IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ vo-2016-1 mouse-medulloblastoma Mouse medulloblastoma medulloblastoma Medulloblastoma ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer The authors confirm that Supplementary Figure 3F results were obtained from G3-MB cells. G3-MB cells; Group3 is a subgroup of (paediatric) medulloblastoma, here induced by c-Myc (Myc) overexpression in granule neuron progenitor cells (GNPs). (In humans Group 3 tumours are often associated with Myc ampification. They are associated with the worst prognosis of all the subgroups.) Granule neuron progenitors (GNPs) were infected with mouse stem cell virus (MSCV)-based retroviruses; these viruses either express RFP/GFP alone or co-express mouse Myc or Mycn cDNA. Infected GNP-enriched cerebellar cells were transplanted into the cortices or cerebellum of nude recipient mice. (Kawauchi et al.,) Extracted tumour cells were also grown in vitro as tumorspheres. HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Cancer cell 2016 imex curation Only protein-protein interactions vo-2016-4 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Cancer cell 2016 imex curation Only protein-protein interactions vo-2016-5 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Cancer cell 2016 imex curation Only protein-protein interactions vo-2016-7 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Cancer cell 2016 imex curation Only protein-protein interactions vo-2016-8 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Cancer cell 2016 imex curation Only protein-protein interactions vo-2016-2 mouse-neuronal_precursor_cells(stem cells) Mouse Neuronal precursor cells/neural stem progenitor cells neuronal_precursor (stem cells) Neuronal Precursor Cells/neural stem progenitor cells ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Cancer cell 2016 imex curation Only protein-protein interactions vo-2016-6 mouse-medulloblastoma Mouse medulloblastoma medulloblastoma Medulloblastoma ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Sonic Hedgehog (SHH) medulloblastoma; here induced by MycN overexpression in granule neuron progenitor cells (GNPs). Granule neuron progenitors (GNPs) were infected with mouse stem cell virus (MSCV)-based retroviruses; these viruses either express RFP/GFP alone or co-express mouse Myc or Mycn cDNA. Infected GNP-enriched cerebellar cells were transplanted into the cortices or cerebellum of nude recipient mice. (Kawauchi et al.,) Extracted tumour cells were also grown in vitro as tumorspheres. HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Cancer cell 2016 imex curation Only protein-protein interactions myc_mouse Myc proto-oncogene protein Proto-oncogene c-Myc Transcription factor p64 Myc protein protein mouse Mus musculus Mouse MPLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA noct_mouse_gene Nocturnin Noct Ccr4 Ccrn4l Noc gene gene mouse Mus musculus Mouse zbt17_mouse Zinc finger and BTB domain-containing protein 17 Zbtb17 Zfp100 Znf151 LP-1 Polyomavirus late initiator promoter-binding protein Zinc finger protein 100 Zinc finger protein 151 Zinc finger protein Z13 protein protein mouse Mus musculus Mouse MDFPQHSQRVLEQLNQQRQLGLLCDCTFVVDGVDFKAHKAVLAACSEYFKMLFVDQKDVVHLDISNAAGLGQVLEFMYTAKLSLSPENVDDVLAVASFLQMQDIVTACHTLKSLAEPSSTTGESADASAVEGGDKRAKDEKAAATMLSRLDQARGSSSTGPGRELKEERGGQAESASSGAEQTEKADAPREPPPVELKPDPTSSMAAAEAEALSESSEQEMEVEPASKGEDGQEEEGAGPATVKEEGMHLDNGEPPEENEESAGTDSGQELGMEGQNLRSGTYGDRTESKAYGSIIHKCEDCGKEFTHTGNFKRHIRIHTGEKPFSCRECSKAFSDPAACKAHEKTHSPLKPYGCEECGKSYRLISLLNLHKKRHSGEARYRCGDCGKLFTTSGNLKRHQLVHSGQKPYQCDYCGRSFSDPTSKMRHLETHDTDKEHKCPHCDKKFNQVGNLKAHLKIHIADGPLKCRECGKQFTTSGNLKRHLRIHSGEKPYVCTHCQRQFADPGALQRHVRIHTGEKPCQCVICGKAFTQASSLIAHVRQHTGEKPYVCERCGKRFVQSSQLANHIRHHDNIRPHKCSVCSKAFVNVGDLSKHIIIHTGEKPYLCDKCGRGFNRVDNLRSHVKTVHQGKAGIKILEPEEGGEVSVVTVDDMVTLATEALAATAVTQLTVVPVGAAVTADETEVLKAEISKAVKQVQEEDPNTHILYACDSCGDKFLDANSLAQHVRIHTAQALVMFQTDADFYQQYGPGSTWPAGQMLQAGELVFRPRDGTEGQPTLAESPPTAPDCLPPAE atoh1_mouse_gene Protein atonal homolog 1 gene gene mouse Mus musculus Mouse egr1_mouse_gene Mouse EGR1 gene gene gene mouse Mus musculus Mouse foxg1_mouse_gene Forkhead box protein G1 Foxg1 Fkhl1 Foxg1b Hfhbf1 gene gene mouse Mus musculus Mouse vps72_mouse_gene Vacuolar protein sorting-associated protein 72 homolog Vps72 Tcfl1 Yl1 gene gene mouse Mus musculus Mouse dcor_mouse_gene Ornithine decarboxylase Odc1 Odc gene gene mouse Mus musculus Mouse ck2n1_mouse_gene Calcium/calmodulin-dependent protein kinase II inhibitor 1 Camk2n1 gene gene mouse Mus musculus Mouse pcbp1_mouse_gene Poly(rC)-binding protein 1 Pcbp1 gene gene mouse Mus musculus Mouse mouse_ccnd1_gene Ccnd1/G1/S-specific cyclin-D1 Mouse Gene gene gene mouse Mus musculus Mouse tmm11_mouse_gene Transmembrane protein 11, mitochondrial Tmem11 gene gene mouse Mus musculus Mouse zbt17_human Zinc finger and BTB domain-containing protein 17 ZBTB17 MIZ1 Zinc finger protein 60 Zinc finger protein 151 Myc-interacting zinc finger protein 1 ZNF151 ZNF60 protein protein human Homo sapiens Human MDFPQHSQHVLEQLNQQRQLGLLCDCTFVVDGVHFKAHKAVLAACSEYFKMLFVDQKDVVHLDISNAAGLGQVLEFMYTAKLSLSPENVDDVLAVATFLQMQDIITACHALKSLAEPATSPGGNAEALATEGGDKRAKEEKVATSTLSRLEQAGRSTPIGPSRDLKEERGGQAQSAASGAEQTEKADAPREPPPVELKPDPTSGMAAAEAEAALSESSEQEMEVEPARKGEEEQKEQEEQEEEGAGPAEVKEEGSQLENGEAPEENENEESAGTDSGQELGSEARGLRSGTYGDRTESKAYGSVIHKCEDCGKEFTHTGNFKRHIRIHTGEKPFSCRECSKAFSDPAACKAHEKTHSPLKPYGCEECGKSYRLISLLNLHKKRHSGEARYRCEDCGKLFTTSGNLKRHQLVHSGEKPYQCDYCGRSFSDPTSKMRHLETHDTDKEHKCPHCDKKFNQVGNLKAHLKIHIADGPLKCRECGKQFTTSGNLKRHLRIHSGEKPYVCIHCQRQFADPGALQRHVRIHTGEKPCQCVMCGKAFTQASSLIAHVRQHTGEKPYVCERCGKRFVQSSQLANHIRHHDNIRPHKCSVCSKAFVNVGDLSKHIIIHTGEKPYLCDKCGRGFNRVDNLRSHVKTVHQGKAGIKILEPEEGSEVSVVTVDDMVTLATEALAATAVTQLTVVPVGAAVTADETEVLKAEISKAVKQVQEEDPNTHILYACDSCGDKFLDANSLAQHVRIHTAQALVMFQTDADFYQQYGPGGTWPAGQVLQAGELVFRPRDGAEGQPALAETSPTAPECPPPAE myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC myc-noct-1 1904844 N/A 1904851 unspecified role unspecified role bait bait N/A 1904852 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3B zbtb17-noct-2 1904844 N/A 1904853 unspecified role unspecified role bait bait N/A 1904852 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3B-2 myc-atoh1_mouse_gene-1 1904844 N/A 1904851 unspecified role unspecified role bait bait N/A 1904854 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4E zbtb17-atoh1_mouse_gene-1 1904844 N/A 1904853 unspecified role unspecified role bait bait N/A 1904854 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4E-2 zbtb17-egr1_mouse_gene-1 1904844 N/A 1904853 unspecified role unspecified role bait bait N/A 1904855 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5F-1 zbtb17-foxg1-1 1904844 N/A 1904853 unspecified role unspecified role bait bait N/A 1904856 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5F-2 zbtb17-vps72-1 1904844 N/A 1904853 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false N/A 1904857 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5F-3, Supp 3C/F myc-vps72-1 1904844 N/A 1904851 unspecified role unspecified role bait bait N/A 1904857 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 3C myc-odc1-1 1904844 N/A 1904851 unspecified role unspecified role bait bait N/A 1904858 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 3Ci myc-odc1-2 1904844 N/A 1904858 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1904853 unspecified role unspecified role bait bait association association false false false Supp 3Cii myc-camk2n1-1 1904844 N/A 1904851 unspecified role unspecified role bait bait N/A 1904859 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 3Ciii zbtb17-camk2n1-1 1904844 N/A 1904853 unspecified role unspecified role bait bait N/A 1904859 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 3Civ zbtb17-vps72-3 1904844 N/A 1904853 unspecified role unspecified role bait bait poz_domain binding region binding-associated region binding region range range range range false N/A 1904860 unspecified role unspecified role prey prey association association false false false Supp 3F zbtb17-mouse_ccnd1_gene-1 1904844 N/A 1904853 unspecified role unspecified role bait bait poz_domain binding region binding-associated region binding region range range range range false N/A 1904861 unspecified role unspecified role prey prey association association false false false Supp 3F-1 zbtb17-mouse_ccnd1_gene-2 1904844 N/A 1904853 unspecified role unspecified role bait bait poz_domain binding region binding-associated region binding region range range range range false N/A 1904862 unspecified role unspecified role prey prey association association false false false Supp 3F-2 23541-1 1904845 N/A 1904863 unspecified role unspecified role prey prey N/A 1904864 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5D, Supp 3E/5E The Miz1_delta POZ protein still binds to Myc. mycn-zbtb17-2 1904845 N/A 1904863 unspecified role unspecified role prey prey N/A 1904865 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P04198:p.Val421Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5Di/Supp 5A/5D mycn-zbtb17-1 1904846 N/A 1904863 unspecified role unspecified role bait bait N/A 1904865 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5E zbtb17-myc-2 1904846 N/A 1904863 unspecified role unspecified role bait bait N/A 1904864 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5E zbtb17-myc-3 1904847 N/A 1904863 unspecified role unspecified role bait bait N/A 1904864 unspecified role unspecified role prey prey P01106:p.Val394Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Supp 3D myc-zbtb17-4 1904847 N/A 1904864 unspecified role unspecified role bait bait P01106:p.Val394Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false N/A 1904863 unspecified role unspecified role prey prey physical association physical association false false false Supp 3Di zbtb17-myc-4 1904848 N/A 1904864 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 1904863 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp 5B zbtb17-mycn-1 1904848 N/A 1904865 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 1904863 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp 5Bi Very weak interaction. zbtb17-noct-1 1904849 N/A 1904853 unspecified role unspecified role bait bait N/A 1904852 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3B-2 zbtb17-vps72-2 1904850 N/A 1904853 unspecified role unspecified role bait bait N/A 1904857 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5F-4