IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ vermeer-2012-6 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunostaining immunostaining Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-8 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org The anti-ErbB2 antibody utilized for precipitation recognizes both wildtype (Human) ErbB2 and mNeuNT, but as Hek293 cells express little endogenous Erbb2 there is not a detection problem. PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-9 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-1 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-mda_mb_468 Homo sapiens MDA-MB-468 mda_mb_468 MDA-MB-468 breast adenocarcinoma Species: human, Black; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-2 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-hacat Homo sapiens immortalised keratinocytes hacat human immortalised keratinocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-3 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-4 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-bt474 Homo sapiens breast ductal carcinoma cell line bt474 Human breast ductal carcinoma cell line BT474 Human breast ductal carcinoma cell line established from a solid, invasive ductal carcinoma of the breast obtained from a 60-year-old woman. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-5 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-hcc-1954 HCC-1954 Ductal Carcinoma cell line hcc-1954 HCC-1954 Human Breast (Ductal) Carcinoma cell line ATCC Number = CRL-2338 HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. ER-, PR-, ERBB2+. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer The image legend refers to this cell line as HCC-1953, but this cell line is not referred to again in this paper, or elsewhere. Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation vermeer-2012-7 ErbB2, EphrinB1, Src Kinase and PTPN13 Signaling Complex Regulates MAP Kinase Signaling in Human Cancers. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vermeer P.D., Bell M., Lee K., Vermeer D.W., Wieking B.G., Bilal E., Bhanot G., Drapkin R.I., Ganesan S., Klingelhutz A.J., Hendriks W.J., Lee J.H. Cancer - Interactions investigated in the context of cancer Source of mNeuNT was found in Ref 23. Transfected Wild type Erbb2 was deduced to be Human from primer sequences used. John.Lee@SanfordHealth.org PLoS ONE 2012 Only protein-protein interactions imex curation efnb1_human Ephrin-B1 EPH-related receptor tyrosine kinase ligand 2 EFL3 EFNB1 EFL-3 EPLG2 LERK2 ELK ligand protein protein human Homo sapiens Human MARPGQRWLGKWLVAMVVWALCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDGFFNSKVALFAAVGAGCVIFLLIIIFLTVLLLKLRKRHRKHTQQRAAALSLSTLASPKGGSGTAGTEPSDIIIPLRTTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV erbb2_mouse Receptor tyrosine-protein kinase erbB-2 Erbb2 Kiaa3023 Neu Proto-oncogene Neu Proto-oncogene c-ErbB-2 p185erbB2 protein protein mouse Mus musculus Mouse MELAAWCRWGFLLALLSPGAAGTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPANASLSFLQDIQEVQGYMLIAHNRVKHVPLQRLRIVRGTQLFEDKYALAVLDNRDPLDNVTTAAPGRTPEGLRELQLRSLTEILKGGVLIRGNPQLCYQDMVLWKDVLRKNNQLAPVDMDTNRSRACPPCAPTCKDNHCWGESPEDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALITYNTDTFESMLNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEVTAEDGTQRCEKCSKPCAGVCYGLGMEHLRGARAITSDNIQEFAGCKKIFGSLAFLPESFDGNPSSGVAPLKPEHLQVFETLEEITGYLYISAWPESFQDLSVFQNLRVIRGRILHDGAYSLTLQGLGIHSLGLRSLRELGSGLALIHRNTHLCFVNTVPWDQLFRNPHQALLHSGNRPEEACGLEGLVCNSLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVWKGLPREYVRGKHCLPCHPECQPQNSSETCYGSEADQCEACAHYKDSSSCVARCPSGVKPDLSYMPIWKYPDEEGICQPCPINCTHSCVDLDERGCPAEQRASPVTFIIATVVGVLLFLIIVVVIGILIKRRRQKIRKYTMRRLLQETELVEPLTPSGAVPNQAQMRILKETELRKLKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVREHRGRLGSQDLLNWCVQIAKGMSYLEEVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPSSPMDSTFYRSLLEDDDMGELVDAEEYLVPQQGFFSPDPALGTGSTAHRRHRSSSARSGGGELTLGLEPSEEEPPRSPLAPSEGAGSDVFDGDLAVGVTKGLQSLSPHDLSPLQRYSEDPTLPLPPETDGYVAPLACSPQPEYVNQPEVRPQSPLTPEGPPPPIRPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLAPRAGTASQPHPSPAFSPAFDNLYYWDQNSSEQGPPPSTFEGTPTAENPEYLGLDVPV erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV src_human Proto-oncogene tyrosine-protein kinase Src pp60c-src SRC SRC1 Proto-oncogene c-Src protein protein human Homo sapiens Human MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL efnb1-erbb2 1267964 n/a 1267973 unspecified role unspecified role neutral component neutral component n/a 1267974 unspecified role unspecified role neutral component neutral component P70424:p.Val660Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 2F Only expression of mNeuNT results in phosphorylated EphrinB present at the cell surface. efnb1-erbb2-3 1267964 n/a 1267973 unspecified role unspecified role neutral component neutral component n/a 1267975 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 4D Endogenous EphrinB1 associated with all ErbB2 mutants. efnb1-erbb2-4 1267964 n/a 1267975 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1267973 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 4D All constructs localised to the membrane. erbb2-efnb1-6 1267965 n/a 1267975 unspecified role unspecified role bait bait n/a 1267973 unspecified role unspecified role prey prey physical association physical association false false false 2E EphrinB1 associated with transfected ErbB2. erbb2-efnb1-7 1267965 n/a 1267973 unspecified role unspecified role prey prey n/a 1267974 unspecified role unspecified role bait bait P70424:p.Val660Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2E Expression of mNeuNT with EphrinB1 increases the amount of EphrinB1 precipitated, and it's phosphorylation. phosphatase null mutant PTPN13C/S erbb2-efnb1-8 1267965 n/a 1267976 unspecified role unspecified role prey prey n/a 1267974 unspecified role unspecified role bait bait P70424:p.Val660Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3A, 3B. PP2, a potent Src inhibitor. efnb1-erbb2-1 1267966 n/a 1267975 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1267973 unspecified role unspecified role bait bait physical association physical association false false false 4B All constructs localized to the membrane. erbb2-efnb1-9 1267966 n/a 1267975 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1267973 unspecified role unspecified role prey prey n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 4C Erbb2 associated with all Ephrin mutants. erbb2-efnb1 1267967 n/a 1267973 unspecified role unspecified role prey prey n/a 1267975 unspecified role unspecified role bait bait physical association physical association false false false 1E EphrinB1 associated with ErbB2 in lysates from shPTPN13 cells. erbb2-efnb1-1 1267968 n/a 1267973 unspecified role unspecified role prey prey n/a 1267975 unspecified role unspecified role bait bait physical association physical association false false false 2A knock-down of PTPN13 in HaCaT cells (shPTPN13) enhanced pull-down of EphrinB1 with ErbB2. erbb2-efnb1-2 1267969 n/a 1267975 unspecified role unspecified role bait bait n/a 1267973 unspecified role unspecified role prey prey physical association physical association false false false 2A, 2C. erbb2-efnb1-3 1267970 n/a 1267973 unspecified role unspecified role prey prey n/a 1267975 unspecified role unspecified role bait bait physical association physical association false false false 2A, 2C. erbb2-efnb1-4 1267971 n/a 1267975 unspecified role unspecified role bait bait n/a 1267973 unspecified role unspecified role prey prey physical association physical association false false false 2A erbb2-efnb1-5 1267972 n/a 1267975 unspecified role unspecified role bait bait n/a 1267973 unspecified role unspecified role prey prey physical association physical association false false false 2C EphrinB1 associated with ErbB2 in lysates from shPTPN13 cells.