IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rafidi-2013-2 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation rafidi-2013-1 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation rafidi-2013-5 crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation rafidi-2013-3 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation rafidi-2013-4 human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation rafidi-2013-6 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rafidi H., Mercado F., Astudillo M., Fry WH., Saldana M., Carraway KL., Sweeney C. Cancer - Interactions investigated in the context of cancer Transfected Erbb2 and EGFR have been assumed to be of Human origin as previous work by authors uses the human proteins. (references 1,3,6 et al.) casweeney@ucdavis.edu. The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation lrig3_human Leucine-rich repeats and immunoglobulin-like domains protein 3 LRIG3 LIG3 UNQ287/PRO326/PRO335 protein protein human Homo sapiens Human MSAPSLRARAAGLGLLLCAVLGRAGRSDSGGRGELGQPSGVAAERPCPTTCRCLGDLLDCSRKRLARLPEPLPSWVARLDLSHNRLSFIKASSMSHLQSLREVKLNNNELETIPNLGPVSANITLLSLAGNRIVEILPEHLKEFQSLETLDLSSNNISELQTAFPALQLKYLYLNSNRVTSMEPGYFDNLANTLLVLKLNRNRISAIPPKMFKLPQLQHLELNRNKIKNVDGLTFQGLGALKSLKMQRNGVTKLMDGAFWGLSNMEILQLDHNNLTEITKGWLYGLLMLQELHLSQNAINRISPDAWEFCQKLSELDLTFNHLSRLDDSSFLGLSLLNTLHIGNNRVSYIADCAFRGLSSLKTLDLKNNEISWTIEDMNGAFSGLDKLRRLILQGNRIRSITKKAFTGLDALEHLDLSDNAIMSLQGNAFSQMKKLQQLHLNTSSLLCDCQLKWLPQWVAENNFQSFVNASCAHPQLLKGRSIFAVSPDGFVCDDFPKPQITVQPETQSAIKGSNLSFICSAASSSDSPMTFAWKKDNELLHDAEMENYAHLRAQGGEVMEYTTILRLREVEFASEGKYQCVISNHFGSSYSVKAKLTVNMLPSFTKTPMDLTIRAGAMARLECAAVGHPAPQIAWQKDGGTDFPAARERRMHVMPEDDVFFIVDVKIEDIGVYSCTAQNSAGSISANATLTVLETPSFLRPLLDRTVTKGETAVLQCIAGGSPPPKLNWTKDDSPLVVTERHFFAAGNQLLIIVDSDVSDAGKYTCEMSNTLGTERGNVRLSVIPTPTCDSPQMTAPSLDDDGWATVGVVIIAVVCCVVGTSLVWVVIIYHTRRRNEDCSITNTDETNLPADIPSYLSSQGTLADRQDGYVSSESGSHHQFVTSSGAGFFLPQHDSSGTCHIDNSSEADVEAATDLFLCPFLGSTGPMYLKGNVYGSDPFETYHTGCSPDPRTVLMDHYEPSYIKKKECYPCSHPSEESCERSFSNISWPSHVRKLLNTSYSHNEGPGMKNLCLNKSSLDFSANPEPASVASSNSFMGTFGKALRRPHLDAYSSFGQPSDCQPRAFYLKAHSSPDLDSGSEEDGKERTDFQEENHICTFKQTLENYRTPNFQSYDLDT lrig1_human Leucine-rich repeats and immunoglobulin-like domains protein 1 LRIG1 LIG1 protein protein human Homo sapiens Human MARPVRGGLGAPRRSPCLLLLWLLLLRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATGHPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKS erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV lrig3-lrig1-2 3663807 N/A 3663813 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd_region required to bind necessary binding region required to bind range range range range false N/A 3663814 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 11C lrig1-lrig3-1 3663807 N/A 3663815 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3663813 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3663814 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false association association false false false Figure 4 erbb2-lrig1-1 3663807 N/A 3663815 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3663814 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 4 Lrig3 (Q6UXM1) The amount of Lrig1 precipitating with ErbB2 reproducibly decreased when Lrig3 was co-expressed. lrig3-lrig1-1 3663808 N/A 3663813 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3663814 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd_region required to bind necessary binding region required to bind range range range range false myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 9C lrig3-lrig1-7 3663808 N/A 3663815 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3663814 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false ecd_region required to bind necessary binding region required to bind range range range range false physical association physical association false false false Figure 10C lrig3-lrig1-5 3663809 N/A 3663813 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3663814 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6D lrig3-lrig1-6 3663809 N/A 3663813 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3663814 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6D lrig3-lrig1-3 3663810 N/A 3663813 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3663814 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 6B lrig3-lrig1-4 3663811 N/A 3663813 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3663814 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 6C lrig1-lrig3-2 3663812 N/A 3663813 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3663814 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc_tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6E