IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ scott-2011-1 Proinvasion metastasis drivers in early-stage melanoma are oncogenes. human-wm115 Human WM115 Melanoma cell line wm115 WM115 Human melanoma cell line http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=CRL-1675&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Scott K.L., Nogueira C., Heffernan T.P., van Doorn R., Dhakal S., Hanna J.A., Min C., Jaskelioff M., Xiao Y., Wu C.J., Cameron L.A., Perry S.R., Zeid R., Feinberg T., Kim M., Vande Woude G., Granter S.R., Bosenberg M., Chu G.C., Depinho R.A., Rimm D.L., Chin L. Cancer - Interactions investigated in the context of cancer lynda_chin@dfci.harvard.edu Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation scott-2011-2 Proinvasion metastasis drivers in early-stage melanoma are oncogenes. human-hmel468 Human HMEL468 hmel468 HMEL468 (PMEL/hTERT/CDK4(R24C)/p53DD), a TERT-immortalized primary human melanocyte line engineered to express BRAFV600E (Garraway et al., 2005). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Scott K.L., Nogueira C., Heffernan T.P., van Doorn R., Dhakal S., Hanna J.A., Min C., Jaskelioff M., Xiao Y., Wu C.J., Cameron L.A., Perry S.R., Zeid R., Feinberg T., Kim M., Vande Woude G., Granter S.R., Bosenberg M., Chu G.C., Depinho R.A., Rimm D.L., Chin L. Cancer - Interactions investigated in the context of cancer lynda_chin@dfci.harvard.edu Cancer Cell (1535-6108) 2011 Only protein-protein interactions imex curation fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC pxn-ptk2-3 1168395 n/a 1168397 unspecified role unspecified role prey prey n/a 1168398 unspecified role unspecified role bait bait physical association physical association false false false Fig 6C A faint band can be seen, the intensity of which is reduced In the presence of overexpressed ACP5. ptk2-pxn-1 1168395 n/a 1168398 unspecified role unspecified role prey prey n/a 1168397 unspecified role unspecified role bait bait physical association physical association false false false Fig 6C pxn-ptk2-4 1168396 n/a 1168398 unspecified role unspecified role bait bait n/a 1168397 unspecified role unspecified role prey prey physical association physical association false false false Fig 6C ptk2-pxn-2 1168396 n/a 1168398 unspecified role unspecified role prey prey n/a 1168397 unspecified role unspecified role bait bait physical association physical association false false false Fig 6C