IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nakatsuji-2008-3 Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation nakatsuji-2008-1 Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation nakatsuji-2008-2 Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation nakatsuji-2008-4 Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. mouse-mtd_1a Mus musculus simple epithelial cell line MTD-1A mtd_1a Mouse simple epithelial cell line MTD-1A anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation nakatsuji-2008-5 Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. mouse-mtd_1a Mus musculus simple epithelial cell line MTD-1A mtd_1a Mouse simple epithelial cell line MTD-1A confocal microscopy confocal microscopy immunostaining immunostaining Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 Only protein-protein interactions imex curation milk2_mouse MICAL-like protein 2 Micall2 Jrab Junctional Rab13-binding protein Molecule interacting with CasL-like 2 protein protein mouse Mus musculus Mouse MAAIKALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHRPDLINFSALRKENIYENNKLAFQVAEEQLGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTVQEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVTTTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGVKTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPGPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQEEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA actn4_mouse Alpha-actinin-4 Non-muscle alpha-actinin 4 Actn4 protein protein mouse Mus musculus Mouse MVDYHAANQAYQYGPNSGGGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKQRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPSLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL actb_mouse Actin, cytoplasmic 1 Actb Beta-actin protein protein mouse Mus musculus Mouse MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF rab13_human Ras-related protein Rab-13 RAB13 GIG4 Cell growth-inhibiting gene 4 protein protein protein human Homo sapiens Human MAKAYDHLFKLLLIGDSGVGKTCLIIRFAEDNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKCSLG micall2-actn4-2 JRAB/MICAL-L2 coimmunoprecipitates Alpha-actinin-4 in BHK lysates 740153 N/A 740158 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740159 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false BAIT gene AB182579; PREY gene NM_021895 2 B, 3 B P57780; Q3TN34 transfection actn4-micall2 JRAB/MICAL-L2 coimmunoprecipitates with Alpha-actinin-4 in BHK lysates 740153 N/A 740159 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740158 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false BAIT gene NM_021895; PREY gene AB182579 2 C P57780; Q3TN34 transfection micall2-actn4-4 JRAB/MICAL-L2 coimmunoprecipitates Alpha-actinin-4 and beta-actin in BHK lysates 740153 N/A 740160 unspecified role unspecified role prey prey N/A 740158 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740159 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false BAIT gene AB182579; PREY gene NM_021895 3 C P57780; Q3TN34 transfection rab13-actn4 RAB13 coimmunoprecipitates Alpha-actinin-4 in BHK lysates 740153 N/A 740158 stimulator stimulator prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740161 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740159 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false PREY gene NM_021895 4 A Q3TN34; P57780; P51153 transfection This interaction occurred in the presence of Myc-JRAB/MICAL-L2. micall2-actn4-5 JRAB/MICAL-L2 coimmunoprecipitates Alpha-actinin-4 and Rab13 in BHK lysates 740153 N/A 740158 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740161 unspecified role unspecified role prey prey P51153:p.Thr22Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P51153:p.Gln67Leu mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740159 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false BAIT gene AB182579; PREY gene NM_021895 4 B, D P57780; Q3TN34; P51153 transfection The amount of Myc-actinin-4-F coimmunoprecipitated with HA-JRAB/MICAL-L2 was increased in the presence of Myc-Rab13 Q67L compared to that of Myc-Rab13 T22N. From Fig. 4 B it is not clear if any Rab13 was coimmunoprecipitated when the mutant Rab13 T22N was used. Rab13 T22N is a GTP binding mutant and Rab13 Q67L is a GTP hydrolysis mutant. Th presence of wild-type Rab13 and GTPgammaS increased these interactions in a dose-dependent manner. actn4-micall2-2 JRAB/MICAL-L2 and Rab13 coimmunoprecipitate with Alpha-actinin-4 in BHK lysates 740153 N/A 740161 unspecified role unspecified role prey prey P51153:p.Thr22Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P51153:p.Gln67Leu mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740158 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 740159 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false PREY gene AB182579; BAIT gene NM_021895 4 C P57780; Q3TN34; P51153 transfection The amount of Myc-actinin-4-F coimmunoprecipitated with HA-JRAB/MICAL-L2 was increased in the presence of Myc-Rab13 Q67L compared to that of Myc-Rab13 T22N. Rab13 T22N is a GTP binding mutant and Rab13 Q67L is a GTP hydrolysis mutant. micall2-actn4 JRAB/MICAL-L2 interacts with Alpha-actinin-4 in a yeast two-hybrid assay 740154 N/A 740159 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 740158 unspecified role unspecified role bait bait region MN sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false BAIT gene AB182579; PREY gene NM_021895 micall2-actn4-1 JRAB/MICAL-L2 interacts with Alpha-actinin-4 in a directed yeast two-hybrid assay 740155 N/A 740158 unspecified role unspecified role bait bait region MN sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 740159 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false BAIT gene AB182579; PREY gene NM_021895 2 A micall2-actn4-3 JRAB/MICAL-L2 coimmunoprecipitates Alpha-actinin-4 in MTD-1A lysates 740156 N/A 740158 unspecified role unspecified role bait bait N/A 740159 unspecified role unspecified role prey prey physical association physical association false false false BAIT gene AB182579; PREY gene NM_021895 2 D actn4-micall2-1 JRAB/MICAL-L2 and Alpha-actinin-4 colocalize in MTD-1A cells 740157 N/A 740158 unspecified role unspecified role neutral component neutral component N/A 740159 unspecified role unspecified role neutral component neutral component physical association physical association false false false Q3TN34 gene AB182579; P57780 gene NM_021895 2 E This colocalization took place primarily at cell-cell junctions.