unspecified role unspecified role bait bait association association false false false Supp 4D-2 N-myc over-expression (slight effect) ezh2-ndrg1_human_gene-1 2865083 N/A 2865089 unspecified role unspecified role prey prey N/A 2865095 unspecified role unspecified role bait bait association association false false false Supp 4D-3 N-myc over-expression d8ct6yud5-1 2865084 N/A 2865095 unspecified role unspecified role prey prey N/A 2865096 unspecified role unspecified role prey prey N/A 2865087 unspecified role unspecified role bait bait association association false false false figure 4D- small inset image under "KLK3 Locus" mycn-ar-1 2865084 N/A 2865090 unspecified role unspecified role bait bait N/A 2865095 unspecified role unspecified role prey prey N/A 2865097 unspecified role unspecified role prey prey N/A 2865087 unspecified role unspecified role prey prey association association false false false 7B-1 An EZH2 inhibitor GSK126 reduces the interaction with N-Myc and AR (but not SUZ12) An EZH2 inhibitor GSK343 reduces the interaction with N-Myc and AR (but not SUZ12) ezh2-ar-1 2865084 N/A 2865098 unspecified role unspecified role prey prey N/A 2865090 unspecified role unspecified role prey prey N/A 2865095 unspecified role unspecified role prey prey N/A 2865097 unspecified role unspecified role prey prey N/A 2865087 unspecified role unspecified role bait bait association association false false false 7B-2 An EZH2 inhibitor GSK126 reduces the interaction with N-Myc and AR (but not SUZ12 or MAX) An EZH2 inhibitor GSK343 reduces the interaction with N-Myc and AR (but not SUZ12 or MAX) mycn-ar-2 2865084 N/A 2865098 unspecified role unspecified role prey prey N/A 2865090 unspecified role unspecified role prey prey N/A 2865095 unspecified role unspecified role prey prey N/A 2865087 unspecified role unspecified role bait bait association association false false false 7B-3 (Supp7C) An EZH2 mutant of unknown origin, lacking the SET domain sequence (aas 612-727 in the Human protein) still interacted. mycn-ar-3 2865084 N/A 2865098 unspecified role unspecified role prey prey N/A 2865087 unspecified role unspecified role bait bait association association false false false 7B-4 In the presence of EZH2 siRNA, only MAX was detected. mycn-max-3 2865084 N/A 2865096 unspecified role unspecified role prey prey N/A 2865098 unspecified role unspecified role prey prey N/A 2865087 unspecified role unspecified role bait bait association association false false false 8B MLN8237:An allosteric Aurora-A Inhibitor reduced the interaction of N-Myc with Aurora A but not MAX. r9rd9-1 2865085 N/A 2865096 unspecified role unspecified role neutral component neutral component N/A 2865087 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 8A MLN8237;an Allosteric Aurora-A Inhibitor