IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xiao-2009-1 Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. mouse-ba_f3 Mouse pro B cells IL-3 dependent mouse-baf3 EBI-6922762 ba_f3 Mouse pro B cells IL-3 dependent BA/F3 baf3 Mouse pro B cell line. IL-3 dependent murine pro B cell line established from peripheral blood; apparently derived from BALB/c mouse. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiao W., Hong H., Kawakami Y., Kato Y., Wu D., Yasudo H., Kimura A., Kubagawa H., Bertoli LF., Davis RS., Chau LA., Madrenas J., Hsia CC., Xenocostas A., Kipps TJ., Hennighausen L., Iwama A., Nakauchi H., Kawakami T. Cancer - Interactions investigated in the context of cancer 10 ng/ml of IL-3 was added for 0-10 minutes. STAT5A has been entered throuhout, as no specific info is given in this paper as to whether STAT5A or STAT5B was used. toshi@liai.org Cancer Cell (1535-6108) 2009 Only protein-protein interactions 4 imex curation xiao-2009-2 Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. mouse-spleen Mus musculus spleen spleen spleen anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiao W., Hong H., Kawakami Y., Kato Y., Wu D., Yasudo H., Kimura A., Kubagawa H., Bertoli LF., Davis RS., Chau LA., Madrenas J., Hsia CC., Xenocostas A., Kipps TJ., Hennighausen L., Iwama A., Nakauchi H., Kawakami T. Cancer - Interactions investigated in the context of cancer 10 ng/ml of IL-3 was added for 0-10 minutes. STAT5A has been entered throuhout, as no specific info is given in this paper as to whether STAT5A or STAT5B was used. toshi@liai.org IL3 (ng/ml) Time (mins) Cancer Cell (1535-6108) 2009 Only protein-protein interactions 4 imex curation plcb3_mouse 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Plcb3 Phosphoinositide phospholipase C-beta-3 Phospholipase C-beta-3 protein protein mouse Mus musculus Mouse MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQSNSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEEEDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHKKHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQADVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVELTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRCLLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL ptn6_mouse Tyrosine-protein phosphatase non-receptor type 6 Ptpn6 Hcp Hcph Ptp1C Protein-tyrosine phosphatase 1C Hematopoietic cell protein-tyrosine phosphatase 70Z-SHP SH-PTP1 PTPTY-42 protein protein mouse Mus musculus Mouse MVRWFHRDLSGPDAETLLKGRGVPGSFLARPSRKNQGDFSLSVRVDDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGILQDRDGTIIHLKYPLNCSDPTSERWYHGHISGGQAESLLQAKGEPWTFLVRESLSQPGDFVLSVLNDQPKAGPGSPLRVTHIKVMCEGGRYTVGGSETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKSKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYVKNQLLGPDENSKTYIASQGCLDATVNDFWQMAWQENTRVIVMTTREVEKGRNKCVPYWPEVGTQRVYGLYSVTNSREHDTAEYKLRTLQISPLDNGDLVREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMESISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEIIQSQKGQESEYGNITYPPAVRSAHAKASRTSSKHKEEVYENVHSKSKKEEKVKKQRSADKEKNKGSLKRK pic3b-stat5-ptn6 884757 n/a 884759 unspecified role unspecified role bait bait n/a 884760 unspecified role unspecified role prey prey association association false false false Pairwise interactions among PLC-b3, Ptpn6/SHP-1, and Stat5 were modestly induced upon IL-3 stimulation in nontransduced Ba/F cells and splenocytes from normal mice. STAT5A/B was also present, but it is not known which one it was. Fig 4D. Immunodepletion of STAT5 showed this molecule to be required for the interaction between Ptpn6 and Plcb3 to occur. ptn6-stat5-picb3 884757 n/a 884759 unspecified role unspecified role prey prey n/a 884760 unspecified role unspecified role bait bait association association false false false Pairwise interactions among PLC-B3, Ptpn6/SHP-1, and Stat5 were modestly induced upon IL-3 stimulation in nontransduced Ba/F cells and splenocytes from normal mice. STAT5A/B was also present, but it is not known which one it was. Fig 4D. Sup. Fig. 11B Immunodepletion of STAT5 showed this molecule to be required for the interaction between Ptpn6 and Plcb3 to occur. pic3b-ptn6-1 884758 n/a 884759 unspecified role unspecified role bait bait n/a 884760 unspecified role unspecified role prey prey physical association physical association false false false In WT mice, Ptpn6/SHP-1 was detected using PLC-B3 for IP. An 80% reduction in Stat5 expression did not affect the PLC-B3/SHP-1 interaction. Fig 4E. 0 0 picb3-stat5-ptn6 884758 n/a 884759 unspecified role unspecified role bait bait n/a 884760 unspecified role unspecified role prey prey physical association physical association false false false IL-3-inducible interactions between PLC-B3 and Stat5 were also shown in mouse splenocytes. STAT5A/B was present, but it is not known which one it was. Fig S9A. 10 0-3