IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xu-2015-8 human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Cancer cell 2015 imex curation The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. Only protein-protein interactions imex curation xu-2015-11 human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation xu-2015-3 human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Cancer cell 2015 imex curation The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. Only protein-protein interactions imex curation xu-2015-4 human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Cancer cell 2015 imex curation The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. Only protein-protein interactions imex curation xu-2015-5 human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Cancer cell 2015 imex curation Only protein-protein interactions imex curation xu-2015-6 human-mcf12a human-mcf12a-Human Immortalised non-tumorigenic human mammary epithelial cells Human mcf12a MCF12A Human Immortalised non-tumorigenic human mammary epithelial cells http://www.lgcstandards-atcc.org/products/all/CRL-10782.aspx?geo_country=gb#generalinformation anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. dyu@mdanderson.org Cancer cell 2015 imex curation Only protein-protein interactions imex curation xu-2015-7 human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Cancer cell 2015 imex curation The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. Only protein-protein interactions imex curation xu-2015-9 human-mcf12a human-mcf12a-Human Immortalised non-tumorigenic human mammary epithelial cells Human mcf12a MCF12A Human Immortalised non-tumorigenic human mammary epithelial cells http://www.lgcstandards-atcc.org/products/all/CRL-10782.aspx?geo_country=gb#generalinformation anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Cancer cell 2015 imex curation The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. Only protein-protein interactions imex curation xu-2015-10 human-mcf12a human-mcf12a-Human Immortalised non-tumorigenic human mammary epithelial cells Human mcf12a MCF12A Human Immortalised non-tumorigenic human mammary epithelial cells http://www.lgcstandards-atcc.org/products/all/CRL-10782.aspx?geo_country=gb#generalinformation confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Xu J., Acharya S., Sahin O., Zhang Q., Saito Y., Yao J., Wang H., Li P., Zhang L., Lowery FJ., Kuo WL., Xiao Y., Ensor J., Sahin AA., Zhang XH., Hung MC., Zhang JD., Yu D. Cancer - Interactions investigated in the context of cancer dyu@mdanderson.org Cancer cell 2015 imex curation The authors have confirmed that these constructs are of Human origin:HA-14-3-3-zeta, Myc-Gli2, GST-14-3-3-zeta. Only protein-protein interactions imex curation smad3_human Mothers against decapentaplegic homolog 3 SMAD3 MADH3 SMAD family member 3 JV15-2 protein protein human Homo sapiens Human MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS smad2_human Mothers against decapentaplegic homolog 2 SMAD2 MADH2 MADR2 Mad-related protein 2 SMAD family member 2 JV18-1 protein protein human Homo sapiens Human MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS gli2_human Zinc finger protein GLI2 GLI2 THP GLI family zinc finger protein 2 Tax helper protein protein protein human Homo sapiens Human METSASATASEKQEAKSGILEAAGFPDPGKKASPLVVAAAAAAAVAAQGVPQHLLPPFHAPLPIDMRHQEGRYHYEPHSVHGVHGPPALSGSPVISDISLIRLSPHPAGPGESPFNAPHPYVNPHMEHYLRSVHSSPTLSMISAARGLSPADVAQEHLKERGLFGLPAPGTTPSDYYHQMTLVAGHPAPYGDLLMQSGGAASAPHLHDYLNPVDVSRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMALTSINATPTQLSSSSNCLSDTNQNKQSSESAVSSTVNPVAIHKRSKVKTEPEGLRPASPLALTQGQVSGHGSCGCALPLSQEQLADLKEDLDRDDCKQEAEVVIYETNCHWEDCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISPYFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLPAGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDALSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRPPSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGALDEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPMLGGCQLGFGAPSSLNKNNMPVQWNEVSSGTVDALASQVKPPPFPQGNLAVVQQKPAFGQYPGYSPQGLQASPGGLDSTQPHLQPRSGAPSQGIPRVNYMQQLRQPVAGSQCPGMTTTMSPHACYGQVHPQLSPSTISGALNQFPQSCSNMPAKPGHLGHPQQTEVAPDPTTMGNRHRELGVPDSALAGVPPPHPVQSYPQQSHHLAASMSQEGYHQVPSLLPARQPGFMEPQTGPMGVATAGFGLVQPRPPLEPSPTGRHRGVRAVQQQLAYARATGHAMAAMPSSQETAEAVPKGAMGNMGSVPPQPPPQDAGGAPDHSMLYYYGQIHMYEQDGGLENLGSCQVMRSQPPQPQACQDSIQPQPLPSPGVNQVSSTVDSQLLEAPQIDFDAIMDDGDHSSLFSGALSPSLLHSLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFLNMMT fbw1a_human F-box/WD repeat-containing protein 1A BTRC BTRCP FBW1A FBXW1A F-box and WD repeats protein beta-TrCP E3RSIkappaB pIkappaBalpha-E3 receptor subunit Epididymis tissue protein Li 2a protein protein human Homo sapiens Human MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR 1433z_human 14-3-3 protein zeta/delta YWHAZ Protein kinase C inhibitor protein 1 protein protein human Homo sapiens Human MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN smad4_human Mothers against decapentaplegic homolog 4 SMAD4 DPC4 MADH4 SMAD family member 4 Deletion target in pancreatic carcinoma 4 protein protein human Homo sapiens Human MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD yap1_human Transcriptional coactivator YAP1 YAP1 YAP65 Protein yorkie homolog Yes-associated protein YAP65 homolog protein protein human Homo sapiens Human MDPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPAGHQIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL 1433s_human_gene 14-3-3 protein sigma SFN HME1 gene gene human Homo sapiens Human 245twt-1 1800310 N/A 1800319 unspecified role unspecified role prey prey N/A 1800320 unspecified role unspecified role prey prey N/A 1800321 unspecified role unspecified role bait bait association association false false false 5I TGF-beta TGF-beta treatment did not induce Gli2 binding to Smads in 14-3-3-zeta shRNA transfected cells. 14-3-3-zeta shRNA. TGF-beta treatment is shown here to increase the phosphorylation of both Smad 2 and 3 - only no antibody is listed for the dual target antibody in the supplementary "Antibody List", thus it is not possible to determine if one or both Smads were phosphorylated. w57w5-1 1800310 N/A 1800322 unspecified role unspecified role prey prey N/A 1800321 unspecified role unspecified role bait bait physical association physical association false false false Supp Fig 5R Cells were pre-treated with TGF-beta and MG132 - a proteasome inhibitor. Gli2 binding to beta-TrCP was only evident in cells transfected with shRNA to 14-3-3-zeta, but not in cells transfected with control shRNA. gli2-smad2-1 1800310 N/A 1800319 unspecified role unspecified role bait bait N/A 1800321 unspecified role unspecified role prey prey physical association physical association false false false Supp 5T gli2-1 1800310 N/A 1800319 unspecified role unspecified role prey prey N/A 1800321 unspecified role unspecified role prey prey physical association physical association false false false 5F 2454r2rwq-1 1800311 N/A 1800322 unspecified role unspecified role prey prey N/A 1800323 unspecified role unspecified role prey prey N/A 1800321 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5F/G Gli2 binding to beta-Trcp was weaker in control shRNA cells than in 14-3-3-zeta knockout cells. gli2-btrc-1 1800311 N/A 1800322 competitor competitor competitor prey prey N/A gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1800323 unspecified role unspecified role prey prey N/A 1800321 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5G gli2-smad2-2 1800311 N/A 1800324 unspecified role unspecified role prey prey N/A 1800319 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1800320 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1800321 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 5S TGF-beta shCtrl cells were used gli2-btrc-2 1800311 N/A 1800322 competitor competitor competitor prey prey N/A gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1800321 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5Gi smad2-tp53-1 1800312 N/A 1800320 unspecified role unspecified role bait bait N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1800325 unspecified role unspecified role prey prey physical association physical association false false false 1G TGF-beta Transfected 14-3-3-zeta smad2-tp53-2 1800312 N/A 1800320 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1800325 unspecified role unspecified role bait bait physical association physical association false false false Supp 1J TGF-beta Transfected 14-3-3-zeta ywhaz-yap1-4 1800312 N/A 1800326 unspecified role unspecified role bait bait N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false N/A 1800323 unspecified role unspecified role prey prey physical association physical association false false false Supp 2F yap1-sfn-1 1800313 N/A 1800327 unspecified role unspecified role prey prey N/A sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1800326 unspecified role unspecified role bait bait N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false association association false false false 2E 14-3-3-zeta overexpression P46937 increases expression at RNA level of ENSG00000175793 P46937 increases expression at protein level of ENSG00000175793 ywhaz-yap1-1 1800314 N/A 1800326 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false N/A 1800323 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2F ywhaz-yap1-2 1800315 N/A 1800326 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false N/A 1800323 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2G ywhaz-yap1-3 1800316 N/A 1800326 unspecified role unspecified role neutral component neutral component N/A 1800323 unspecified role unspecified role neutral component neutral component N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 2H smad2-tp53-3 1800317 N/A 1800320 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1800325 unspecified role unspecified role bait bait physical association physical association false false false Supp 1J TGF-beta Transfected 14-3-3-zeta yap1-ywhaz-1 1800318 N/A 1800326 unspecified role unspecified role neutral component neutral component N/A 1800323 unspecified role unspecified role neutral component neutral component N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Supp 2G