IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ liu-2014b-12 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-15 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-2 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-3 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-4 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. in vitro In vitro ubiquitinase assay ubiquitinase assay ubiquitinase assay western blot western blot Immuno blot Immuno blot Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-13 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-1 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn human E3 cDNA expression library. Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-5 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-6 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. in vitro In vitro ubiquitinase assay ubiquitinase assay ubiquitinase assay sequence tag sequence tag identification sequence tag Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-9 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. mouse-macrophage Mus musculus macrophage macrophage Macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-10 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. human-h1437 Human H1437 NSCLC, from metastatic site, pleural effusion, adenocarcinoma h1437 Human H1437 NSCLC, from metastatic site, pleural effusion, adenocarcinoma http://www.lgcstandards-atcc.org/products/all/CRL-5872.aspx?geo_country=gb anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-11 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-14 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation liu-2014b-16 Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Liu Z., Chen P., Gao H., Gu Y., Yang J., Peng H., Xu X., Wang H., Yang M., Liu X., Fan L., Chen S., Zhou J., Sun Y., Ruan K., Cheng S., Komatsu M., White E., Li L., Ji H., Finley D., Hu R. Cancer - Interactions investigated in the context of cancer The authors have stated that all these constructs are of Human origin:- HA-OPTN FLAG-HACE1 GST-HACE1 GST-OPTN GFP-OPTN RFP-HACE1 HACE1-Myc HA-Ub FLAG-OPTN MYC and FLAG-p62 and DeltaUBA-p62 constructs Bacterially expressed p62/SQSTM1 Bacterially expressed OPTN Bacterially expressed NBR1 GST-LC3 The authors have stated tha GST-LC3 is Microtubule-associated protein 1 light chain 3 beta. coryhu@sibs.ac.cn Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation hace1_human E3 ubiquitin-protein ligase HACE1 KIAA1320 HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 HACE1 HECT-type E3 ubiquitin transferase HACE1 protein protein human Homo sapiens Human MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA optn_human Optineurin HYPL Optic neuropathy-inducing protein E3-14.7K-interacting protein FIP-2 Huntingtin-interacting protein L Huntingtin yeast partner L NEMO-related protein Transcription factor IIIA-interacting protein OPTN FIP2 GLC1E NRP HIP7 Huntingtin-interacting protein 7 protein protein human Homo sapiens Human MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL mlp3b_human Microtubule-associated proteins 1A/1B light chain 3B MAP1LC3B MAP1ALC3 Microtubule-associated protein 1 light chain 3 beta MAP1A/MAP1B light chain 3 B MAP1 light chain 3-like protein 2 Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B protein protein human Homo sapiens Human MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV ub2l3_human Ubiquitin-conjugating enzyme E2 L3 Ubiquitin-protein ligase L3 Ubiquitin carrier protein L3 UbcH7 Ubiquitin-conjugating enzyme E2-F1 L-UBC UBE2L3 UBCE7 UBCH7 E2 ubiquitin-conjugating enzyme L3 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD uba1_human Ubiquitin-like modifier-activating enzyme 1 Ubiquitin-activating enzyme E1 Protein A1S9 UBA1 A1S9T UBE1 protein protein human Homo sapiens Human MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ubp2_human Ubiquitin carboxyl-terminal hydrolase 2 Ubiquitin-specific-processing protease 2 Deubiquitinating enzyme 2 41 kDa ubiquitin-specific protease USP2 UBP41 Ubiquitin thioesterase 2 protein protein human Homo sapiens Human MSQLSSTLKRYTESARYTDAHYAKSGYGAYTPSSYGANLAASLLEKEKLGFKPVPTSSFLTRPRTYGPSSLLDYDRGRPLLRPDITGGGKRAESQTRGTERPLGSGLSGGSGFPYGVTNNCLSYLPINAYDQGVTLTQKLDSQSDLARDFSSLRTSDSYRIDPRNLGRSPMLARTRKELCTLQGLYQTASCPEYLVDYLENYGRKGSASQVPSQAPPSRVPEIISPTYRPIGRYTLWETGKGQAPGPSRSSSPGRDGMNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM optn_mouse Optineurin Optn protein protein mouse Mus musculus Mouse MSHQPLSCLTEKGDSPCETPGNGPSNMVHPSLDTFTPEELLQQMKELLVENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERLLFEMQSKEVKERLKALTHENERLKEELGKFKEKSEKPLEDLTGGYRYPRALEEEVEKLKTQVEQEVEHLKIQVMRLRAEKADLLGIVSELQLKLNSGGSSEDSFVEIRMTEGETEGAMKEMKNCPTPTRTDPISLSNCTEDARSCAEFEELTVSQLLLCLREGNQKVERLEVALREAKERISDFEKKANGHSSTEKQTARRADREKEDKGQESVGSEVETLSIQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSATPSELNEKQELVYSNKKLELQVESMRSEIKMEQAKTEEEKSRLATLQATHNKLLQEHNKALKTIEELTKQQAEKVDKMLLQELSEKLELAEQALASKQLQMDEMKQTLAKQEEDLETMAVLRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAILLKENNDIEEGGSRQSLMEMQCRHGARTSDSDQQTYLFQRGAEDRSWQHGQQPRSIPIHSCPKCGEVLPDIDTLQIHVMDCII hace1_mouse E3 ubiquitin-protein ligase HACE1 Hace1 Kiaa1320 HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 HECT-type E3 ubiquitin transferase HACE1 protein protein mouse Mus musculus Mouse MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSTDITSILLKQKGQDQEAPSLSAFEPPGPGSYESLPPGPGDSKPEVLAGEQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA sqstm_mouse Sequestosome-1 Ubiquitin-binding protein p62 STONE14 Sqstm1 A170 STAP protein protein mouse Mus musculus Mouse MASFTVKAYLLGKEEATREIRRFSFCFSPEPEAEAQAAAGPGPCERLLSRVAVLFPTLRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPPEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTTPESSSTGTEDKSNTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEEQMESGNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG optn-hace1-4 1562912 n/a 1562926 unspecified role unspecified role prey prey n/a 1562927 unspecified role unspecified role bait bait region ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1562928 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5Ci optn-hace1-6 1562912 n/a 1562927 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1562926 unspecified role unspecified role prey prey physical association physical association false false false 5Ciii optn-hace1-7 1562912 n/a 1562927 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562928 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 5 E/F p62 interacted with Lys48-linked poly-Ub chains but the Lys27-linked poly-Ub chain did not interact. optn-ubiq_human-6 1562913 n/a 1562926 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ank_1-6_region sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) n/a 1562927 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1G/H hace1-optn-13 1562913 n/a 1562927 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false huntingtin_interacting_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1562926 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1I map1lc3b-optn 1562913 n/a 1562927 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562929 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp 2H Lys193 to Arg substitution in human OPTN had no effect on this interaction. optn-hace1-1 1562914 n/a 1562926 unspecified role unspecified role prey prey n/a 1562927 unspecified role unspecified role bait bait physical association physical association false false false 1 C hace1-optn 1562914 n/a 1562926 unspecified role unspecified role bait bait n/a 1562927 unspecified role unspecified role prey prey physical association physical association false false false 1 D optn-hace1-2 1562915 n/a 1562927 unspecified role unspecified role bait bait n/a 1562926 unspecified role unspecified role prey prey physical association physical association false false false 1F optn-sqstm1-1 1562915 n/a 1562926 unspecified role unspecified role ancillary ancillary n/a 1562927 unspecified role unspecified role bait bait n/a 1562928 unspecified role unspecified role prey prey association association false false false 4D catalytic core of human ubiquitin-specific protease 2 (USP2) hace1-optn-6 1562916 n/a 1562930 unspecified role unspecified role ancillary ancillary n/a 1562931 unspecified role unspecified role ancillary ancillary n/a 1562932 unspecified role unspecified role ancillary ancillary n/a 1562927 enzyme target enzyme target substrate substrate unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562926 enzyme enzyme unspecified role unspecified role n/a 1562933 inhibitor inhibitor unspecified role unspecified role catalytic_core sufficient to bind sufficient binding region sufficient to bind range range range range false ubiquitination ubiquitination reaction ubiquitination false false false 2A Q96CV9 ubiquitinylated lysine hace1-optn-6 1562916 n/a 1562930 unspecified role unspecified role ancillary ancillary n/a 1562927 enzyme target enzyme target substrate substrate unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562932 unspecified role unspecified role ancillary ancillary n/a 1562931 unspecified role unspecified role ancillary ancillary n/a 1562926 enzyme enzyme unspecified role unspecified role ubiquitination ubiquitination reaction ubiquitination false false false 2A Q96CV9 ubiquitinylated lysine Q8IYU2 increases degradation at protein level of Q96CV9 optn-hace1-5 1562917 n/a 1562928 unspecified role unspecified role prey prey uba_domain required to bind necessary binding region required to bind range range range range false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562927 unspecified role unspecified role bait bait region ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 5Cii optn-sqstm1-6 1562917 n/a 1562927 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q96CV9:p.Lys193Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false n/a 1562928 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 5D optn-hace1 1562918 n/a 1562927 unspecified role unspecified role bait bait n/a 1562926 unspecified role unspecified role prey prey physical association physical association false false false no figure hace1-optn-11 1562919 n/a 1562927 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562926 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1E hace1-optn-7 1562920 n/a 1562926 enzyme enzyme unspecified role unspecified role n/a 1562930 unspecified role unspecified role ancillary ancillary n/a 1562931 unspecified role unspecified role ancillary ancillary n/a 1562932 unspecified role unspecified role ancillary ancillary n/a 1562927 enzyme target enzyme target substrate substrate unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ubiquitination ubiquitination reaction ubiquitination false false false 2E Supp 2 C/D/G Q96CV9 lys-55 N6-glycyllysine Q96CV9 lys-66 N6-glycyllysine Q96CV9 lys-78 N6-glycyllysine Q96CV9 lys-91 N6-glycyllysine Q96CV9 lys-193 N6-glycyllysine Q96CV9 lys-255 N6-glycyllysine Q96CV9 lys-319 N6-glycyllysine Q96CV9 lys-340 N6-glycyllysine Q96CV9 lys-351 N6-glycyllysine Q96CV9 lys-385 N6-glycyllysine Q96CV9 lys-429 N6-glycyllysine Q96CV9 lys-435 N6-glycyllysine Q8IYU2 increases degradation at protein level of Q96CV9 optn-hace1-3 1562921 n/a 1562934 unspecified role unspecified role bait bait n/a 1562935 unspecified role unspecified role prey prey n/a 1562936 unspecified role unspecified role prey prey association association false false false 4C optn-sqstm1-3 1562922 n/a 1562927 unspecified role unspecified role bait bait Q96CV9:p.Lys193Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562928 unspecified role unspecified role prey prey physical association physical association false false false 5Aii optn-sqstm1-5 1562923 n/a 1562927 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q96CV9:p.Lys193Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1562928 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false uba_domain required to bind necessary binding region required to bind range range range range false n/a 1562937 unspecified role unspecified role prey prey association association false false false 5Bi hace1-optn-8 1562924 n/a 1562926 unspecified role unspecified role neutral component neutral component n/a 1562927 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 1C Colocaliztion was seen mostly in extranuclear region of the cell. hace1-optn-12 1562925 n/a 1562926 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified possible_tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1562927 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 1J