IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fernandez-2010-1 VRK2 inhibits MAPK signaling and inversely correlates with ErbB2 in human breast cancer. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Fernandez IF., Blanco S., Lozano J., Lazo PA. Cancer - Interactions investigated in the context of cancer 2010-8-17: Lazo PA, Centro de Investigacion del Cancer, CSIC- Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. plazozbi@usal.es Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fernandez-2010-2 VRK2 inhibits MAPK signaling and inversely correlates with ErbB2 in human breast cancer. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Fernandez IF., Blanco S., Lozano J., Lazo PA. Cancer - Interactions investigated in the context of cancer 2010-8-17: Lazo PA, Centro de Investigacion del Cancer, CSIC- Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. plazozbi@usal.es Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fernandez-2010-3 VRK2 inhibits MAPK signaling and inversely correlates with ErbB2 in human breast cancer. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Fernandez IF., Blanco S., Lozano J., Lazo PA. Cancer - Interactions investigated in the context of cancer 2010-8-17: Lazo PA, Centro de Investigacion del Cancer, CSIC- Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. plazozbi@usal.es Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fernandez-2010-4 VRK2 inhibits MAPK signaling and inversely correlates with ErbB2 in human breast cancer. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pull down pull down western blot western blot Immuno blot Immuno blot Fernandez IF., Blanco S., Lozano J., Lazo PA. Cancer - Interactions investigated in the context of cancer 2010-8-17: Lazo PA, Centro de Investigacion del Cancer, CSIC- Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. plazozbi@usal.es Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fernandez-2010-5 VRK2 inhibits MAPK signaling and inversely correlates with ErbB2 in human breast cancer. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fernandez IF., Blanco S., Lozano J., Lazo PA. Cancer - Interactions investigated in the context of cancer 2010-8-17: Lazo PA, Centro de Investigacion del Cancer, CSIC- Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. plazozbi@usal.es Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fernandez-2010-6 VRK2 inhibits MAPK signaling and inversely correlates with ErbB2 in human breast cancer. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Fernandez IF., Blanco S., Lozano J., Lazo PA. Cancer - Interactions investigated in the context of cancer 2010-8-17: Lazo PA, Centro de Investigacion del Cancer, CSIC- Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. plazozbi@usal.es Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fernandez-2010-7 VRK2 inhibits MAPK signaling and inversely correlates with ErbB2 in human breast cancer. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down western blot western blot Immuno blot Immuno blot Fernandez IF., Blanco S., Lozano J., Lazo PA. Cancer - Interactions investigated in the context of cancer 2010-8-17: Lazo PA, Centro de Investigacion del Cancer, CSIC- Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain. plazozbi@usal.es Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation calx_human Calnexin CANX Major histocompatibility complex class I antigen-binding protein p88 p90 IP90 protein protein human Homo sapiens Human MEGKWLLCMLLVLGTAIVEAHDGHDDDVIDIEDDLDDVIEEVEDSKPDTTAPPSSPKVTYKAPVPTGEVYFADSFDRGTLSGWILSKAKKDDTDDEIAKYDGKWEVEEMKESKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTPELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGIYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDPEDRKPEDWDERPKIPDPEAVKPDDWDEDAPAKIPDEEATKPEGWLDDEPEYVPDPDAEKPEDWDEDMDGEWEAPQIANPRCESAPGCGVWQRPVIDNPNYKGKWKPPMIDNPSYQGIWKPRKIPNPDFFEDLEPFRMTPFSAIGLELWSMTSDIFFDNFIICADRRIVDDWANDGWGLKKAADGAAEPGVVGQMIEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQTSGMEYKKTDAPQPDVKEEEEEKEEEKDKGDEEEEGEEKLEEKQKSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE ksr1_human Kinase suppressor of Ras 1 KSR1 KSR protein protein human Homo sapiens Human MDRAALRAAAMGEKKEGGGGGDAAAAEGGAGAAASRALQQCGQLQKLIDISIGSLRGLRTKCAVSNDLTQQEIRTLEAKLVRYICKQRQCKLSVAPGERTPELNSYPRFSDWLYTFNVRPEVVQEIPRDLTLDALLEMNEAKVKETLRRCGASGDECGRLQYALTCLRKVTGLGGEHKEDSSWSSLDARRESGSGPSTDTLSAASLPWPPGSSQLGRAGNSAQGPRSISVSALPASDSPTPSFSEGLSDTCIPLHASGRLTPRALHSFITPPTTPQLRRHTKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVSSMRFDLSHGSPQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIVREMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIGSGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLNRRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM vrk2_human Serine/threonine-protein kinase VRK2 VRK2 Vaccinia-related kinase 2 protein protein human Homo sapiens Human MPPKRNEKYKLPIPFPEGKVLDDMEGNQWVLGKKIGSGGFGLIYLAFPTNKPEKDARHVVKVEYQENGPLFSELKFYQRVAKKDCIKKWIERKQLDYLGIPLFYGSGLTEFKGRSYRFMVMERLGIDLQKISGQNGTFKKSTVLQLGIRMLDVLEYIHENEYVHGDIKAANLLLGYKNPDQVYLADYGLSYRYCPNGNHKQYQENPRKGHNGTIEFTSLDAHKGVALSRRSDVEILGYCMLRWLCGKLPWEQNLKDPVAVQTAKTNLLDELPQSVLKWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINVHTPNSQKVDSQKAATKQVNKAHNRLIEKKVHSERSAESCATWKVQKEEKLIGLMNNEAAQESTRRRQKYQESQEPLNEVNSFPQKISYTQFPNSFYEPHQDFTSPDIFKKSRSPSWYKYTSTVSTGITDLESSTGLWPTISQFTLSEETNADVYYYRIIIPVLLMLVFLALFFL mp2k1_human Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAPK/ERK kinase 1 MAP2K1 MEK1 PRKMK1 protein protein human Homo sapiens Human MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV ksr1-calnexin 976616 n/a 976623 unspecified role unspecified role neutral component neutral component n/a 976624 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6A vrk2-ksr1 976616 n/a 976624 unspecified role unspecified role neutral component neutral component n/a 976625 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6B ksr1-calnexin-1 976617 n/a 976623 unspecified role unspecified role neutral component neutral component n/a 976624 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6A ksr1-calnexin-2 976618 n/a 976624 unspecified role unspecified role neutral component neutral component n/a 976623 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6A vrk2a-mek 976619 n/a 976626 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 976625 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 7B ksr1-vrk2a 976620 n/a 976625 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 976624 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 7A mek1-vrk2a 976621 n/a 976626 unspecified role unspecified role bait bait n/a 976625 unspecified role unspecified role prey prey physical association physical association false false false 7C vrk2a-ksr1 976622 n/a 976624 unspecified role unspecified role prey prey n/a 976625 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 7A