I2D I2D huang-2012a-1 Tumor suppressor Alpha B-crystallin (CRYAB) associates with the cadherin/catenin adherens junction and impairs NPC progression-associated properties. human-hone1 Human nasopharyngeal carcinoma cell line hone1 Human nasopharyngeal carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang Z., Cheng Y., Chiu P.M., Cheung F.M., Nicholls J.M., Kwong D.L., Lee A.W., Zabarovsky E.R., Stanbridge E.J., Lung H.L., Lung M.L. Cancer - Interactions investigated in the context of cancer The HONE1-2 cell line expressed the tetracycline transcriptional activator (tTA) as per reference Protopopov et al. 2002 (PMID:12124982). pETE-Bsd-CRYAB was transfected into these cells to generate stable transfectants CRYAB42 and CRYAB46. Immunoprecipitations were carried out on these cell lines. hllung2@hku.hk, mliling@hku.hk Oncogene (0950-9232) 2012 Curated by: I2D - Interologous Interaction Database huang-2012a-2 Tumor suppressor Alpha B-crystallin (CRYAB) associates with the cadherin/catenin adherens junction and impairs NPC progression-associated properties. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Huang Z., Cheng Y., Chiu P.M., Cheung F.M., Nicholls J.M., Kwong D.L., Lee A.W., Zabarovsky E.R., Stanbridge E.J., Lung H.L., Lung M.L. Cancer - Interactions investigated in the context of cancer The HONE1-2 cell line expressed the tetracycline transcriptional activator (tTA) as per reference Protopopov et al. 2002 (PMID:12124982). pETE-Bsd-CRYAB was transfected into these cells to generate stable transfectants CRYAB42 and CRYAB46. Immunoprecipitations were carried out on these cell lines. Source of CRYAB construct taken from PMID: 14594798 hllung2@hku.hk, mliling@hku.hk Oncogene (0950-9232) 2012 Curated by: I2D - Interologous Interaction Database cadh1_human Cadherin-1 Epithelial cadherin Uvomorulin CAM 120/80 CDH1 CDHE UVO protein protein human Homo sapiens Human MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFEDCTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNTVGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPAILGILGGILALLILILLLLLFLRRRAVVKEPLLPPEDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPRYLPRPANPDEIGNFIDENLKAADTDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDKDQDYDYLNEWGNRFKKLADMYGGGEDD cryab_human Alpha-crystallin B chain CRYAB CRYA2 Renal carcinoma antigen NY-REN-27 Alpha(B)-crystallin Rosenthal fiber component Heat shock protein beta-5 HSPB5 protein protein human Homo sapiens Human MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFPTSTSLSPFYLRPPSFLRAPSWFDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK AE08BED46B7849CB ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL cryab-cdh1-1 1316734 n/a 1316736 unspecified role unspecified role prey prey n/a 1316737 unspecified role unspecified role bait bait n/a 1316738 unspecified role unspecified role prey prey physical association physical association false false false 6a ctnnb1-cdh1-12 1316734 n/a 1316738 unspecified role unspecified role bait bait n/a 1316737 unspecified role unspecified role prey prey n/a 1316736 unspecified role unspecified role prey prey physical association physical association false false false 6b cryab-cdh1-2 1316735 n/a 1316736 unspecified role unspecified role prey prey human-hone1 Human nasopharyngeal carcinoma cell line hone1 Human nasopharyngeal carcinoma cell line n/a 1316738 unspecified role unspecified role prey prey human-hone1 Human nasopharyngeal carcinoma cell line hone1 Human nasopharyngeal carcinoma cell line n/a 1316737 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 6c-d