IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mantovani-2007-1 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation mantovani-2007-9 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation mantovani-2007-2 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer Details for Pull Down method obtained from Shen et al,. Genes + Dev. 1998. GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation mantovani-2007-3 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation mantovani-2007-4 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation mantovani-2007-6 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation mantovani-2007-8 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation mantovani-2007-10 The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mantovani F., Tocco F., Girardini J., Smith P., Gasco M., Lu X., Crook T., Del Sal G. Cancer - Interactions investigated in the context of cancer GST-Pin1 and related constructs have been entered as Human due to mutation locations. However recombinant p300 and the GFP-p300 construct cannot be entered as the authors have not confirmed their origin. (Figs 4A , S8A and 4E) delsal@lncib.trieste.it Nat. Struct. Mol. Biol. (1545-9993) 2007 Only protein-protein interactions imex curation cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human pin1_human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 PIN1 protein protein human Homo sapiens Human MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD bax_human_gene Human BAX gene gene gene human Homo sapiens Human ep300_human Histone acetyltransferase p300 Histone butyryltransferase p300 Histone crotonyltransferase p300 Protein propionyltransferase p300 EP300 P300 E1A-associated protein p300 Protein 2-hydroxyisobutyryltransferase p300 Protein lactyltransferas p300 protein protein human Homo sapiens Human MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEPLQQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLSNNLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQIDPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVNGGVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMIRGSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGPRMQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQGSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPPGPQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAPLLPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDDESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLRRRMASMQRTGVVGQQQGLPSPTPATPTTPTGQQPTTPQTPQPTSQPQPTPPNSMPPYLPRTQAAGPVSQGKAAGQVTPPTPPQTAQPPLPGPPPAAVEMAMQIQRAAETQRQMAHVQIFQRPIQHQMPPMTPMAPMGMNPPPMTRGPSGHLEPGMGPTGMQQQPPWSQGGLPQPQQLQSGMPRPAMMSVAQHGQPLNMAPQPGLGQVGISPLKPGTVSQQALQNLLRTLRSPSSPLQQQQVLSILHANPQLLAAFIKQRAAKYANSNPQPIPGQPGMPQGQPGLQPPTMPGQQGVHSNPAMQNMNPMQAGVQRAGLPQQQPQQQLQPPMGGMSPQAQQMNMNHNTMPSQFRDILRRQQMMQQQQQQGAGPGIGPGMANHNQFQQPQGVGYPPQQQQRMQHHMQQMQQGNMGQIGQLPQALGAEAGASLQAYQQRLLQQQMGSPVQPNPMSPQQHMLPNQAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPSPRMQPQPSPHHVSPQTSSPHPGLVAAQANPMEQGHFASPDQNSMLSQLASNPGMANLHGASATDLGLSTDNSDLNSNLSQSTLDIH iaspp_human RelA-associated inhibitor PPP1R13L IASPP NKIP1 PPP1R13BL RAI Inhibitor of ASPP protein NFkB-interacting protein 1 PPP1R13B-like protein protein protein human Homo sapiens Human MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQPQPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV probe_p21_(cdkn1a)_p53_dna_binding_site CDKN1A_ p53 binding site dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) AGCTTAGGCATGTCTAGGCATGTCTA Blatting the stated sequence does not give a "hit" to any genomic region. tp53-cdkn1a-9 1288042 n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288051 unspecified role unspecified role ancillary ancillary n/a 1288052 unspecified role unspecified role bait bait association association false false false Fig IB, 1C, Fig 2. UV light, etoposide, Nutlin-3a (inhibits Mdm2 binding to p53). Inhibition of Pin1 expression in stressed cells, reduced the binding of p53 to its upstream responsive site (BS1) on the p21 promoter. tp53-bax-1 1288042 n/a 1288052 unspecified role unspecified role bait bait n/a 1288053 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288051 unspecified role unspecified role ancillary ancillary association association false false false Fig IC, fig 2. UV light, etoposide, Nutlin-3A (inhibits Mdm2 binding to p53). RNAi inhibition of Pin1 expression in stressed cells, and stabilizing p53 with nutlin-3a, show that Pin1 is required for optimal binding of p53 to both p21 and BAX promoters. pin1-cdkn1a 1288042 n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288051 unspecified role unspecified role bait bait association association false false false Fig 2, S1B. etoposide. DNA damage–induced recruitment of Pin1 is only slightly evident in HCT116 p53-/- cells. (Fig S1B) pin1-bax-1 1288042 n/a 1288053 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288051 unspecified role unspecified role bait bait association association false false false Fig 2, S1B. etoposide. DNA damage–induced recruitment of Pin1 is only slightly evident in HCT116 p53-/- cells. (Fig S1B). tp53-cdkn1a-12 1288042 n/a 1288052 unspecified role unspecified role bait bait ac-lys373/382 n6aclys N6-acetyl-L-lysine (2S)-6-acetamido-2-aminohexanoic acid 6-acetylamino-2-aminohexanoic acid 6-acetylazanyl-2-aminohexanoic acid MOD_RES N6-acetyllysine N(zeta)-acetyllysine N6-acetyl-L-lysine N6-acetylated L-lysine epsilon-acetyllysine Acetyl Acetylation N6AcLys acetylk certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5C Acetylation of promoter-bound p53 on Lys373 and Lys382 increased upon stress only in the presence of normal levels of Pin1, and could be blocked by inhibition of Pin1's catalytic activity with the small molecule PiB. PiB. ep300-cdkn1a-1 1288042 n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288054 unspecified role unspecified role bait bait association association false false false 5D RNAi for Pin1. tp53-cdkn1a-14 1288043 n/a 1288050 unspecified role unspecified role prey prey bs1_upstream_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288052 unspecified role unspecified role bait bait association association false false false Fig S5B etoposide. pin1-cdkn1a-4 1288043 n/a 1288051 unspecified role unspecified role bait bait n/a 1288050 unspecified role unspecified role prey prey bs1+2_promoter_regions binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S1A. UV light, etoposide. tp53-cdkn1a-15 1288043 n/a 1288052 unspecified role unspecified role bait bait n/a 1288050 unspecified role unspecified role prey prey bs1+2_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig S1A UV light, etoposide. tp53-cdkn1a-16 1288043 n/a 1288052 unspecified role unspecified role bait bait ac-lys382 n6aclys N6-acetyl-L-lysine (2S)-6-acetamido-2-aminohexanoic acid 6-acetylamino-2-aminohexanoic acid 6-acetylazanyl-2-aminohexanoic acid MOD_RES N6-acetyllysine N(zeta)-acetyllysine N6-acetyl-L-lysine N6-acetylated L-lysine epsilon-acetyllysine Acetyl Acetylation N6AcLys acetylk certain certain sequence position certain certain certain sequence position certain false n/a 1288050 unspecified role unspecified role prey prey bs1_upstream_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig S5B etoposide. ep300-cdkn1a 1288043 n/a 1288054 unspecified role unspecified role bait bait n/a 1288050 unspecified role unspecified role prey prey bs1_upstream_promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig S5B etoposide. Binding was reduced when Pin1 was knocked down. pin1-tp53-14 1288044 n/a 1288051 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1288052 unspecified role unspecified role prey prey P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma physical association physical association false false false Fig 3A pin1-tp53-15 1288044 n/a 1288052 unspecified role unspecified role prey prey P04637:p.Pro72Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. n/a 1288051 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig S2B, S5A, S6C. lambda phosphatase Binding was observed using mutant p53 proteins with only one intact Pin1-consensus site. p53-9KR, (a mutant where nine C-terminal lysines, including those acetylated by p300, are substituted by arginines), bound Pin1 to a similar extent as did WT p53. pin1-cdkn1a-3 1288045 n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288052 unspecified role unspecified role ancillary ancillary P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1288051 unspecified role unspecified role bait bait association association false false false Fig 3B tp53-cdkn1a-11 1288045 n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1288052 unspecified role unspecified role bait bait P04637:p.[Lys319Arg;Lys320Arg;Lys321Arg;Lys370Arg;Lys372Arg;Lys373Arg;Lys381Arg;Lys382Arg;Lys386Arg] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false association association false false false Fig 5A/B p53-9KR showed reduced binding to p21 promoter as compared to WT p53 expressed at similar levels. A similar affect was observed in RNAi-mediated inhibition of Pin1. tp53-cdkn1a-10 1288046 n/a 1288052 unspecified role unspecified role bait bait P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 3D etoposide tp53-cdkn1a-13 1288046 n/a 1288052 unspecified role unspecified role bait bait P04637:p.Thr81Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false lys382 n6aclys N6-acetyl-L-lysine (2S)-6-acetamido-2-aminohexanoic acid 6-acetylamino-2-aminohexanoic acid 6-acetylazanyl-2-aminohexanoic acid MOD_RES N6-acetyllysine N(zeta)-acetyllysine N6-acetyl-L-lysine N6-acetylated L-lysine epsilon-acetyllysine Acetyl Acetylation N6AcLys acetylk certain certain sequence position certain certain certain sequence position certain false n/a 1288050 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 6B etoposide, UV. ep300-tp53-2 1288047 n/a 1288052 unspecified role unspecified role prey prey n/a 1288054 unspecified role unspecified role bait bait physical association physical association false false false Fig 4C RNAi-mediated inhibition of Pin1 expression in HCT116 cells reduced the interaction of endogenous p53 and p300 proteins. ppp1r13l-tp53-1 1288047 n/a 1288055 unspecified role unspecified role bait bait n/a 1288052 unspecified role unspecified role prey prey physical association physical association false false false Fig 7B, S7B, S8A. cytotoxic UV dosage In the absence of Pin1, the dissociation of p53 from iASPP upon cytotoxic stress was impaired. The Pin1 inhibitor PiB partially reversed the Etoposide induced dissociation of p53 from iASPP. Pin1 inhibitor - PiB iaspp-tp53-4 1288048 n/a 1288055 unspecified role unspecified role bait bait n/a 1288052 unspecified role unspecified role prey prey P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser315Ala] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig S7A ppp1r13l-tp53-2 1288048 n/a 1288052 unspecified role unspecified role prey prey P04637:p.Ser46Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false n/a 1288055 unspecified role unspecified role bait bait physical association physical association false false false Fig 7A/C/D, S8A. cytotoxic UV dosage Overexpression of catalytically active Pin1 enhanced p53 dissociation from iASPP. Disruption of single Pin1 target sites on p53 demonstrated that stress-induced dissociation of p53 from iASPP required Ser46 and, to a lesser extent, Thr81. p21-tp53 1288049 n/a 1288052 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P04637:p.Arg175His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.[Ser33Ala;Ser46Ala;Thr81Ala;Ser127Ala;Thr150Ala;Ser315Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes n/a 1288056 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig S3A lambda phosphatase Binding was observed using mutant p53 proteins with only one intact Pin1-consensus site.