I2D I2D bist-2011-3 Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database bist-2011-4 Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database bist-2011-5 Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database bist-2011-1 Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database bist-2011-2 Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. linalim@nus.edu.sg Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database bist-2011-6 Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-κB: implication in breast cancer metastasis. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Bist P., Leow S.C., Phua Q.H., Shu S., Zhuang Q., Loh W.T., Nguyen T.H., Zhou J.B., Hooi S.C., Lim L.H. Cancer - Interactions investigated in the context of cancer A Sephacryl 200 column. Author confirms 1% formaldehyde was used for ChIP experiments. linalim@nus.edu.sg Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database nemo_human NF-kappa-B essential modulator IKBKG FIP3 NEMO NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 FIP-3 protein protein human Homo sapiens Human MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE anxa1_human Annexin A1 ANXA1 ANX1 LPC1 Annexin-1 Annexin I Lipocortin I Calpactin II Chromobindin-9 p35 Phospholipase A2 inhibitory protein Calpactin-2 protein protein human Homo sapiens Human MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN ripk1_human Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP Cell death protein RIP RIP1 Receptor-interacting protein 1 protein protein human Homo sapiens Human MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS cxcr4_human_gene Human CXCR4 gene gene gene human Homo sapiens Human anxa1-nemo 1167884 n/a 1167890 unspecified role unspecified role prey prey n/a 1167891 unspecified role unspecified role bait bait physical association physical association false false false 2e Immunoprecipitations were carried out on gel-filtration elution fractions of MCF7 cells transfected with ANXA1. A Sephacryl 200 column was used. NEMO and ANXA1 are coimmunoprecipitated in fractions 5-9. ripk1-anxa1 1167884 n/a 1167891 unspecified role unspecified role prey prey n/a 1167892 unspecified role unspecified role bait bait physical association physical association false false false 3a nemo-anxa1 1167884 n/a 1167890 unspecified role unspecified role bait bait n/a 1167891 unspecified role unspecified role prey prey physical association physical association false false false 3a anxa1-rela 1167884 n/a 1167891 unspecified role unspecified role bait bait n/a 1167893 unspecified role unspecified role prey prey physical association physical association false false false 3d nemo-anxa1-1 1167885 n/a 1167890 unspecified role unspecified role bait bait n/a 1167891 unspecified role unspecified role prey prey physical association physical association false false false 3b ripk1-anxa1-1 1167885 n/a 1167891 unspecified role unspecified role prey prey n/a 1167892 unspecified role unspecified role bait bait physical association physical association false false false 3b ripk1-ikbkg 1167885 n/a 1167890 unspecified role unspecified role prey prey n/a 1167892 unspecified role unspecified role bait bait physical association physical association false false false 3c ikbkg-ripk1-4 1167885 n/a 1167890 unspecified role unspecified role bait bait n/a 1167892 unspecified role unspecified role prey prey physical association physical association false false false 3c anxa1-ikbkg-3 1167886 n/a 1167890 unspecified role unspecified role neutral component neutral component n/a 1167891 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3e anxa1-ripk1 1167886 n/a 1167892 unspecified role unspecified role neutral component neutral component n/a 1167891 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3e anxa1-ikbkg 1167887 n/a 1167891 unspecified role unspecified role neutral component neutral component n/a 1167890 unspecified role unspecified role neutral component neutral component n/a 1167892 unspecified role unspecified role neutral component neutral component physical association physical association false false false 2b,c In controls, ANXA1 is observed only in high molecular weight complexes. Overexpression of ANXA1 results in both high and low molecular weight complexes for ANXA1, NEMO and RIP suggesting that ANXA1 overexpression encourages oligomerization of NEMO and RIP. anxa1-ikbkg-1 1167888 n/a 1167891 unspecified role unspecified role neutral component neutral component n/a 1167890 unspecified role unspecified role neutral component neutral component n/a 1167892 unspecified role unspecified role neutral component neutral component physical association physical association false false false 2d rela-cxcr4 1167889 n/a 1167893 unspecified role unspecified role bait bait n/a 1167894 unspecified role unspecified role prey prey association association false false false 6f SDF1 alpha (stromal-derived factor 1 alpha or CXCL12) recruits NF-kappaB to the CXCR4 promoter in MCF7 cells. Notably, NF-kappaB was recruited to CXCR4 promoter in MCF7-V5 cells without SDF1alpha treatment.