IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tanikawa-2011-5 Multifunctional transcription factor TFII-I is an activator of BRCA1 function. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Tanikawa M., Wada-Hiraike O., Nakagawa S., Shirane A., Hiraike H., Koyama S., Miyamoto Y., Sone K., Tsuruga T., Nagasaka K., Matsumoto Y., Ikeda Y., Shoji K., Oda K., Fukuhara H., Nakagawa K., Kato S., Yano T., Taketani Y. Cancer - Interactions investigated in the context of cancer osamu.hiraike@gmail.com Br. J. Cancer (0007-0920) 2011 Only protein-protein interactions imex curation tanikawa-2011-2 Multifunctional transcription factor TFII-I is an activator of BRCA1 function. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tanikawa M., Wada-Hiraike O., Nakagawa S., Shirane A., Hiraike H., Koyama S., Miyamoto Y., Sone K., Tsuruga T., Nagasaka K., Matsumoto Y., Ikeda Y., Shoji K., Oda K., Fukuhara H., Nakagawa K., Kato S., Yano T., Taketani Y. Cancer - Interactions investigated in the context of cancer osamu.hiraike@gmail.com Br. J. Cancer (0007-0920) 2011 Only protein-protein interactions imex curation tanikawa-2011-1 Multifunctional transcription factor TFII-I is an activator of BRCA1 function. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Tanikawa M., Wada-Hiraike O., Nakagawa S., Shirane A., Hiraike H., Koyama S., Miyamoto Y., Sone K., Tsuruga T., Nagasaka K., Matsumoto Y., Ikeda Y., Shoji K., Oda K., Fukuhara H., Nakagawa K., Kato S., Yano T., Taketani Y. Cancer - Interactions investigated in the context of cancer osamu.hiraike@gmail.com Br. J. Cancer (0007-0920) 2011 Only protein-protein interactions imex curation tanikawa-2011-3 Multifunctional transcription factor TFII-I is an activator of BRCA1 function. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tanikawa M., Wada-Hiraike O., Nakagawa S., Shirane A., Hiraike H., Koyama S., Miyamoto Y., Sone K., Tsuruga T., Nagasaka K., Matsumoto Y., Ikeda Y., Shoji K., Oda K., Fukuhara H., Nakagawa K., Kato S., Yano T., Taketani Y. Cancer - Interactions investigated in the context of cancer Origin of TFII-I was deduced by blatting PCR primers as given in Cheriyath et al., JBC, 2000: also by the sequence length of the isoforms given in Cheriyath and Roy, JBC, 2001. osamu.hiraike@gmail.com Br. J. Cancer (0007-0920) 2011 Only protein-protein interactions imex curation tanikawa-2011-4 Multifunctional transcription factor TFII-I is an activator of BRCA1 function. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Tanikawa M., Wada-Hiraike O., Nakagawa S., Shirane A., Hiraike H., Koyama S., Miyamoto Y., Sone K., Tsuruga T., Nagasaka K., Matsumoto Y., Ikeda Y., Shoji K., Oda K., Fukuhara H., Nakagawa K., Kato S., Yano T., Taketani Y. Cancer - Interactions investigated in the context of cancer osamu.hiraike@gmail.com Br. J. Cancer (0007-0920) 2011 Only protein-protein interactions imex curation cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY gtf2i_human General transcription factor II-I GTF2I BAP135 WBSCR6 Bruton tyrosine kinase-associated protein 135 SRF-Phox1-interacting protein Williams-Beuren syndrome chromosomal region 6 protein protein protein human Homo sapiens Human MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQAGPSETDDVDEKQPLSKPLQGSHHSSEGNEGTEMEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPSKRPKANELPQPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVISYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQSESEGPVIQESAEPSQLEVPATEEIKETDGSSQIKQEPDPTW sir1_human_gene SIRT1 Human Gene gene gene human Homo sapiens Human brca1-cdkn1a 1312280 n/a 1312285 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1312286 unspecified role unspecified role bait bait association association false false false Fig 4C brca1-cdkn1a-1 1312280 n/a 1312287 unspecified role unspecified role bait bait n/a 1312285 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 4C gtf2i-cdkn1a 1312280 n/a 1312288 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1312287 unspecified role unspecified role bait bait association association false false false Fig 4C gtf2i-sir1 1312280 n/a 1312286 unspecified role unspecified role bait bait n/a 1312288 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 4C brca1-gtf2i-1 1312281 n/a 1312287 unspecified role unspecified role prey prey n/a 1312286 unspecified role unspecified role bait bait physical association physical association false false false Fig 1B-1 gtf2i-brca1 1312281 n/a 1312286 unspecified role unspecified role prey prey n/a 1312287 unspecified role unspecified role bait bait physical association physical association false false false Fig 1B-3 brca1-gtf2i 1312282 n/a 1312287 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1312286 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 1A brca1-gtf2i-2 1312283 n/a 1312287 unspecified role unspecified role prey prey r5_and_r6_regions required to bind necessary binding region required to bind deletion analysis deletion analysis range range certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1312286 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 1C/D Using the cytoplasmic fraction of COS7 cells, TFII-I was not co-immunoprecipitated with BRCA1. brca1-gtf2i-4 1312284 n/a 1312286 unspecified role unspecified role neutral component neutral component n/a 1312287 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 2 Cells were treated with 8-Gy gamma-radiation to damage DNA