IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ jahnke-2007-1 Cell death in leukemia: passenger protein regulation by topoisomerase inhibitors. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Jahnke U., Higginbottom K., Newland AC., Cotter FE., Allen PD. Apoptosis - Interactions involving proteins with a function related to apoptosis p.d.allen@qmul.ac.uk Cell cycle Biochem. Biophys. Res. Commun. (0006-291X) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer jahnke-2007-3 Cell death in leukemia: passenger protein regulation by topoisomerase inhibitors. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jahnke U., Higginbottom K., Newland AC., Cotter FE., Allen PD. Apoptosis - Interactions involving proteins with a function related to apoptosis K562 cells were untreated (control) and treated with 1 microg/ml etoposide and 20 mg/ml etoposide for 24 h and 48 h or only for 48 h. p.d.allen@qmul.ac.uk Biochem. Biophys. Res. Commun. (0006-291X) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer jahnke-2007-2 Cell death in leukemia: passenger protein regulation by topoisomerase inhibitors. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Jahnke U., Higginbottom K., Newland AC., Cotter FE., Allen PD. Apoptosis - Interactions involving proteins with a function related to apoptosis K562 cells were treated with etoposide. p.d.allen@qmul.ac.uk Biochem. Biophys. Res. Commun. (0006-291X) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA cdk1_human Cyclin-dependent kinase 1 p34 protein kinase Cell division protein kinase 1 CDK1 CDC2 Cell division control protein 2 homolog CDC28A CDKN1 P34CDC2 protein protein human Homo sapiens Human MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD ince_human Inner centromere protein INCENP protein protein human Homo sapiens Human MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAASSPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH aurkb-cdc2 Cdk1 colocalizes with Aurora kinase B at centromeres in K562 cells during prophase 667309 N/A 667312 unspecified role unspecified role neutral component neutral component N/A 667313 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 A i Prophase aurkb-cdc2-1 Cdk1 colocalizes with Aurora kinase B at centromeres in K562 cells during metaphase 667309 N/A 667312 unspecified role unspecified role neutral component neutral component N/A 667313 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 A ii Metaphase aurkb-cdc2-2 Cdk1 colocalizes with Aurora kinase B at the midzone in K562 cells during anaphase 667309 N/A 667313 unspecified role unspecified role neutral component neutral component N/A 667312 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 A iii Anaphase birc5-cdc2-3 Cdk1 colocalizes with Aurora kinase B at midbodies in K562 cells during telophase 667309 N/A 667314 unspecified role unspecified role neutral component neutral component N/A 667313 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S 3 Telophase birc5-cdc2 Cdk1 colocalizes with Survivin at centromeres in K562 cells during prophase 667309 N/A 667313 unspecified role unspecified role neutral component neutral component N/A 667314 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S 3 Prophase birc5-cdc2-1 Cdk1 colocalizes with Survivin at centromeres in K562 cells during metaphase 667309 N/A 667314 unspecified role unspecified role neutral component neutral component N/A 667313 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S 3 Metaphase birc5-cdc2-2 Cdk1 colocalizes with Survivin at the midzone in K562 cells during anaphase 667309 N/A 667314 unspecified role unspecified role neutral component neutral component N/A 667313 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S 3 Anaphase cdc2-aurkb Cdk1 coimmunoprecipitates Aurora kinase B in K562 lysates 667310 N/A 667313 unspecified role unspecified role bait bait N/A 667312 unspecified role unspecified role prey prey physical association physical association false false false 4 A c Etoposide - Treatment with etoposide increased the amount of Aurora kinase B coimmunoprecipitated with Cdk1. The level of Aurora kinase B coimmunoprecipitated with Cdk1 was increased in cells arrested at G2 and cell that undergo mitotic catastrophe. cdc2-birc5 Cdk1 coimmunoprecipitates Survivin in K562 lysates 667310 N/A 667313 unspecified role unspecified role bait bait N/A 667314 unspecified role unspecified role prey prey physical association physical association false false false 4 A d Etoposide - Treatment with etoposide increased the amount of Survivin coimmunoprecipitated with Cdk1. cdc2-incenp Cdk1 coimmunoprecipitates INCENP in K562 lysates 667310 N/A 667313 unspecified role unspecified role bait bait N/A 667315 unspecified role unspecified role prey prey physical association physical association false false false Etoposide - the level of INCENP bound to Cdk1 increased with etoposide treatment. 4 A e The level of INCENP coimmunoprecipitated with Cdk1 was increased in cells arrested at G2 and cell that undergo mitotic catastrophe. aurkb-birc5 Aurora kinase B coimmunoprecipitates Survivin from K562 lysates 667310 N/A 667314 unspecified role unspecified role prey prey N/A 667312 unspecified role unspecified role bait bait physical association physical association false false false 4 A f Etoposide - Treatment with etoposide increases the amount of Survivin coimmunoprecipitated with Aurora kinase B. Survivin binding to Aurora kinase B was increased in G2 cell cycle arrested cells and cells induced to die by DNA damage. aurkb-cdc2-3 Cdk1 colocalizes with Aurora kinase B at centromeres in K562 cells treated with etoposide 667311 N/A 667312 unspecified role unspecified role neutral component neutral component N/A 667313 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 B i and ii birc5-cdc2-4 Cdk1 colocalizes with Survivin at centromeres in K562 cells treated with etoposide 667311 N/A 667314 unspecified role unspecified role neutral component neutral component N/A 667313 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 B iii