IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ holderfield-2013-3 RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. human-sw620 Human caucasian colon adenocarcinoma SW620 cells sw620 Human caucasian colon adenocarcinoma SW620 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation holderfield-2013-1 RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. human-hey8 Homo sapiens moderately differentiated papillary ovarian cystoadenocarcinoma cell line. hey8 HEY 8 Human moderately differentiated papillary ovarian cystoadenocarcinoma cell line. Established from a peritoneal deposit of a moderately differentiated papillary ovarian cystoadenocarcinoma and has been reported to be moderately resistant to chemotherapy anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation holderfield-2013-2 RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH raf1-braf-13 1420793 n/a 1420796 unspecified role unspecified role bait bait n/a 1420797 unspecified role unspecified role prey prey physical association physical association false false false 6A Compound A -a RAF inhibitor. braf-raf1-25 1420793 n/a 1420797 unspecified role unspecified role bait bait n/a 1420796 unspecified role unspecified role prey prey physical association physical association false false false 6Ai Compound A -a RAF inhibitor. raf1-braf-11 1420794 n/a 1420796 unspecified role unspecified role bait bait n/a 1420797 unspecified role unspecified role prey prey P15056:p.Gly464Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5C Compound A -a RAF inhibitor. raf1-braf-12 1420795 n/a 1420797 unspecified role unspecified role prey prey P15056:p.Gly464Val mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1420796 unspecified role unspecified role bait bait physical association physical association false false false 5C Compound A -a RAF inhibitor.