IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ enge-2009-2 MDM2-dependent downregulation of p21 and hnRNP K provides a switch between apoptosis and growth arrest induced by pharmacologically activated p53. human Homo sapiens Human ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Enge M., Bao W., Hedstrom E., Jackson SP., Moumen A., Selivanova G. Apoptosis - Interactions involving proteins with a function related to apoptosis There is no statement regarding the cell line used for chIP. galina.selivanova@ki.se Cancer Cell (1535-6108) 2009 Only protein-protein interactions 1 imex curation Cancer - Interactions investigated in the context of cancer enge-2009-1 MDM2-dependent downregulation of p21 and hnRNP K provides a switch between apoptosis and growth arrest induced by pharmacologically activated p53. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Enge M., Bao W., Hedstrom E., Jackson SP., Moumen A., Selivanova G. Apoptosis - Interactions involving proteins with a function related to apoptosis galina.selivanova@ki.se Cancer Cell (1535-6108) 2009 Only protein-protein interactions 1 imex curation Cancer - Interactions investigated in the context of cancer cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD hnrpk_human Heterogeneous nuclear ribonucleoprotein K HNRNPK HNRPK Transformation up-regulated nuclear protein protein protein human Homo sapiens Human METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP p53-cdkn1a-1 874994 n/a 874996 unspecified role unspecified role prey prey n/a 874997 unspecified role unspecified role bait bait association association false false false Fig 5C. It was found that the binding of p53 to the CDKN1A promoter is induced equally upon RITA and nutlin3a treatment. RITA (a low-molecular-weight p53-reactivating compound), nutlin3a (another low-molecular-weight compound that activates p53). p53 bound to a 3' region of the CDKN1A (p21)promoter. p53-cdkn1a 874994 n/a 874996 unspecified role unspecified role prey prey n/a 874997 unspecified role unspecified role bait bait association association false false false Fig 5C. It was found that the binding of p53 to the CDKN1A promoter is induced equally upon RITA and nutlin3a treatment. RITA (a low-molecular-weight p53-reactivating compound), nutlin3a (another low-molecular-weight compound that activates p53). p53 bound to a 5' region of the CDKN1A (p21)promoter. hnrpk-cdkn1a 874994 n/a 874996 unspecified role unspecified role prey prey n/a 874998 unspecified role unspecified role bait bait association association false false false Fig 5C. It was observed that nutlin3a caused a significant induction of hnRNP K binding to the upstream site of the CDKN1A promoter. nutlin3a, (another low-molecular-weight compound that activates p53). Heterogeneous nuclear ribonucleoprotein K bound to a 5' region of the CDKN1A (p21)promoter. mdm2-hnrnpk 874995 n/a 874999 unspecified role unspecified role prey prey n/a 874998 unspecified role unspecified role bait bait physical association physical association false false false Fig 4F. An increased binding of MDM2 to hnRNP K upon RITA treatment was observed, in contrast to other treatments. RITA - a low-molecular-weight p53-reactivating compound.