IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhang-2008-4 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer zhang-2008-6 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu Paclitaxel treatment or no treatment J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer zhang-2008-1 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer zhang-2008-2 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer zhang-2008-3 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer zhang-2008-5 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer zhang-2008-7 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Coimmunoprecipitation was carried out upon the glycerol gradient fractions. lu-hai.wang@mssm.edu J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer zhang-2008-8 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Coimmunoprecipitation was carried out upon the glycerol gradient fractions. lu-hai.wang@mssm.edu J. Biol. Chem. (0021-9258) 2008 Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer dyl1_human Dynein light chain 1, cytoplasmic DYNLL1 DLC1 DNCLC1 HDLC1 Dynein light chain LC8-type 1 8 kDa dynein light chain Protein inhibitor of neuronal nitric oxide synthase DNCL1 protein protein human Homo sapiens Human MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR o54918-1 Bcl-2-like protein 11 Bcl2l11 Bim Bcl2-interacting mediator of cell death protein protein mouse Mus musculus Mouse MAKQPSDVSSECDREGGQLQPAERPPQLRPGAPTSLQTEPQGNPDGEGDRCPHGSPQGPLAPPASPGPFATRSPLFIFVRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASIRQSQEEPEDLRPEIRIAQELRRIGDEFNETYTRRVFANDYREAEDHPQMVILQLLRFIFRLVWRRH o43521-1 Bcl-2-like protein 11 Bim(EL) BCL2L11 BIM Bcl2-interacting mediator of cell death protein protein human Homo sapiens Human MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH tom20_human Mitochondrial import receptor subunit TOM20 homolog Outer mitochondrial membrane receptor Tom20 TOMM20 KIAA0016 Mitochondrial 20 kDa outer membrane protein protein protein human Homo sapiens Human MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE b2l11_human Bcl-2-like protein 11 BCL2L11 BIM Bcl2-interacting mediator of cell death protein protein human Homo sapiens Human MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH cish_human Cytokine-inducible SH2-containing protein CIS-1 Suppressor of cytokine signaling Protein G18 CISH G18 protein protein human Homo sapiens Human MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL dynll1-gnb2l1-1 GNB2L1 (RACK1) pulls down DYNLL1 (DLC1) in vitro 733933 N/A 733941 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 733942 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region WD1-4 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1 D The range for the WD repeats 1-4 for GNB2L1 (RACK1) was taken from the UniProtKB entry for the protein. The peptide pWD4 based on aa 138-146 of GNB2L1 (RACK1) (CKYTVQDES) inhibits this interaction. bcl2l11-gnb2l1 BCL2L11 (BimEL) pulls down GNB2L1 (RACK1) in vitro 733933 N/A 733942 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) N/A 733943 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1 E This interaction was enhanced by the presence DYNLL1 (DLC1). bcl2l11-dynll1 DYNLL1 (DLC1), BCL2L11 and GNB2L1 distribute to the same sedimentation fractions 733934 N/A 733944 unspecified role unspecified role neutral component neutral component N/A 733941 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 733942 unspecified role unspecified role neutral component neutral component physical association physical association false false false S2 A No paclitaxel treatment bcl2l11-dynll1-1 DYNLL1 (DLC1), BCL2L11 and GNB2L1 distribute to the same sedimentation fractions 733934 N/A 733942 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 733945 unspecified role unspecified role neutral component neutral component N/A 733941 unspecified role unspecified role neutral component neutral component N/A 733944 unspecified role unspecified role neutral component neutral component physical association physical association false false false S2 A Paclitaxel treatment Paclitaxel induced release of BCL2L11 (BimEL) and DYNLL1 (DLC1) from microtubule. dynll1-gnb2l1 DYNLL1 (DLC1) coimmunoprecipitates GNB2L1 (RACK1) in HEK293T lysates 733935 N/A 733941 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 733942 unspecified role unspecified role prey prey physical association physical association false false false P63167 transfection 1 A UV-irradiation - This interaction occurred upon treatment of cells with UV-irradiation. Paclitaxel - This interaction occurred upon treatment of cells with paclitaxel. Staurosporine - This interaction occurred upon treatment of cells with staurosporine. bcl2l2l11-gnb2l1 BCL2L11 (BIM) coimmunoprecipitates GNB2L1 (RACK1) in MCF7 lysates 733936 N/A 733946 unspecified role unspecified role bait bait N/A 733942 unspecified role unspecified role prey prey physical association physical association false false false 1 B Paclitaxel - This interaction occurred upon treatment of cells with paclitaxel. Staurosporine - This interaction occurred upon treatment of cells with staurosporine. dynll1-bcl2l11 DYNLL1 (DLC1) coimmunoprecipitates GNB2L1 (RACK1), CISH (CIS) and BCL2L11 (BIM) in HEK293T lysates 733937 N/A 733941 unspecified role unspecified role bait bait N/A 733944 unspecified role unspecified role prey prey N/A 733947 unspecified role unspecified role prey prey N/A 733942 unspecified role unspecified role prey prey association association false false false 1 C Paclitaxel - These interactions were increased by treating cell with paclitaxel. bcl2l11-gnb2l1-1 BCL2L11 (BimEL) coimmunoprecipitates GNB2L1 (RACK1) in HEK293T lysates 733938 N/A 733943 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 733942 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region WD1-4 sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false S1 O54918-1; P63244 transfection The range for the WD repeats 1-4 was taken from the UniProtKB entry for GNB2L1 (RACK1), P63244. Paclitaxel treatment of cells decreased this interaction. bcl2l11-gnb2l1-2 BCL2L11 (BIM) coimmunoprecipitates GNB2L1 (RACK1) in MCF7 sedimentation fractions 733939 n/a 733946 unspecified role unspecified role bait bait N/A 733942 unspecified role unspecified role prey prey physical association physical association false false false S2 B This interaction was enhanced when cells were treated with paclitaxel. dynll1-gnb2l1-2 DYNll1 (DLC1) coimmunoprecipitates GNB2L1 (RACK1) from HEK293T glycerol gradient fractions 733940 N/A 733942 unspecified role unspecified role prey prey N/A 733941 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S2 C Paclitaxel treatment of cells enhanced this interaction.