IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ carreira-2009-2 The BRC repeats of BRCA2 modulate the DNA-binding selectivity of RAD51. in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Carreira A., Hilario J., Amitani I., Baskin RJ., Shivji MK., Venkitaraman AR., Kowalczykowski SC. Cancer - Interactions investigated in the context of cancer sckowalczykowski@ucdavis.edu Cell (0092-8674) 2009 Only protein-protein interactions imex curation carreira-2009-1 The BRC repeats of BRCA2 modulate the DNA-binding selectivity of RAD51. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Carreira A., Hilario J., Amitani I., Baskin RJ., Shivji MK., Venkitaraman AR., Kowalczykowski SC. Cancer - Interactions investigated in the context of cancer sckowalczykowski@ucdavis.edu Cell (0092-8674) 2009 Only protein-protein interactions imex curation atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rad51_human DNA repair protein RAD51 homolog 1 RAD51 RAD51A RECA RAD51 homolog A protein protein human Homo sapiens Human MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD dt40_dna 40-mer poly-dT (dT40) ss dna single stranded deoxyribonucleic acid ss DNA ss dna chemical synthesis Chemical synthesis (Chemical synthesis) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT dt60_dna 60-mer poly-dT (dT60) ss dna single stranded deoxyribonucleic acid ss DNA ss dna chemical synthesis Chemical synthesis (Chemical synthesis) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT dt90_dna 90-mer poly-dT (dT90) ss dna single stranded deoxyribonucleic acid ss DNA ss dna chemical synthesis Chemical synthesis (Chemical synthesis) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT brca2_human Breast cancer type 2 susceptibility protein BRCA2 FANCD1 FACD Fanconi anemia group D1 protein protein protein human Homo sapiens Human MPIGSKERPTFFEIFKTRCNKADLGPISLNWFEELSSEAPPYNSEPAEESEHKNNNYEPNLFKTPQRKPSYNQLASTPIIFKEQGLTLPLYQSPVKELDKFKLDLGRNVPNSRHKSLRTVKTKMDQADDVSCPLLNSCLSESPVVLQCTHVTPQRDKSVVCGSLFHTPKFVKGRQTPKHISESLGAEVDPDMSWSSSLATPPTLSSTVLIVRNEEASETVFPHDTTANVKSYFSNHDESLKKNDRFIASVTDSENTNQREAASHGFGKTSGNSFKVNSCKDHIGKSMPNVLEDEVYETVVDTSEEDSFSLCFSKCRTKNLQKVRTSKTRKKIFHEANADECEKSKNQVKEKYSFVSEVEPNDTDPLDSNVANQKPFESGSDKISKEVVPSLACEWSQLTLSGLNGAQMEKIPLLHISSCDQNISEKDLLDTENKRKKDFLTSENSLPRISSLPKSEKPLNEETVVNKRDEEQHLESHTDCILAVKQAISGTSPVASSFQGIKKSIFRIRESPKETFNASFSGHMTDPNFKKETEASESGLEIHTVCSQKEDSLCPNLIDNGSWPATTTQNSVALKNAGLISTLKKKTNKFIYAIHDETSYKGKKIPKDQKSELINCSAQFEANAFEAPLTFANADSGLLHSSVKRSCSQNDSEEPTLSLTSSFGTILRKCSRNETCSNNTVISQDLDYKEAKCNKEKLQLFITPEADSLSCLQEGQCENDPKSKKVSDIKEEVLAAACHPVQHSKVEYSDTDFQSQKSLLYDHENASTLILTPTSKDVLSNLVMISRGKESYKMSDKLKGNNYESDVELTKNIPMEKNQDVCALNENYKNVELLPPEKYMRVASPSRKVQFNQNTNLRVIQKNQEETTSISKITVNPDSEELFSDNENNFVFQVANERNNLALGNTKELHETDLTCVNEPIFKNSTMVLYGDTGDKQATQVSIKKDLVYVLAEENKNSVKQHIKMTLGQDLKSDISLNIDKIPEKNNDYMNKWAGLLGPISNHSFGGSFRTASNKEIKLSEHNIKKSKMFFKDIEEQYPTSLACVEIVNTLALDNQKKLSKPQSINTVSAHLQSSVVVSDCKNSHITPQMLFSKQDFNSNHNLTPSQKAEITELSTILEESGSQFEFTQFRKPSYILQKSTFEVPENQMTILKTTSEECRDADLHVIMNAPSIGQVDSSKQFEGTVEIKRKFAGLLKNDCNKSASGYLTDENEVGFRGFYSAHGTKLNVSTEALQKAVKLFSDIENISEETSAEVHPISLSSSKCHDSVVSMFKIENHNDKTVSEKNNKCQLILQNNIEMTTGTFVEEITENYKRNTENEDNKYTAASRNSHNLEFDGSDSSKNDTVCIHKDETDLLFTDQHNICLKLSGQFMKEGNTQIKEDLSDLTFLEVAKAQEACHGNTSNKEQLTATKTEQNIKDFETSDTFFQTASGKNISVAKESFNKIVNFFDQKPEELHNFSLNSELHSDIRKNKMDILSYEETDIVKHKILKESVPVGTGNQLVTFQGQPERDEKIKEPTLLGFHTASGKKVKIAKESLDKVKNLFDEKEQGTSEITSFSHQWAKTLKYREACKDLELACETIEITAAPKCKEMQNSLNNDKNLVSIETVVPPKLLSDNLCRQTENLKTSKSIFLKVKVHENVEKETAKSPATCYTNQSPYSVIENSALAFYTSCSRKTSVSQTSLLEAKKWLREGIFDGQPERINTADYVGNYLYENNSNSTIAENDKNHLSEKQDTYLSNSSMSNSYSYHSDEVYNDSGYLSKNKLDSGIEPVLKNVEDQKNTSFSKVISNVKDANAYPQTVNEDICVEELVTSSSPCKNKNAAIKLSISNSNNFEVGPPAFRIASGKIVCVSHETIKKVKDIFTDSFSKVIKENNENKSKICQTKIMAGCYEALDDSEDILHNSLDNDECSTHSHKVFADIQSEEILQHNQNMSGLEKVSKISPCDVSLETSDICKCSIGKLHKSVSSANTCGIFSTASGKSVQVSDASLQNARQVFSEIEDSTKQVFSKVLFKSNEHSDQLTREENTAIRTPEHLISQKGFSYNVVNSSAFSGFSTASGKQVSILESSLHKVKGVLEEFDLIRTEHSLHYSPTSRQNVSKILPRVDKRNPEHCVNSEMEKTCSKEFKLSNNLNVEGGSSENNHSIKVSPYLSQFQQDKQQLVLGTKVSLVENIHVLGKEQASPKNVKMEIGKTETFSDVPVKTNIEVCSTYSKDSENYFETEAVEIAKAFMEDDELTDSKLPSHATHSLFTCPENEEMVLSNSRIGKRRGEPLILVGEPSIKRNLLNEFDRIIENQEKSLKASKSTPDGTIKDRRLFMHHVSLEPITCVPFRTTKERQEIQNPNFTAPGQEFLSKSHLYEHLTLEKSSSNLAVSGHPFYQVSATRNEKMRHLITTGRPTKVFVPPFKTKSHFHRVEQCVRNINLEENRQKQNIDGHGSDDSKNKINDNEIHQFNKNNSNQAAAVTFTKCEEEPLDLITSLQNARDIQDMRIKKKQRQRVFPQPGSLYLAKTSTLPRISLKAAVGGQVPSACSHKQLYTYGVSKHCIKINSKNAESFQFHTEDYFGKESLWTGKGIQLADGGWLIPSNDGKAGKEEFYRALCDTPGVDPKLISRIWVYNHYRWIIWKLAAMECAFPKEFANRCLSPERVLLQLKYRYDTEIDRSRRSAIKKIMERDDTAAKTLVLCVSDIISLSANISETSSNKTSSADTQKVAIIELTDGWYAVKAQLDPPLLAVLKNGRLTVGQKIILHGAELVGSPDACTPLEAPESLMLKISANSTRPARWYTKLGFFPDPRPFPLPLSSLFSDGGNVGCVDVIIQRAYPIQWMEKTSSGLYIFRNEREEEKEAAKYVEAQQKRLEALFTKIQEEFEEHEENTTKPYLPSRALTRQQVRALQDGAELYEAVKNAADPAYLEGYFSEEQLRALNNHRQMLNDKKQAQIQLEIRKAMESAEQKEQGLSRDVTTVWKLRIVSYSKKEKDSVILSIWRPSSDLYSLLTEGKRYRIYHLATSKSKSKSERANIQLAATKKTQYQQLPVSDEILFQIYQPREPLHFSKFLDPDFQPSCSEVDLIGFVVSVVKKTGLAPFVYLSDECYNLLAIKFWIDLNEDIIKPHMLIAASNLQWRPESKSGLLTLFAGDFSVFSASPKEGHFQETFNKMKNTVENIDILCNEAENKLMHILHANDPKWSTPTKDCTSGPYTAQIIPGTGNKLLMSSPNCEIYYQSPLSLCMAKRKSVSTPVSAQMTSKSCKGEKEIDDQKNCKKRRALDFLSRLPLPPPVSPICTFVSPAAQKAFQPPRSCGTKYETPIKKKELNSPQMTPFKKFNEISLLESNSIADEELALINTQALLSGSTGEKQFISVSESTRTAPTSSEDYLRLKRRCTTSLIKEQESSQASTEECEKNKQDTITTKKYI m13 Phage M13 genome dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid bpm13 Enterobacteria phage M13 Bacteriophage M13 phix174_ssdna Enterobacteria phage phiX174 genome ss dna single stranded deoxyribonucleic acid ss DNA ss dna bpphx Enterobacteria phage phiX174 Bacteriophage phi-X174 phix174_dsdna Enterobacteria phage phiX174 linear dsDNA ds dna double stranded deoxyribonucleic acid ds DNA ds dna bpphx Enterobacteria phage phiX174 Bacteriophage phi-X174 rad51-atp-1 ATP hydrolysis by RAD51 803988 n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803992 unspecified role unspecified role ancillary ancillary atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-2 ATP hydrolysis by RAD51 803988 n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803993 unspecified role unspecified role ancillary ancillary atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-3 ATP hydrolysis by RAD51 803988 n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component n/a 803994 unspecified role unspecified role ancillary ancillary atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-4 ATP hydrolysis by RAD51 - inhibition by BRCA2 803988 n/a 803995 inhibitor inhibitor neutral component neutral component brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 803992 unspecified role unspecified role ancillary ancillary n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-5 ATP hydrolysis by RAD51 - inhibition by BRCA2 803988 n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component n/a 803995 inhibitor inhibitor neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803993 unspecified role unspecified role ancillary ancillary atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-6 ATP hydrolysis by RAD51 - inhibition by BRCA2 803988 n/a 803995 inhibitor inhibitor neutral component neutral component brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 803994 unspecified role unspecified role ancillary ancillary n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-7 ATP hydrolysis by RAD51 - inhibition by BRCA2 803988 n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component n/a 803995 inhibitor inhibitor neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 803996 unspecified role unspecified role ancillary ancillary atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-8 ATP hydrolysis by RAD51 803988 n/a 803996 unspecified role unspecified role ancillary ancillary n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-9 ATP hydrolysis by RAD51 - inhibition by BRCA2 803988 n/a 803997 unspecified role unspecified role ancillary ancillary n/a 803995 inhibitor inhibitor neutral component neutral component brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-atp-10 ATP hydrolysis by RAD51 803988 n/a 803997 unspecified role unspecified role ancillary ancillary n/a 803991 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) n/a 803990 enzyme target enzyme target substrate substrate neutral component neutral component atpase reaction atpase reaction false false false Fig. 2A ATP is 32P labelled. rad51-dt40-1 DNA binding of RAD51 803989 n/a 803992 unspecified role unspecified role prey prey n/a 803991 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1A dT40 is 32P labelled. rad51-dt40-2 Enhancement of ss-DNA binding of RAD51 by BRCA2 803989 n/a 803995 stimulator stimulator ancillary ancillary P51587:p.Thr1526Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 803991 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 803992 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1 dT40 is 32P labelled. Interaction required Mg2+ and ATP. In the absence of ATP, BRCA2 inhibited filament formation. rad51-phix174-1 DNA binding of RAD51 803989 n/a 803991 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 803998 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig. 3A phiX174 is 32P labelled. rad51-phix174-2 Inhibition of ds-DNA binding of RAD51 by BRCA2 803989 n/a 803991 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 803995 inhibitor inhibitor ancillary ancillary P51587:p.Thr1526Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 803998 unspecified role unspecified role prey prey physical association physical association false false false Figs. 3A, 3B, Supp. Fig. 4B phiX174 is 32P labelled. Interaction required ATP. rad51-dt40-3 Enhancement of ss-DNA binding of RAD51 by BRCA2 803989 n/a 803992 unspecified role unspecified role prey prey n/a 803991 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) n/a 803995 stimulator stimulator ancillary ancillary brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Supp. Fig. 2 dT40 is 32P labelled. rad51-phix174-3 Inhibition of ds-DNA binding of RAD51 by BRCA2 803989 n/a 803998 unspecified role unspecified role prey prey n/a 803995 inhibitor inhibitor ancillary ancillary brc4 repeat region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 803991 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association false false false Supp. Fig. 4 phiX174 is 32P labelled. Interaction required ATP.