IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hu-2012-10 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-12 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer Re-ChIP experiment:- sequential immunoprecipitations where the bait of the first IP is prey for the second. This method allows for the identification of multiple (concurrently binding) proteins on a single DNA sequence. These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-3 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-11 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-4 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-5 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line kyse-410 KYSE-410 -Human esophageal squamous cell carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-2 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-7 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-8 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-1 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer The peptide identifications for "DDB1" are erroneous: all peptide sequences given in Table S1 correspond to the protein PDIA3_Human -(UniProt P30101), which has few regions of homology with DDB1. The authors have however confirmed that the identification of DDB1 was correct. These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation hu-2012-6 CRL4B Catalyzes H2AK119 Monoubiquitination and Coordinates with PRC2 to Promote Tumorigenesis. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Hu H., Yang Y., Ji Q., Zhao W., Jiang B., Liu R., Yuan J., Liu Q., Li X., Zou Y., Shao C., Shang Y., Wang Y., Gong Y. Cancer - Interactions investigated in the context of cancer HeLa cell nuclear extracts were fractionated on a DEAE sepharose column followed by gel filtration. These constructs/proteins have been confirmed by the authors to be of Human origin:- FLAG-CUL4B and deletion mutants, FLAG-RING1B, GST-DDB1,in-vitro-transcribed/translated _RbAp46 and RbAp48, Sf9 produced - purified _His6-H2A_ and mutations. yanwang@tmu.edu.cn, yxg8@sdu.edu.cn Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation pten_human_gene PTEN_HUMAN_GENE gene gene human Homo sapiens Human cul4b_human Cullin-4B CUL4B KIAA0695 protein protein human Homo sapiens Human MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA socs1_human_gene SOCS1_HUMAN_GENE gene gene human Homo sapiens Human axin2_human_gene gene gene human Homo sapiens Human foxo3_human_gene FOXO3_HUMAN_GENE gene gene human Homo sapiens Human siah1_human_gene SIAH1_HUMAN_GENE gene gene human Homo sapiens Human e2f1_human_gene Human transcription factor E2F1 gene gene human Homo sapiens Human runx1_human_gene Human RUNX1 gene : Runt-related transcription factor 1 gene gene human Homo sapiens Human fancb_human_gene FANCB_HUMAN_GENE gene gene human Homo sapiens Human mcm8_human_gene MCM8_HUMAN_GENE gene gene human Homo sapiens Human nkd2_human_gene NKD2_HUMAN_GENE gene gene human Homo sapiens Human co4a1_human_gene CO4A1_HUMAN_GENE gene gene human Homo sapiens Human ibp3_human_gene IBP3_HUMAN_GENE gene gene human Homo sapiens Human mll3_human_gene MLL3_HUMAN_GENE gene gene human Homo sapiens Human cabl2_human_gene CABL2_HUMAN_GENE gene gene human Homo sapiens Human rnh1_human_gene RNH1_HUMAN_GENE gene gene human Homo sapiens Human tgfb1_human_gene TGFB1_HUMAN_GENE gene gene human Homo sapiens Human ezh2_human Histone-lysine N-methyltransferase EZH2 KMT6 Enhancer of zeste homolog 2 ENX-1 Lysine N-methyltransferase 6 EZH2 protein protein human Homo sapiens Human MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP cd2a2_human_gene Cyclin-dependent kinase inhibitor 2A- CDKN2A gene gene human Homo sapiens Human suz12_human Polycomb protein SUZ12 CHET9 JJAZ1 KIAA0160 SUZ12 Suppressor of zeste 12 protein homolog Joined to JAZF1 protein Chromatin precipitated E2F target 9 protein protein protein human Homo sapiens Human MAPQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL ddb1_human DNA damage-binding protein 1 DDB1 Damage-specific DNA-binding protein 1 UV-damaged DNA-binding factor DDB p127 subunit DNA damage-binding protein a UV-damaged DNA-binding protein 1 Xeroderma pigmentosum group E-complementing protein XPE-binding factor HBV X-associated protein 1 XAP1 protein protein human Homo sapiens Human MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPNRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQAKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH eed_human Polycomb protein EED EED WD protein associating with integrin cytoplasmic tails 1 Embryonic ectoderm development protein protein protein human Homo sapiens Human MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR bmi1_human Polycomb complex protein BMI-1 BMI1 PCGF4 RNF51 Polycomb group RING finger protein 4 RING finger protein 51 protein protein human Homo sapiens Human MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG ring2_human E3 ubiquitin-protein ligase RING2 RING finger protein 2 RING finger protein 1B RING finger protein BAP-1 Protein DinG Huntingtin-interacting protein 2-interacting protein 3 RNF2 BAP1 DING HIPI3 RING1B RING-type E3 ubiquitin transferase RING2 protein protein human Homo sapiens Human MSQAVQTNGTQPLSKTWELSLYELQRTPQEAITDGLEIVVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAMAIDPVMDGASEIELVFRPHPTLMEKDDSAQTRYIKTSGNATVDHLSKYLAVRLALEELRSKGESNQMNLDTASEKQYTIYIATASGQFTVLNGSFSLELVSEKYWKVNKPMELYYAPTKEHK rbx1_human E3 ubiquitin-protein ligase RBX1 RBX1 RNF75 ROC1 Regulator of cullins 1 RING finger protein 75 Protein ZYP RING-box protein 1 E3 ubiquitin-protein transferase RBX1 protein protein human Homo sapiens Human MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH jard2_human Protein Jumonji JARID2 JMJ Jumonji/ARID domain-containing protein 2 protein protein human Homo sapiens Human MSKERPKRNIIQKKYDDSDGIPWSEERVVRKVLYLSLKEFKNSQKRQHAEGIAGSLKTVNGLLGNDQSKGLGPASEQSENEKDDASQVSSTSNDVSSSDFEEGPSRKRPRLQAQRKFAQSQPNSPSTTPVKIVEPLLPPPATQISDLSKRKPKTEDFLTFLCLRGSPALPNSMVYFGSSQDEEEVEEEDDETEDVKTATNNASSSCQSTPRKGKTHKHVHNGHVFNGSSRSTREKEPVQKHKSKEATPAKEKHSDHRADSRREQASANHPAAAPSTGSSAKGLAATHHHPPLHRSAQDLRKQVSKVNGVTRMSSLGAGVTSAKKMREVRPSPSKTVKYTATVTKGAVTYTKAKRELVKDTKPNHHKPSSAVNHTISGKTESSNAKTRKQVLSLGGASKSTGPAVNGLKVSGRLNPKSCTKEVGGRQLREGLQLREGLRNSKRRLEEAHQAEKPQSPPKKMKGAAGPAEGPGKKAPAERGLLNGHVKKEVPERSLERNRPKRATAGKSTPGRQAHGKADSASCENRSTSQPESVHKPQDSGKAEKGGGKAGWAAMDEIPVLRPSAKEFHDPLIYIESVRAQVEKFGMCRVIPPPDWRPECKLNDEMRFVTQIQHIHKLGRRWGPNVQRLACIKKHLKSQGITMDELPLIGGCELDLACFFRLINEMGGMQQVTDLKKWNKLADMLRIPRTAQDRLAKLQEAYCQYLLSYDSLSPEEHRRLEKEVLMEKEILEKRKGPLEGHTENDHHKFHPLPRFEPKNGLIHGVAPRNGFRSKLKEVGQAQLKTGRRRLFAQEKEVVKEEEEDKGVLNDFHKCIYKGRSVSLTTFYRTARNIMSMCFSKEPAPAEIEQEYWRLVEEKDCHVAVHCGKVDTNTHGSGFPVGKSEPFSRHGWNLTVLPNNTGSILRHLGAVPGVTIPWLNIGMVFSTSCWSRDQNHLPYIDYLHTGADCIWYCIPAEEENKLEDVVHTLLQANGTPGLQMLESNVMISPEVLCKEGIKVHRTVQQSGQFVVCFPGSFVSKVCCGYSVSETVHFATTQWTSMGFETAKEMKRRHIAKPFSMEKLLYQIAQAEAKKENGPTLSTISALLDELRDTELRQRRQLFEAGLHSSARYGSHDGSSTVADGKKKPRKWLQLETSERRCQICQHLCYLSMVVQENENVVFCLECALRHVEKQKSCRGLKLMYRYDEEQIISLVNQICGKVSGKNGSIENCLSKPTPKRGPRKRATVDVPPSRLSASSSSKSASSSS phf1_human PHD finger protein 1 PCL1 Polycomb-like protein 1 PHF1 protein protein human Homo sapiens Human MAQPPRLSRSGASSLWDPASPAPTSGPRPRLWEGQDVLARWTDGLLYLGTIKKVDSAREVCLVQFEDDSQFLVLWKDISPAALPGEELLCCVCRSETVVPGNRLVSCEKCRHAYHQDCHVPRAPAPGEGEGTSWVCRQCVFAIATKRGGALKKGPYARAMLGMKLSLPYGLKGLDWDAGHLSNRQQSYCYCGGPGEWNLKMLQCRSCLQWFHEACTQCLSKPLLYGDRFYEFECCVCRGGPEKVRRLQLRWVDVAHLVLYHLSVCCKKKYFDFDREILPFTSENWDSLLLGELSDTPKGERSSRLLSALNSHKDRFISGREIKKRKCLFGLHARMPPPVEPPTGDGALTSFPSGQGPGGGVSRPLGKRRRPEPEPLRRRQKGKVEELGPPSAVRNQPEPQEQRERAHLQRALQASVSPPSPSPNQSYQGSSGYNFRPTDARCLPSSPIRMFASFHPSASTAGTSGDSGPPDRSPLELHIGFPTDIPKSAPHSMTASSSSVSSPSPGLPRRSAPPSPLCRSLSPGTGGGVRGGVGYLSRGDPVRVLARRVRPDGSVQYLVEWGGGGIF rbbp4_human Histone-binding protein RBBP4 Retinoblastoma-binding protein 4 Retinoblastoma-binding protein p48 Chromatin assembly factor 1 subunit C Chromatin assembly factor I p48 subunit Nucleosome-remodeling factor subunit RBAP48 RBBP4 RBAP48 protein protein human Homo sapiens Human MADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDPEGQGS rbbp7_human Histone-binding protein RBBP7 RBBP7 RBAP46 Retinoblastoma-binding protein 7 Retinoblastoma-binding protein p46 Histone acetyltransferase type B subunit 2 Nucleosome-remodeling factor subunit RBAP46 protein protein human Homo sapiens Human MASKEMFEDTVEERVINEEYKIWKKNTPFLYDLVMTHALQWPSLTVQWLPEVTKPEGKDYALHWLVLGTHTSDEQNHLVVARVHIPNDDAQFDASHCDSDKGEFGGFGSVTGKIECEIKINHEGEVNRARYMPQNPHIIATKTPSSDVLVFDYTKHPAKPDPSGECNPDLRLRGHQKEGYGLSWNSNLSGHLLSASDDHTVCLWDINAGPKEGKIVDAKAIFTGHSAVVEDVAWHLLHESLFGSVADDQKLMIWDTRSNTTSKPSHLVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHTFESHKDEIFQVHWSPHNETILASSGTDRRLNVWDLSKIGEEQSAEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQIWQMAENIYNDEESDVTTSELEGQGS gal4_yeast_gene YEAST GAL4 Gene gene gene yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fancm_human Fanconi anemia group M protein FANCM KIAA1596 ATP-dependent RNA helicase FANCM Fanconi anemia-associated polypeptide of 250 kDa Protein Hef ortholog protein protein human Homo sapiens Human MSGRQRTLFQTWGSSISRSSGTPGCSSGTERPQSPGSSKAPLPAAAEAQLESDDDVLLVAAYEAERQLCLENGGFCTSAGALWIYPTNCPVRDYQLHISRAALFCNTLVCLPTGLGKTFIAAVVMYNFYRWFPSGKVVFMAPTKPLVTQQIEACYQVMGIPQSHMAEMTGSTQASTRKEIWCSKRVLFLTPQVMVNDLSRGACPAAEIKCLVIDEAHKALGNYAYCQVVRELVKYTNHFRILALSATPGSDIKAVQQVITNLLIGQIELRSEDSPDILTYSHERKVEKLIVPLGEELAAIQKTYIQILESFARSLIQRNVLMRRDIPNLTKYQIILARDQFRKNPSPNIVGIQQGIIEGEFAICISLYHGYELLQQMGMRSLYFFLCGIMDGTKGMTRSKNELGRNEDFMKLYNHLECMFARTRSTSANGISAIQQGDKNKKFVYSHPKLKKLEEVVIEHFKSWNAENTTEKKRDETRVMIFSSFRDSVQEIAEMLSQHQPIIRVMTFVGHASGKSTKGFTQKEQLEVVKQFRDGGYNTLVSTCVGEEGLDIGEVDLIICFDSQKSPIRLVQRMGRTGRKRQGRIVIILSEGREERIYNQSQSNKRSIYKAISSNRQVLHFYQRSPRMVPDGINPKLHKMFITHGVYEPEKPSRNLQRKSSIFSYRDGMRQSSLKKDWFLSEEEFKLWNRLYRLRDSDEIKEITLPQVQFSSLQNEENKPAQESTTGIHQLSLSEWRLWQDHPLPTHQVDHSDRCRHFIGLMQMIEGMRHEEGECSYELEVESYLQMEDVTSTFIAPRNESNNLASDTFITHKKSSFIKNINQGSSSSVIESDEECAEIVKQTHIKPTKIVSLKKKVSKEIKKDQLKKENNHGIIDSVDNDRNSTVENIFQEDLPNDKRTSDTDEIAATCTINENVIKEPCVLLTECQFTNKSTSSLAGNVLDSGYNSFNDEKSVSSNLFLPFEEELYIVRTDDQFYNCHSLTKEVLANVERFLSYSPPPLSGLSDLEYEIAKGTALENLLFLPCAEHLRSDKCTCLLSHSAVNSQQNLELNSLKCINYPSEKSCLYDIPNDNISDEPSLCDCDVHKHNQNENLVPNNRVQIHRSPAQNLVGENNHDVDNSDLPVLSTDQDESLLLFEDVNTEFDDVSLSPLNSKSESLPVSDKTAISETPLVSQFLISDELLLDNNSELQDQITRDANSFKSRDQRGVQEEKVKNHEDIFDCSRDLFSVTFDLGFCSPDSDDEILEHTSDSNRPLDDLYGRYLEIKEISDANYVSNQALIPRDHSKNFTSGTVIIPSNEDMQNPNYVHLPLSAAKNEELLSPGYSQFSLPVQKKVMSTPLSKSNTLNSFSKIRKEILKTPDSSKEKVNLQRFKEALNSTFDYSEFSLEKSKSSGPMYLHKSCHSVEDGQLLTSNESEDDEIFRRKVKRAKGNVLNSPEDQKNSEVDSPLHAVKKRRFPINRSELSSSDESENFPKPCSQLEDFKVCNGNARRGIKVPKRQSHLKHVARKFLDDEAELSEEDAEYVSSDENDESENEQDSSLLDFLNDETQLSQAINDSEMRAIYMKSLRSPMMNNKYKMIHKTHKNINIFSQIPEQDETYLEDSFCVDEEESCKGQSSEEEVCVDFNLITDDCFANSKKYKTRRAVMLKEMMEQNCAHSKKKLSRIILPDDSSEEENNVNDKRESNIAVNPSTVKKNKQQDHCLNSVPSGSSAQSKVRSTPRVNPLAKQSKQTSLNLKDTISEVSDFKPQNHNEVQSTTPPFTTVDSQKDCRKFPVPQKDGSALEDSSTSGASCSKSRPHLAGTHTSLRLPQEGKGTCILVGGHEITSGLEVISSLRAIHGLQVEVCPLNGCDYIVSNRMVVERRSQSEMLNSVNKNKFIEQIQHLQSMFERICVIVEKDREKTGDTSRMFRRTKSYDSLLTTLIGAGIRILFSSCQEETADLLKELSLVEQRKNVGIHVPTVVNSNKSEALQFYLSIPNISYITALNMCHQFSSVKRMANSSLQEISMYAQVTHQKAEEIYRYIHYVFDIQMLPNDLNQDRLKSDI hsp7c_human Heat shock cognate 71 kDa protein HSPA8 HSC70 HSP73 HSPA10 Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1 protein protein human Homo sapiens Human MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD grwd1_human Glutamate-rich WD repeat-containing protein 1 GRWD1 GRWD WDR28 KIAA1942 protein protein human Homo sapiens Human MAARKGRRRTCETGEPMEAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSEGSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIHLWTPTDGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACMLTTATAHDGDVNVISWSRREPFLLSGGDDGALKIWDLRQFKSGSPVATFKQHVAPVTSVEWHPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVHQGETELKELHWHPQCPGLLVSTALSGFTIFRTISV csn5_human COP9 signalosome complex subunit 5 Jun activation domain-binding protein 1 COPS5 CSN5 JAB1 protein protein human Homo sapiens Human MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS csn4_human COP9 signalosome complex subunit 4 COPS4 CSN4 JAB1-containing signalosome subunit 4 protein protein human Homo sapiens Human MAAAVRQDLAQLMNSSGSHKDLAGKYRQILEKAIQLSGAEQLEALKAFVEAMVNENVSLVISRQLLTDFCTHLPNLPDSTAKEIYHFTLEKIQPRVISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPLETGQKQYNVDYKLETYLKIARLYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYKVCYARVLDYRRKFIEAAQRYNELSYKTIVHESERLEALKHALHCTILASAGQQRSRMLATLFKDERCQQLAAYGILEKMYLDRIIRGNQLQEFAAMLMPHQKATTADGSSILDRAVIEHNLLSASKLYNNITFEELGALLEIPAAKAEKIASQMITEGRMNGFIDQIDGIVHFETREALPTWDKQIQSLCFQVNNLLEKISQTAPEWTAQAMEAQMAQ csn3_human COP9 signalosome complex subunit 3 COPS3 CSN3 JAB1-containing signalosome subunit 3 protein protein human Homo sapiens Human MASALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTSIHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPSSYS cul4b-pten 1350257 n/a 1350268 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 4B-1,4D/E,5D, 5F Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. cul4b-pten-1 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350270 unspecified role unspecified role prey prey association association false false false 4B-2 cul4b-socs1 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350271 unspecified role unspecified role prey prey association association false false false 4B-3 cul4b-axin2 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350271 unspecified role unspecified role prey prey association association false false false 4B-4 cul4b-axin2-1 1350257 n/a 1350272 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 4B-5 cul4b-foxo3 1350257 n/a 1350273 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 4B-6 cul4b-siah1 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350274 unspecified role unspecified role prey prey association association false false false 4D/E cul4b-e2f1 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350275 unspecified role unspecified role prey prey association association false false false 4B-8 cul4b-runx1 1350257 n/a 1350276 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 4B-9 cul4b-fancb 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350277 unspecified role unspecified role prey prey association association false false false 4B-10 cul4b-mcm8 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350278 unspecified role unspecified role prey prey association association false false false 4B-11 cul4b-nkd2 1350257 n/a 1350279 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 4B-12 cul4b-co4a1 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350280 unspecified role unspecified role prey prey association association false false false 4B-13 cul4b-cdkn2a 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350281 unspecified role unspecified role prey prey association association false false false 4B-15 cul4b-mll3 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350282 unspecified role unspecified role prey prey association association false false false 4B-16 cul4b-cabl2 1350257 n/a 1350283 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 4B-17 cul4b-rnh1 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350284 unspecified role unspecified role prey prey association association false false false 4B-18 cul4b-siah1-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350274 unspecified role unspecified role prey prey association association false false false 4B-7 cul4b-cdkn2a-1 1350257 n/a 1350281 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait association association false false false 4B-15 cul4b-mcm8-1 1350257 n/a 1350278 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait association association false false false 4B-11 cul4b-cabl2-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350283 unspecified role unspecified role prey prey association association false false false 4B-17 cul4b-fancb-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350277 unspecified role unspecified role prey prey association association false false false 4B-10 cul4b-foxo3-1 1350257 n/a 1350273 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait association association false false false 4B-6 cul4b-rnh1-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350284 unspecified role unspecified role prey prey association association false false false 4B-18 cul4b-runx1-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350276 unspecified role unspecified role prey prey association association false false false 4B-9 cul4b-nkd2-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350279 unspecified role unspecified role prey prey association association false false false 4B-12 cul4b-socs1-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350271 unspecified role unspecified role prey prey association association false false false 4B-3 cul4b-co4a1-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350280 unspecified role unspecified role prey prey association association false false false 4B-13 cul4b-e2f1-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350275 unspecified role unspecified role prey prey association association false false false 4B-8 cul4b-axin2-2 1350257 n/a 1350272 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait association association false false false 4B-5 cul4b-axin2-2 1350257 n/a 1350271 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait association association false false false 4B-4 cul4b-mll3-1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350282 unspecified role unspecified role prey prey association association false false false 4B-16 cul4b-pten-2 1350257 n/a 1350270 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait association association false false false 4B-2 cul4b-siah1-2 1350257 n/a 1350273 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-axin2 1350257 n/a 1350271 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-cdkn2a-2 1350257 n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 4B/D/E, 5D, 5F. Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. ezh2-ibp3 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350280 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-pten 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4B/D/E, 5D/F. Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. ezh2-mcm8 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350277 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-fancb-2 1350257 n/a 1350276 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-fancb 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350276 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-foxo3 1350257 n/a 1350272 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-e2f1 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350274 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-axin2-3 1350257 n/a 1350271 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-ibp3-2 1350257 n/a 1350280 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-co4a1 1350257 n/a 1350279 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-co4a1-2 1350257 n/a 1350279 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-foxo3-2 1350257 n/a 1350272 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-e2f1-2 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350274 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-siah1 1350257 n/a 1350273 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. cul4b-mcm8-2 1350257 n/a 1350269 unspecified role unspecified role bait bait n/a 1350277 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4D/E EZH2 depletion had little effect on the recruitment of CUL4B to target promoters;however, in CUL4B-depleted cells, the recruitment of EZH2 was greatly reduced. ezh2-cdkn2a 1350257 n/a 1350285 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4B/D/E, 5D, 5F Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. suz12-pten 1350257 n/a 1350287 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5D, 5F. Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. ddb1-pten 1350257 n/a 1350288 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5D, 5F. Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. suz12-cdkn2a 1350257 n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350287 unspecified role unspecified role bait bait association association false false false 5D, 5F Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. ddb1-cdkn2a-1 1350257 n/a 1350288 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5F. Knockdown of the expression of CUL4B or DDB1 led to a significant reduction of the binding of EZH2 and SUZ12 to the promoters of p16 and PTEN. Depletion of EZH2 or SUZ12 expression resulted in only a marginal decrease in the association of CUL4B and DDB1 with these promoters. ddb1-cul4b-2 1350258 n/a 1350269 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C ezh2-cdkn2a-3 1350258 n/a 1350269 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C suz12-cdkn2a-1 1350258 n/a 1350269 unspecified role unspecified role prey prey n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350287 unspecified role unspecified role bait bait association association false false false 5C cul4b-cdkn2a-6 1350258 n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait n/a 1350288 unspecified role unspecified role prey prey association association false false false 5C ezh2-cul4b 1350258 n/a 1350269 unspecified role unspecified role prey prey n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait association association false false false 5C ddb1-cul4b-3 1350258 n/a 1350285 unspecified role unspecified role prey prey n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role bait bait association association false false false 5C ddb1-ezh2 1350258 n/a 1350287 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C cul4b-ddb1-19 1350258 n/a 1350269 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role prey prey association association false false false 5C cul4b-ddb1-20 1350258 n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350287 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 5C cul4b-pten-7 1350258 n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false 5C ezh2-cul4b-1 1350258 n/a 1350285 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role prey prey association association false false false 5C ezh2-ddb1 1350258 n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350287 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait association association false false false 5C suz12-ezh2-2 1350258 n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350287 unspecified role unspecified role bait bait n/a 1350285 unspecified role unspecified role prey prey association association false false false 5C suz12-ezh2-3 1350258 n/a 1350287 unspecified role unspecified role bait bait n/a 1350288 unspecified role unspecified role prey prey n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C suz12-ddb1 1350258 n/a 1350287 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role prey prey association association false false false 5C ezh2-cdkn2a-4 1350258 n/a 1350285 unspecified role unspecified role bait bait n/a 1350288 unspecified role unspecified role prey prey n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C ezh2-cdkn2a-5 1350258 n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait n/a 1350287 unspecified role unspecified role prey prey association association false false false 5C ddb1-cd2a2 1350258 n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350287 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role bait bait association association false false false 5C suz12-cdkn2a-3 1350258 n/a 1350285 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C cul4b-cdkn2a-7 1350258 n/a 1350269 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role prey prey association association false false false 5C cul4b-cdkn2a-8 1350258 n/a 1350287 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C ddb1-cul4b-4 1350258 n/a 1350269 unspecified role unspecified role prey prey n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role bait bait association association false false false 5C ddb1-cd2a2-1 1350258 n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role bait bait n/a 1350285 unspecified role unspecified role prey prey association association false false false 5C suz12-cd2a2 1350258 n/a 1350287 unspecified role unspecified role bait bait n/a 1350288 unspecified role unspecified role prey prey n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C cul4b-ddb1-5 1350259 n/a 1350287 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait n/a 1350289 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey association association false false false Fig 1C top-1, 1F. Depletion of either DDB1 or RbAp46/48 could disrupt the interaction between CUL4B and the PRC2 subunits. ezh2-rnf2 1350259 n/a 1350290 unspecified role unspecified role prey prey n/a 1350291 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait n/a 1350292 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role prey prey association association false false false Fig 1C middle-1 cul4b-ddb1-9 1350259 n/a 1350291 unspecified role unspecified role bait bait n/a 1350285 unspecified role unspecified role prey prey n/a 1350289 unspecified role unspecified role prey prey association association false false false Fig 1C bottom-1 rnf2-eed 1350259 n/a 1350289 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey n/a 1350290 unspecified role unspecified role bait bait association association false false false Fig 1C bottom-2 cul4b-ddb1-10 1350259 n/a 1350285 unspecified role unspecified role prey prey n/a 1350289 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role bait bait association association false false false Fig 1C top-2 ezh2-suz12-3 1350259 n/a 1350291 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role prey prey n/a 1350292 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role prey prey n/a 1350290 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role bait bait association association false false false Fig 1C middle-2 ddb1-jarid2 1350259 n/a 1350293 unspecified role unspecified role prey prey n/a 1350294 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role bait bait association association false false false S1 ddb1-jarid2-1 1350259 n/a 1350294 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait n/a 1350293 unspecified role unspecified role prey prey association association false false false S1-2 ezh2-pten-1 1350260 n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role bait bait association association false false false 5C ezh2-pten-2 1350260 n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait association association false false false 5C cul4b-pten-4 1350260 n/a 1350269 unspecified role unspecified role bait bait n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5 C ezh2-cdkn2a-1 1350260 n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350285 unspecified role unspecified role bait bait association association false false false 5C cul4b-cdkn2a-3 1350260 n/a 1350269 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C cul4b-pten-5 1350260 n/a 1350268 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350287 unspecified role unspecified role bait bait association association false false false 5 C cul4b-cdkn2a-4 1350260 n/a 1350288 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C ddb1-cdkn2a 1350260 n/a 1350287 unspecified role unspecified role bait bait n/a 1350286 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5C ezh2-suz12-4 1350261 n/a 1350287 unspecified role unspecified role bait bait n/a 1350269 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role prey prey n/a 1350290 unspecified role unspecified role prey prey n/a 1350292 unspecified role unspecified role prey prey n/a 1350291 unspecified role unspecified role prey prey association association false false false Fig 1C middle-2 cul4b-ddb1-11 1350261 n/a 1350288 unspecified role unspecified role bait bait n/a 1350289 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey association association false false false Fig 1C top-2 cul4b-ddb1-11 1350261 n/a 1350289 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait n/a 1350285 unspecified role unspecified role prey prey association association false false false Fig 1C top-1 rnf2-eed-1 1350261 n/a 1350289 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey n/a 1350290 unspecified role unspecified role bait bait association association false false false Fig 1C bottom-2 ezh2-rnf2-1 1350261 n/a 1350292 unspecified role unspecified role prey prey n/a 1350290 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait n/a 1350291 unspecified role unspecified role prey prey association association false false false Fig 1C middle-1 cul4b-ddb1-11 1350261 n/a 1350285 unspecified role unspecified role prey prey n/a 1350291 unspecified role unspecified role bait bait n/a 1350289 unspecified role unspecified role prey prey association association false false false Fig 1C bottom-1 cul4b-ddb1-12 1350262 n/a 1350289 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey n/a 1350291 unspecified role unspecified role bait bait association association false false false Fig 1C bottom-1 rnf2-eed-2 1350262 n/a 1350289 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey n/a 1350290 unspecified role unspecified role bait bait association association false false false Fig 1C bottom-2 cul4b-ddb1-12 1350262 n/a 1350287 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role bait bait n/a 1350285 unspecified role unspecified role prey prey n/a 1350289 unspecified role unspecified role prey prey association association false false false Fig 1C top-2 ezh2-suz12-5 1350262 n/a 1350291 unspecified role unspecified role prey prey n/a 1350290 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role prey prey n/a 1350292 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role bait bait n/a 1350288 unspecified role unspecified role prey prey association association false false false Fig 1C middle-2 cul4b-ddb1-12 1350262 n/a 1350285 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role prey prey n/a 1350289 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait association association false false false Fig 1C top-1 ezh2-rnf2-2 1350262 n/a 1350292 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role bait bait n/a 1350290 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role prey prey n/a 1350291 unspecified role unspecified role prey prey association association false false false Fig 1C middle-1 cul4b-ddb1-4 1350263 n/a 1350287 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey n/a 1350289 unspecified role unspecified role prey prey n/a 1350269 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350295 unspecified role unspecified role prey prey n/a 1350292 unspecified role unspecified role prey prey association association false false false Fig 1B,2E. cul4b-ddb1-17 1350263 n/a 1350269 unspecified role unspecified role bait bait did_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350289 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey n/a 1350287 unspecified role unspecified role prey prey n/a 1350295 unspecified role unspecified role prey prey n/a 1350288 unspecified role unspecified role prey prey association association false false false Fig 2E. The DID domain is responsible for the formation of the CRL4B-PRC2 machinery, while the Cullin domain is responsible for the binding of the ring finger protein ROC1 (along with E1/E2/E3). cul4b-ddb1-18 1350263 n/a 1350269 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cullin_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1350292 unspecified role unspecified role prey prey association association false false false Fig 2Eii. The DID domain is responsible for the formation of the CRL4B-PRC2 machinery, while the Cullin domain is responsible for the binding of the ring finger protein ROC1 (along with E1/E2/E3). ddb1-cul4b-1 1350264 n/a 1350269 unspecified role unspecified role prey prey in vitro In vitro n/a 1350288 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 1E ddb1-rbbp7 1350264 n/a 1350288 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1350295 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false Fig 1E ddb1-rbbp4 1350264 n/a 1350288 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1350296 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false Fig 1E ddb1-gal4 1350265 n/a 1350297 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role bait bait n/a 1350269 unspecified role unspecified role ancillary ancillary did_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false association association false false false 2D-1 The artificial recruitment of CUL4B resulted in the recruitment of DDB1 and EXH2. ddb1-gal4-1 1350265 n/a 1350269 unspecified role unspecified role ancillary ancillary did_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false cullin_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1350285 unspecified role unspecified role bait bait n/a 1350297 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2D-2 The artificial recruitment of CUL4B resulted in the recruitment of DDB1 and EXH2. cul4b-ddb1-14 1350265 n/a 1350297 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350269 unspecified role unspecified role bait bait association association false false false 2D-3 cul4b-cops3 1350266 n/a 1350269 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1350288 unspecified role unspecified role prey prey n/a 1350298 unspecified role unspecified role prey prey n/a 1350285 unspecified role unspecified role prey prey n/a 1350299 unspecified role unspecified role prey prey n/a 1350300 unspecified role unspecified role prey prey n/a 1350295 unspecified role unspecified role prey prey n/a 1350301 unspecified role unspecified role prey prey n/a 1350289 unspecified role unspecified role prey prey n/a 1350302 unspecified role unspecified role prey prey n/a 1350303 unspecified role unspecified role prey prey association association false false false Table S1, Fig1A. cul4b-ddb1-13 1350267 n/a 1350269 unspecified role unspecified role neutral component neutral component n/a 1350285 unspecified role unspecified role neutral component neutral component n/a 1350289 unspecified role unspecified role neutral component neutral component n/a 1350292 unspecified role unspecified role neutral component neutral component n/a 1350287 unspecified role unspecified role neutral component neutral component n/a 1350288 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig 1D-1