IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lu-2013a-5 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-1 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. human-wm115 Human WM115 Melanoma cell line wm115 WM115 Human melanoma cell line http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=CRL-1675&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-2 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. human-sk_mel_23 Homo sapiens sk_mel_23 Human SK_MEL_23 - Melanoma cell line with WT p53 I cannot find a primary reference - as far back as 1991 they refer to it - but I dont think it would be possible to find any pmid reference before this... Houghton, A.N. et al. J. Exp. Med. 164 , 812-829 (1987). unpublished subline. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-3 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. human-wm278 WM278 Non-metastatic Human Melanoma cell line with mutation BRAF V600E wm278 WM278 Non-metastatic Melanoma cell line with mutation BRAF V600E Coriell Institute Catalog ID: WC00054 Wistar Collection anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-4 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. human-igr-37 Human IGR-37 Melanoma cell line from a lymph node metastasis igr-37 Human IGR-37 Melanoma cell line from a lymph node metastasis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-6 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-7 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-8 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-9 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. mouse-b16 Mus musculus melanoma tumor maintained in mouse. b16 mouse skin melanoma tumor maintained in C57/B1 mouse fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation lu-2013a-10 Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP. human-a375p A375P Human Melanoma cell line a375p A375P Human Melanoma cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Lu M., Breyssens H., Salter V., Zhong S., Hu Y., Baer C., Ratnayaka I., Sullivan A., Brown N.R., Endicott J., Knapp S., Kessler B.M., Middleton M.R., Siebold C., Jones E.Y., Sviderskaya E.V., Cebon J., John T., Caballero O.L., Goding C.R., Lu X. Cancer - Interactions investigated in the context of cancer Interactions involving "Histone H1" have not been entered as the origin and identity of the molecule are not known. iASPP constructs have been assumed to be Human in origin based on molecule length and mutation mapping. The CyclinB1/CDK1 complex was found to be of Spiny Starfish (Marthasterias glacialis) origin based on precious work by J.Endicott - a co-author and supplier of this complex. Plasmids expressing cyclin B1 and CDK1 that were a gift from Jonathon Pines (as probably used in Figure 7B) were assumed to be Human in origin, based on previous work. xin.lu@ludwig.ox.ac.uk Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation ccnb_margl G2/mitotic-specific cyclin-B protein protein margl Marthasterias glacialis MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGMTKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFDNPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQETLFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQIRSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPSEIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKKYSSAKFMNVSTISALTSSTVMDLADQMC cdk1_margl protein protein margl Marthasterias glacialis iaspp_human RelA-associated inhibitor PPP1R13L IASPP NKIP1 PPP1R13BL RAI Inhibitor of ASPP protein NFkB-interacting protein 1 PPP1R13B-like protein protein protein human Homo sapiens Human MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQPQPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD iaspp_mouse RelA-associated inhibitor Ppp1r13l Nkip1 Inhibitor of ASPP protein NFkB-interacting protein 1 PPP1R13B-like protein protein protein mouse Mus musculus Mouse MDSEAFQHARDLLDLNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGAQAGVPSRMARYSTSPVPEHFGSRGSPQKIATDGIEARFGRSESAPSLHPYSPLSPKGRPSSPRTPIYLQPDTYSSLDRAPSPRPRAFDGAGSPHGRAPSPRPGIGPVRQPGPSTPFDYLGRAGSPRGSPLAEGPQAFFPERGPSPRPPAAAYDTAGTFGSPLLGAGGSAFTPPLRAQDDSTLRRRPPKAWNESDLDVAYEKKSSQTASYERLDVFTRPASPGLQLLPWRESSLDGLGASGKDHLTSATLPRNYKVSPLASDRRSDVGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRSTPRQRPIPLSMIFKLQNAFWEHGAGRAVLPGSPIFSRAPPPKLPPQPPPQPQMQPQPQPQPQMQPQSQAQPQTPAPQQTWSPMNEGLLKSPAELEPEPELEVLLAPVEEAGDADEGTVTRPLSPTRLQPALPPEAQTVPELEEVARVLAEIPRPLKRRGSMEQSPAVALPPTHKKQYQQIINRLFHRHGGPGPGGPEPELSTITEGSEARAGPPAPAPPAPIPPPAPPQSSPPEQPQSMEMRSVLRKVGSPRKARRARLNPLVLLLDAALTGELDVVQQAVKEMNDPSQPNEEGITALHNAICGANYPIVDFLIAAGANVNSPDSHGWTPLHCAASCNDTAICTALVQHGAAIFATTLSDGATAIEKCDPYREGYADCATYLADVEQSMGLMHNGVVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWASLHGQEGYVPRNYFGLFPRVKSQRSKI ppp1r13l-cdk1-4 1390798 n/a 1390808 unspecified role unspecified role ancillary ancillary n/a 1390809 unspecified role unspecified role prey prey n/a 1390810 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 3B ppp1r13l-cdk1-1 1390798 n/a 1390810 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1390810 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Ser84Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Ser113Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Ser113Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.[Ser84Asp;Ser113Asp] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Ser84Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4A/B ppp1r13l-cdk1-2 1390798 n/a 1390810 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1390810 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. physical association physical association false false false 4C ppp1r13l-cdk1-3 1390798 n/a 1390810 unspecified role unspecified role prey prey human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. n/a 1390810 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 4D ppp1r13l-ppp1r13l 1390798 n/a 1390810 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1390810 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Thr722Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8WUF5:p.Tyr814Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.[Leu724Ala;Tyr814Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Leu724Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.[Thr722Ala;Asn813Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Asn813Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.[Thr722Ala;Tyr814Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.[Leu724Ala;Asn813Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 1390850 1390847 direct interaction direct interaction false false false 4F, Supp 4B. ppp1r13l-ppp1r13l-1 1390798 n/a 1390810 unspecified role unspecified role prey prey Q8WUF5:p.[Ser84Asp;Ser113Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1390811 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 5A tp53-ppp1r13l 1390799 n/a 1390810 unspecified role unspecified role prey prey n/a 1390811 unspecified role unspecified role bait bait physical association physical association false false false 1E p53 selectively coimmunoprecipitated slow-migrating (nuclear) iASPP in the panel of melanoma cell lines tested. tp53-ppp1r13l-1 1390800 n/a 1390810 unspecified role unspecified role prey prey n/a 1390811 unspecified role unspecified role bait bait physical association physical association false false false 1E p53 selectively coimmunoprecipitated slow-migrating (nuclear) iASPP in the panel of melanoma cell lines tested. tp53-ppp1r13l-2 1390801 n/a 1390811 unspecified role unspecified role bait bait n/a 1390810 unspecified role unspecified role prey prey physical association physical association false false false 1E, 7D. p53 selectively coimmunoprecipitated slow-migrating iASPP in the panel of melanoma cell lines tested. (CIP) -calf intestine alkaline phosphatase. JNJ-7706621 (JNJ), a CDK1 inhibitor. Nocodazole (an anti-neoplastic agent interfering with microtubule polymerization.) It increases amounts of the slow-migrating form of iASPP. tp53-ppp1r13l-3 1390802 n/a 1390810 unspecified role unspecified role prey prey n/a 1390811 unspecified role unspecified role bait bait physical association physical association false false false 1E p53 selectively coimmunoprecipitated slow-migrating (nuclear) iASPP in the panel of melanoma cell lines tested. ccnb1-cdk1 1390803 n/a 1390808 ancillary ancillary unspecified role unspecified role n/a 1390810 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.[Ser84Ala;Ser113Ala] mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Ser84Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8WUF5:p.Ser113Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1390809 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation false false false 3C/D tp53-ppp1r13l-4 1390804 n/a 1390811 unspecified role unspecified role bait bait purified purified n/a 1390810 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8WUF5:p.[Ser84Asp;Ser113Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5B cdk1-ppp1r13l-1 1390805 n/a 1390810 unspecified role unspecified role neutral component neutral component n/a 1390812 unspecified role unspecified role neutral component neutral component purified purified colocalization colocalization false false false 7B ppp1r13l-tp53-3 1390806 n/a 1390813 unspecified role unspecified role neutral component neutral component n/a 1390814 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 7K JNJ-7706621 (JNJ), a CDK1 inhibitor. cdk1-ppp1r13l-2 1390807 n/a 1390811 unspecified role unspecified role neutral component neutral component purified purified n/a 1390810 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 8I JNJ-7706621 (JNJ), a CDK1 inhibitor.