IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2012a-1 The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. human-be3 Human BE3-esophageal adenocarcinoma cell line be3 Human BE3 - esophageal adenocarcinoma cell line Barrett's esophagus associated esophageal adenocarcinoma from distal esophagus. Expresses: pIκκBeta (S181) pTSC1(S511) p36K1(T389) members of the inflammation associated signaling pathway. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation wang-2012a-2 The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. human-skgt4 Human SKGT4 skgt4 Human SKGT4 - esophageal adenocarcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation wang-2012a-3 The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. human-be3 Human BE3-esophageal adenocarcinoma cell line be3 Human BE3 - esophageal adenocarcinoma cell line Barrett's esophagus associated esophageal adenocarcinoma from distal esophagus. Expresses: pIκκBeta (S181) pTSC1(S511) p36K1(T389) members of the inflammation associated signaling pathway. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation wang-2012a-4 The Crosstalk of mTOR/S6K1 and Hedgehog Pathways. in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Wang Y., Ding Q., Yen C.J., Xia W., Izzo J.G., Lang J.Y., Li C.W., Hsu J.L., Miller S.A., Wang X., Lee D.F., Hsu J.M., Huo L., Labaff A.M., Liu D., Huang T.H., Lai C.C., Tsai F.J., Chang W.C., Chen C.H., Wu T.T., Buttar N.S., Wang K.K., Wu Y., Wang H., Ajani J., Hung M.C. Cancer - Interactions investigated in the context of cancer After transfection with HA-S6K1 or mutants, Be3 cells were lysed and immunoprecipitated with anti-HA antibody. The authors have confirmed that the constructs "HA-S6K1" and mutants, GST-Gli1F1, Gli1S84A/E, Flag-Gli1S84A and Flag-Gli1 WT are all of human origin. mhung@mdanderson.org Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation ks6b1_human Ribosomal protein S6 kinase beta-1 RPS6KB1 STK14A Ribosomal protein S6 kinase I p70 ribosomal S6 kinase alpha 70 kDa ribosomal protein S6 kinase 1 Serine/threonine-protein kinase 14A protein protein human Homo sapiens Human MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL gli1_human Zinc finger protein GLI1 GLI1 GLI Glioma-associated oncogene Oncogene GLI protein protein human Homo sapiens Human MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA sufu_human Suppressor of fused homolog SUFU UNQ650/PRO1280 protein protein human Homo sapiens Human MAELRPSGAPGPTAPPAPGPTAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDYVSMYRNVGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICIGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH p23443-2 Ribosomal protein S6 kinase beta-1 Ribosomal protein S6 kinase I Serine/threonine-protein kinase 14A p70 ribosomal S6 kinase alpha RPS6KB1 STK14A 70 kDa ribosomal protein S6 kinase 1 protein protein human Homo sapiens Human MAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL gli1-rps6kb1-1 1283835 n/a 1283839 unspecified role unspecified role prey prey n/a 1283840 unspecified role unspecified role bait bait physical association physical association false false false Fig 2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin rps6kb1-gli1-1 1283835 n/a 1283839 unspecified role unspecified role bait bait n/a 1283840 unspecified role unspecified role prey prey physical association physical association false false false Fig S2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin gli1-sufu 1283835 n/a 1283840 unspecified role unspecified role bait bait n/a 1283841 unspecified role unspecified role prey prey physical association physical association false false false Fig 5E sufu-gli1-1 1283835 n/a 1283841 unspecified role unspecified role bait bait n/a 1283840 unspecified role unspecified role prey prey P08151:p.Ser84Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 5E sufu-gli1-2 1283835 n/a 1283840 unspecified role unspecified role prey prey n/a 1283841 unspecified role unspecified role bait bait physical association physical association false false false Fig 5F Rapamycin TNF-alpha and S6K1. Both TNF-a treatment and S6K1 ectopic expression decreased the binding between SuFu and Gli1, which was fully reversed by rapamycin. sufu-gli1-3 1283835 n/a 1283841 unspecified role unspecified role bait bait n/a 1283840 unspecified role unspecified role prey prey P08151:p.Ser84Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig S5K gli1-rps6kb1-2 1283836 n/a 1283839 unspecified role unspecified role prey prey n/a 1283840 unspecified role unspecified role bait bait physical association physical association false false false Fig 2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin rps6kb1-gli1 1283836 n/a 1283839 unspecified role unspecified role bait bait n/a 1283840 unspecified role unspecified role prey prey physical association physical association false false false Fig S2A TNF-alpha, no interactions were found between Gli1 and mTOR pathway components in the absence of TNFalpha. Rapamycin rps6kb1-gli1-2 1283837 n/a 1283842 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P23443-2:p.Thr389Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P23443-2:p.Thr389Glu mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false n/a 1283840 unspecified role unspecified role prey prey physical association physical association false false false Fig 2B Rapamycin could inhibit the activity of S6K1, but not that of (constitutively activated) S6K1T389E. gli1-sufu-7 1283837 n/a 1283840 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1283841 unspecified role unspecified role prey prey physical association physical association false false false Fig S5K gli1-rps6kb1-3 1283838 n/a 1283840 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P08151:p.Ser84Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1283842 enzyme enzyme neutral component neutral component P23443-2:p.Thr389Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-be3 Human BE3-esophageal adenocarcinoma cell line be3 Human BE3 - esophageal adenocarcinoma cell line Barrett's esophagus associated esophageal adenocarcinoma from distal esophagus. Expresses: pIκκBeta (S181) pTSC1(S511) p36K1(T389) members of the inflammation associated signaling pathway. phosphorylation phosphorylation reaction phosphorylation false false false Fig 3B, 3D Rapamycin could inhibit the activity of S6K1, but not that of (constitutively activated) S6K1T389E.